Phospho-IRS1 (Ser307) Polyclonal Antibody | Invitrogen PA1-1054 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Phospho-IRS1 (Ser307) Polyclonal Antibody

catalog :

PA1-1054

quantity :

100 µg

price :

US 470.00

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

human, mouse

application :

western blot, immunocytochemistry

more info or order :

Invitrogen product webpage

citations: 3

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:300; fig 4h	Mahata T, Sengar A, Basak M, Das K, Pramanick A, Verma S, et al. Hepatic Regulator of G Protein Signaling 6 (RGS6) drives non-alcoholic fatty liver disease by promoting oxidative stress and ATM-dependent cell death. Redox Biol. 2021;46:102105 pubmed publisher
	Xu M, Tan J, Dong W, Zou B, Teng X, Zhu L, et al. The E3 ubiquitin-protein ligase Trim31 alleviates non-alcoholic fatty liver disease by targeting Rhbdf2 in mouse hepatocytes. Nat Commun. 2022;13:1052 pubmed publisher
	Sanap A, Bhonde R, Joshi K. Mesenchymal stem cell conditioned medium ameliorates diabetic serum-induced insulin resistance in 3T3-L1 cells. Chronic Dis Transl Med. 2021;7:47-56 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Phospho-IRS1 (Ser307) Polyclonal Antibody

Catalog # :

PA1-1054

Quantity :

100 µg

Price :

US 470.00

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse

Applications :

Immunocytochemistry: 1-2 µg/mL, Western Blot: 1-3 µg/mL

Species :

Human, Mouse

Isotype :

IgG

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.

Immunogen :

Synthetic phosphopeptide corresponding to residues E(301) S I T A T (pS) P A S M V G G K(315) of human IRS-1.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1-2 µg/mL, Western Blot: 1-3 µg/mL

Aliases :

G972R; HIRS-1; Insulin receptor substrate 1; IRS1; IRS-1; IRS1IRM; pp185

more info or order :

Invitrogen product webpage