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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Ki-67 Polyclonal Antibody
catalog :
PA5-19462
quantity :
100 µg
price :
US 496.00
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
African green monkey, human, mouse, domestic rabbit
application :
immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
citations: 72
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry - frozen section; African green monkey; 1:200-1:2000; loading ...; fig 1g
  • immunohistochemistry - frozen section; human; 1:200-1:2000; fig 5d
Zhu X, Guo Y, Chu C, Liu D, Duan K, Yin Y, et al. BRN2 as a key gene drives the early primate telencephalon development. Sci Adv. 2022;8:eabl7263 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; fig 1
Liang Y, Zhu F, Zhang H, Chen D, Zhang X, Gao Q, et al. Conditional ablation of TGF-? signaling inhibits tumor progression and invasion in an induced mouse bladder cancer model. Sci Rep. 2016;6:29479 pubmed publisher
  • immunohistochemistry - paraffin section; mouse; 1:50; fig 1
Chen G, Luo Y, Eriksson D, Meng X, Qian C, Bauerle T, et al. High fat diet increases melanoma cell growth in the bone marrow by inducing osteopontin and interleukin 6. Oncotarget. 2016;7:26653-69 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig s3
Malanga D, Belmonte S, Colelli F, Scarfò M, De Marco C, Oliveira D, et al. AKT1E¹⁷K Is Oncogenic in Mouse Lung and Cooperates with Chemical Carcinogens in Inducing Lung Cancer. PLoS ONE. 2016;11:e0147334 pubmed publisher
  • immunohistochemistry; mouse; 1:100; fig 5
Wu Z, Li D, Huang Y, Chen X, Huang W, Liu C, et al. Caspr Controls the Temporal Specification of Neural Progenitor Cells through Notch Signaling in the Developing Mouse Cerebral Cortex. Cereb Cortex. 2017;27:1369-1385 pubmed publisher
Luo Z, Li Z, Liang Z, Wang L, He G, Wang D, et al. Berberine increases stromal production of Wnt molecules and activates Lgr5+ stem cells to promote epithelial restitution in experimental colitis. BMC Biol. 2022;20:287 pubmed publisher
Jing X, Wu J, Dong C, Gao J, Seki T, Kim C, et al. COVID-19 instigates adipose browning and atrophy through VEGF in small mammals. Nat Metab. 2022;4:1674-1683 pubmed publisher
Bayram P, Billur D, Kizil S, Caliskan H, Can B. Alterations in hippocampal neurogenesis and hippocampal insulin signaling pathway in rat with metabolic syndrome. Iran J Basic Med Sci. 2022;25:1308-1316 pubmed publisher
Caravia X, Ramirez Martinez A, Gan P, Wang F, McAnally J, Xu L, et al. Loss of function of the nuclear envelope protein LEMD2 causes DNA damage-dependent cardiomyopathy. J Clin Invest. 2022;132: pubmed publisher
El Sayed F, Nassef E, Abdelmageed N, Abdel Maqsoud R, Abosaif A. Diffusion-Weighted MRI as Non-Invasive Diagnostic Tool for Rectal Cancer Aggressiveness and Correlation with KI-67 Expression in Tumor Tissue. Asian Pac J Cancer Prev. 2022;23:3387-3391 pubmed publisher
Wang J, Sun X, Wang J, Zhang K, Yuan Y, Guo Y, et al. NDRG2 inhibits pyruvate carboxylase-mediated anaplerosis and combines with glutamine blockade to inhibit the proliferation of glioma cells. Am J Cancer Res. 2022;12:3729-3744 pubmed
Azevedo A, Pais A, Almeida Santos T, Pires V, Pessa P, Marques C, et al. Medical Grade Honey as a Promising Treatment to Improve Ovarian Tissue Transplantation. Bioengineering (Basel). 2022;9: pubmed publisher
Seki T, Yang Y, Sun X, Lim S, Xie S, Guo Z, et al. Brown-fat-mediated tumour suppression by cold-altered global metabolism. Nature. 2022;608:421-428 pubmed publisher
Lan C, Chen C, Qu S, Cao N, Luo H, Yu C, et al. Inhibition of DYRK1A, via histone modification, promotes cardiomyocyte cell cycle activation and cardiac repair after myocardial infarction. EBioMedicine. 2022;82:104139 pubmed publisher
Poh A, Love C, Chisanga D, Steer J, Baloyan D, Chopin M, et al. Therapeutic inhibition of the SRC-kinase HCK facilitates T cell tumor infiltration and improves response to immunotherapy. Sci Adv. 2022;8:eabl7882 pubmed publisher
Welty S, Thathiah A, Levine A. DNA Damage Increases Secreted Aβ40 and Aβ42 in Neuronal Progenitor Cells: Relevance to Alzheimer's Disease. J Alzheimers Dis. 2022;88:177-190 pubmed publisher
Yin W, Liontos A, Koepke J, Ghoul M, Mazzocchi L, Liu X, et al. An essential function for autocrine hedgehog signaling in epithelial proliferation and differentiation in the trachea. Development. 2022;149: pubmed publisher
Feng C, Wang Q, Deng L, Peng N, Yang M, Wang X. Hsa_circ_0074032 promotes prostate cancer progression through elevating homeobox A1 expression by serving as a microRNA-198 decoy. Andrologia. 2022;54:e14312 pubmed publisher
Gong X, Tian M, Cao N, Yang P, Xu Z, Zheng S, et al. Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation. J Clin Invest. 2021;131: pubmed publisher
Vohlen C, Mohr J, Fomenko A, Kuiper Makris C, Grzembke T, Aydogmus R, et al. Dynamic Regulation of GH-IGF1 Signaling in Injury and Recovery in Hyperoxia-Induced Neonatal Lung Injury. Cells. 2021;10: pubmed publisher
Pais A, Reis S, Laranjo M, Caramelo F, Silva F, Botelho M, et al. The challenge of ovarian tissue culture: 2D versus 3D culture. J Ovarian Res. 2021;14:147 pubmed publisher
Bellinger D, Dulcich M, Molinaro C, Gifford P, Lorton D, Gridley D, et al. Psychosocial Stress and Age Influence Depression and Anxiety-Related Behavior, Drive Tumor Inflammatory Cytokines and Accelerate Prostate Cancer Growth in Mice. Front Oncol. 2021;11:703848 pubmed publisher
Bunma T, Kanjanaruch C, Kogram N, Uriyapongson S, Khanthusaeng V, Navanukraw C. Effects of FSH treatment and withdrawal during proestrus on uterine proliferation and steroid hormone receptor expression in beef heifers. Anim Sci J. 2021;92:e13621 pubmed publisher
Tutuianu R, Rosca A, Iacomi D, Simionescu M, Titorencu I. Human Mesenchymal Stromal Cell-Derived Exosomes Promote In Vitro Wound Healing by Modulating the Biological Properties of Skin Keratinocytes and Fibroblasts and Stimulating Angiogenesis. Int J Mol Sci. 2021;22: pubmed publisher
Yu Q, Biswas S, Ma G, Zhao P, Li B, Li J. Canonical NF-κB signaling maintains corneal epithelial integrity and prevents corneal aging via retinoic acid. elife. 2021;10: pubmed publisher
Liu L, Cui J, Zhao Y, Liu X, Chen L, Xia Y, et al. KDM6A-ARHGDIB axis blocks metastasis of bladder cancer by inhibiting Rac1. Mol Cancer. 2021;20:77 pubmed publisher
Ma B, Ren G, Xu J, Yin C, Shi Y. LncRNA MNX1-AS1 Contributes to Laryngeal Squamous Cell Carcinoma Growth and Migration by Regulating mir-744-5p/bcl9/β-Catenin Axis. Cell Transplant. 2021;30:9636897211005682 pubmed publisher
Amen A, Fellmann C, Soczek K, Ren S, Lew R, Knott G, et al. Cancer-specific loss of TERT activation sensitizes glioblastoma to DNA damage. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
Galego S, Kauppila L, Malhó R, Pimentel J, Brito M. Myocyte Enhancer Factor 2C as a New Player in Human Breast Cancer Brain Metastases. Cells. 2021;10: pubmed publisher
Xiang P, Yeung Y, Wang J, Wu Q, Du R, Huang C, et al. miR-17-3p promotes the proliferation of multiple myeloma cells by downregulating P21 expression through LMLN inhibition. Int J Cancer. 2021;148:3071-3085 pubmed publisher
Wang X, Hu X, Song W, Xu H, Xiao Z, Huang R, et al. Mutual dependency between lncRNA LETN and protein NPM1 in controlling the nucleolar structure and functions sustaining cell proliferation. Cell Res. 2021;31:664-683 pubmed publisher
Gan S, Su C, Ma J, Liu M, Cui X, Xin L, et al. Translation of Tudor-SN, a novel terminal oligo-pyrimidine (TOP) mRNA, is regulated by the mTORC1 pathway in cardiomyocytes. RNA Biol. 2021;18:900-913 pubmed publisher
Li K, Varden L, Henderson A, Lufkin T, Kraus P. Simultaneous detection of multiple mRNAs and proteins in bovine IVD cells and tissue with single cell resolution. Biotechnol Lett. 2021;43:13-24 pubmed publisher
Rajabian N, Shahini A, Asmani M, Vydiam K, Choudhury D, Nguyen T, et al. Bioengineered Skeletal Muscle as a Model of Muscle Aging and Regeneration. Tissue Eng Part A. 2021;27:74-86 pubmed publisher
Nasiri B, Row S, Smith R, Swartz D, Andreadis S. Cell-free vascular grafts that grow with the host. Adv Funct Mater. 2020;30: pubmed publisher
Hosaka K, Yang Y, Seki T, Du Q, Jing X, He X, et al. Therapeutic paradigm of dual targeting VEGF and PDGF for effectively treating FGF-2 off-target tumors. Nat Commun. 2020;11:3704 pubmed publisher
Senatus L, López Díez R, Egaña Gorroño L, Liu J, Hu J, Daffu G, et al. RAGE impairs murine diabetic atherosclerosis regression and implicates IRF7 in macrophage inflammation and cholesterol metabolism. JCI Insight. 2020;5: pubmed publisher
Shoneye T, Orrego A, Jarvis R, Men Y, Chiang M, Yang Y. Differential Proliferation and Maturation of Subcortical Astrocytes During Postnatal Development. Front Neurosci. 2020;14:435 pubmed publisher
McKnight S, Simmons R, Wu G, Satterfield M. Maternal arginine supplementation enhances thermogenesis in the newborn lamb. J Anim Sci. 2020;98: pubmed publisher
Fallah M, Viklund E, Bäckman A, Broden J, Lundskog B, Johansson M, et al. Plasminogen is a master regulator and a potential drug candidate for the healing of radiation wounds. Cell Death Dis. 2020;11:201 pubmed publisher
Calvani M, Bruno G, Dabraio A, Subbiani A, Bianchini F, Fontani F, et al. β3-Adrenoreceptor Blockade Induces Stem Cells Differentiation in Melanoma Microenvironment. Int J Mol Sci. 2020;21: pubmed publisher
Morrison V, Smith V, Huang J. Retinoic Acid Is Required for Oligodendrocyte Precursor Cell Production and Differentiation in the Postnatal Mouse Corpus Callosum. Eneuro. 2020;7: pubmed publisher
Huang R, Yuan D, Li S, Liang X, Gao Y, Lan X, et al. NCAM regulates temporal specification of neural progenitor cells via profilin2 during corticogenesis. J Cell Biol. 2020;219: pubmed publisher
Wang L, Shen E, Luo L, Rabe H, Wang Q, Yin J, et al. Control of Germinal Center Localization and Lineage Stability of Follicular Regulatory T Cells by the Blimp1 Transcription Factor. Cell Rep. 2019;29:1848-1861.e6 pubmed publisher
Ishak G, Dutra G, Gastal G, Gastal M, Feugang J, Gastal E. Transition to the ovulatory season in mares: An investigation of antral follicle receptor gene expression in vivo. Mol Reprod Dev. 2019;86:1832-1845 pubmed publisher
Xian P, Hei Y, Wang R, Wang T, Yang J, Li J, et al. Mesenchymal stem cell-derived exosomes as a nanotherapeutic agent for amelioration of inflammation-induced astrocyte alterations in mice. Theranostics. 2019;9:5956-5975 pubmed publisher
Mensching L, Djogo N, Keller C, Rading S, Karsak M. Stable Adult Hippocampal Neurogenesis in Cannabinoid Receptor CB2 Deficient Mice. Int J Mol Sci. 2019;20: pubmed publisher
Méndez A, Rojas D, Ponce C, Bustamante R, Beltrán C, Toledo J, et al. Primary infection by Pneumocystis induces Notch-independent Clara cell mucin production in rat distal airways. PLoS ONE. 2019;14:e0217684 pubmed publisher
Xie X, Zu X, Liu F, Wang T, Wang X, Chen H, et al. Purpurogallin is a novel mitogen-activated protein kinase kinase 1/2 inhibitor that suppresses esophageal squamous cell carcinoma growth in vitro and in vivo. Mol Carcinog. 2019;58:1248-1259 pubmed publisher
Lv Q, Wang G, Zhang Y, Han X, Li H, Le W, et al. FABP5 regulates the proliferation of clear cell renal cell carcinoma cells via the PI3K/AKT signaling pathway. Int J Oncol. 2019;54:1221-1232 pubmed publisher
Baboota R, Shinde A, Lemaire K, Fransen M, Vinckier S, Van Veldhoven P, et al. Functional peroxisomes are required for β-cell integrity in mice. Mol Metab. 2019;: pubmed publisher
Papoff G, Presutti D, Lalli C, Bolasco G, Santini S, Manelfi C, et al. CASP4 gene silencing in epithelial cancer cells leads to impairment of cell migration, cell-matrix adhesion and tissue invasion. Sci Rep. 2018;8:17705 pubmed publisher
Lewinska A, Bocian A, Petrilla V, Adamczyk Grochala J, Szymura K, Hendzel W, et al. Snake venoms promote stress-induced senescence in human fibroblasts. J Cell Physiol. 2019;234:6147-6160 pubmed publisher
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Lin M, Wang Y, Wu W, Wolseley V, Tsai M, Radic V, et al. Zika Virus Infects Intermediate Progenitor Cells and Post-mitotic Committed Neurons in Human Fetal Brain Tissues. Sci Rep. 2017;7:14883 pubmed publisher
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Lin C, Chen Y, Lin C, Chen Y, Lo G, Lee P, et al. Amiodarone as an autophagy promoter reduces liver injury and enhances liver regeneration and survival in mice after partial hepatectomy. Sci Rep. 2015;5:15807 pubmed publisher
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image
image 1 :
Invitrogen PA5-19462 image 1
Western blot analysis of HeLa Whole Cell Lysate using PA5-19462, Ki67 primary antibody at a dilution of 1ug/ml. Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.
product information
Product Type :
Antibody
Product Name :
Ki-67 Polyclonal Antibody
Catalog # :
PA5-19462
Quantity :
100 µg
Price :
US 496.00
Clonality :
Polyclonal
Purity :
Antigen affinity chromatography
Host :
Rabbit
Reactivity :
Human, Mouse, Rabbit
Applications :
Immunocytochemistry: 0.5-1 µg/ml, Immunohistochemistry (Paraffin): 0.1 - 5 µg/mL
Species :
Human, Mouse, Rabbit
Isotype :
IgG
Storage :
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Description :
Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.
Immunogen :
Synthetic peptide conjugated to KLH derived from within residues 1200 - 1300 of Human Ki67.
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 0.5-1 µg/ml, Immunohistochemistry (Paraffin): 0.1 - 5 µg/mL
Aliases :
antigen identified by monoclonal antibody Ki 67; antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; antigen KI-67; proliferation marker protein Ki-67; antigen KI-67-like; cb31; D630048A14Rik; I79_022666; Ki67; Ki-67; KIA; LOW QUALITY PROTEIN: proliferation marker protein Ki-67; marker of proliferation Ki-67; MIB-; MIB-1; Mki67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; sb:cb31; si:ch211-250b22.7; unnamed protein product; wu:fa11g09; wu:fb57a07; wu:fi14e05
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA