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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Calreticulin Polyclonal Antibody
catalog :
PA3-900
quantity :
100 uL
price :
US 496.00
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
African green monkey, human, mouse, rat, dogs, domestic rabbit
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry
more info or order :
citations: 74
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; loading ...; fig 3g
Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht M, et al. Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum. Neuron. 2021;109:299-313.e9 pubmed publisher
  • immunocytochemistry; human; 1:300; loading ...; fig 5a
Wang G, Xu J, Zhao J, Yin W, Liu D, Chen W, et al. Arf1-mediated lipid metabolism sustains cancer cells and its ablation induces anti-tumor immune responses in mice. Nat Commun. 2020;11:220 pubmed publisher
  • western blot; human; fig 4c
Wang W, Wang J, Lin W, Kao C, Hung M, Teng Y, et al. Progerin in muscle leads to thermogenic and metabolic defects via impaired calcium homeostasis. Aging Cell. 2020;19:e13090 pubmed publisher
  • immunocytochemistry; African green monkey; loading ...; fig 5e
Tiwarekar V, Fehrholz M, Schneider Schaulies J. KDELR2 Competes with Measles Virus Envelope Proteins for Cellular Chaperones Reducing Their Chaperone-Mediated Cell Surface Transport. Viruses. 2019;11: pubmed publisher
  • flow cytometry; human; fig 10b
Granato M, Rizzello C, Gilardini Montani M, Cuomo L, Vitillo M, Santarelli R, et al. Quercetin induces apoptosis and autophagy in primary effusion lymphoma cells by inhibiting PI3K/AKT/mTOR and STAT3 signaling pathways. J Nutr Biochem. 2017;41:124-136 pubmed publisher
  • immunocytochemistry; human; loading ...; fig 3a
Ishimura R, Obata M, Kageyama S, Daniel J, Tanaka K, Komatsu M. A novel approach to assess the ubiquitin-fold modifier 1-system in cells. FEBS Lett. 2017;591:196-204 pubmed publisher
  • western blot; human; fig 4b
Greenwood E, Matheson N, Wals K, van den Boomen D, Antrobus R, Williamson J, et al. Temporal proteomic analysis of HIV infection reveals remodelling of the host phosphoproteome by lentiviral Vif variants. elife. 2016;5: pubmed publisher
  • immunocytochemistry; human; 1:100; loading ...; fig 1b
Greotti E, Wong A, Pozzan T, Pendin D, Pizzo P. Characterization of the ER-Targeted Low Affinity Ca(2+) Probe D4ER. Sensors (Basel). 2016;16: pubmed publisher
  • western blot; human; fig 1
Liu C, LeClair P, Monajemi M, Sly L, Reid G, Lim C. α-Integrin expression and function modulates presentation of cell surface calreticulin. Cell Death Dis. 2016;7:e2268 pubmed publisher
  • western blot; mouse; 1:1000; fig 3
Mosca B, Eckhardt J, Bergamelli L, Treves S, Bongianino R, De Negri M, et al. Role of the JP45-Calsequestrin Complex on Calcium Entry in Slow Twitch Skeletal Muscles. J Biol Chem. 2016;291:14555-65 pubmed publisher
  • immunocytochemistry; mouse; fig 2a
Shih Y, Hsueh Y. VCP and ATL1 regulate endoplasmic reticulum and protein synthesis for dendritic spine formation. Nat Commun. 2016;7:11020 pubmed publisher
  • flow cytometry; mouse; fig 2
Tagliamonte M, Petrizzo A, Napolitano M, Luciano A, Rea D, Barbieri A, et al. A novel multi-drug metronomic chemotherapy significantly delays tumor growth in mice. J Transl Med. 2016;14:58 pubmed publisher
  • immunohistochemistry; mouse; 1:1000; fig 8
Ruegsegger C, Stucki D, Steiner S, Angliker N, Radecke J, Keller E, et al. Impaired mTORC1-Dependent Expression of Homer-3 Influences SCA1 Pathophysiology. Neuron. 2016;89:129-46 pubmed publisher
Zarate S, Huntington T, Bagher P, Srinivasan R. Aging reduces calreticulin expression and alters spontaneous calcium signals in astrocytic endfeet of the mouse dorsolateral striatum. NPJ Aging. 2023;9:5 pubmed publisher
Bushey R, Saxena R, Campa M, Gottlin E, He Y, Patz E. Antitumor Immune Mechanisms of the Anti-Complement Factor H Antibody GT103. Mol Cancer Ther. 2023;22:778-789 pubmed publisher
Yao H, Zhang S, Xie H, Fan Y, Miao M, Zhu R, et al. RCN2 promotes Nasopharyngeal carcinoma progression by curbing Calcium flow and Mitochondrial apoptosis. Cell Oncol (Dordr). 2023;46:1031-1048 pubmed publisher
Lores S, G xe1 mez Chiachio M, Cascallar M, Ramos Nebot C, Hurtado P, Alijas S, et al. Effectiveness of a novel gene nanotherapy based on putrescine for cancer treatment. Biomater Sci. 2023;11:4210-4225 pubmed publisher
Xu C, Huang J, Jiang Y, He S, Zhang C, Pu K. Nanoparticles with ultrasound-induced afterglow luminescence for tumour-specific theranostics. Nat Biomed Eng. 2023;7:298-312 pubmed publisher
Zhang R, Neighbors J, Schell T, Hohl R. Schweinfurthin induces ICD without ER stress and caspase activation. Oncoimmunology. 2022;11:2104551 pubmed publisher
Lin Y, Huang Y, Wang M, Chen C, Chen W, Weng Y, et al. Calreticulin Regulates β1-Integrin mRNA Stability in PC-3 Prostate Cancer Cells. Biomedicines. 2022;10: pubmed publisher
Haney S, Varney M, Williams J, Smith L, Talmon G, Holstein S. Geranylgeranyl diphosphate synthase inhibitor and proteasome inhibitor combination therapy in multiple myeloma. Exp Hematol Oncol. 2022;11:5 pubmed publisher
Hagemann C, Moreno Gonzalez C, Guetta L, Tyzack G, Chiappini C, Legati A, et al. Axonal Length Determines Distinct Homeostatic Phenotypes in Human iPSC Derived Motor Neurons on a Bioengineered Platform. Adv Healthc Mater. 2022;11:e2101817 pubmed publisher
Abdullah T, Whatmore J, Bremer E, Slibinskas R, Michalak M, Eggleton P. Endoplasmic reticulum stress-induced release and binding of calreticulin from human ovarian cancer cells. Cancer Immunol Immunother. 2022;71:1655-1669 pubmed publisher
Cali T, Brini M. Quantification of organelle contact sites by split-GFP-based contact site sensors (SPLICS) in living cells. Nat Protoc. 2021;16:5287-5308 pubmed publisher
Bai X, Zhou Y, Lin Q, Huang C, Zhang S, Carlson R, et al. Bio-nanoparticle based therapeutic vaccine induces immunogenic response against triple negative breast cancer. Am J Cancer Res. 2021;11:4141-4174 pubmed
Sampieri L, Funes Chab xe1 n M, Di Giusto P, Roz xe9 s Salvador V, Alvarez C. CREB3L2 Modulates Nerve Growth Factor-Induced Cell Differentiation. Front Mol Neurosci. 2021;14:650338 pubmed publisher
Coukos R, Yao D, Sánchez M, Strand E, Olive M, Udeshi N, et al. An engineered transcriptional reporter of protein localization identifies regulators of mitochondrial and ER membrane protein trafficking in high-throughput CRISPRi screens. elife. 2021;10: pubmed publisher
Tukaramrao D, Malla S, Saraiya S, Hanely R, Ray A, Kumari S, et al. A Novel Thienopyrimidine Analog, TPH104, Mediates Immunogenic Cell Death in Triple-Negative Breast Cancer Cells. Cancers (Basel). 2021;13: pubmed publisher
Venkatesan A, Geng J, Kandarpa M, Wijeyesakere S, Bhide A, Talpaz M, et al. Mechanism of mutant calreticulin-mediated activation of the thrombopoietin receptor in cancers. J Cell Biol. 2021;220: pubmed publisher
Pavlou M, Sch xf6 n C, Occelli L, Rossi A, Meumann N, Boyd R, et al. Novel AAV capsids for intravitreal gene therapy of photoreceptor disorders. EMBO Mol Med. 2021;13:e13392 pubmed publisher
Theodoridis P, Bokros M, Marijan D, Balukoff N, Wang D, Kirk C, et al. Local translation in nuclear condensate amyloid bodies. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
Hatstat A, Ahrendt H, Foster M, Mayne L, Moseley M, Englander S, et al. Characterization of Small-Molecule-Induced Changes in Parkinson's-Related Trafficking via the Nedd4 Ubiquitin Signaling Cascade. Cell Chem Biol. 2021;28:14-25.e9 pubmed publisher
Mączyńska J, Da Pieve C, Burley T, Raes F, Shah A, Saczko J, et al. Immunomodulatory activity of IR700-labelled affibody targeting HER2. Cell Death Dis. 2020;11:886 pubmed publisher
Mohan H, Yang B, Dean N, Raghavan M. Calreticulin enhances the secretory trafficking of a misfolded α-1-antitrypsin. J Biol Chem. 2020;295:16754-16772 pubmed publisher
Mei K, Liao Y, Jiang J, Chiang M, Khazaieli M, Liu X, et al. Liposomal Delivery of Mitoxantrone and a Cholesteryl Indoximod Prodrug Provides Effective Chemo-immunotherapy in Multiple Solid Tumors. ACS Nano. 2020;14:13343-13366 pubmed publisher
Di Grazia A, Laudisi F, Di Fusco D, Franzè E, Ortenzi A, Monteleone I, et al. Rafoxanide Induces Immunogenic Death of Colorectal Cancer Cells. Cancers (Basel). 2020;12: pubmed publisher
Mozzi A, Biolatti M, Cagliani R, Forni D, Dell Oste V, Pontremoli C, et al. Past and ongoing adaptation of human cytomegalovirus to its host. PLoS Pathog. 2020;16:e1008476 pubmed publisher
Furlan S, Campione M, Murgia M, Mosole S, Argenton F, Volpe P, et al. Calsequestrins New Calcium Store Markers of Adult Zebrafish Cerebellum and Optic Tectum. Front Neuroanat. 2020;14:15 pubmed publisher
Kopecka J, Godel M, Dei S, Giampietro R, Belisario D, Akman M, et al. Insights into P-Glycoprotein Inhibitors: New Inducers of Immunogenic Cell Death. Cells. 2020;9: pubmed publisher
Biwer L, Askew Page H, Hong K, Milstein J, Johnstone S, Macal E, et al. Endothelial calreticulin deletion impairs endothelial function in aged mice. Am J Physiol Heart Circ Physiol. 2020;318:H1041-H1048 pubmed publisher
Suzuki E, Ogawa N, Takeda T, Nishito Y, Tanaka Y, Fujiwara T, et al. Detailed analyses of the crucial functions of Zn transporter proteins in alkaline phosphatase activation. J Biol Chem. 2020;295:5669-5684 pubmed publisher
Seyedin S, Hasibuzzaman M, Pham V, Petronek M, Callaghan C, Kalen A, et al. Combination Therapy with Radiation and PARP Inhibition Enhances Responsiveness to Anti-PD-1 Therapy in Colorectal Tumor Models. Int J Radiat Oncol Biol Phys. 2020;108:81-92 pubmed publisher
Ma J, Ramachandran M, Jin C, Quijano Rubio C, Martikainen M, Yu D, et al. Characterization of virus-mediated immunogenic cancer cell death and the consequences for oncolytic virus-based immunotherapy of cancer. Cell Death Dis. 2020;11:48 pubmed publisher
Benfield C, MacKenzie F, Ritzefeld M, Mazzon M, Weston S, Tate E, et al. Bat IFITM3 restriction depends on S-palmitoylation and a polymorphic site within the CD225 domain. Life Sci Alliance. 2020;3: pubmed publisher
Tiscione S, Vivas O, Ginsburg K, Bers D, Ory D, Santana L, et al. Disease-associated mutations in Niemann-Pick type C1 alter ER calcium signaling and neuronal plasticity. J Cell Biol. 2019;218:4141-4156 pubmed publisher
Liu C, Leclair P, Pedari F, Vieira H, Monajemi M, Sly L, et al. Integrins and ERp57 Coordinate to Regulate Cell Surface Calreticulin in Immunogenic Cell Death. Front Oncol. 2019;9:411 pubmed publisher
Anwar T, Liu X, Suntio T, Marjamäki A, Biazik J, Chan E, et al. ER-Targeted Beclin 1 Supports Autophagosome Biogenesis in the Absence of ULK1 and ULK2 Kinases. Cells. 2019;8: pubmed publisher
Rossi A, Dupaty L, Aillot L, Zhang L, Gallien C, Hallek M, et al. Vector uncoating limits adeno-associated viral vector-mediated transduction of human dendritic cells and vector immunogenicity. Sci Rep. 2019;9:3631 pubmed publisher
Pandya U, Egbuta C, Abdullah Norman T, Chiang C, Wiersma V, Panchal R, et al. The Biophysical Interaction of the Danger-Associated Molecular Pattern (DAMP) Calreticulin with the Pattern-Associated Molecular Pattern (PAMP) Lipopolysaccharide. Int J Mol Sci. 2019;20: pubmed publisher
Champion L, Pawar S, Luithle N, Ungricht R, Kutay U. Dissociation of membrane-chromatin contacts is required for proper chromosome segregation in mitosis. Mol Biol Cell. 2019;30:427-440 pubmed publisher
Chorro L, Suzuki M, Chin S, Williams T, Snapp E, Odagiu L, et al. Interleukin 2 modulates thymic-derived regulatory T cell epigenetic landscape. Nat Commun. 2018;9:5368 pubmed publisher
Menzies S, Volkmar N, van den Boomen D, Timms R, Dickson A, Nathan J, et al. The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1. elife. 2018;7: pubmed publisher
Salaroglio I, Gazzano E, Abdullrahman A, Mungo E, Castella B, Abd Elrahman G, et al. Increasing intratumor C/EBP-β LIP and nitric oxide levels overcome resistance to doxorubicin in triple negative breast cancer. J Exp Clin Cancer Res. 2018;37:286 pubmed publisher
Takeda T, Miyazaki S, Kobayashi M, Nishino K, Goto T, Matsunaga M, et al. Zinc deficiency causes delayed ATP clearance and adenosine generation in rats and cell culture models. Commun Biol. 2018;1:113 pubmed publisher
Plaud C, Joshi V, Kajevu N, Poüs C, Curmi P, Burgo A. Functional differences of short and long isoforms of spastin harboring missense mutation. Dis Model Mech. 2018;11: pubmed publisher
Li L, Jiao X, D Atri I, Ono F, Nelson R, Chan C, et al. Mutation in the intracellular chloride channel CLCC1 associated with autosomal recessive retinitis pigmentosa. PLoS Genet. 2018;14:e1007504 pubmed publisher
Vigneron N, Ferrari V, van den Eynde B, Cresswell P, Leonhardt R. Cytosolic Processing Governs TAP-Independent Presentation of a Critical Melanoma Antigen. J Immunol. 2018;201:1875-1888 pubmed publisher
Hangen E, Cordelières F, Petersen J, Choquet D, Coussen F. Neuronal Activity and Intracellular Calcium Levels Regulate Intracellular Transport of Newly Synthesized AMPAR. Cell Rep. 2018;24:1001-1012.e3 pubmed publisher
Arshad N, Cresswell P. Tumor-associated calreticulin variants functionally compromise the peptide loading complex and impair its recruitment of MHC-I. J Biol Chem. 2018;293:9555-9569 pubmed publisher
Borrelli C, Ricci B, Vulpis E, Fionda C, Ricciardi M, Petrucci M, et al. Drug-Induced Senescent Multiple Myeloma Cells Elicit NK Cell Proliferation by Direct or Exosome-Mediated IL15 Trans-Presentation. Cancer Immunol Res. 2018;6:860-869 pubmed publisher
Xing X, Mroß C, Hao L, Munck M, Herzog A, Mohr C, et al. Nesprin-2 Interacts with Condensin Component SMC2. Int J Cell Biol. 2017;2017:8607532 pubmed publisher
Cieri D, Vicario M, Giacomello M, Vallese F, Filadi R, Wagner T, et al. SPLICS: a split green fluorescent protein-based contact site sensor for narrow and wide heterotypic organelle juxtaposition. Cell Death Differ. 2018;25:1131-1145 pubmed publisher
Wang Y, Xie J, Liu Z, Fu H, Huo Q, Gu Y, et al. Association of calreticulin expression with disease activity and organ damage in systemic lupus erythematosus patients. Exp Ther Med. 2017;13:2577-2583 pubmed publisher
Doghman Bouguerra M, Granatiero V, Sbiera S, Sbiera I, Lacas Gervais S, Brau F, et al. FATE1 antagonizes calcium- and drug-induced apoptosis by uncoupling ER and mitochondria. EMBO Rep. 2016;17:1264-80 pubmed publisher
Wijeyesakere S, Gagnon J, Arora K, Brooks C, Raghavan M. Regulation of calreticulin-major histocompatibility complex (MHC) class I interactions by ATP. Proc Natl Acad Sci U S A. 2015;112:E5608-17 pubmed publisher
Granato M, Gilardini Montani M, Filardi M, Faggioni A, Cirone M. Capsaicin triggers immunogenic PEL cell death, stimulates DCs and reverts PEL-induced immune suppression. Oncotarget. 2015;6:29543-54 pubmed publisher
Williamson C, Wong D, Bozidis P, Zhang A, COLBERG POLEY A. Isolation of Endoplasmic Reticulum, Mitochondria, and Mitochondria-Associated Membrane and Detergent Resistant Membrane Fractions from Transfected Cells and from Human Cytomegalovirus-Infected Primary Fibroblasts. Curr Protoc Cell Biol. 2015;68:3.27.1-33 pubmed publisher
Parkinson M, Piper S, Bright N, Evans J, Boname J, Bowers K, et al. A non-canonical ESCRT pathway, including histidine domain phosphotyrosine phosphatase (HD-PTP), is used for down-regulation of virally ubiquitinated MHC class I. Biochem J. 2015;471:79-88 pubmed publisher
Matsuno H, Ohi K, Hashimoto R, Yamamori H, Yasuda Y, Fujimoto M, et al. A naturally occurring null variant of the NMDA type glutamate receptor NR3B subunit is a risk factor of schizophrenia. PLoS ONE. 2015;10:e0116319 pubmed publisher
Tafaleng E, Chakraborty S, Han B, Hale P, Wu W, Soto Gutierrez A, et al. Induced pluripotent stem cells model personalized variations in liver disease resulting from α1-antitrypsin deficiency. Hepatology. 2015;62:147-57 pubmed publisher
Filézac de L Etang A, Maharjan N, Cordeiro Braña M, Ruegsegger C, Rehmann R, Goswami A, et al. Marinesco-Sjögren syndrome protein SIL1 regulates motor neuron subtype-selective ER stress in ALS. Nat Neurosci. 2015;18:227-38 pubmed publisher
Turpin J, Journo C, Ko N, Sinet F, Carpentier A, Galioot A, et al. Discovery and characterization of auxiliary proteins encoded by type 3 simian T-cell lymphotropic viruses. J Virol. 2015;89:931-51 pubmed publisher
Bernard Marissal N, Sunyach C, Marissal T, Raoul C, Pettmann B. Calreticulin levels determine onset of early muscle denervation by fast motoneurons of ALS model mice. Neurobiol Dis. 2015;73:130-6 pubmed publisher
Pearse B, Gabriel L, Wang N, Hebert D. A cell-based reglucosylation assay demonstrates the role of GT1 in the quality control of a maturing glycoprotein. J Cell Biol. 2008;181:309-20 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Calreticulin Polyclonal Antibody
Catalog # :
PA3-900
Quantity :
100 uL
Price :
US 496.00
Clonality :
Polyclonal
Host :
Rabbit
Reactivity :
Canine, Human, Mouse, Non-human primate, Rabbit, Rat
Applications :
Flow Cytometry: Assay-dependent, Immunocytochemistry: 1:50 - 1:200, Immunohistochemistry: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000
Species :
Canine, Human, Mouse, Non-human primate, Rabbit, Rat
Isotype :
IgG
Storage :
-20 C, Avoid Freeze/Thaw Cycles
Description :
Calr or Calreticulin is a calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. Calr, a lectin, interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Calr also interacts with with the DNA-binding domain of NR3C1 and mediates its nuclear export. It may also be involved in maternal gene expression regulation and oocyte maturation via the regulation of calcium homeostasis.
Immunogen :
Recombinant, human calreticulin protein.
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: Assay-dependent, Immunocytochemistry: 1:50 - 1:200, Immunohistochemistry: Assay-dependent, Immunoprecipitation: Assay-dependent, Western Blot: 1:1,000
Aliases :
Autoantigen RO; CABP3; CALBP; calcium-binding protein 3; CALR; calrectulin; calregulin; Calreticulin; calreticulin precursor; cC1qR; CRP55; CRT; CRTC; Endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 99n; ERp60; FLJ26680; grp60; HACBP; HEL-S-99n; I79_008346; RO; Ro/SS-A Antigen; Sicca syndrome antigen A (autoantigen Ro; calreticulin); SSA
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA