HMGB1 Polyclonal Antibody | Invitrogen PA1-16926 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

HMGB1 Polyclonal Antibody

catalog :

PA1-16926

quantity :

100 µL

price :

US 440.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dogs, bovine, domestic sheep, domestic rabbit

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 5c	Wang G, Xu J, Zhao J, Yin W, Liu D, Chen W, et al. Arf1-mediated lipid metabolism sustains cancer cells and its ablation induces anti-tumor immune responses in mice. Nat Commun. 2020;11:220 pubmed publisher
immunohistochemistry - paraffin section; human; 4 ug/ml; fig 2a	Ladoire S, Senovilla L, Enot D, Ghiringhelli F, Poirier Colame V, Chaba K, et al. Biomarkers of immunogenic stress in metastases from melanoma patients: Correlations with the immune infiltrate. Oncoimmunology. 2016;5:e1160193 pubmed publisher
immunohistochemistry - paraffin section; human; fig 1	Ladoire S, Enot D, Senovilla L, Ghiringhelli F, Poirier Colame V, Chaba K, et al. The presence of LC3B puncta and HMGB1 expression in malignant cells correlate with the immune infiltrate in breast cancer. Autophagy. 2016;12:864-75 pubmed publisher
	Badawi G, Shokr M, Zaki H, Mohamed A. Pentoxifylline prevents epileptic seizure via modulating HMGB1/RAGE/TLR4 signalling pathway and improves memory in pentylenetetrazol kindling rats. Clin Exp Pharmacol Physiol. 2021;48:1111-1124 pubmed publisher
	Beltrán Hernández I, Angelier M, Del Buono D Ondes T, Di Maggio A, Yu Y, Oliveira S. The Potential of Nanobody-Targeted Photodynamic Therapy to Trigger Immune Responses. Cancers (Basel). 2020;12: pubmed publisher
	Shivappagowdar A, Pati S, Narayana C, Ayana R, Kaushik H, Sah R, et al. A small bioactive glycoside inhibits epsilon toxin and prevents cell death. Dis Model Mech. 2019;12: pubmed publisher
	Langer R, Neppl C, Keller M, Schmid R, Tschan M, Berezowska S. Expression Analysis of Autophagy Related Markers LC3B, p62 and HMGB1 Indicate an Autophagy-Independent Negative Prognostic Impact of High p62 Expression in Pulmonary Squamous Cell Carcinomas. Cancers (Basel). 2018;10: pubmed publisher
	Ladoire S, Penault Llorca F, Senovilla L, Dalban C, Enot D, Locher C, et al. Combined evaluation of LC3B puncta and HMGB1 expression predicts residual risk of relapse after adjuvant chemotherapy in breast cancer. Autophagy. 2015;11:1878-90 pubmed publisher
	Vacchelli E, Semeraro M, Enot D, Chaba K, Poirier Colame V, Dartigues P, et al. Negative prognostic impact of regulatory T cell infiltration in surgically resected esophageal cancer post-radiochemotherapy. Oncotarget. 2015;6:20840-50 pubmed

product information

Product Type :

Antibody

Product Name :

HMGB1 Polyclonal Antibody

Catalog # :

PA1-16926

Quantity :

100 µL

Price :

US 440.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Bovine, Canine, Human, Mouse, Ovine, Rabbit, Rat

Applications :

ChIP Assay: 5 µg/mL, ELISA: Assay-Dependent, Flow Cytometry: 1:100, Immunocytochemistry: 0.05 µg/mL, Immunohistochemistry (Paraffin): 1:100-1:250, Western Blot: 0.5-1 µg/mL

Species :

Bovine, Canine, Human, Mouse, Ovine, Rabbit, Rat

Isotype :

IgG

Storage :

4° C, do not freeze

Description :

HMGB1 (High-mobility group box-1) protein was originally described as a nuclear non-histone DNA binding chromosomal protein. However, recent studies indicate that damaged, necrotic cells liberate HMGB1 into the extracellular milieu where it functions as a proinflammatory cytokine. Mouse HMGB1 is expressed as a 215 amino acid single chain polypeptide containing three domains: two tandem-linked positively charged DNA-binding domains (HMG box A, aa 9-79; and box B, aa 89-162), and a negatively charged 30 aa C-terminal acidic tail region. Residues 28 - 44 and 180 - 185 contain a nuclear localization signal (NLS). The cytokine activity of HMGB1 is contained in the B box, while the A box is associated with the helix-loop-helix domain of transcription factors. HMGB1 acts both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, as well as a mediator of T cell-dendritic cell interaction. HMGB1 is secreted and acts to transduce cellular signals through its high affinity receptor, RAGE and possibly, TLR2 and TLR4. HMGB1 is highly conserved and ubiquitous in the nuclei and cytoplasm of nearly all cell types, is a necessary and sufficient mediator of inflammation during sterile and infection-associated responses. HMGB1 also act as DNA nuclear binding protein that has recently been shown to be an early trigger of sterile inflammation in animal models of trauma-hemorrhage via the activation of the Toll-like receptor 4 (TLR4) and the receptor for the advanced glycation endproducts (RAGE). Moreover, HMGB1 is reported that the level of HMGB1 is elevated during sterile tissue injury, infection, lethal endotoxemia or sepsis, collagen-induced arthritis, and ischemia-reperfusion induced tissue injury.

Immunogen :

Synthetic peptide between to amino acids 100-200 of the human HMGB1 protein.

Format :

Liquid

Applications w/Dilutions :

ChIP Assay: 5 µg/mL, ELISA: Assay-Dependent, Flow Cytometry: 1:100, Immunocytochemistry: 0.05 µg/mL, Immunohistochemistry (Paraffin): 1:100-1:250, Western Blot: 0.5-1 µg/mL

Aliases :

Ac2-008; amphoterin; Amphoterin antibody; DEF; DKFZp686A04236; Heparin-binding protein p30; high mobility group 1; high mobility group 1 protein; high mobility group box 1; high mobility group protein 1; High mobility group protein B1; high mobility group protein HMG1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1; high-mobility-group protein; Hmg1; HMG-1; HMG3; HMG3 antibody; hmgb 1; HMGB1; hmgb-1; HMGB1 protein; hypothetical protein; non-histone protein HMG1; p30; RCJMB04_15a21; RP11-550P23.1; SBP-1; Sulfoglucuronyl carbohydrate binding protein; unnamed protein product

more info or order :

Invitrogen product webpage