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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
LC3A/LC3B Polyclonal Antibody
catalog :
PA1-16931
quantity :
100 uL
price :
US 496.00
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat, dogs, zebrafish
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
Invitrogen product webpage
citations: 23
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 4a
Zou P, Liu L, Zheng L, Payne K, Manjili M, Idowu M, et al. Coordinated Upregulation of Mitochondrial Biogenesis and Autophagy in Breast Cancer Cells: The Role of Dynamin Related Protein-1 and Implication for Breast Cancer Treatment. Oxid Med Cell Longev. 2016;2016:4085727 pubmed
  • western blot; rat; fig 8
Shruthi K, Reddy S, Reddy P, Shivalingam P, Harishankar N, Reddy G. Amelioration of neuronal cell death in a spontaneous obese rat model by dietary restriction through modulation of ubiquitin proteasome system. J Nutr Biochem. 2016;33:73-81 pubmed publisher
  • western blot; human; loading ...; fig s2a
Pereira D, Simões A, Gomes S, Castro R, Carvalho T, Rodrigues C, et al. MEK5/ERK5 signaling inhibition increases colon cancer cell sensitivity to 5-fluorouracil through a p53-dependent mechanism. Oncotarget. 2016;7:34322-40 pubmed publisher
So x142 ek P, Czechowska E, Sowa Ku x107 ma M, Stachowicz K, Kaczka P, Tab x119 cka x141 onczy x144 ska A. Elucidating the molecular mechanisms underlying the induction of autophagy by antidepressant-like substances in C57BL/6J mouse testis model upon LPS challenge. Cell Commun Signal. 2023;21:251 pubmed publisher
Wasfey E, Shaaban M, Essam M, Ayman Y, Kamar S, Mohasseb T, et al. Infliximab Ameliorates Methotrexate-Induced Nephrotoxicity in Experimental Rat Model: Impact on Oxidative Stress, Mitochondrial Biogenesis, Apoptotic and Autophagic Machineries. Cell Biochem Biophys. 2023;81:717-726 pubmed publisher
Rutledge C, Lagranha C, Chiba T, Redding K, Stolz D, Goetzman E, et al. Metformin preconditioning protects against myocardial stunning and preserves protein translation in a mouse model of cardiac arrest. J Mol Cell Cardiol Plus. 2023;4: pubmed publisher
Lai Y, Chang C, Chen Y, Nguyen T, Xu J, Chen Y, et al. Optogenetically engineered Ca2+ oscillation-mediated DRP1 activation promotes mitochondrial fission and cell death. J Cell Sci. 2023;136: pubmed publisher
Thangavel H, Lizardo K, Dhanyalayam D, de Assis S, Nagajyothi J. Diets Differently Regulate Tumorigenesis in Young E0771 Syngeneic Breast Cancer Mouse Model. J Clin Med. 2023;12: pubmed publisher
Ma Q, Zhou J, Yang Z, Xue Y, Xie X, Li T, et al. Mingmu Xiaoyao granules regulate the PI3K/Akt/mTOR signaling pathway to reduce anxiety and depression and reverse retinal abnormalities in rats. Front Pharmacol. 2022;13:1003614 pubmed publisher
Balkrishna A, Mulay V, Verma S, Srivastava J, Lochab S, Varshney A. Penta-O-Galloyl-β-D-Glucose in Pistacia integerrima Targets AMPK-ULK1 and ERK/STAT3 Signaling Axes to Induce ROS-Independent Autophagic Cell Death in Human Lung Cancer Cells. Front Pharmacol. 2022;13:889335 pubmed publisher
Liu X, Balaraman K, Lynch C, Hebron M, Shah P, Hu S, et al. Inhibition of Ubiquitin-Specific Protease-13 Improves Behavioral Performance in Alpha-Synuclein Expressing Mice. Int J Mol Sci. 2022;23: pubmed publisher
Martins Marques T, Costa M, Catarino S, Simões I, Aasen T, Enguita F, et al. Cx43-mediated sorting of miRNAs into extracellular vesicles. EMBO Rep. 2022;23:e54312 pubmed publisher
Leung Z, Abu Rafea B, Watson A, Betts D. Free fatty acid treatment of mouse preimplantation embryos demonstrates contrasting effects of palmitic acid and oleic acid on autophagy. Am J Physiol Cell Physiol. 2022;322:C833-C848 pubmed publisher
Liu Y, Wang Y, Yao D, Chen X, Zhang F, Feng Y, et al. Diane-35 and Metformin Induce Autophagy and Apoptosis in Polycystic Ovary Syndrome Women with Early-Stage Endometrial Carcinoma. Genes (Basel). 2022;13: pubmed publisher
Ikeh K, Lamkin E, Crompton A, Deutsch J, Fisher K, Gray M, et al. REV1 Inhibition Enhances Radioresistance and Autophagy. Cancers (Basel). 2021;13: pubmed publisher
Wang S, Qian H, Zhang L, Liu P, Zhuang D, Zhang Q, et al. Inhibition of Calcineurin/NFAT Signaling Blocks Oncogenic H-Ras Induced Autophagy in Primary Human Keratinocytes. Front Cell Dev Biol. 2021;9:720111 pubmed publisher
Dubytska L, Thune R. Early Intracellular Trafficking and Subsequent Activity of Programmed Cell Death in Channel Catfish Macrophages Infected with Edwardsiella ictaluri. Microorganisms. 2020;8: pubmed publisher
An Y, Liu H, Zhou Z, Wang J, Jiang G, Li Z, et al. Sinoporphyrin sodium is a promising sensitizer for photodynamic and sonodynamic therapy in glioma. Oncol Rep. 2020;44:1596-1604 pubmed publisher
Schwarze K, Kribben A, Ritter O, Muller G, Patschan D. Autophagy activation in circulating proangiogenic cells aggravates AKI in type I diabetes mellitus. Am J Physiol Renal Physiol. 2018;315:F1139-F1148 pubmed publisher
Deng X, Meng X, Venardos N, Song R, Yamanaka K, Fullerton D, et al. Autophagy negatively regulates pro-osteogenic activity in human aortic valve interstitial cells. J Surg Res. 2017;218:285-291 pubmed publisher
Javidnia M, Hebron M, Xin Y, Kinney N, Moussa C. Pazopanib Reduces Phosphorylated Tau Levels and Alters Astrocytes in a Mouse Model of Tauopathy. J Alzheimers Dis. 2017;60:461-481 pubmed publisher
Ferreira J, Soares A, Ramalho J, Pereira P, Girao H. K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy. Sci Rep. 2015;5:10210 pubmed publisher
Morgado A, Xavier J, Dionísio P, Ribeiro M, Dias R, Sebastião A, et al. MicroRNA-34a Modulates Neural Stem Cell Differentiation by Regulating Expression of Synaptic and Autophagic Proteins. Mol Neurobiol. 2015;51:1168-83 pubmed publisher
product information
Product Type :
Antibody
Product Name :
LC3A/LC3B Polyclonal Antibody
Catalog # :
PA1-16931
Quantity :
100 uL
Price :
US 496.00
Clonality :
Polyclonal
Purity :
Antigen affinity chromatography
Host :
Rabbit
Reactivity :
Canine, Fish, Human, Mouse, Plant, Rat, Zebrafish
Applications :
ChIP Assay: Assay-Dependent, ELISA: Assay-Dependent, Flow Cytometry: Assay-Dependent, Immunocytochemistry: 1:100-1:300, Immunohistochemistry (Frozen): Assay-Dependent, Immunohistochemistry (Paraffin): 1:200-1:400, Immunoprecipitation: 20 ug/500 ug of lysate, Western Blot: 2 ug/mL
Species :
Canine, Fish, Human, Mouse, Plant, Rat, Zebrafish
Isotype :
IgG
Storage :
-20 C or -80 C if preferred
Description :
Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.
Immunogen :
Synthetic peptide corresponding to a region between residues 25-121 of human LC3 protein.
Format :
Liquid
Applications w/Dilutions :
ChIP Assay: Assay-Dependent, ELISA: Assay-Dependent, Flow Cytometry: Assay-Dependent, Immunocytochemistry: 1:100-1:300, Immunohistochemistry (Frozen): Assay-Dependent, Immunohistochemistry (Paraffin): 1:200-1:400, Immunoprecipitation: 20 ug/500 ug of lysate, Western Blot: 2 ug/mL
Aliases :
1010001C15Rik; 1010001H21Rik; 4922501H04Rik; Atg8; ATG8E; ATG8F; Autophagy-related protein LC3 A; autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 A; autophagy-related ubiquitin-like modifier LC3 B; LC3; LC3a; LC3b; MAP1 light chain 3-like protein 1; MAP1 light chain 3-like protein 2; MAP1A/1B light chain 3 A; MAP1A/1B light chain 3 B; MAP1A/1BLC3; MAP1A/MAP1B LC3 A; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B light chain 3 B; Map1alc3; MAP1BLC3; Map1lc3; Map1lc3a; MAP1LC3B; MAP1LC3B-a; microtubule associated protein 1 light chain 3 alpha; microtubule associated protein 1 light chain 3 beta; microtubule-associated protein 1 light chain 3; microtubule-associated protein 1 light chain 3 alpha; Microtubule-associated protein 1 light chain 3 beta; microtubule-associated protein 1-light chain 3A; microtubule-associated proteins 1A/1B light chain 3; microtubule-associated proteins 1A/1B light chain 3A; Microtubule-associated proteins 1A/1B light chain 3B; Mpl3; wu:fb60g11; zbs559; zgc:56434; zgc:77094
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA