Histone H2A.X Polyclonal Antibody | Invitrogen PA1-41004 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Histone H2A.X Polyclonal Antibody

catalog :

PA1-41004

quantity :

100 ug

price :

US 514.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, flow cytometry, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; human; fig 1	Winick Ng W, Caetano F, Winick Ng J, Morey T, Heit B, Rylett R. 82-kDa choline acetyltransferase and SATB1 localize to ?-amyloid induced matrix attachment regions. Sci Rep. 2016;6:23914 pubmed publisher
	Ku J, Marsh S, Nai M, Robinson K, Teo D, Zhong F, et al. Skin models for cutaneous melioidosis reveal Burkholderia infection dynamics at wound's edge with inflammasome activation, keratinocyte extrusion and epidermal detachment. Emerg Microbes Infect. 2021;10:2326-2339 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Histone H2A.X Polyclonal Antibody

Catalog # :

PA1-41004

Quantity :

100 ug

Price :

US 514.00

Clonality :

Polyclonal

Purity :

Protein G

Host :

Rabbit

Reactivity :

Human, Mouse, Ovine

Applications :

ChIP Assay: 1.5 ug per million cells, ELISA: 1:2,000, Flow Cytometry: Assay-Dependent, Immunohistochemistry (Paraffin): 5 ug/mL, Western Blot: 2 ug/mL

Species :

Human, Mouse, Ovine

Isotype :

IgG

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle. In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX. The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs. The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner. Structurally, H2A.x contains 143 amino acid residues. Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs. There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA. The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA. Histone H2A.X maps to the 11q23.2-q23.3 region of the human chromosome. Histone H2A.x contributes to histone-formation and therefore the structure of DNA. Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.

Immunogen :

Mixture of synthetic peptides containing residues 1-15 and 81-96 of Human Histone H2A

Format :

Liquid

Applications w/Dilutions :

ChIP Assay: 1.5 ug per million cells, ELISA: 1:2,000, Flow Cytometry: Assay-Dependent, Immunohistochemistry (Paraffin): 5 ug/mL, Western Blot: 2 ug/mL

Aliases :

AW228881; gamma H2AX; gammaH2ax; H2A histone family member X; H2A histone family, member X; H2A.X; H2A.X variant histone; H2A/X; h2afx; H2ax; H2AX histone; Hist5-2ax; histone 5 protein 2ax; histone H2A.x; Histone H2a/x; Histone H2AX; RGD1566119; similar to H2A histone family, member X; zgc:56329

more info or order :

Invitrogen product webpage