TPH1 Polyclonal Antibody | Invitrogen PA1-4678 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

TPH1 Polyclonal Antibody

catalog :

PA1-4678

quantity :

100 uL

price :

US 474.00

clonality :

polyclonal

host :

domestic sheep

conjugate :

nonconjugated

reactivity :

human, rat, domestic rabbit

application :

western blot, immunohistochemistry, immunohistochemistry - frozen section

product information

Product Type :

Antibody

Product Name :

TPH1 Polyclonal Antibody

Catalog # :

PA1-4678

Quantity :

100 uL

Price :

US 474.00

Clonality :

Polyclonal

Purity :

Affinity chromatography

Host :

Sheep

Reactivity :

Human, Rabbit, Rat

Applications :

Immunohistochemistry (Frozen): 1:1,000, Immunohistochemistry: 1:1,000, Western Blot: 1:1,000

Species :

Human, Rabbit, Rat

Isotype :

IgG

Storage :

-20 C, Avoid Freeze/Thaw Cycles

Description :

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAM K II) (Jiang et al., 2000; Johansen et al., 1996). Both PKA and CAM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).

Immunogen :

Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Frozen): 1:1,000, Immunohistochemistry: 1:1,000, Western Blot: 1:1,000

Aliases :

indoleacetic acid-5-hydroxylase; L-tryptophan hydroxylase; MGC119994; TPH; Tph1; TPH2; TPRH; TRPH; tryptophan 5-hydroxylase 1; Tryptophan 5-monooxygenase 1; tryptophan hydroxylase; tryptophan hydroxylase (tryptophan 5-monooxygenase); tryptophan hydroxylase 1; tryptophan hydroxylase 1 (tryptophan 5-monooxygenase)