beta-Arrestin 1,2 Polyclonal Antibody | Invitrogen PA1-730 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

beta-Arrestin 1,2 Polyclonal Antibody

catalog :

PA1-730

quantity :

200 ug

price :

US 572.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 4

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:1000; loading ...; fig 1f	Weinberg Z, Zajac A, Phan T, Shiwarski D, Puthenveedu M. Sequence-Specific Regulation of Endocytic Lifetimes Modulates Arrestin-Mediated Signaling at the µ Opioid Receptor. Mol Pharmacol. 2017;91:416-427 pubmed publisher
immunocytochemistry; mouse; fig s1f	Pal K, Hwang S, Somatilaka B, Badgandi H, Jackson P, DeFea K, et al. Smoothened determines ?-arrestin-mediated removal of the G protein-coupled receptor Gpr161 from the primary cilium. J Cell Biol. 2016;212:861-75 pubmed publisher
	Chai F, Xu W, Musoke T, Tarabelsi G, Assaad S, Freedman J, et al. Structure-function analysis of β-arrestin Kurtz reveals a critical role of receptor interactions in downregulation of GPCR signaling in vivo. Dev Biol. 2019;: pubmed publisher
	Jiang K, Liu Y, Zhang J, Jia J. An intracellular activation of Smoothened that is independent of Hedgehog stimulation in Drosophila. J Cell Sci. 2018;131: pubmed publisher

product information

Product Type :

Antibody

Product Name :

beta-Arrestin 1,2 Polyclonal Antibody

Catalog # :

PA1-730

Quantity :

200 ug

Price :

US 572.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Rat

Applications :

Immunohistochemistry (Paraffin): 1:100-1:1,000, Immunoprecipitation: Assay-dependent, Western Blot: 3 ug/mL

Species :

Human, Rat

Isotype :

IgG

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

Beta Arrestin 1 is a member of a family of proteins widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold maolecule, beta Arrestin 1 is essential for mitogenic signalling and mediates agonist-dependent desensitization and internalization of Gprotein-coupled receptors (GPCRs, e.g., beta 2-adrenergic receptor). After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosporylated by G-protein receptor kinases (GRKs) on serine residues. Beta Arrestin 1 in the cytosol is phosphorylated by ERK1&2 on serine412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane. Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras, Raf, ERK1&2 signaling pathway.

Immunogen :

Synthetic Peptide: C D(384) D I V F E D F A R L R L K(397)

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Paraffin): 1:100-1:1,000, Immunoprecipitation: Assay-dependent, Western Blot: 3 ug/mL

Aliases :

ARB1; ARB2; ARR1; ARR2; Arrb1; Arrb2; arrestin 2; arrestin 3; arrestin beta 1; arrestin beta 2; arrestin beta-1; Arrestin beta-2; arrestin, beta 1; arrestin, beta 2; BARR2; BARRES; Beta-arrestin-1; beta-arrestin-2; Non-visual arrestin-2; Non-visual arrestin-3

more info or order :

Invitrogen product webpage