PCK1 Polyclonal Antibody | Invitrogen PA5-120718 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

PCK1 Polyclonal Antibody

catalog :

PA5-120718

quantity :

100 uL

price :

US 460.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation

product information

Product Type :

Antibody

Product Name :

PCK1 Polyclonal Antibody

Catalog # :

PA5-120718

Quantity :

100 uL

Price :

US 460.00

Clonality :

Polyclonal

Purity :

Affinity Chromatography

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Applications :

ELISA: 1 ug/mL, Immunocytochemistry: 1:50-1:200, Immunoprecipitation: 0.5 ug-4 ug antibody for 400 ug-600 ug extracts of, Western Blot: 1:500-1:1,000

Species :

Human, Mouse, Rat

Isotype :

IgG

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

Phosphoenolpyruvate Carboxykinase 1 (PCK1) is a main control point for the regulation of gluconeogenesis. During gluconeogenesis PCK1 acts as the rate-limiting enzyme. PCK1 regulates the formation and maintenance of memory CH8(+) T-cells via gluconeogenesis. PCK1 aids in controlling the levels of metabolic intermediates in the citric acid cycle by regulating cataplerosis and anaplerosis. When glucose levels are high, PCK1 catalyzes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (PEP). When they are low it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate. When phosphorylated at Ser-90 by AKT1, PCK1 acts as a protein kinase by reducing the binding affinity to oxaloacetate and promotes an atypical serine protein kinase activity using GTP as the donor. This activity regulates lipogenesis by disrupting the interaction between INSIG proteins and SCAP and promoting nuclear translocation of SREBP proteins and transcription of lipogenesis-related genes. An important paralog of this gene is PCK2.

Immunogen :

Recombinant fusion protein containing a sequence corresponding to amino acids 490-622 of human PCK1 (NP_002582.3).

Format :

Liquid

Applications w/Dilutions :

ELISA: 1 ug/mL, Immunocytochemistry: 1:50-1:200, Immunoprecipitation: 0.5 ug-4 ug antibody for 400 ug-600 ug extracts of, Western Blot: 1:500-1:1,000

Aliases :

AI265463; GTP; HGNC:8724; MGC22652; PCK; Pck1; Pck-1; PE PEPCKC; PEP carboxykinase; PEPCK; PEPCK1; PEPCKC; PEPCK-C; phosphoenolpyruvate carboxykinase (GTP); phosphoenolpyruvate carboxykinase 1; phosphoenolpyruvate carboxykinase 1 (soluble); phosphoenolpyruvate carboxykinase 1, cytosolic; phosphoenolpyruvate carboxykinase, cytosolic; phosphoenolpyruvate carboxykinase, cytosolic (GTP); phosphoenolpyruvate carboxykinase, cytosolic [GTP]; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase; RATPEPCK