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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
GAPDH Polyclonal Antibody
catalog :
PA1-988
quantity :
100 µg
price :
US 380.00
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat, dogs, bovine
application :
western blot
more info or order :
Invitrogen product webpage
citations: 28
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:1000; loading ...; fig 1
Longo F, Mercatelli D, Novello S, Arcuri L, Brugnoli A, Vincenzi F, et al. Age-dependent dopamine transporter dysfunction and Serine129 phospho-α-synuclein overload in G2019S LRRK2 mice. Acta Neuropathol Commun. 2017;5:22 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 4c
De Los Santos S, García Pérez V, Hernández Reséndiz S, Palma Flores C, González Gutiérrez C, Zazueta C, et al. (-)-Epicatechin induces physiological cardiac growth by activation of the PI3K/Akt pathway in mice. Mol Nutr Food Res. 2017;61: pubmed publisher
  • western blot; bovine; fig 2
Koch F, Lamp O, Eslamizad M, Weitzel J, Kuhla B. Metabolic Response to Heat Stress in Late-Pregnant and Early Lactation Dairy Cows: Implications to Liver-Muscle Crosstalk. PLoS ONE. 2016;11:e0160912 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 1d
Gong K, Qu B, Liao D, Liu D, Wang C, Zhou J, et al. MiR-132 regulates osteogenic differentiation via downregulating Sirtuin1 in a peroxisome proliferator-activated receptor ?/?-dependent manner. Biochem Biophys Res Commun. 2016;478:260-267 pubmed publisher
  • western blot; mouse; 1:1000; loading ...; fig 1h
Qu B, Ma Y, Yan M, Gong K, Liang F, Deng S, et al. Sirtuin1 promotes osteogenic differentiation through downregulation of peroxisome proliferator-activated receptor ? in MC3T3-E1 cells. Biochem Biophys Res Commun. 2016;478:439-445 pubmed publisher
  • western blot; human
Haven B, Heilig E, Donham C, Settles M, Vasilevsky N, Owen K. Registered report: A chromatin-mediated reversible drug-tolerant state in cancer cell subpopulations. elife. 2016;5: pubmed publisher
Liu P, Li X, Liu J, Zhang H, You Z, Zhang J. Cacna2d2 inhibits axonal regeneration following surgical decompression in a rat model of cervical spondylotic myelopathy. BMC Neurosci. 2022;23:42 pubmed publisher
Del Giudice R, Imbimbo P, Pietrocola F, Martins I, De Palma F, Bravo San Pedro J, et al. Autophagy Alteration in ApoA-I Related Systemic Amyloidosis. Int J Mol Sci. 2022;23: pubmed publisher
Costa Verdera H, Collaud F, Riling C, Sellier P, Nordin J, Preston G, et al. Hepatic expression of GAA results in enhanced enzyme bioavailability in mice and non-human primates. Nat Commun. 2021;12:6393 pubmed publisher
Zhang X, Li J, Feng Q. CircRNA circYY1 (hsa_circ_0101187) Modulates Cell Glycolysis and Malignancy Through Regulating YY1 Expression by Sponging miR-769-3p in Breast Cancer. Cancer Manag Res. 2021;13:1145-1158 pubmed publisher
Li Y, Gong H, Feng L, Mao D, Xiao Y, Wang Y, et al. Chondroitin polymerizing factor promotes breast carcinoma cell proliferation, invasion and migration and affects expression of epithelial-mesenchymal transition-related markers. FEBS Open Bio. 2021;11:423-434 pubmed publisher
Yang J, Cao H, Guo S, Zhu H, Tao H, Zhang L, et al. Small molecular compounds efficiently convert human fibroblasts directly into neurons. Mol Med Rep. 2020;22:4763-4771 pubmed publisher
Colella P, Sellier P, Gómez M, Biferi M, Tanniou G, Guerchet N, et al. Gene therapy with secreted acid alpha-glucosidase rescues Pompe disease in a novel mouse model with early-onset spinal cord and respiratory defects. EBioMedicine. 2020;61:103052 pubmed publisher
Zhang Y, Wang J, Yin H, Wang S, He C, Liu J. DACH1, a novel target of miR-218, participates in the regulation of cell viability, apoptosis, inflammatory response, and epithelial-mesenchymal transition process in renal tubule cells treated by high-glucose. Ren Fail. 2020;42:463-473 pubmed publisher
Deshpande A, Vinayakamoorthy R, Garg B, Thummapudi J, Oza G, Adhikari K, et al. Why Does Knocking Out NACHO, But Not RIC3, Completely Block Expression of α7 Nicotinic Receptors in Mouse Brain?. Biomolecules. 2020;10: pubmed publisher
Amadi I, Agrawal V, Christianson T, Bardliving C, Shamlou P, LeBowitz J. Inhibition of endogenous miR-23a/miR-377 in CHO cells enhances difficult-to-express recombinant lysosomal sulfatase activity. Biotechnol Prog. 2020;36:e2974 pubmed publisher
Laforet P, Inoue M, Goillot E, Lefeuvre C, Cagin U, Streichenberger N, et al. Deep morphological analysis of muscle biopsies from type III glycogenesis (GSDIII), debranching enzyme deficiency, revealed stereotyped vacuolar myopathy and autophagy impairment. Acta Neuropathol Commun. 2019;7:167 pubmed publisher
Chakravarthy M, Veedu R. BACE1 Inhibition Using 2'-OMePS Steric Blocking Antisense Oligonucleotides. Genes (Basel). 2019;10: pubmed publisher
Salazar G, Cullen A, Huang J, Zhao Y, Serino A, Hilenski L, et al. SQSTM1/p62 and PPARGC1A/PGC-1alpha at the interface of autophagy and vascular senescence. Autophagy. 2020;16:1092-1110 pubmed publisher
Yuan M, Wang Y, Qin Y. Engineered nanomedicine for neuroregeneration: light emitting diode-mediated superparamagnetic iron oxide-gold core-shell nanoparticles functionalized by nerve growth factor. Nanomedicine. 2019;21:102052 pubmed publisher
Colella P, Sellier P, Costa Verdera H, Puzzo F, Van Wittenberghe L, Guerchet N, et al. AAV Gene Transfer with Tandem Promoter Design Prevents Anti-transgene Immunity and Provides Persistent Efficacy in Neonate Pompe Mice. Mol Ther Methods Clin Dev. 2019;12:85-101 pubmed publisher
Brewer A, Liu S, Buhler A, Shirachi D, Quock R. Role of spinal GABA receptors in the acute antinociceptive response of mice to hyperbaric oxygen. Brain Res. 2018;1699:107-116 pubmed publisher
Yuan L, Singh D, Buescher J, Arikkath J. A role for proteolytic regulation of δ-catenin in remodeling a subpopulation of dendritic spines in the rodent brain. J Biol Chem. 2018;293:11625-11638 pubmed publisher
Li J. Downregulation of ROS1 enhances the therapeutic efficacy of arsenic trioxide in acute myeloid leukemia cell lines. Oncol Lett. 2018;15:9392-9396 pubmed publisher
Connor M, Pulsifer A, CHUNG D, Rouchka E, Ceresa B, Lawrenz M. Yersinia pestis Targets the Host Endosome Recycling Pathway during the Biogenesis of the Yersinia-Containing Vacuole To Avoid Killing by Macrophages. MBio. 2018;9: pubmed publisher
Garg B, Loring R. Evaluating Commercially Available Antibodies for Rat α7 Nicotinic Acetylcholine Receptors. J Histochem Cytochem. 2017;65:499-512 pubmed publisher
Goutzourelas N, Stagos D, Housmekeridou A, Karapouliou C, Kerasioti E, Aligiannis N, et al. Grape pomace extract exerts antioxidant effects through an increase in GCS levels and GST activity in muscle and endothelial cells. Int J Mol Med. 2015;36:433-41 pubmed publisher
Verduzco D, Lloyd M, Xu L, Ibrahim Hashim A, Balagurunathan Y, Gatenby R, et al. Intermittent hypoxia selects for genotypes and phenotypes that increase survival, invasion, and therapy resistance. PLoS ONE. 2015;10:e0120958 pubmed publisher
product information
Product Type :
Antibody
Product Name :
GAPDH Polyclonal Antibody
Catalog # :
PA1-988
Quantity :
100 µg
Price :
US 380.00
Clonality :
Polyclonal
Purity :
Antigen affinity chromatography
Host :
Rabbit
Reactivity :
Canine, Human, Mouse, Non-human primate, Rat
Applications :
Western Blot: 1:500-1:5,000
Species :
Canine, Human, Mouse, Non-human primate, Rat
Isotype :
IgG
Storage :
-20° C, Avoid Freeze/Thaw Cycles
Description :
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.
Immunogen :
Synthetic peptide: V(4) K V G V N G F G R I G R L V(18)
Format :
Liquid
Applications w/Dilutions :
Western Blot: 1:500-1:5,000
Aliases :
38 kDa BFA-dependent ADP-ribosylation substrate; aging-associated gene 9 protein; BARS-38; bb02e05; cb350; cb609; CDABP0047; EC 1.2.1.12; epididymis secretory protein Li 278; epididymis secretory sperm binding protein Li 162eP; fb71f08; fk58c09; G3PD; G3PDH; GAPD; GAPD2; gapdh; GAPDH2; GAPDH-2; GAPDHS; Gapds; Gapd-s; glceraldehyde-3-phosphate dehydrogenase; glyceraldehyde 3-phosphate dehydrogenase; glyceraldehyde 3-phosphate dehydrogenase, testis-specific; glyceraldehyde phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase (G3PDH); glyceraldehyde-3-phosphate dehydrogenase 2; glyceraldehyde-3-phosphate dehydrogenase GAPDH; glyceraldehyde-3-phosphate dehydrogenase like-17 protein; glyceraldehyde-3-phosphate dehydrogenase type 2; glyceraldehyde-3-phosphate dehydrogenase, spermatogenic; glyceraldehyde-3-phosphate dehydrogenase, testis-specific; glyceraldehyde-phosphate-dehydrogenase; glycerine aldehyde 3-phosphate dehydrogenase; HEL-S-162eP; HEL-S-278; HGNC:4141; HSD35; HSD-35; I79_001391; KNC-NDS6; LOW QUALITY PROTEIN: glyceraldehyde-3-phosphate dehydrogenase, testis-specific; mg:bb02e05; MGC128279 protein; MGC88685; multifunctional protein, glycolytic enzyme; OK/SW-cl.12; Peptidyl-cysteine S-nitrosylase GAPDH; similar to glyceraldehyde 3-phosphate dehydrogenase; spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2; Spermatogenic glyceraldehyde-3-phosphate dehydrogenase; Unknown (protein for IMAGE:8101613); unnamed protein product; wu:fb33a10; wu:fb71f08; wu:fk58c09; wu:ft80f05; zgc:76908
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA