product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Glucocorticoid Receptor Polyclonal Antibody
catalog :
PA1-511A
quantity :
50 ug
price :
US 490.00
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry, immunocytochemistry, chromatin immunoprecipitation, immunohistochemistry - paraffin section, ChIP-Seq
more info or order :
citations: 48
Published Application/Species/Sample/DilutionReference
  • ChIP-Seq; mouse; loading ...; fig 1a, 7b
Lee H, Willi M, Shin H, Liu C, Hennighausen L. Progressing super-enhancer landscape during mammary differentiation controls tissue-specific gene regulation. Nucleic Acids Res. 2018;46:10796-10809 pubmed publisher
  • chromatin immunoprecipitation; human; loading ...; fig 4d
Lee B, Stallcup M. Glucocorticoid receptor binding to chromatin is selectively controlled by the coregulator Hic-5 and chromatin remodeling enzymes. J Biol Chem. 2017;292:9320-9334 pubmed publisher
  • ChIP-Seq; mouse; loading ...; fig 1
Lee H, Willi M, Wang C, Yang C, Smith H, Liu C, et al. Functional assessment of CTCF sites at cytokine-sensing mammary enhancers using CRISPR/Cas9 gene editing in mice. Nucleic Acids Res. 2017;45:4606-4618 pubmed publisher
  • ChIP-Seq; mouse; loading ...; fig s1a
Zeng X, Willi M, Shin H, Hennighausen L, Wang C. Lineage-Specific and Non-specific Cytokine-Sensing Genes Respond Differentially to the Master Regulator STAT5. Cell Rep. 2016;17:3333-3346 pubmed publisher
  • ChIP-Seq; mouse; loading ...; fig 2a
Willi M, Yoo K, Wang C, Trajanoski Z, Hennighausen L. Differential cytokine sensitivities of STAT5-dependent enhancers rely on Stat5 autoregulation. Nucleic Acids Res. 2016;44:10277-10291 pubmed
  • ChIP-Seq; mouse; loading ...; fig 5a
Shin H, Willi M, HyunYoo K, Zeng X, Wang C, Metser G, et al. Hierarchy within the mammary STAT5-driven Wap super-enhancer. Nat Genet. 2016;48:904-911 pubmed publisher
  • western blot; rat; 1:1000; fig 3
Kovacevic S, Nestorov J, Matić G, Elaković I. Fructose and stress induce opposite effects on lipid metabolism in the visceral adipose tissue of adult female rats through glucocorticoid action. Eur J Nutr. 2017;56:2115-2128 pubmed publisher
  • chromatin immunoprecipitation; mouse; fig 1d
Ma X, Xu L, Mueller E. Forkhead box A3 mediates glucocorticoid receptor function in adipose tissue. Proc Natl Acad Sci U S A. 2016;113:3377-82 pubmed publisher
  • western blot; rat; fig 3
Whitaker A, Farooq M, Edwards S, Gilpin N. Post-traumatic stress avoidance is attenuated by corticosterone and associated with brain levels of steroid receptor co-activator-1 in rats. Stress. 2016;19:69-77 pubmed publisher
Qiao X, Yang Y, Zhao Y, Wu X, Zhang L, Cai X, et al. Aurora Kinase A Regulates Cell Transitions in Glucocorticoid-Induced Bone Loss. Cells. 2023;12: pubmed publisher
Lee H, Willi M, Liu C, Hennighausen L. Cell-specific and shared regulatory elements control a multigene locus active in mammary and salivary glands. Nat Commun. 2023;14:4992 pubmed publisher
Angelousi A, Liapis G, Gazouli M, Kofotolios I, Sakellariou S, Boletis I, et al. Analysis of glucocorticoid receptor and microRNAs expression in pathological renal tissues. Mol Med Rep. 2023;28: pubmed publisher
Thorne A, Bansal A, Necker Brown A, Mostafa M, Gao A, Georgescu A, et al. Differential regulation of BIRC2 and BIRC3 expression by inflammatory cytokines and glucocorticoids in pulmonary epithelial cells. PLoS ONE. 2023;18:e0286783 pubmed publisher
Miller A, Daniels R, Sheng J, Wu T, Handa R. Glucocorticoid regulation of diurnal spine plasticity in the murine ventromedial prefrontal cortex. J Neuroendocrinol. 2022;34:e13212 pubmed publisher
Kim U, Kim S, Kim N, Shin H. Mammary-Enriched Transcription Factors Synergize to Activate the Wap Super-Enhancer for Mammary Gland Development. Int J Mol Sci. 2022;23: pubmed publisher
Patel T, Hammelman J, Aziz S, Jang S, Closser M, Michaels T, et al. Transcriptional dynamics of murine motor neuron maturation in vivo and in vitro. Nat Commun. 2022;13:5427 pubmed publisher
Luo J, Li J, Li T, Zhang Z, Chen G, Li Q, et al. PIAS1 Alleviates Hepatic Ischemia-Reperfusion Injury in Mice through a Mechanism Involving NFATc1 SUMOylation. Dis Markers. 2022;2022:4988539 pubmed publisher
Kim J, Lei Y, Lu X, Kim C. Glucocorticoid-glucocorticoid receptor-HCN1 channels reduce neuronal excitability in dorsal hippocampal CA1 neurons. Mol Psychiatry. 2022;27:4035-4049 pubmed publisher
Shao S, Wang Y, Zhao Y, Xu Y, Wang T, Du K, et al. Identification of multiple isoforms of glucocorticoid receptor in nasal polyps of patients with chronic rhinosinusitis. J Otolaryngol Head Neck Surg. 2022;51:25 pubmed publisher
Ehlers L, Kirchner M, Mertins P, Strehl C, Buttgereit F, Gaber T. The Anti-Glucocorticoid Receptor Antibody Clone 5E4: Raising Awareness of Unspecific Antibody Binding. Int J Mol Sci. 2022;23: pubmed publisher
Bansal A, Mostafa M, Kooi C, Sasse S, Michi A, Shah S, et al. Interplay between nuclear factor-κB, p38 MAPK, and glucocorticoid receptor signaling synergistically induces functional TLR2 in lung epithelial cells. J Biol Chem. 2022;298:101747 pubmed publisher
Yokosuka T, Ito M, Yoshino Y, Hirose A, Nakamura W, Sakurai Y, et al. Using the in vitro drug sensitivity test to identify candidate treatments for transient abnormal myelopoiesis. Br J Haematol. 2022;196:764-768 pubmed publisher
Præstholm S, Correia C, Goitea V, Siersbæk M, Jørgensen M, Havelund J, et al. Impaired glucocorticoid receptor expression in liver disrupts feeding-induced gene expression, glucose uptake, and glycogen storage. Cell Rep. 2021;37:109938 pubmed publisher
Ma P, Zhang Y, Liang Q, Yin Y, Wang S, Han R, et al. Mifepristone (RU486) inhibits dietary lipid digestion by antagonizing the role of glucocorticoid receptor on lipase transcription. iScience. 2021;24:102507 pubmed publisher
Torres Velarde J, Kolora S, Khudyakov J, Crocker D, Sudmant P, V xe1 zquez Medina J. Elephant seal muscle cells adapt to sustained glucocorticoid exposure by shifting their metabolic phenotype. Am J Physiol Regul Integr Comp Physiol. 2021;321:R413-R428 pubmed publisher
Stott R, Kritsky O, Tsai L. Profiling DNA break sites and transcriptional changes in response to contextual fear learning. PLoS ONE. 2021;16:e0249691 pubmed publisher
Bakour N, Moriarty F, Moore G, Robson T, Annett S. Prognostic Significance of Glucocorticoid Receptor Expression in Cancer: A Systematic Review and Meta-Analysis. Cancers (Basel). 2021;13: pubmed publisher
Lee H, Willi M, Kuhns T, Liu C, Hennighausen L. Redundant and non-redundant cytokine-activated enhancers control Csn1s2b expression in the lactating mouse mammary gland. Nat Commun. 2021;12:2239 pubmed publisher
Johnson T, Paakinaho V, Kim S, Hager G, Presman D. Genome-wide binding potential and regulatory activity of the glucocorticoid receptor's monomeric and dimeric forms. Nat Commun. 2021;12:1987 pubmed publisher
Elakovi x107 I, Kova x10d evi x107 S, Vojnovi x107 Milutinovi x107 D, Nikoli x107 Koki x107 A, Glban A, Spasi x107 M, et al. Fructose Consumption Affects Glucocorticoid Signaling in the Liver of Young Female Rats. Nutrients. 2020;12: pubmed publisher
Mostafa M, Bansal A, Michi A, Sasse S, Proud D, Gerber A, et al. Genomic determinants implicated in the glucocorticoid-mediated induction of KLF9 in pulmonary epithelial cells. J Biol Chem. 2021;296:100065 pubmed publisher
Wilflingseder J, Willi M, Lee H, Olauson H, Jankowski J, Ichimura T, et al. Enhancer and super-enhancer dynamics in repair after ischemic acute kidney injury. Nat Commun. 2020;11:3383 pubmed publisher
Hong J, Lim J, Carvalho F, Cho J, Vaidyanathan B, Yu S, et al. Long-Term Programming of CD8 T Cell Immunity by Perinatal Exposure to Glucocorticoids. Cell. 2020;180:847-861.e15 pubmed publisher
Heck A, Thompson M, Uht R, Handa R. Sex-Dependent Mechanisms of Glucocorticoid Regulation of the Mouse Hypothalamic Corticotropin-Releasing Hormone Gene. Endocrinology. 2020;161: pubmed publisher
Scheimann J, Moloney R, Mahbod P, Morano R, Fitzgerald M, Hoskins O, et al. Conditional deletion of glucocorticoid receptors in rat brain results in sex-specific deficits in fear and coping behaviors. elife. 2019;8: pubmed publisher
Kalvisa A, Siersbæk M, Præstholm S, Christensen L, Nielsen R, Stöhr O, et al. Insulin signaling and reduced glucocorticoid receptor activity attenuate postprandial gene expression in liver. PLoS Biol. 2018;16:e2006249 pubmed publisher
Zubeldia Plazaola A, Recalde Percaz L, Moragas N, Alcaraz M, Chen X, Mancino M, et al. Glucocorticoids promote transition of ductal carcinoma in situ to invasive ductal carcinoma by inducing myoepithelial cell apoptosis. Breast Cancer Res. 2018;20:65 pubmed publisher
Rider C, Altonsy M, Mostafa M, Shah S, Sasse S, Manson M, et al. Long-Acting β2-Adrenoceptor Agonists Enhance Glucocorticoid Receptor (GR)-Mediated Transcription by Gene-Specific Mechanisms Rather Than Generic Effects via GR. Mol Pharmacol. 2018;94:1031-1046 pubmed publisher
Lee B, Stallcup M. Different chromatin and DNA sequence characteristics define glucocorticoid receptor binding sites that are blocked or not blocked by coregulator Hic-5. PLoS ONE. 2018;13:e0196965 pubmed publisher
Sacta M, Tharmalingam B, Coppo M, Rollins D, Deochand D, Benjamin B, et al. Gene-specific mechanisms direct glucocorticoid-receptor-driven repression of inflammatory response genes in macrophages. elife. 2018;7: pubmed publisher
Enuka Y, Feldman M, Chowdhury A, Srivastava S, Lindzen M, Sas Chen A, et al. Epigenetic mechanisms underlie the crosstalk between growth factors and a steroid hormone. Nucleic Acids Res. 2017;: pubmed publisher
Lerch J, Alexander J, Madalena K, Motti D, Quach T, Dhamija A, et al. Stress Increases Peripheral Axon Growth and Regeneration through Glucocorticoid Receptor-Dependent Transcriptional Programs. Eneuro. 2017;4: pubmed publisher
Pooley J, Flynn B, Grøntved L, Baek S, Guertin M, Kershaw Y, et al. Genome-Wide Identification of Basic Helix-Loop-Helix and NF-1 Motifs Underlying GR Binding Sites in Male Rat Hippocampus. Endocrinology. 2017;158:1486-1501 pubmed publisher
Arango Lievano M, Lambert W, Bath K, Garabedian M, Chao M, Jeanneteau F. Neurotrophic-priming of glucocorticoid receptor signaling is essential for neuronal plasticity to stress and antidepressant treatment. Proc Natl Acad Sci U S A. 2015;112:15737-42 pubmed publisher
Gallina D, Zelinka C, Cebulla C, Fischer A. Activation of glucocorticoid receptors in Müller glia is protective to retinal neurons and suppresses microglial reactivity. Exp Neurol. 2015;273:114-25 pubmed publisher
Lim H, Uhlenhaut N, Rauch A, Weiner J, Hübner S, Hübner N, et al. Genomic redistribution of GR monomers and dimers mediates transcriptional response to exogenous glucocorticoid in vivo. Genome Res. 2015;25:836-44 pubmed publisher
Stavreva D, Coulon A, Baek S, Sung M, John S, Stixova L, et al. Dynamics of chromatin accessibility and long-range interactions in response to glucocorticoid pulsing. Genome Res. 2015;25:845-57 pubmed publisher
Macut D, Božić Antić I, Nestorov J, Topalović V, Bjekić Macut J, Panidis D, et al. The influence of combined oral contraceptives containing drospirenone on hypothalamic-pituitary-adrenocortical axis activity and glucocorticoid receptor expression and function in women with polycystic ovary syndrome. Hormones (Athens). 2015;14:109-17 pubmed publisher
product information
Product Type :
Antibody
Product Name :
Glucocorticoid Receptor Polyclonal Antibody
Catalog # :
PA1-511A
Quantity :
50 ug
Price :
US 490.00
Clonality :
Polyclonal
Purity :
Antigen affinity chromatography
Host :
Rabbit
Reactivity :
Human, Mouse, Rat
Applications :
Immunocytochemistry: 1 ug/mL, Immunohistochemistry (Paraffin): 1:200, Western Blot: 5 ug/mL
Species :
Human, Mouse, Rat
Isotype :
IgG
Storage :
-20 C, Avoid Freeze/Thaw Cycles
Description :
Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity
Immunogen :
Synthetic peptide corresponding to residues D(346) Q K P I F N V I P P I P V G S E N W N R C(367) from human GR.
Format :
Lyophilized
Applications w/Dilutions :
Immunocytochemistry: 1 ug/mL, Immunohistochemistry (Paraffin): 1:200, Western Blot: 5 ug/mL
Aliases :
cytosolic/nuclear receptor; fb13f09; GCCR; Gcr; GCRST; glucocorticoid nuclear receptor variant 1; glucocorticoid receptor; glucocorticoid receptor 1; glucocorticoid receptor alpha; GR; gr alpha; GR Kit; Grl; Grl1; Grl-1; Nr3c1; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); OTTHUMP00000222854; OTTHUMP00000222858; utouto; utut; wu:fb13f09; zgc:113038
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA