6x-His Tag Polyclonal Antibody | Invitrogen PA1-983B product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

6x-His Tag Polyclonal Antibody

catalog :

PA1-983B

quantity :

200 ug

price :

US 390.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

application :

western blot, immunocytochemistry

citations: 21

Reference
Hu xdf mann M, Schulte D, Weischer S, Carlantoni C, Nakajima H, Mochizuki N, et al. Svep1 is a binding ligand of Tie1 and affects specific aspects of facial lymphatic development in a Vegfc-independent manner. elife. 2023;12: pubmed publisher
Moen J, Mohler K, Rogulina S, Shi X, Shen H, Rinehart J. Enhanced access to the human phosphoproteome with genetically encoded phosphothreonine. Nat Commun. 2022;13:7226 pubmed publisher
Jaroentomeechai T, Kwon Y, Liu Y, Young O, Bhawal R, Wilson J, et al. A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans. Nat Commun. 2022;13:6325 pubmed publisher
Parzych E, Du J, Ali A, Schultheis K, Frase D, Smith T, et al. DNA-delivered antibody cocktail exhibits improved pharmacokinetics and confers prophylactic protection against SARS-CoV-2. Nat Commun. 2022;13:5886 pubmed publisher
Konrath K, Liaw K, Wu Y, Zhu X, Walker S, Xu Z, et al. Nucleic acid delivery of immune-focused SARS-CoV-2 nanoparticles drives rapid and potent immunogenicity capable of single-dose protection. Cell Rep. 2022;38:110318 pubmed publisher
Inthong N, Kaewmongkol S, Meekhanon N, Suwan E, Sricharern W, Satchasataporn K, et al. Expression of recombinant 35 kDa fragment of VP2 protein of canine parvovirus using Escherichia coli expression system. Vet World. 2021;14:1682-1688 pubmed publisher
Van den Hoecke S, Ballegeer M, Vrancken B, Deng L, Job E, Roose K, et al. In Vivo Therapy with M2e-Specific IgG Selects for an Influenza A Virus Mutant with Delayed Matrix Protein 2 Expression. MBio. 2021;12:e0074521 pubmed publisher
Pietschmann J, Voepel N, Voß L, Rasche S, Schubert M, Kleines M, et al. Development of Fast and Portable Frequency Magnetic Mixing-Based Serological SARS-CoV-2-Specific Antibody Detection Assay. Front Microbiol. 2021;12:643275 pubmed publisher
Adams L, Dinnon K, Hou Y, Sheahan T, Heise M, Baric R. Critical ACE2 Determinants of SARS-CoV-2 and Group 2B Coronavirus Infection and Replication. MBio. 2021;12: pubmed publisher
Carlsson M, Kristiansson A, Bergwik J, Kanagarajan S, Bulow L, Akerstrom B, et al. Expression, Purification and Initial Characterization of Functional α1-Microglobulin (A1M) in Nicotiana benthamiana. Front Plant Sci. 2020;11:593773 pubmed publisher
Apóstolo N, Smukowski S, Vanderlinden J, Condomitti G, Rybakin V, Ten Bos J, et al. Synapse type-specific proteomic dissection identifies IgSF8 as a hippocampal CA3 microcircuit organizer. Nat Commun. 2020;11:5171 pubmed publisher
Walker S, Chokkalingam N, Reuschel E, Purwar M, Xu Z, Gary E, et al. SARS-CoV-2 Assays To Detect Functional Antibody Responses That Block ACE2 Recognition in Vaccinated Animals and Infected Patients. J Clin Microbiol. 2020;58: pubmed publisher
González de Cózar J, Carretero Junquera M, Ciesielski G, Miettinen S, Varjosalo M, Kaguni L, et al. A second hybrid-binding domain modulates the activity of Drosophila ribonuclease H1. J Biochem. 2020;: pubmed publisher
Ji C, Li Y, Kittredge A, Hopiavuori A, Ward N, Yao P, et al. Investigation and Restoration of BEST1 Activity in Patient-derived RPEs with Dominant Mutations. Sci Rep. 2019;9:19026 pubmed publisher
Bufe B, Teuchert Y, Schmid A, Pyrski M, Pérez Gómez A, Eisenbeis J, et al. Bacterial MgrB peptide activates chemoreceptor Fpr3 in mouse accessory olfactory system and drives avoidance behaviour. Nat Commun. 2019;10:4889 pubmed publisher
Chalenko Y, Kalinin E, Marchenkov V, Sysolyatina E, Surin A, Sobyanin K, et al. Phylogenetically Defined Isoforms of Listeria monocytogenes Invasion Factor InlB Differently Activate Intracellular Signaling Pathways and Interact with the Receptor gC1q-R. Int J Mol Sci. 2019;20: pubmed publisher
Deng L, Chang T, Wang Y, Li S, Wang S, Matsuyama S, et al. Heterosubtypic influenza protection elicited by double-layered polypeptide nanoparticles in mice. Proc Natl Acad Sci U S A. 2018;115:E7758-E7767 pubmed publisher
Barber K, Muir P, Szeligowski R, Rogulina S, Gerstein M, Sampson J, et al. Encoding human serine phosphopeptides in bacteria for proteome-wide identification of phosphorylation-dependent interactions. Nat Biotechnol. 2018;36:638-644 pubmed publisher
Deng L, Mohan T, Chang T, Gonzalez G, Wang Y, Kwon Y, et al. Double-layered protein nanoparticles induce broad protection against divergent influenza A viruses. Nat Commun. 2018;9:359 pubmed publisher
Oza J, Aerni H, Pirman N, Barber K, ter Haar C, Rogulina S, et al. Robust production of recombinant phosphoproteins using cell-free protein synthesis. Nat Commun. 2015;6:8168 pubmed publisher
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product information

Product Type :

Antibody

Product Name :

6x-His Tag Polyclonal Antibody

Catalog # :

PA1-983B

Quantity :

200 ug

Price :

US 390.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Tag

Applications :

Immunocytochemistry: 5 ug/mL, Western Blot: 1:250

Species :

Tag

Isotype :

IgG

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.

Immunogen :

Synthetic peptide corresponding to residues H H H H H H.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 5 ug/mL, Western Blot: 1:250

Aliases :

6His tag; 6X His; His Tag; Histidine Tag