product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
beta Actin Polyclonal Antibody
catalog :
PA1-16889
quantity :
100 ug
price :
US 471.00
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, dogs
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section
more info or order :
Invitrogen product webpage
citations: 41
Published Application/Species/Sample/DilutionReference
  • western blot; human; loading ...; fig 1c
Mytych J, Romerowicz Misielak M, Koziorowski M. Long-term culture with lipopolysaccharide induces dose-dependent cytostatic and cytotoxic effects in THP-1 monocytes. Toxicol In Vitro. 2017;42:1-9 pubmed publisher
  • western blot; human; 1:2000; loading ...; fig 4b
Bianchi E, Ruberti S, Rontauroli S, Guglielmelli P, Salati S, Rossi C, et al. Role of miR-34a-5p in Hematopoietic Progenitor Cells Proliferation and Fate Decision: Novel Insights into the Pathogenesis of Primary Myelofibrosis. Int J Mol Sci. 2017;18: pubmed publisher
  • western blot; human; loading ...; fig 3e
Mytych J, Wos I, Solek P, Koziorowski M. Protective role of klotho protein on epithelial cells upon co-culture with activated or senescent monocytes. Exp Cell Res. 2017;350:358-367 pubmed publisher
  • western blot; human; 1:1000; loading ...; fig 1c
Collinson Pautz M, Slawin K, Levitt J, Spencer D. MyD88/CD40 Genetic Adjuvant Function in Cutaneous Atypical Antigen-Presenting Cells Contributes to DNA Vaccine Immunogenicity. PLoS ONE. 2016;11:e0164547 pubmed publisher
  • western blot; human; fig 4c
Zini R, Rossi C, Norfo R, Pennucci V, Barbieri G, Ruberti S, et al. miR-382-5p Controls Hematopoietic Stem Cell Differentiation Through the Downregulation of MXD1. Stem Cells Dev. 2016;25:1433-43 pubmed publisher
  • western blot; human; fig 6
Lopes V, Loitto V, Audinot J, Bayat N, Gutleb A, Cristobal S. Dose-dependent autophagic effect of titanium dioxide nanoparticles in human HaCaT cells at non-cytotoxic levels. J Nanobiotechnology. 2016;14:22 pubmed publisher
  • western blot; human; 1:4000; fig 3
Clermont P, Crea F, Chiang Y, Lin D, Zhang A, Wang J, et al. Identification of the epigenetic reader CBX2 as a potential drug target in advanced prostate cancer. Clin Epigenetics. 2016;8:16 pubmed publisher
Martinez G, Cappetta D, Telesca M, Urbanek K, Castaldo G, Dhellemmes M, et al. Cytochalasin D restores nuclear size acting on F-actin and IZUMO1 localization in low-quality spermatozoa. Int J Biol Sci. 2023;19:2234-2255 pubmed publisher
Bi M, Kang W, Sun Y. Expression of HSPA14 in patients with acute HIV-1 infection and its effect on HIV-1 replication. Front Immunol. 2023;14:1123600 pubmed publisher
Sarkisova K, Fedosova E, Shatskova A, Rudenok M, Stanishevskaya V, Slominsky P. Maternal Methyl-Enriched Diet Increases DNMT1, HCN1, and TH Gene Expression and Suppresses Absence Seizures and Comorbid Depression in Offspring of WAG/Rij Rats. Diagnostics (Basel). 2023;13: pubmed publisher
Firatli Y, Firatli E, Loimaranta V, Elmanfi S, G xfc rsoy U. Regulation of gingival keratinocyte monocyte chemoattractant protein-1-induced protein (MCPIP)-1 and mucosa-associated lymphoid tissue lymphoma translocation protein (MALT)-1 expressions by periodontal bacteria, lipopolysaccharide, and interleukin-1&. J Periodontol. 2023;94:130-140 pubmed publisher
Tvrd xe1 E, Kov xe1 x10d J, Ferenczyov xe1 K, Kalo x10d ayov xe1 B, x10e ura x10d ka M, Benko F, et al. Quercetin Ameliorates Testicular Damage in Zucker Diabetic Fatty Rats through Its Antioxidant, Anti-Inflammatory and Anti-Apoptotic Properties. Int J Mol Sci. 2022;23: pubmed publisher
Wang T, Zhou Z, Wang C, Qin Y, Wu L, Hu B, et al. LTBP2 Knockdown Promotes Ferroptosis in Gastric Cancer Cells through p62-Keap1-Nrf2 Pathway. Biomed Res Int. 2022;2022:6532253 pubmed publisher
Kolesnikov A, Lobysheva E, Gnana Prakasam J, Kefalov V, Kisselev O. Regulation of rod photoreceptor function by farnesylated G-protein γ-subunits. PLoS ONE. 2022;17:e0272506 pubmed publisher
Stanaszek L, Rogujski P, Drela K, Fiedorowicz M, Walczak P, Lukomska B, et al. Transplantation of Human Glial Progenitors to Immunodeficient Neonatal Mice with Amyotrophic Lateral Sclerosis (SOD1/rag2). Antioxidants (Basel). 2022;11: pubmed publisher
Solek P, Mytych J, Sujkowska E, Grzegorczyk M, Jasiewicz P, Sowa Kucma M, et al. Trade-offs between male fertility reduction and selected growth factors or the klotho response in a lipopolysaccharide-dependent mouse model. Toxicol Res. 2022;38:175-186 pubmed publisher
Tallino S, Winslow W, Bartholomew S, Velázquez R. Temporal and brain region-specific elevations of soluble Amyloid-β40-42 in the Ts65Dn mouse model of Down syndrome and Alzheimer's disease. Aging Cell. 2022;21:e13590 pubmed publisher
Poria D, Sun C, Santeford A, Kielar M, Apte R, Kisselev O, et al. EML1 is essential for retinal photoreceptor migration and survival. Sci Rep. 2022;12:2897 pubmed publisher
Correll V, Otto J, Risi C, Main B, Boutros P, Kislinger T, et al. Optimization of small extracellular vesicle isolation from expressed prostatic secretions in urine for in-depth proteomic analysis. J Extracell Vesicles. 2022;11:e12184 pubmed publisher
Winslow W, McDonough I, Tallino S, Decker A, Vural A, Velázquez R. IntelliCage Automated Behavioral Phenotyping Reveals Behavior Deficits in the 3xTg-AD Mouse Model of Alzheimer's Disease Associated With Brain Weight. Front Aging Neurosci. 2021;13:720214 pubmed publisher
Liu Q, Chen F, Yang T, Su J, Song S, Fu Z, et al. Aged-related Function Disorder of Liver is Reversed after Exposing to Young Milieu via Conversion of Hepatocyte Ploidy. Aging Dis. 2021;12:1238-1251 pubmed publisher
Dash R, Wen J, Zaino A, Morel S, Chau L, Wechsler Reya R, et al. Structure-based virtual screening identifies an 8-hydroxyquinoline as a small molecule GLI1 inhibitor. Mol Ther Oncolytics. 2021;20:265-276 pubmed publisher
Kumar M, Shah D, Giridharan M, Yadav N, Manjithaya R, Clement J. Spatiotemporal analysis of soluble aggregates and autophagy markers in the R6/2 mouse model. Sci Rep. 2021;11:96 pubmed publisher
Sanders J, Freeman T, Xu Y, Golloshi R, Stallard M, Hill A, et al. Radiation-induced DNA damage and repair effects on 3D genome organization. Nat Commun. 2020;11:6178 pubmed publisher
Liang X, Gao J, Wang Q, Hou S, Wu C. ECRG4 Represses Cell Proliferation and Invasiveness via NFIC/OGN/NF-κB Signaling Pathway in Bladder Cancer. Front Genet. 2020;11:846 pubmed publisher
Ghosh R, Bryant D, Arivett B, Smith S, Altman E, Kline P, et al. An acidic polysaccharide (AGC3) isolated from North American ginseng (Panax quinquefolius) suspension culture as a potential immunomodulatory nutraceutical. Curr Res Food Sci. 2020;3:207-216 pubmed publisher
Junkunlo K, S xf6 derh xe4 ll K, S xf6 derh xe4 ll I. Transglutaminase 1 and 2 are localized in different blood cells in the freshwater crayfish Pacifastacus leniusculus. Fish Shellfish Immunol. 2020;104:83-91 pubmed publisher
Tu E, Chen Q, Tan L, Wang Y. Dl-3-n-Butylphthalide promotes neovascularization and neurological recovery in a rat model of intracerebral hemorrhage. BMC Neurosci. 2020;21:24 pubmed publisher
Yang Y, Luo M, Zhang K, Zhang J, Gao T, Connell D, et al. Nedd4 ubiquitylates VDAC2/3 to suppress erastin-induced ferroptosis in melanoma. Nat Commun. 2020;11:433 pubmed publisher
Mytych J, Sołek P, Będzińska A, Rusinek K, Warzybok A, Tabęcka Łonczyńska A, et al. Towards Age-Related Anti-Inflammatory Therapy: Klotho Suppresses Activation of ER and Golgi Stress Response in Senescent Monocytes. Cells. 2020;9: pubmed publisher
Du J, Ren W, Yao F, Wang H, Zhang K, Luo M, et al. YY1 cooperates with TFEB to regulate autophagy and lysosomal biogenesis in melanoma. Mol Carcinog. 2019;58:2149-2160 pubmed publisher
Solek P, Koszla O, Mytych J, Badura J, Chelminiak Z, Cuprys M, et al. Neuronal life or death linked to depression treatment: the interplay between drugs and their stress-related outcomes relate to single or combined drug therapies. Apoptosis. 2019;24:773-784 pubmed publisher
Hijmans J, Stockelman K, Garcia V, Levy M, Brewster L, Bammert T, et al. Circulating Microparticles Are Elevated in Treated HIV -1 Infection and Are Deleterious to Endothelial Cell Function. J Am Heart Assoc. 2019;8:e011134 pubmed publisher
Rossi C, Zini R, Rontauroli S, Ruberti S, Prudente Z, Barbieri G, et al. Role of TGF-β1/miR-382-5p/SOD2 axis in the induction of oxidative stress in CD34+ cells from primary myelofibrosis. Mol Oncol. 2018;12:2102-2123 pubmed publisher
de Dios O, Gavela Pérez T, Aguado Roncero P, Pérez Tejerizo G, Ricote M, Gonzalez N, et al. C-reactive protein expression in adipose tissue of children with acute appendicitis. Pediatr Res. 2018;84:564-567 pubmed publisher
Driedonks T, Van der Grein S, Ariyurek Y, Buermans H, Jekel H, Chow F, et al. Immune stimuli shape the small non-coding transcriptome of extracellular vesicles released by dendritic cells. Cell Mol Life Sci. 2018;75:3857-3875 pubmed publisher
Tabecka Lonczynska A, Mytych J, Solek P, Kulpa Greszta M, Koziorowski M. Melatonin receptors subtypes (MT1 and MT2) in the uterus masculinus of mature male european bison. Biological and seasonal reproductive role. J Physiol Pharmacol. 2018;69:67-73 pubmed publisher
Rutkowska A, Sailer A, Dev K. EBI2 receptor regulates myelin development and inhibits LPC-induced demyelination. J Neuroinflammation. 2017;14:250 pubmed publisher
Salati S, Salvestrini V, Carretta C, Genovese E, Rontauroli S, Zini R, et al. Deregulated expression of miR-29a-3p, miR-494-3p and miR-660-5p affects sensitivity to tyrosine kinase inhibitors in CML leukemic stem cells. Oncotarget. 2017;8:49451-49469 pubmed publisher
Rontauroli S, Norfo R, Pennucci V, Zini R, Ruberti S, Bianchi E, et al. miR-494-3p overexpression promotes megakaryocytopoiesis in primary myelofibrosis hematopoietic stem/progenitor cells by targeting SOCS6. Oncotarget. 2017;8:21380-21397 pubmed publisher
Bianchi E, Bulgarelli J, Ruberti S, Rontauroli S, Sacchi G, Norfo R, et al. MYB controls erythroid versus megakaryocyte lineage fate decision through the miR-486-3p-mediated downregulation of MAF. Cell Death Differ. 2015;22:1906-21 pubmed publisher
product information
Product Type :
Antibody
Product Name :
beta Actin Polyclonal Antibody
Catalog # :
PA1-16889
Quantity :
100 ug
Price :
US 471.00
Clonality :
Polyclonal
Purity :
Antigen affinity chromatography
Host :
Rabbit
Reactivity :
Canine, Human, Mouse, Non-human primate
Applications :
Immunocytochemistry: 5 ug/mL, Immunohistochemistry (Paraffin): 1:2000-1:10000, Immunoprecipitation: Assay-Dependent, Western Blot: 1:2,000-1:10,000
Species :
Canine, Human, Mouse, Non-human primate
Isotype :
IgG
Storage :
Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.
Description :
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
Immunogen :
N-terminus of human beta actin using the numbering given in Swiss-prot entry P60709 and is highly conserved with gamma actin
Format :
Liquid
Applications w/Dilutions :
Immunocytochemistry: 5 ug/mL, Immunohistochemistry (Paraffin): 1:2000-1:10000, Immunoprecipitation: Assay-Dependent, Western Blot: 1:2,000-1:10,000
Aliases :
0610041G09Rik; AA959943; AAT6; ACT; Act4; Act-4; ACT-5; ACTA; acta1; acta1b; ACTA2; Acta-2; ACTA3; actb; actb.L; actb1; actba; ACTC; actc1; Actc-1; actc1b; ACTE; Actg; ACTG1; Actg2; ACTGE; actin; actin alpha 1; actin alpha 1, skeletal muscle; actin alpha 1, skeletal muscle b; actin alpha 2, smooth muscle; actin alpha cardiac; actin alpha cardiac 1; actin alpha cardiac muscle 1; actin alpha cardiac muscle 1b; actin beta; actin gamma 1; actin gamma 2, smooth muscle; actin, alpha 1, skeletal muscle; actin, alpha 1b, skeletal muscle; actin, alpha 2, smooth muscle, aorta; Actin, alpha cardiac muscle 1; Actin, alpha cardiac muscle 1, intermediate form; actin, alpha cardiac muscle 1b; actin, alpha skeletal muscle; Actin, alpha skeletal muscle, intermediate form; actin, alpha, cardiac 1; actin, alpha, cardiac muscle; actin, alpha, cardiac muscle 1; actin, alpha, vascular smooth muscle; actin, aortic smooth muscle; Actin, aortic smooth muscle, intermediate form; actin, beta; actin, beta 1; actin, beta L homeolog; actin, beta, cytoplasmic; actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; Actin, cytoplasmic 2; Actin, cytoplasmic 2, N-terminally processed; actin, gamma 1; actin, gamma 2, smooth muscle, enteric; actin, gamma, cytoplasmic 1; actin, gamma-enteric smooth muscle; Actin, gamma-enteric smooth muscle, intermediate form; actin-like protein; Actl; ACTL3; Acts; ACTSA; ACTSG; Actsk-1; Actvs; Actx; AL023024; alpha actin 1; alpha-actin cardiac; alpha-actin-1; Alpha-actin-2; alpha-actin-3; alphac-actin; Alpha-cardiac actin; alphaSMA; alpha-smooth muscle actin; ASD5; ASMA; a-SMA; Bact; Bact; actin; B-actin; bactin1; bactin1 protein; bactzf; B-ACTZF; beta actin; beta cytoskeletal actin; beta-actin; beta-actin FE-3; beta-actin-1; BRWS1; BRWS2; cardiac muscle alpha actin 1; cardiofunk; cell growth-inhibiting gene 46 protein; Cfk; CFTD; CFTD1; CFTDM; CMD1R; CMH11; cytoplasmic 1; cytoplasmic beta-actin; cytoskeletal beta actin; cytoskeletal gamma-actin; cytoskeletal protein; deafness, autosomal dominant 20; deafness, autosomal dominant 26; DFNA20; DFNA26; E430023M04Rik; E51; epididymis luminal protein 176; fa27h01; fb83f06; gamma non-muscle actin; gamma-2-actin; Gamma-actin; gamma-enteric smooth muscle actin; GIG46; HEL-176; hm:zeh0631; I79_002310; I79_013242; I79_019066; LVNC4; MPFD; MYMY5; NEM1; NEM2; NEM3; nemaline myopathy type 3; PS1TP5-binding protein 1; PS1TP5BP1; SHPM; similar to beta actin; skeletal alpha actin; skeletal alpha1 actin; sma; SMalphaA; SMGA; smooth muscle alpha-actin; smooth muscle gamma-actin; vascular smooth muscle alpha-actin; VSCM; wu:fa27h01; wu:fb63d03; wu:fb83f06; wu:fd18f05; XELAEV_18045052mg; zeh0631
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA