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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
HIF1A Polyclonal Antibody
catalog :
PA1-16601
quantity :
100 uL
price :
US 494.00
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
domestic goat, Chinese hamsters, human, mouse, rat, dogs, pigs , domestic rabbit
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section
more info or order :
Invitrogen product webpage
citations: 32
Published Application/Species/Sample/DilutionReference
  • western blot; mouse; 1:1000; loading ...; fig 1b
Xiao N, Yang L, Yang Y, Liu L, Li J, Liu B, et al. Ginsenoside Rg5 Inhibits Succinate-Associated Lipolysis in Adipose Tissue and Prevents Muscle Insulin Resistance. Front Pharmacol. 2017;8:43 pubmed publisher
  • western blot; mouse; fig 3
Zhao W, Li A, Feng X, Hou T, Liu K, Liu B, et al. Metformin and resveratrol ameliorate muscle insulin resistance through preventing lipolysis and inflammation in hypoxic adipose tissue. Cell Signal. 2016;28:1401-11 pubmed publisher
Ruan Z, Yin H, Wan T, Lin Z, Zhao S, Long H, et al. Metformin accelerates bone fracture healing by promoting type H vessel formation through inhibition of YAP1/TAZ expression. Bone Res. 2023;11:45 pubmed publisher
Zeng J, Wang Y, Zhu M, Wu M, Zhou Y, Wang Q, et al. Neutrophil extracellular traps boost laser-induced mouse choroidal neovascularization through the activation of the choroidal endothelial cell TLR4/HIF-1α pathway. FEBS J. 2023;290:5395-5410 pubmed publisher
Wu J, Wang X, Chen L, Wang J, Zhang J, Tang J, et al. Oxygen microcapsules improve immune checkpoint blockade by ameliorating hypoxia condition in pancreatic ductal adenocarcinoma. Bioact Mater. 2023;20:259-270 pubmed publisher
Do T, Lau J, Sun C, Liu S, Kha K, Lim S, et al. Hypoxia deactivates epigenetic feedbacks via enzyme-derived clicking proteolysis-targeting chimeras. Sci Adv. 2022;8:eabq2216 pubmed publisher
Yong J, Gr xf6 ger S, von Bremen J, Meyle J, Ruf S. Immunorthodontics: PD-L1, a Novel Immunomodulator in Cementoblasts, Is Regulated by HIF-1α under Hypoxia. Cells. 2022;11: pubmed publisher
Salaroglio I, Belisario D, Akman M, La Vecchia S, Godel M, Anobile D, et al. Mitochondrial ROS drive resistance to chemotherapy and immune-killing in hypoxic non-small cell lung cancer. J Exp Clin Cancer Res. 2022;41:243 pubmed publisher
Górowska Wójtowicz E, Duliban M, Kotula Balak M, Bilinska B. Modulatory Effects of Estradiol and Its Mixtures with Ligands of GPER and PPAR on MAPK and PI3K/Akt Signaling Pathways and Tumorigenic Factors in Mouse Testis Explants and Mouse Tumor Leydig Cells. Biomedicines. 2022;10: pubmed publisher
McKenna M, Filteau J, Butler B, Sluis K, Chungyoun M, Schimek N, et al. Organotypic whole hemisphere brain slice models to study the effects of donor age and oxygen-glucose-deprivation on the extracellular properties of cortical and striatal tissue. J Biol Eng. 2022;16:14 pubmed publisher
Huy T, Nguyen T, Reyes A, Kim H, Min W, Lee H, et al. Cobalt (II) Chloride Regulates the Invasion and Survival of Brucella abortus 544 in RAW 264.7 Cells and B6 Mice. Pathogens. 2022;11: pubmed publisher
Park M, Kim H, Shin H, Lee S, Hwang D, Lew H. Human Pluripotent Stem Cell-Derived Neural Progenitor Cells Promote Retinal Ganglion Cell Survival and Axon Recovery in an Optic Nerve Compression Animal Model. Int J Mol Sci. 2021;22: pubmed publisher
Kim H, Park M, Byun H, Kim M, Kim B, Yu L, et al. LW1497, an Inhibitor of Malate Dehydrogenase, Suppresses TGF-β1-Induced Epithelial-Mesenchymal Transition in Lung Cancer Cells by Downregulating Slug. Antioxidants (Basel). 2021;10: pubmed publisher
Zhao L, Law N, Gomez N, Son J, Gao Y, Liu X, et al. Obesity Impairs Embryonic Myogenesis by Enhancing BMP Signaling within the Dermomyotome. Adv Sci (Weinh). 2021;8:e2102157 pubmed publisher
Potor L, Hendrik Z, Patsalos A, Katona x, M xe9 hes G, P xf3 liska S, et al. Oxidation of Hemoglobin Drives a Proatherogenic Polarization of Macrophages in Human Atherosclerosis. Antioxid Redox Signal. 2021;35:917-950 pubmed publisher
do Monte F, Ahuja N, Awad K, Pan Z, Young S, Kim H, et al. Silicon Oxynitrophosphide Nanoscale Coating Enhances Antioxidant Marker-Induced Angiogenesis During in vivo Cranial Bone-Defect Healing. JBMR Plus. 2021;5:e10425 pubmed publisher
Madsen A, Krause J, H xf6 ppner G, Hirt M, Tan W, Lim I, et al. Hypertrophic signaling compensates for contractile and metabolic consequences of DNA methyltransferase 3A loss in human cardiomyocytes. J Mol Cell Cardiol. 2021;154:115-123 pubmed publisher
Madsen A, Höppner G, Krause J, Hirt M, Laufer S, Schweizer M, et al. An Important Role for DNMT3A-Mediated DNA Methylation in Cardiomyocyte Metabolism and Contractility. Circulation. 2020;142:1562-1578 pubmed publisher
Sayama S, Song A, Brown B, Couturier J, Cai X, Xu P, et al. Maternal erythrocyte ENT1-mediated AMPK activation counteracts placental hypoxia and supports fetal growth. JCI Insight. 2020;5: pubmed publisher
Kullmann J, Trivedi N, Howell D, Laumonnerie C, Nguyen V, Banerjee S, et al. Oxygen Tension and the VHL-Hif1α Pathway Determine Onset of Neuronal Polarization and Cerebellar Germinal Zone Exit. Neuron. 2020;106:607-623.e5 pubmed publisher
Sivaraj K, Dharmalingam B, Mohanakrishnan V, Jeong H, Kato K, Schröder S, et al. YAP1 and TAZ negatively control bone angiogenesis by limiting hypoxia-inducible factor signaling in endothelial cells. elife. 2020;9: pubmed publisher
Hackler L, Gyuris M, Huzian O, Alföldi R, Szebeni G, Madácsi R, et al. Enantioselective Synthesis of 8-Hydroxyquinoline Derivative, Q134 as a Hypoxic Adaptation Inducing Agent. Molecules. 2019;24: pubmed publisher
Potharaju M, Mathavan A, Mangaleswaran B, Patil S, John R, Ghosh S, et al. Clinicopathological Analysis of HIF-1alpha and TERT on Survival Outcome in Glioblastoma Patients: A Prospective, Single Institution Study. J Cancer. 2019;10:2397-2406 pubmed publisher
Hsu H, Liao P, Cheng Y, Huang S, Wu C, Li C, et al. Chloramphenicol Induces Autophagy and Inhibits the Hypoxia Inducible Factor-1 Alpha Pathway in Non-Small Cell Lung Cancer Cells. Int J Mol Sci. 2019;20: pubmed publisher
Evans M, Huang P, Iwamoto Y, Ibsen K, Chan E, Hitomi Y, et al. Macrophage-mediated delivery of light activated nitric oxide prodrugs with spatial, temporal and concentration control. Chem Sci. 2018;9:3729-3741 pubmed publisher
Mira E, Carmona Rodríguez L, Pérez Villamil B, Casas J, Fernández Aceñero M, Martínez Rey D, et al. SOD3 improves the tumor response to chemotherapy by stabilizing endothelial HIF-2α. Nat Commun. 2018;9:575 pubmed publisher
Meng Q, Qin Y, Deshpande M, Kashiwabuchi F, Rodrigues M, Lu Q, et al. Hypoxia-Inducible Factor-Dependent Expression of Angiopoietin-Like 4 by Conjunctival Epithelial Cells Promotes the Angiogenic Phenotype of Pterygia. Invest Ophthalmol Vis Sci. 2017;58:4514-4523 pubmed publisher
Amofa D, Hulin A, Nakada Y, Sadek H, Yutzey K. Hypoxia promotes primitive glycosaminoglycan-rich extracellular matrix composition in developing heart valves. Am J Physiol Heart Circ Physiol. 2017;313:H1143-H1154 pubmed publisher
Iguchi N, Malykhina A, Wilcox D. Inhibition of HIF Reduces Bladder Hypertrophy and Improves Bladder Function in Murine Model of Partial Bladder Outlet Obstruction. J Urol. 2016;195:1250-6 pubmed publisher
Huang J, Liu L, Feng M, An S, Zhou M, Li Z, et al. Effect of CoClâ‚‚ on fracture repair in a rat model of bone fracture. Mol Med Rep. 2015;12:5951-6 pubmed publisher
Xu X, Tan X, Tampe B, Sánchez E, Zeisberg M, Zeisberg E. Snail Is a Direct Target of Hypoxia-inducible Factor 1α (HIF1α) in Hypoxia-induced Endothelial to Mesenchymal Transition of Human Coronary Endothelial Cells. J Biol Chem. 2015;290:16653-64 pubmed publisher
Guven M, Sehitoglu M, Yüksel Y, Tokmak M, Aras A, Akman T, et al. The Neuroprotective Effect of Coumaric Acid on Spinal Cord Ischemia/Reperfusion Injury in Rats. Inflammation. 2015;38:1986-95 pubmed publisher
product information
Product Type :
Antibody
Product Name :
HIF1A Polyclonal Antibody
Catalog # :
PA1-16601
Quantity :
100 uL
Price :
US 494.00
Clonality :
Polyclonal
Purity :
Antigen affinity chromatography
Host :
Rabbit
Reactivity :
Canine, Fish, Goat, Hamster, Human, Mouse, Non-human primate, Porcine, Rabbit, Rat
Applications :
ChIP Assay: 1:10-1:500, Flow Cytometry: 1:10-1:1,000, Immunocytochemistry: 1:100, Immunohistochemistry (Frozen): 5-10 ug/mL, Immunohistochemistry (Paraffin): 5-10 ug/mL, Immunoprecipitation: 1:10-1:500, Western Blot: 1-2 ug/mL
Species :
Canine, Fish, Goat, Hamster, Human, Mouse, Non-human primate, Porcine, Rabbit, Rat
Isotype :
IgG
Storage :
-20 C
Description :
HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.
Immunogen :
A fusion protein including residues 530-825 of the mouse HIF-1 alpha protein.
Format :
Liquid
Applications w/Dilutions :
ChIP Assay: 1:10-1:500, Flow Cytometry: 1:10-1:1,000, Immunocytochemistry: 1:100, Immunohistochemistry (Frozen): 5-10 ug/mL, Immunohistochemistry (Paraffin): 5-10 ug/mL, Immunoprecipitation: 1:10-1:500, Western Blot: 1-2 ug/mL
Aliases :
AA959795; ARNT interacting protein; ARNT2; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; bHLHe78; Class E basic helix-loop-helix protein 78; HIF; hif 1; hif 1a; HIF1; HIF1 alpha; HIF-1 alpha; HIF1a; hif-1a; hif1a.L; HIF1alpha; HIF-1alpha; HIF-1-alpha; HIF1-alpha; HIF1-alpha protein; hypoxia inducible factor 1 alpha; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1 alpha subunit L homeolog; hypoxia inducible factor 1 subunit alpha; hypoxia inducible factor 1 subunit alpha L homeolog; hypoxia inducible factor 1, alpha subunit; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia inducible factor-1 alpha; hypoxia-inducible factor 1 alpha; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1 alpha subunit; hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) L homeolog; hypoxia-inducible factor 1-alpha; hypoxia-inducible factor-1 alpha; hypoxia-inducible factor1alpha; member of PAS protein 1; member of PAS superfamily 1; MOP1; PAS domain-containing protein 8; PASD8; XELAEV_18039792mg; XELAEV_18041137mg; xhif1a
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA