DNMT3B Polyclonal Antibody | Invitrogen PA1-884 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

DNMT3B Polyclonal Antibody

catalog :

PA1-884

quantity :

100 µg

price :

US 446.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section, western blot knockout validation

more info or order :

Invitrogen product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; mouse; 1:1000; loading ...; fig 2f	Lopušná K, Nowialis P, Opavska J, Abraham A, Riva A, Opavsky R. Dnmt3b catalytic activity is critical for its tumour suppressor function in lymphomagenesis and is associated with c-Met oncogenic signalling. EBioMedicine. 2021;63:103191 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 1i	Nowialis P, Lopušná K, Opavska J, Haney S, Abraham A, Sheng P, et al. Catalytically inactive Dnmt3b rescues mouse embryonic development by accessory and repressive functions. Nat Commun. 2019;10:4374 pubmed publisher
western blot; mouse; fig 6	Termanis A, Torrea N, Culley J, Kerr A, Ramsahoye B, Stancheva I. The SNF2 family ATPase LSH promotes cell-autonomous de novo DNA methylation in somatic cells. Nucleic Acids Res. 2016;44:7592-604 pubmed publisher
western blot; human; fig 4	Zhang T, Termanis A, Ã–zkan B, Bao X, Culley J, de Lima Alves F, et al. G9a/GLP Complex Maintains Imprinted DNA Methylation in Embryonic Stem Cells. Cell Rep. 2016;15:77-85 pubmed publisher
	Pa xe7 o A, Leit xe3 o Castro J, Freitas R. Epigenetic Regulation of CDH1 Is Altered after HOXB7-Silencing in MDA-MB-468 Triple-Negative Breast Cancer Cells. Genes (Basel). 2021;12: pubmed publisher
	Lopušná K, Nowialis P, Opavska J, Abraham A, Riva A, Haney S, et al. Decreases in different Dnmt3b activities drive distinct development of hematologic malignancies in mice. J Biol Chem. 2021;296:100285 pubmed publisher
	Yan J, Tie G, Wang S, Tutto A, DeMarco N, Khair L, et al. Diabetes impairs wound healing by Dnmt1-dependent dysregulation of hematopoietic stem cells differentiation towards macrophages. Nat Commun. 2018;9:33 pubmed publisher

product information

Product Type :

Antibody

Product Name :

DNMT3B Polyclonal Antibody

Catalog # :

PA1-884

Quantity :

100 µg

Price :

US 446.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse

Applications :

Immunohistochemistry (Paraffin): 1:100-1:1,000, Western Blot: 2.0 µg/mL

Species :

Human, Mouse

Isotype :

IgG

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined.

Immunogen :

Synthetic peptide corresponding to the residues M(1) K G D S R H L N E E E G A(14) C of mouse DNMT3b

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 1:100-1:1,000, Western Blot: 2.0 µg/mL

Aliases :

DNA (cytosine-5-)-methyltransferase 3 beta; DNA (cytosine-5)-methyltransferase 3B; DNA methyltransferase 3 beta; DNA methyltransferase 3B; DNA methyltransferase HsaIIIB; DNA methyltransferase MmuIIIB; DNA MTase HsaIIIB; DNA MTase MmuIIIB; Dnmt3; DNMT3B; ICF; ICF1; M.HsaIIIB; m.MmuIIIB; MGC1090; MmuIIIB

more info or order :

Invitrogen product webpage