Caspase 8 Polyclonal Antibody | Invitrogen PA1-29159 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Caspase 8 Polyclonal Antibody

catalog :

PA1-29159

quantity :

500 uL

price :

US 514.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; 1:25; fig 2c western blot; human; loading ...; fig 1b	Pu X, Storr S, Zhang Y, Rakha E, Green A, Ellis I, et al. Caspase-3 and caspase-8 expression in breast cancer: caspase-3 is associated with survival. Apoptosis. 2017;22:357-368 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 4c	Martin B, Wang C, Zhang C, Kang Z, Gulen M, Zepp J, et al. T cell-intrinsic ASC critically promotes T(H)17-mediated experimental autoimmune encephalomyelitis. Nat Immunol. 2016;17:583-92 pubmed publisher
	Jagadeesh N, Belur S, Campbell B, Inamdar S. The fucose-specific lectin ANL from Aspergillus niger possesses anti-cancer activity by inducing the intrinsic apoptosis pathway in hepatocellular and colon cancer cells. Cell Biochem Funct. 2021;39:401-412 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Caspase 8 Polyclonal Antibody

Catalog # :

PA1-29159

Quantity :

500 uL

Price :

US 514.00

Clonality :

Polyclonal

Purity :

protein A

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Applications :

Immunocytochemistry: 1:50-1:200, Immunohistochemistry (Paraffin): 1:1,000, Immunoprecipitation: 1:2,000, Western Blot: 1:500

Species :

Human, Mouse, Rat

Isotype :

IgG

Storage :

4 C, do not freeze

Description :

Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases play a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a pro-domain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. Caspase 8 is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of Caspase 8 suggests that it may interact with Fas-interacting protein FADD. Caspase 8 was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Caspase 8 binds to the death effector domain (DED) of FADD through an analogous DED domain present in tandem in the pro-form of the Caspase 8 protein. Activated Caspase 8 then activates other downstream caspases including Caspase 9, thereby committing the cell to undergo apoptosis. In addition, Caspase 8 also reacts with Jurkat cells and Tonsil. Overexpression of Caspase 8 induces apoptosis, which can be blocked by inhibitors specific for the ICE family. Many alternatively spliced transcript variants encoding different isoforms have been described for Caspase 8, however, not all variants have had their full-length sequences determined.

Immunogen :

The epitope corresponding to amino acids 217-350 mapping within the caspase-8 p18 subunit of human origin

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:50-1:200, Immunohistochemistry (Paraffin): 1:1,000, Immunoprecipitation: 1:2,000, Western Blot: 1:500

Aliases :

ALPS2B; apoptosis-related cysteine peptidase; apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; CASP8; CASP-8; caspase 8; caspase 8, apoptosis-related cysteine peptidase; caspase 8, apoptosis-related cysteine protease; Caspase8; caspase-8; Caspase-8 precursor; Caspase-8 subunit p10; Caspase-8 subunit p18; EC 3.4.22.61; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; Fas-linked ICE-like protease; FLICE; FLJ17672; ICE8; ICE-like apoptotic protease 5; MACH; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; MCH5; MGC78473; MORT1-associated ced-3 homolog; OTTHUMP00000163720; OTTHUMP00000165063; OTTHUMP00000206557; OTTHUMP00000206581

more info or order :

Invitrogen product webpage