6x-His Tag Polyclonal Antibody, HRP | Invitrogen PA1-23024 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

6x-His Tag Polyclonal Antibody, HRP

catalog :

PA1-23024

quantity :

100 ug

price :

US 674.00

clonality :

polyclonal

host :

domestic goat

conjugate :

HRP

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry

citations: 5

Reference
Mulinti P, Diekj xfc rgen D, Kurtzeborn K, Balasubramanian N, Stafslien S, Grainger D, et al. Anti-Coagulant and Antimicrobial Recombinant Heparin-Binding Major Ampullate Spidroin 2 (MaSp2) Silk Protein. Bioengineering (Basel). 2022;9: pubmed publisher
Klimka A, Mertins S, Nicolai A, Rummler L, Higgins P, Günther S, et al. Epitope-specific immunity against Staphylococcus aureus coproporphyrinogen III oxidase. NPJ Vaccines. 2021;6:11 pubmed publisher
Wang Y, Garrido Oter R, Wu J, Winkelmüller T, Agler M, Colby T, et al. Site-specific cleavage of bacterial MucD by secreted proteases mediates antibacterial resistance in Arabidopsis. Nat Commun. 2019;10:2853 pubmed publisher
Mitchell L, Yagiz K, Hofacre A, Viaud S, Munday A, Espinoza F, et al. PD-L1 checkpoint blockade delivered by retroviral replicating vector confers anti-tumor efficacy in murine tumor models. Oncotarget. 2019;10:2252-2269 pubmed publisher
Gardner B, Castanzo D, Chowdhury S, Stjepanovic G, Stefely M, Hurley J, et al. The peroxisomal AAA-ATPase Pex1/Pex6 unfolds substrates by processive threading. Nat Commun. 2018;9:135 pubmed publisher

product information

Product Type :

Antibody

Product Name :

6x-His Tag Polyclonal Antibody, HRP

Catalog # :

PA1-23024

Quantity :

100 ug

Price :

US 674.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Goat

Reactivity :

Tag

Applications :

ELISA: 1:10,000-1:100,000, Immunocytochemistry: 1:200-1:500, Immunohistochemistry: Assay-dependent, Western Blot: 1:1,000-1:5,000

Species :

Tag

Isotype :

IgG

Storage :

4 C, do not freeze

Description :

The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.

Immunogen :

6-His (HHHHHH) conjugated to KLH

Format :

Liquid

Applications w/Dilutions :

ELISA: 1:10,000-1:100,000, Immunocytochemistry: 1:200-1:500, Immunohistochemistry: Assay-dependent, Western Blot: 1:1,000-1:5,000

Aliases :

6His tag; 6X His; His Tag; Histidine Tag