5-Methylcytosine Polyclonal Antibody | Invitrogen PA1-30675 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

5-Methylcytosine Polyclonal Antibody

catalog :

PA1-30675

quantity :

250 ug

price :

US 612.00

clonality :

polyclonal

host :

domestic sheep

conjugate :

nonconjugated

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - frozen section, dot blot

citations: 1

Reference
Zhou J, Tang J, Sun W, Wang H. PGK1 facilities cisplatin chemoresistance by triggering HSP90/ERK pathway mediated DNA repair and methylation in endometrial endometrioid adenocarcinoma. Mol Med. 2019;25:11 pubmed publisher

product information

Product Type :

Antibody

Product Name :

5-Methylcytosine Polyclonal Antibody

Catalog # :

PA1-30675

Quantity :

250 ug

Price :

US 612.00

Clonality :

Polyclonal

Purity :

Protein G

Host :

Sheep

Reactivity :

Chemical

Applications :

Dot Blot: Assay-dependent, Flow Cytometry: Assay-dependent, Immunocytochemistry: 5 ug/mL, Immunohistochemistry (Frozen): 10 ug/mL, Immunoprecipitation: 25-100 ug/mL, Western Blot: 1:1,000

Species :

Chemical

Isotype :

IgG

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

Methylation of DNA cytosine bases plays important roles in the regulation of gene transcription. It has been estimated that 60 to 90% of the cytosines in CpG dinucleotides are methylated. An increase in methyl-CpG correlates with transcriptional silencing for the whole chromosome especially in developmentally regulated genes. Methylation is believed to lead to transcriptional silencing by multiple mechanisms including alteration of transcription factor binding to promoters and alteration in chromatin structure.

Immunogen :

5-methyl cytosine molecule conjugated to KLH.

Format :

Liquid

Applications w/Dilutions :

Dot Blot: Assay-dependent, Flow Cytometry: Assay-dependent, Immunocytochemistry: 5 ug/mL, Immunohistochemistry (Frozen): 10 ug/mL, Immunoprecipitation: 25-100 ug/mL, Western Blot: 1:1,000

Aliases :

5mc; 5-mC; 5-meC; 5-Methyl-cytosine; C5H7NO3