NPM1 (mutant) Polyclonal Antibody | Invitrogen PA1-46356 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

NPM1 (mutant) Polyclonal Antibody

catalog :

PA1-46356

quantity :

100 uL

price :

US 454.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section

citations: 12

Reference
Kaseb H, Visconte V, Socha D, Crane G, Durkin L, Cook J, et al. The clinicopathologic significance of NPM1 mutation and ability to detect mutated NPM1 by immunohistochemistry in non-AML myeloid neoplasms. Genes Chromosomes Cancer. 2023;62:573-580 pubmed publisher
Huang J, Sun M, Tao Y, Ren J, Peng M, Jing Y, et al. Cytoplasmic Expression of TP53INP2 Modulated by Demethylase FTO and Mutant NPM1 Promotes Autophagy in Leukemia Cells. Int J Mol Sci. 2023;24: pubmed publisher
Skayneh H, Jishi B, Hleihel R, Hamie M, El Hajj R, Deleuze Masquefa C, et al. EAPB0503, an Imidazoquinoxaline Derivative Modulates SENP3/ARF Mediated SUMOylation, and Induces NPM1c Degradation in NPM1 Mutant AML. Int J Mol Sci. 2022;23: pubmed publisher
Xiao Q, Lei L, Ren J, Peng M, Jing Y, Jiang X, et al. Mutant NPM1-Regulated FTO-Mediated m6A Demethylation Promotes Leukemic Cell Survival via PDGFRB/ERK Signaling Axis. Front Oncol. 2022;12:817584 pubmed publisher
Aggarwal N, Alston E, Pinkus G, Weinberg O. Role of immunohistochemistry and flow cytometry in minimal residual disease detection in NPM1-mutated AML. Int J Lab Hematol. 2022;44:e76-e78 pubmed publisher
Djamai H, Berrou J, Dupont M, Coud xe9 M, Delord M, Clappier E, et al. Biological Effects of BET Inhibition by OTX015 (MK-8628) and JQ1 in NPM1-Mutated (NPM1c) Acute Myeloid Leukemia (AML). Biomedicines. 2021;9: pubmed publisher
Jing Y, Jiang X, Lei L, Peng M, Ren J, Xiao Q, et al. Mutant NPM1-regulated lncRNA HOTAIRM1 promotes leukemia cell autophagy and proliferation by targeting EGR1 and ULK3. J Exp Clin Cancer Res. 2021;40:312 pubmed publisher
Lopez A, Patel S, Geyer J, Racchumi J, Chadburn A, Simonson P, et al. Comparison of Multiple Clinical Testing Modalities for Assessment of NPM1-Mutant AML. Front Oncol. 2021;11:701318 pubmed publisher
. Abstracts from USCAP 2021: Hematopathology (664-756). Mod Pathol. 2021;34:927-1049 pubmed publisher
Gadewal N, Kumar R, Aher S, Gardane A, Gaur T, Varma A, et al. miRNA-mRNA Profiling Reveals Prognostic Impact of SMC1A Expression in Acute Myeloid Leukemia. Oncol Res. 2020;28:321-330 pubmed publisher
Patel S, Lipschitz M, Pinkus G, Weirather J, Pozdnyakova O, Mason E, et al. Multiparametric in situ imaging of NPM1-mutated acute myeloid leukemia reveals prognostically-relevant features of the marrow microenvironment. Mod Pathol. 2020;33:1380-1388 pubmed publisher
Yang L, Wang L, Yang Z, Jin H, Zou Q, Zhan Q, et al. Up-regulation of EMT-related gene VCAN by NPM1 mutant-driven TGF-β/cPML signalling promotes leukemia cell invasion. J Cancer. 2019;10:6570-6583 pubmed publisher

product information

Product Type :

Antibody

Product Name :

NPM1 (mutant) Polyclonal Antibody

Catalog # :

PA1-46356

Quantity :

100 uL

Price :

US 454.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse

Applications :

Flow Cytometry: 1:100, Immunocytochemistry: 1:50-1:100, Immunohistochemistry (Paraffin): 1:50-1:100, Immunoprecipitation: 1:50, Western Blot: 1:500

Species :

Human, Mouse

Isotype :

IgG

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

NPM1 (Nucleophosmin 1, B23, nutramin, NO38) is a ubiquitously expressed phosphoprotein involved in ribosome assembly/transport, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication, and regulation of p53. NPM1 continuously shuttles between the nucleus, cytoplasm, nucleolus and chaperoning core histones from the nucleus to the cytoplasm. NPM1 has been shown to bind nucleic acid, prevent protein aggregation via its chaperon activities, protect enzymes during thermal denaturation, and facilitate renaturation of chemically denatured proteins. In its cellular structure role, there is evidence suggesting NPM1 is associated with the centrosome, and is the substrate of CDK2/cyclin E during duplication of centrosomes (cellular division). Due to the NPM1 gene interaction with several tumor-associated chromosome translocations, NPM1 is thought to be a portion of several fusion proteins: NPM-ALK, NPM-RAR, and NPM-MLF1. While it is not thought to be part of the transforming potential of these fusion proteins, NPM1 is believed to act as the interface for oligomerization and oncogenic conversion of these tumor promoting fusion proteins. Further, NPM1 is also known to sequester the tumor suppressor RF in the nucleolus, protecting it from degradation until it is necessary. Dysfunction of the NPM1 protein is associated with diseases such as acute myeloid leukemia and lymphomatoid papulosis.

Immunogen :

Synthetic peptide corresponding to a C-terminal region (within residues 250-294) of human mutant nucleophosmin.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: 1:100, Immunocytochemistry: 1:50-1:100, Immunohistochemistry (Paraffin): 1:50-1:100, Immunoprecipitation: 1:50, Western Blot: 1:500

Aliases :

B23; B23 antibody; MGC104254; mNPM antibody; mNPM1 antibody; NO38 antibody; NPM; NPM1; Nucleolar phosphoprotein B23; Nucleolar protein NO38; nucleophosmin; nucleophosmin (nucleolar phosphoprotein B23, numatrin); nucleophosmin 1; nucleophosmin/nucleoplasmin family, member 1; Numatrin; numatrin antibody; testicular tissue protein Li 128