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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
HIF1A Polyclonal Antibody
catalog :
PA3-16521
quantity :
100 uL
price :
US 474.00
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
guinea pig, human, mouse, rat, dogs, bovine, zebrafish , Xenopus laevis
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
Invitrogen product webpage
citations: 3
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:500; loading ...; fig 9a
Djamali A, Wilson N, Sadowski E, Zha W, Niles D, Hafez O, et al. Nox2 and Cyclosporine-Induced Renal Hypoxia. Transplantation. 2016;100:1198-210 pubmed publisher
Kumar S, Suman S, Moon B, Fornace A, Datta K. Low dose radiation upregulates Ras/p38 and NADPH oxidase in mouse colon two months after exposure. Mol Biol Rep. 2023;50:2067-2076 pubmed publisher
Zhang Y, Yan X, Xu S, Pang Z, Li L, Yang Y, et al. α-Lipoic Acid Maintains Brain Glucose Metabolism via BDNF/TrkB/HIF-1α Signaling Pathway in P301S Mice. Front Aging Neurosci. 2020;12:262 pubmed publisher
product information
Product Type :
Antibody
Product Name :
HIF1A Polyclonal Antibody
Catalog # :
PA3-16521
Quantity :
100 uL
Price :
US 474.00
Clonality :
Polyclonal
Purity :
Antigen affinity chromatography
Host :
Rabbit
Reactivity :
Bovine, Canine, Guinea Pig, Human, Mouse, Non-human primate, Rat, Xenopus laevis, Zebrafish
Applications :
ChIP Assay: 2.5 ug/10^6 cells, ChIP-Sequencing: Assay-Dependent, ELISA: Assay-Dependent, Gel Shift: 1:100, Immunocytochemistry: 1:100-1:500, Immunohistochemistry (Frozen): 1:100-1:500, Immunohistochemistry (Paraffin): 1:100-1:500, Immunoprecipitation: 1:1,000, Western Blot: 1:500-1:1,000
Species :
Bovine, Canine, Guinea Pig, Human, Mouse, Non-human primate, Rat, Xenopus laevis, Zebrafish
Isotype :
IgG
Storage :
Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.
Description :
HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.
Immunogen :
Fusion protein containing amino acids 432-528 of human HIF-1alpha
Format :
Liquid
Applications w/Dilutions :
ChIP Assay: 2.5 ug/10^6 cells, ChIP-Sequencing: Assay-Dependent, ELISA: Assay-Dependent, Gel Shift: 1:100, Immunocytochemistry: 1:100-1:500, Immunohistochemistry (Frozen): 1:100-1:500, Immunohistochemistry (Paraffin): 1:100-1:500, Immunoprecipitation: 1:1,000, Western Blot: 1:500-1:1,000
Aliases :
AA959795; ARNT interacting protein; ARNT2; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF; hif 1; hif 1a; HIF1; HIF1 alpha; HIF-1 alpha; HIF1a; hif-1a; hif1a.L; HIF1alpha; HIF-1alpha; HIF-1-alpha; HIF1-alpha; HIF1-alpha protein; hypoxia inducible factor 1 alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1 alpha subunit L homeolog; hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1 subunit alpha L homeolog; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia inducible factor-1 alpha; hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1 alpha subunit; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) L homeolog; hypoxia-inducible factor 1-alpha; hypoxia-inducible factor-1 alpha; hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8; XELAEV_18039792mg; XELAEV_18041137mg; xhif1a
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA