NEFM Polyclonal Antibody | Invitrogen PA1-16758 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

NEFM Polyclonal Antibody

catalog :

PA1-16758

quantity :

250 uL

price :

US 514.00

clonality :

polyclonal

host :

chicken

conjugate :

nonconjugated

reactivity :

human, mouse, rat, chicken, bovine, pigs , domestic horse

application :

western blot, immunocytochemistry

citations: 2

Reference
Junghans M, John F, Cihankaya H, Schliebs D, Winklhofer K, Bader V, et al. ROS scavengers decrease γH2ax spots in motor neuronal nuclei of ALS model mice in vitro. Front Cell Neurosci. 2022;16:963169 pubmed publisher
Potratz M, Zaeck L, Weigel C, Klein A, Freuling C, Muller T, et al. Neuroglia infection by rabies virus after anterograde virus spread in peripheral neurons. Acta Neuropathol Commun. 2020;8:199 pubmed publisher

product information

Product Type :

Antibody

Product Name :

NEFM Polyclonal Antibody

Catalog # :

PA1-16758

Quantity :

250 uL

Price :

US 514.00

Clonality :

Polyclonal

Purity :

IgY fraction

Host :

Chicken

Reactivity :

Bovine, Chicken, Drosophila, Equine, Human, Mouse, Porcine, Rat

Applications :

Immunocytochemistry: 1:1,000, Immunohistochemistry - Free Floating: 1:1,000, Western Blot: 1:5,000-1:10,000

Species :

Bovine, Chicken, Drosophila, Equine, Human, Mouse, Porcine, Rat

Isotype :

IgY

Storage :

Store at 4 C short term. For long term storage, store at -20 C, avoiding freeze/thaw cycles.

Description :

Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.

Immunogen :

Recombinant construct containing the C-terminus of the human sequence (amino acids 708-877) expressed in and purified from E. coli.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:1,000, Immunohistochemistry - Free Floating: 1:1,000, Western Blot: 1:5,000-1:10,000

Aliases :

160 kDa neurofilament protein; major neuronal intermediate filament subunit; medium neurofilament subunit; middle molecular weight neurofilament protein NF-M(2); Nef3; Nefm; nef-m; nefm.S; nefm-a; nefm-b; Neurofilament 3; neurofilament 3 (150kDa medium); neurofilament 3, medium; neurofilament M subunit; neurofilament medium; neurofilament medium polypeptide; neurofilament medium S homeolog; neurofilament medium tail domain; neurofilament protein M; Neurofilament protein, middle polypeptide; neurofilament triplet M protein; neurofilament, medium polypeptide; neurofilament, medium polypeptide 150kDa; neurofilament, medium polypeptide S homeolog; neurofilament-3 (150 kD medium); neurofilament-M; neurofilament-M subunit; neuronal intermediate filament; NF160; NF165; NFM; NF-M; NF-M c-terminus; NF-M protein; NMC; XELAEV_18020229mg