DNase I Polyclonal Antibody | Invitrogen PA5-119083 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

DNase I Polyclonal Antibody

catalog :

PA5-119083

quantity :

200 uL

price :

US 434.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section

product information

Product Type :

Antibody

Product Name :

DNase I Polyclonal Antibody

Catalog # :

PA5-119083

Quantity :

200 uL

Price :

US 434.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human

Applications :

Immunohistochemistry (Paraffin): 5-20 ug/mL, Western Blot: 0.01-2 ug/mL

Species :

Human

Isotype :

IgG

Storage :

-20 C

Description :

Internucleosomal DNA fragmentation following the activation of endonucleases is the common end point of apoptosis. DNase I, a Ca2+/Mg2+-dependent endonuclease ubiquitously expressed in mammalian tissues, has been implicated to mediate internucleosomal DNA degradation in human cells undergoing apoptosis. DNase I is highly polymorphic, and at least six alleles of DNase I are known. DNase II, the ubiquitously expressed acidic deoxyribonuclease, acts downstream of caspase activation and may also induce DNA digestion during apoptosis. DNase I cleaves DNA to 5'-phosphodinucleotide and 5'-phospho- oligonucleotide end-products, whereas DNase II cleaves DNA to 3'-phosphomononucleotide and 3'-phosphooligonucleotide end-products. The mechanism by which DNase II cuts DNA is similar to DNase I, which produces nicks rather than double-strand cuts. DNase II is usually present in cytoplasm of epithelial cells, but it appears concentrated in the nuclei of lens fibers. In contrast, DNase I is always concentrated in nuclei of epithelial and fiber cells.

Immunogen :

Recombinant protein Deoxyribonuclease I. The antigen corresponds to amino acid range 19-259 of the target protein.

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 5-20 ug/mL, Western Blot: 0.01-2 ug/mL

Aliases :

AI788650; deoxyribonuclease; Deoxyribonuclease 1; deoxyribonuclease I; Deoxyribonuclease1; Deoxyribonuclease-1; DeoxyribonucleaseI; DNASE 1; DNase I; DNase I lysosomal; DNase I, lysosomal; Dnase1; DNaseI; DNL 1; DNL1; Dornase alfa; DRNI; epithelial lens deoxyribonuclease I; FLJ38093; human urine deoxyribonuclease I; pancreatic deoxyribonuclease I