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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
iNOS Polyclonal Antibody
catalog :
PA3-030A
quantity :
100 uL
price :
US 520.00
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
Invitrogen product webpage
citations: 60
Published Application/Species/Sample/DilutionReference
  • immunohistochemistry; mouse; 1:200; fig 4c
Poudel S, Dixit M, Yildirim G, Cordoba Chacon J, Gahete M, Yuji I, et al. Sexual dimorphic impact of adult-onset somatopause on life span and age-induced osteoarthritis. Aging Cell. 2021;20:e13427 pubmed publisher
  • western blot; human; loading ...; fig 6a
Lee S, Kim J, Choi Y, Gong J, Park S, Douangdeuane B, et al. Therapeutic Effects of Dipterocarpus tuberculatus with High Antioxidative Activity Against UV-Induced Photoaging of NHDF Cells and Nude Mice. Antioxidants (Basel). 2021;10: pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 3e
Li T, Zhang Z, Bartolacci J, Dwyer G, Liu Q, Mathews L, et al. Graft IL-33 regulates infiltrating macrophages to protect against chronic rejection. J Clin Invest. 2020;130:5397-5412 pubmed publisher
  • immunohistochemistry - frozen section; mouse; loading ...; fig 4d
Srinivasan S, Su M, Ravishankar S, Moore J, Head P, Dixon J, et al. TLR-exosomes exhibit distinct kinetics and effector function. Sci Rep. 2017;7:41623 pubmed publisher
  • western blot; mouse; 1:2000; loading ...; fig 4e
Yang X, Huo F, Liu B, Liu J, Chen T, Li J, et al. Crocin Inhibits Oxidative Stress and Pro-inflammatory Response of Microglial Cells Associated with Diabetic Retinopathy Through the Activation of PI3K/Akt Signaling Pathway. J Mol Neurosci. 2017;61:581-589 pubmed publisher
  • immunohistochemistry; human; loading ...; fig s1b
Vidimar V, Gius D, Chakravarti D, Bulun S, Wei J, Kim J. Dysfunctional MnSOD leads to redox dysregulation and activation of prosurvival AKT signaling in uterine leiomyomas. Sci Adv. 2016;2:e1601132 pubmed
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 1c
Fan T, Warmoes M, Sun Q, Song H, Turchan Cholewo J, Martin J, et al. Distinctly perturbed metabolic networks underlie differential tumor tissue damages induced by immune modulator ?-glucan in a two-case ex vivo non-small-cell lung cancer study. Cold Spring Harb Mol Case Stud. 2016;2:a000893 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:100; loading ...
Michel H, Tadros M, Esmat A, Khalifa A, Abdel tawab A. Tetramethylpyrazine Ameliorates Rotenone-Induced Parkinson's Disease in Rats: Involvement of Its Anti-Inflammatory and Anti-Apoptotic Actions. Mol Neurobiol. 2017;54:4866-4878 pubmed publisher
  • western blot; mouse; fig 4
Chen L, Teng H, Fang T, Xiao J. Agrimonolide from Agrimonia pilosa suppresses inflammatory responses through down-regulation of COX-2/iNOS and inactivation of NF-?B in lipopolysaccharide-stimulated macrophages. Phytomedicine. 2016;23:846-55 pubmed publisher
  • immunohistochemistry - paraffin section; rat; 1:200; fig 2
Sönmez M, Dündar M. Ameliorative effects of pentoxifylline on NOS induced by diabetes in rat kidney. Ren Fail. 2016;38:605-13 pubmed publisher
  • western blot; rat; 1:1000; fig 4
Wu C, Chou H, Huang L, Lin Y, Chen C. Bubble CPAP Support after Discontinuation of Mechanical Ventilation Protects Rat Lungs with Ventilator-Induced Lung Injury. Respiration. 2016;91:171-9 pubmed publisher
  • immunohistochemistry; rat; 1:200; fig 2
  • western blot; rat; 1:1000; fig 3
Sönmez M, Kılıç E, Karabulut D, Çilenk K, Deligönül E, Dündar M. Nitric oxide synthase in diabetic rat testicular tissue and the effects of pentoxifylline therapy. Syst Biol Reprod Med. 2016;62:22-30 pubmed publisher
Baniya P, Tebyani M, Asefifeyzabadi N, Nguyen T, Hernández C, Zhu K, et al. A system for bioelectronic delivery of treatment directed toward wound healing. Sci Rep. 2023;13:14766 pubmed publisher
Dawood A, Sedeek M, Farag M, Abdelnaser A. Terfezia boudieri and Terfezia claveryi inhibit the LPS/IFN-γ-mediated inflammation in RAW 264.7 macrophages through an Nrf2-independent mechanism. Sci Rep. 2023;13:10106 pubmed publisher
Kamble M, Saadi F, Kumar S, Saha B, Das Sarma J. Inducible nitric oxide synthase deficiency promotes murine-β-coronavirus induced demyelination. Virol J. 2023;20:51 pubmed publisher
Kwesiga M, Gillette A, Razaviamri F, Plank M, Canull A, Alesch Z, et al. Biodegradable magnesium materials regulate ROS-RNS balance in pro-inflammatory macrophage environment. Bioact Mater. 2023;23:261-273 pubmed publisher
Chow L, Soontararak S, Wheat W, Ammons D, Dow S. Canine polarized macrophages express distinct functional and transcriptomic profiles. Front Vet Sci. 2022;9:988981 pubmed publisher
Feng T, Zhao X, Gu P, Yang W, Wang C, Guo Q, et al. Adipocyte-derived lactate is a signalling metabolite that potentiates adipose macrophage inflammation via targeting PHD2. Nat Commun. 2022;13:5208 pubmed publisher
Ding Y, Liu X, Zhang J, Lv Z, Meng X, Yuan Z, et al. 3D printing polylactic acid polymer-bioactive glass loaded with bone cement for bone defect in weight-bearing area. Front Bioeng Biotechnol. 2022;10:947521 pubmed publisher
Johnson V, Chow L, Harrison J, Soontararak S, Dow S. Activated Mesenchymal Stromal Cell Therapy for Treatment of Multi-Drug Resistant Bacterial Infections in Dogs. Front Vet Sci. 2022;9:925701 pubmed publisher
Zhang Y, Li S, Jin P, Shang T, Sun R, Lu L, et al. Dual functions of microRNA-17 in maintaining cartilage homeostasis and protection against osteoarthritis. Nat Commun. 2022;13:2447 pubmed publisher
Cottler P, Kang H, Nash V, Salopek L, Bruce A, Spiller K, et al. Immunomodulation of Acellular Dermal Matrix Through Interleukin 4 Enhances Vascular Infiltration. Ann Plast Surg. 2022;88:S466-S472 pubmed publisher
Kung W, Lin C, Chen W, Jiang L, Sun Y, Hsieh K, et al. Anti-Inflammatory CDGSH Iron-Sulfur Domain 2: A Biomarker of Central Nervous System Insult in Cellular, Animal Models and Patients. Biomedicines. 2022;10: pubmed publisher
Abramowitz L, Hanover J. Chronically Elevated O-GlcNAcylation Limits Nitric Oxide Production and Deregulates Specific Pro-Inflammatory Cytokines. Front Immunol. 2022;13:802336 pubmed publisher
Kang J, Lee J, Seol I, Kim Y, Park M, Yoo H. Pharmacological Effects of Gami-Yukmijihwang-Tang on the Lipopolysaccharide-Induced Hippocampus Oxidation and Inflammation via Regulation of Sirt6. Pharmaceuticals (Basel). 2022;15: pubmed publisher
Tang R, Cao Q, Hu S, He L, Du P, Chen G, et al. Sulforaphane activates anti-inflammatory microglia, modulating stress resilience associated with BDNF transcription. Acta Pharmacol Sin. 2022;43:829-839 pubmed publisher
Khare S, Kim L, Lobel G, Doulias P, Ischiropoulos H, Nissim I, et al. ASS1 and ASL suppress growth in clear cell renal cell carcinoma via altered nitrogen metabolism. Cancer Metab. 2021;9:40 pubmed publisher
Kanda K, Asawa Y, Inaki R, Fujihara Y, Hoshi K, Hikita A. Requirement of direct contact between chondrocytes and macrophages for the maturation of regenerative cartilage. Sci Rep. 2021;11:22476 pubmed publisher
Li W, Lin S, Chu C, Chang Y, Zhang X, Lin C, et al. The Gastroprotective Effect of Naringenin against Ethanol-Induced Gastric Ulcers in Mice through Inhibiting Oxidative and Inflammatory Responses. Int J Mol Sci. 2021;22: pubmed publisher
Lai J, Hsu Y, Pan T, Lee C. Monascin and Ankaflavin of Monascus purpureus Prevent Alcoholic Liver Disease through Regulating AMPK-Mediated Lipid Metabolism and Enhancing Both Anti-Inflammatory and Anti-Oxidative Systems. Molecules. 2021;26: pubmed publisher
Niu Y, Stadler F, Yang X, Deng F, Liu G, Xia H. HA-coated collagen nanofibers for urethral regeneration via in situ polarization of M2 macrophages. J Nanobiotechnology. 2021;19:283 pubmed publisher
Kuo C, Yang T, Tsai P, Yu C. Role of the Inflammatory Response of RAW 264.7 Cells in the Metastasis of Novel Cancer Stem-Like Cells. Medicina (Kaunas). 2021;57: pubmed publisher
Schröder A, Stumpf J, Paddenberg E, Neubert P, Schatz V, Kostler J, et al. Effects of mechanical strain on periodontal ligament fibroblasts in presence of Aggregatibacter actinomycetemcomitans lysate. BMC Oral Health. 2021;21:405 pubmed publisher
Gokina N, Fairchild R, Prakash K, DeLance N, Bonney E. Deficiency in CD4 T Cells Leads to Enhanced Postpartum Internal Carotid Artery Vasoconstriction in Mice: The Role of Nitric Oxide. Front Physiol. 2021;12:686429 pubmed publisher
Zhou Q, Katano M, Zhang J, Liu X, Wang K, Iinuma M, et al. Chewing Behavior Attenuates the Tumor Progression-Enhancing Effects of Psychological Stress in a Breast Cancer Model Mouse. Brain Sci. 2021;11: pubmed publisher
Sadozai H, Acharjee A, Gruber T, Gloor B, Karamitopoulou E. Pancreatic Cancers with High Grade Tumor Budding Exhibit Hallmarks of Diminished Anti-Tumor Immunity. Cancers (Basel). 2021;13: pubmed publisher
Sadozai H, Acharjee A, Eppenberger Castori S, Gloor B, Gruber T, Schenk M, et al. Distinct Stromal and Immune Features Collectively Contribute to Long-Term Survival in Pancreatic Cancer. Front Immunol. 2021;12:643529 pubmed publisher
Xu Y, Zhang Y, Liang H, Liu X. Coumestrol mitigates retinal cell inflammation, apoptosis, and oxidative stress in a rat model of diabetic retinopathy via activation of SIRT1. Aging (Albany NY). 2021;13:5342-5357 pubmed publisher
Lin M. CISD2 Attenuates Inflammation and Regulates Microglia Polarization in EOC Microglial Cells-As a Potential Therapeutic Target for Neurodegenerative Dementia. Front Aging Neurosci. 2020;12:260 pubmed publisher
Bayrak S, Aktas S, Altun Z, Cakir Y, Tutuncu M, Kum Özşengezer S, et al. Antioxidant effect of acetyl-l-carnitine against cisplatin-induced cardiotoxicity. J Int Med Res. 2020;48:300060520951393 pubmed publisher
Yeo I, Yun J, Son D, Han S, Hong J. Antifungal drug miconazole ameliorated memory deficits in a mouse model of LPS-induced memory loss through targeting iNOS. Cell Death Dis. 2020;11:623 pubmed publisher
Vené R, Costa D, Augugliaro R, Carlone S, Scabini S, Casoni Pattacini G, et al. Evaluation of Glycosylated PTGS2 in Colorectal Cancer for NSAIDS-Based Adjuvant Therapy. Cells. 2020;9: pubmed publisher
Abdel Aziz A, Naguib Abdel Hafez S. Sitagliptin protects male albino rats with testicular ischaemia/reperfusion damage: Modulation of VCAM-1 and VEGF-A. Andrologia. 2020;52:e13472 pubmed publisher
Kuthati Y, Goutham Davuluri V, Yang C, Chang H, Chang C, Wong C. Melatonin MT2 receptor agonist IIK-7 produces antinociception by modulation of ROS and suppression of spinal microglial activation in neuropathic pain rats. J Pain Res. 2019;12:2473-2485 pubmed publisher
Shen Q, Li J, Chan D, Sandy J, Takeuchi J, Ross R, et al. Effect of intra-articular hyaluronan injection on inflammation and bone remodeling in the epiphyses and metaphyses of the knee in a murine model of joint injury. Am J Transl Res. 2019;11:3280-3300 pubmed
Pentecost A, Kim M, Jeon S, Ko Y, Kwon I, Gogotsi Y, et al. Immunomodulatory nanodiamond aggregate-based platform for the treatment of rheumatoid arthritis. Regen Biomater. 2019;6:163-174 pubmed publisher
Niu J, Tsai H, Hoi K, Huang N, Yu G, Kim K, et al. Aberrant oligodendroglial-vascular interactions disrupt the blood-brain barrier, triggering CNS inflammation. Nat Neurosci. 2019;22:709-718 pubmed publisher
Gill N, Leng Y, Romero R, Xu Y, Panaitescu B, Miller D, et al. The immunophenotype of decidual macrophages in acute atherosis. Am J Reprod Immunol. 2019;81:e13098 pubmed publisher
Ugolini F, Lana D, Nardiello P, Nosi D, Pantano D, Casamenti F, et al. Different Patterns of Neurodegeneration and Glia Activation in CA1 and CA3 Hippocampal Regions of TgCRND8 Mice. Front Aging Neurosci. 2018;10:372 pubmed publisher
Qian Y, Song J, Sun P, Yi R, Liu H, Feng X, et al. Lactobacillus casei Strain Shirota Enhances the In Vitro Antiproliferative Effect of Geniposide in Human Oral Squamous Carcinoma HSC-3 Cells. Molecules. 2018;23: pubmed publisher
Alpizar Y, Boonen B, Sanchez A, Jung C, Lopez Requena A, Naert R, et al. TRPV4 activation triggers protective responses to bacterial lipopolysaccharides in airway epithelial cells. Nat Commun. 2017;8:1059 pubmed publisher
Brandt A, Jin C, Nolte K, Sellmann C, Engstler A, Bergheim I. Short-Term Intake of a Fructose-, Fat- and Cholesterol-Rich Diet Causes Hepatic Steatosis in Mice: Effect of Antibiotic Treatment. Nutrients. 2017;9: pubmed publisher
Fang M, Yuan Y, Rangarajan P, Lu J, Wu Y, Wang H, et al. Scutellarin regulates microglia-mediated TNC1 astrocytic reaction and astrogliosis in cerebral ischemia in the adult rats. BMC Neurosci. 2015;16:84 pubmed publisher
Karabulut D, Ulusoy H, Kaymak E, Sonmez M. Therapeutic effects of pentoxifylline on diabetic heart tissue via NOS. Anatol J Cardiol. 2016;16:310-5 pubmed publisher
Hasan S, Sultana S. Geraniol attenuates 2-acetylaminofluorene induced oxidative stress, inflammation and apoptosis in the liver of wistar rats. Toxicol Mech Methods. 2015;25:559-73 pubmed publisher
Sun J, Yang Y, Zhang Y, Huang W, Li Z, Zhang Y. Minocycline attenuates pain by inhibiting spinal microglia activation in diabetic rats. Mol Med Rep. 2015;12:2677-82 pubmed publisher
Li W, Cai L, Zhang Y, Cui L, Shen G. Intra-articular resveratrol injection prevents osteoarthritis progression in a mouse model by activating SIRT1 and thereby silencing HIF-2α. J Orthop Res. 2015;33:1061-70 pubmed publisher
Menze E, Esmat A, Tadros M, Abdel Naim A, Khalifa A. Genistein improves 3-NPA-induced memory impairment in ovariectomized rats: impact of its antioxidant, anti-inflammatory and acetylcholinesterase modulatory properties. PLoS ONE. 2015;10:e0117223 pubmed publisher
Ammar A, Esmat A, Hassona M, Tadros M, Abdel Naim A, Guns E. The effect of pomegranate fruit extract on testosterone-induced BPH in rats. Prostate. 2015;75:679-92 pubmed publisher
Frontera Acevedo K, Sakamoto K. Local pulmonary immune responses in domestic cats naturally infected with Cytauxzoon felis. Vet Immunol Immunopathol. 2015;163:1-7 pubmed publisher
product information
Product Type :
Antibody
Product Name :
iNOS Polyclonal Antibody
Catalog # :
PA3-030A
Quantity :
100 uL
Price :
US 520.00
Clonality :
Polyclonal
Host :
Rabbit
Reactivity :
Human, Mouse
Applications :
Immunocytochemistry: 1:250, Immunohistochemistry (Frozen): 1:500, Immunohistochemistry (Paraffin): 1:200, Western Blot: 1:2,000
Species :
Human, Mouse
Isotype :
IgG
Storage :
-20 C, Avoid Freeze/Thaw Cycles
Description :
iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.
Immunogen :
Synthetic peptide corresponding to residues C K(1131) K G S A L E E P K A T R L(1144) of mouse macrophage NOS.
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 1:250, Immunohistochemistry (Frozen): 1:500, Immunohistochemistry (Paraffin): 1:200, Western Blot: 1:2,000
Aliases :
Hepatocyte NOS; hepatocytes; HEP-NOS; inducible nitric oxide synthase; inducible NO synthase; Inducible NOS; iNos; i-NOS; Inosl; MAC-NOS; macrophage NOS; nitric oxide synthase 2; nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase, inducible; nitric oxide synthase, macrophage; nitric oxide synthase-inducible; NOS; NOS type II; NOS, type II; Nos2; Nos-2; Nos2a; NOS-II; OTTMUSP00000000202; peptidyl-cysteine S-nitrosylase NOS2
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA