DYKDDDDK Tag Polyclonal Antibody, HRP | Invitrogen PA1-984B-HRP product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

DYKDDDDK Tag Polyclonal Antibody, HRP

catalog :

PA1-984B-HRP

quantity :

50 uL

price :

US 394.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

HRP

application :

western blot

citations: 8

Reference
Fielden L, Scott N, Palmer C, Khoo C, Newton H, Stojanovski D. Proteomic Identification of Coxiella burnetii Effector Proteins Targeted to the Host Cell Mitochondria During Infection. Mol Cell Proteomics. 2020;20:100005 pubmed publisher
Tetenborg S, Wang H, Nemitz L, Depping A, Espejo A, Aseervatham J, et al. Phosphorylation of Connexin36 near the C-terminus switches binding affinities for PDZ-domain and 14-3-3 proteins in vitro. Sci Rep. 2020;10:18378 pubmed publisher
Li Y, Duah E, Long N, Persaud A, Vangundy Z, MAGLIERY T, et al. An efficient and quantitative assay for epitope-tagged therapeutic protein development with a capillary western system. Bioanalysis. 2019;11:471-483 pubmed publisher
Georges A, Marcon E, Greenblatt J, Frappier L. Identification and Characterization of USP7 Targets in Cancer Cells. Sci Rep. 2018;8:15833 pubmed publisher
Frick P, Sellier C, Mackenzie I, Cheng C, Tahraoui Bories J, Martinat C, et al. Novel antibodies reveal presynaptic localization of C9orf72 protein and reduced protein levels in C9orf72 mutation carriers. Acta Neuropathol Commun. 2018;6:72 pubmed publisher
Li X, Eberhardt A, Hansen J, Bohmann D, Li H, Schor N. Methylation of the phosphatase-transcription activator EYA1 by protein arginine methyltransferase 1: mechanistic, functional, and structural studies. FASEB J. 2017;31:2327-2339 pubmed publisher
Meyer M, Benkusky N, Onal M, Pike J. Selective regulation of Mmp13 by 1,25(OH)2D3, PTH, and Osterix through distal enhancers. J Steroid Biochem Mol Biol. 2016;164:258-264 pubmed publisher
Holehonnur R, Lella S, Ho A, Luong J, Ploski J. The production of viral vectors designed to express large and difficult to express transgenes within neurons. Mol Brain. 2015;8:12 pubmed publisher

product information

Product Type :

Antibody

Product Name :

DYKDDDDK Tag Polyclonal Antibody, HRP

Catalog # :

PA1-984B-HRP

Quantity :

50 uL

Price :

US 394.00

Clonality :

Polyclonal

Purity :

Affinity chromatography

Host :

Rabbit

Reactivity :

Tag

Applications :

Western Blot: 1:500

Species :

Tag

Isotype :

IgG

Storage :

4 C, do not freeze

Description :

DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.

Immunogen :

Synthetic peptide corresponding to residues DYKDDDDK

Format :

Liquid

Applications w/Dilutions :

Western Blot: 1:500

Aliases :

DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag