Phospho-c-Raf (Ser621) Polyclonal Antibody | Invitrogen PA1-26655 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-c-Raf (Ser621) Polyclonal Antibody

catalog :

PA1-26655

quantity :

50µL

price :

US 349.00

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, chromatin immunoprecipitation

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:5000; loading ...; fig 4c	Mitra S, Ghosh B, Gayen N, Roy J, Mandal A. Bipartite Role of Heat Shock Protein 90 (Hsp90) Keeps CRAF Kinase Poised for Activation. J Biol Chem. 2016;291:24579-24593 pubmed
chromatin immunoprecipitation; human; 3 ug immunoprecipitation; human; 3 ug western blot; human	Geil W, Yen A. Nuclear Raf-1 kinase regulates the CXCR5 promoter by associating with NFATc3 to drive retinoic acid-induced leukemic cell differentiation. FEBS J. 2014;281:1170-80 pubmed publisher
western blot; human	Jensen H, Styskal L, Tasseff R, Bunaciu R, Congleton J, Varner J, et al. The Src-family kinase inhibitor PP2 rescues inducible differentiation events in emergent retinoic acid-resistant myeloblastic leukemia cells. PLoS ONE. 2013;8:e58621 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Phospho-c-Raf (Ser621) Polyclonal Antibody

Catalog # :

PA1-26655

Quantity :

50µL

Price :

US 349.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Applications :

Western Blot: 0.1-1 µg/ml

Species :

Human, Mouse, Rat

Isotype :

IgG

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Immunogen :

Synthetic phosphopeptide corresponding to a portion of human c-Raf containing serine 621. The sequence is conserved in mouse and rat.

Format :

Liquid

Applications w/Dilutions :

Western Blot: 0.1-1 µg/ml

Aliases :

CRAF, NS5, Raf-1, c-Raf

PubMed References :

23554907, 24330068