EGFR Polyclonal Antibody | Invitrogen PA1-1110 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

EGFR Polyclonal Antibody

catalog :

PA1-1110

quantity :

100 µg

price :

US 480.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

Invitrogen product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; 1:1000; fig 6c	Coudert L, Osseni A, Gangloff Y, Schaeffer L, Leblanc P. The ESCRT-0 subcomplex component Hrs/Hgs is a master regulator of myogenesis via modulation of signaling and degradation pathways. BMC Biol. 2021;19:153 pubmed publisher
western blot; human; 1 ug/ml; loading ...; fig 1c	Quinney K, Frankel E, Shankar R, Kasberg W, Luong P, Audhya A. Growth factor stimulation promotes multivesicular endosome biogenesis by prolonging recruitment of the late-acting ESCRT machinery. Proc Natl Acad Sci U S A. 2019;116:6858-6867 pubmed publisher
immunocytochemistry; human; loading ...; fig 1c	Zhao B, Hu W, Kumar S, Gonyo P, Rana U, Liu Z, et al. The Nogo-B receptor promotes Ras plasma membrane localization and activation. Oncogene. 2017;36:3406-3416 pubmed publisher
	Wang M, Zhang Z, Liu J, Song M, Zhang T, Chen Y, et al. Gefitinib and fostamatinib target EGFR and SYK to attenuate silicosis: a multi-omics study with drug exploration. Signal Transduct Target Ther. 2022;7:157 pubmed publisher
	Gireud Goss M, Reyes S, Tewari R, Patrizz A, Howe M, Kofler J, et al. The ubiquitin ligase UBE4B regulates amyloid precursor protein ubiquitination, endosomal trafficking, and amyloid β42 generation and secretion. Mol Cell Neurosci. 2020;108:103542 pubmed publisher
	Shi T, Fujita K, Gong J, Nakahara M, Iwama H, Liu S, et al. Aspirin inhibits hepatocellular carcinoma cell proliferation in vitro and in vivo via inducing cell cycle arrest and apoptosis. Oncol Rep. 2020;44:457-468 pubmed publisher
	Hänsch S, Spona D, Murra G, Köhrer K, Subtil A, Furtado A, et al. Chlamydia-induced curvature of the host-cell plasma membrane is required for infection. Proc Natl Acad Sci U S A. 2020;117:2634-2644 pubmed publisher
	Li L, Wang D. MicroRNA‑128‑b regulates epidermal growth factor receptor expression in non‑small cell lung cancer. Mol Med Rep. 2019;20:4803-4810 pubmed publisher
	Carnevale D, Facchinello N, Iodice D, Bizzotto D, Perrotta M, De Stefani D, et al. Loss of EMILIN-1 Enhances Arteriolar Myogenic Tone Through TGF-β (Transforming Growth Factor-β)-Dependent Transactivation of EGFR (Epidermal Growth Factor Receptor) and Is Relevant for Hypertension in Mice and Humans. Arterioscler Thromb Vasc Biol. 2018;38:2484-2497 pubmed publisher
	Nam S, Kim D, Lee D, Lee H, Song D, Jung J, et al. Lysyl-tRNA synthetase-expressing colon spheroids induce M2 macrophage polarization to promote metastasis. J Clin Invest. 2018;128:5034-5055 pubmed publisher
	Gireud Goss M, Reyes S, Wilson M, Farley M, Memarzadeh K, Srinivasan S, et al. Distinct mechanisms enable inward or outward budding from late endosomes/multivesicular bodies. Exp Cell Res. 2018;372:1-15 pubmed publisher
	Vermillion M, Ursin R, Kuok D, Vom Steeg L, Wohlgemuth N, Hall O, et al. Production of amphiregulin and recovery from influenza is greater in males than females. Biol Sex Differ. 2018;9:24 pubmed publisher
	Gradzka S, Thomas O, Kretz O, Haimovici A, Vasilikos L, Wong W, et al. Inhibitor of apoptosis proteins are required for effective fusion of autophagosomes with lysosomes. Cell Death Dis. 2018;9:529 pubmed publisher
	Wierzbicki M, Jaworski S, Kutwin M, Grodzik M, Strojny B, Kurantowicz N, et al. Diamond, graphite, and graphene oxide nanoparticles decrease migration and invasiveness in glioblastoma cell lines by impairing extracellular adhesion. Int J Nanomedicine. 2017;12:7241-7254 pubmed publisher
	Mölleken K, Hegemann J. Acquisition of Rab11 and Rab11-Fip2-A novel strategy for Chlamydia pneumoniae early survival. PLoS Pathog. 2017;13:e1006556 pubmed publisher
	Fausther M, Goree J, Lavoie Ã, Graham A, SÃ©vigny J, Dranoff J. Establishment and characterization of rat portal myofibroblast cell lines. PLoS ONE. 2015;10:e0121161 pubmed publisher
	Li N, Ramil C, Lim R, Lin Q. A genetically encoded alkyne directs palladium-mediated protein labeling on live mammalian cell surface. ACS Chem Biol. 2015;10:379-84 pubmed publisher

image

image 1 :

Immunohistochemistry was performed on cancer biopsies of deparaffinized human Lung cancer tissue. To expose target proteins high pressure heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes. Following antigen retrieval endogenous peroxidase activity was quenched with 3% hydrogen peroxide for 10 minutes at room-temp. Tissues were then washed in PBS and blocked in 10% normal goat serum for 20 minutes at room temperature. Tissues were probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing EGFR (Product # PA1-1110) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBS. Colorimetric detection was performed using metal enhanced DAB. Image is displayed at 40X magnification. Results demonstrate strong membranous localization of EGFR.

image 2 :

Immunofluorescent analysis of EGFR (green) in untreated HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product #31873) for 15 minutes at room temperature. Cells were probed with a rabbit polyclonal antibody recognizing EGFR (Product # PA1-1110), at a dilution of 1:500 for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei were stained with Hoechst 33342 dye (Product# 62249). Images were taken on a Thermo Scientific ArrayScan at 20X magnification.

image 3 :

Western blot detection of EGFR from human HeLa cells using PA1-1110.

product information

Product Type :

Antibody

Product Name :

EGFR Polyclonal Antibody

Catalog # :

PA1-1110

Quantity :

100 µg

Price :

US 480.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse, Non-human primate, Rat

Applications :

Immunocytochemistry: 1 µg/mL, Immunohistochemistry (Paraffin): 1:200, Immunoprecipitation: 2 µg, Western Blot: 1:500

Species :

Human, Mouse, Non-human primate, Rat

Isotype :

IgG

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

Immunogen :

Synthetic peptide corresponding to residues C L(1198) R V A P Q S S E F I G A(1210) of human EGFR.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1 µg/mL, Immunohistochemistry (Paraffin): 1:200, Immunoprecipitation: 2 µg, Western Blot: 1:500

Aliases :

2.7.10.1; 9030024J15Rik; AI552599; avian erythroblastic leukemia viral (v-erbB) oncogene homolog; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; egf receptor; Egfr; EGFR-related peptide; epidermal growth factor receptor; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); ERBB; ERBB1; ErbB-1; erb-b2 receptor tyrosine kinase 1; Errb1; Errp; HER1; kinase EGFR; mENA; NISBD2; Oncogene ERBB; PIG61; Proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; Urogastrone; wa2; wa-2; Wa5; waved 2

more info or order :

Invitrogen product webpage