TSC1 Polyclonal Antibody | Invitrogen PA5-18506 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

TSC1 Polyclonal Antibody

catalog :

PA5-18506

quantity :

100 ug

price :

US 474.00

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunocytochemistry knockout validation

more info or order :

Invitrogen product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry knockout validation; mouse; 1:200; fig 1 western blot; mouse; 1:200; fig 1	Jiang M, Liu L, He X, Wang H, Lin W, Wang H, et al. Regulation of PERK-eIF2? signalling by tuberous sclerosis complex-1 controls homoeostasis and survival of myelinating oligodendrocytes. Nat Commun. 2016;7:12185 pubmed publisher
	Jiang M, Rao R, Wang J, Wang J, Xu L, Wu L, et al. The TSC1-mTOR-PLK axis regulates the homeostatic switch from Schwann cell proliferation to myelination in a stage-specific manner. Glia. 2018;66:1947-1959 pubmed publisher

product information

Product Type :

Antibody

Product Name :

TSC1 Polyclonal Antibody

Catalog # :

PA5-18506

Quantity :

100 ug

Price :

US 474.00

Clonality :

Polyclonal

Purity :

Ammonium sulfate precipitation

Host :

Goat

Reactivity :

Human

Applications :

Immunohistochemistry (Paraffin): 2-4 ug/mL, Western Blot: 0.1-0.3 ug/mL

Species :

Human

Isotype :

IgG

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.

Immunogen :

Synthetic peptide sequence (QLHIMDYNETHHEH) corresponding to the C-terminus amino acids of TSC1 (aa 1150-1164).

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 2-4 ug/mL, Western Blot: 0.1-0.3 ug/mL

Aliases :

hamartin; HGNC:12362; Kiaa0243; LAM; MGC86987; OTTHUMP00000022439; OTTHUMP00000198378; TSC; Tsc1; tuberous sclerosis 1; tuberous sclerosis 1 protein; Tuberous sclerosis 1 protein homolog; tumor suppressor

more info or order :

Invitrogen product webpage