EBP50 Polyclonal Antibody | Invitrogen PA1-090 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

EBP50 Polyclonal Antibody

catalog :

PA1-090

quantity :

100 µg

price :

US 480

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry knockout validation

more info or order :

Invitrogen product webpage

citations: 14

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry knockout validation; mouse; 1:3200; fig 2A immunohistochemistry - paraffin section; mouse; 1:3200; fig 2A	Georgescu M, Gagea M, Cote G. NHERF1/EBP50 Suppresses Wnt-?-Catenin Pathway-Driven Intestinal Neoplasia. Neoplasia. 2016;18:512-23 pubmed publisher
immunohistochemistry; mouse; 1:200; loading ...; fig 1a western blot; mouse; 1:2000; loading ...; fig 2b	Shang P, Stepicheva N, Teel K, McCauley A, Fitting C, Hose S, et al. βA3/A1-crystallin regulates apical polarity and EGFR endocytosis in retinal pigmented epithelial cells. Commun Biol. 2021;4:850 pubmed publisher
immunohistochemistry; human; 1:500; loading ...; fig 6	Demacopulo B, Lema B, Cabrini R, Kreimann E. Similar expression pattern of NHERF1 and EZRIN in papillary but not in solid areas of human serous ovarian carcinomas. Acta Histochem. 2016;118:797-805 pubmed publisher
immunohistochemistry - paraffin section; human; 1:150; loading ...; fig 1d	Schirosi L, Mazzotta A, Opinto G, Pinto R, Graziano G, Tommasi S, et al. Î²-catenin interaction with NHERF1 and RASSF1A methylation in metastatic colorectal cancer patients. Oncotarget. 2016;7:67841-67850 pubmed publisher
	Wu C, Lidsky P, Xiao Y, Cheng R, Lee I, Nakayama T, et al. SARS-CoV-2 replication in airway epithelia requires motile cilia and microvillar reprogramming. Cell. 2023;186:112-130.e20 pubmed publisher
	Paredes J, Ji P, Lacomb J, Shroyer K, Martello L, Williams J. Establishment of three novel cell lines derived from African American patients with colorectal carcinoma: A unique tool for assessing racial health disparity. Int J Oncol. 2018;53:1516-1528 pubmed publisher
	Shankar A, Crouch D, Macluskey M. Ezrin-Radixin-Moesin Binding Phosphoprotein 50: A Potential Novel Biomarker in Human Papilloma Virus-Associated Head and Neck Squamous Cell Carcinomas. Head Neck Pathol. 2019;13:188-197 pubmed publisher
	Saponaro C, Vagheggini A, Scarpi E, Centonze M, Catacchio I, Popescu O, et al. NHERF1 and tumor microenvironment: a new scene in invasive breast carcinoma. J Exp Clin Cancer Res. 2018;37:96 pubmed publisher
	Opinto G, Silvestris N, Centonze M, Graziano G, Pinto R, Fucci L, et al. Hierarchical clustering analysis identifies metastatic colorectal cancers patients with more aggressive phenotype. Oncotarget. 2017;8:87782-87794 pubmed publisher
	Georgescu M, Mobley B, Orr B, Shang P, Lehman N, Zhu X, et al. NHERF1/EBP50 and NF2 as diagnostic markers for choroid plexus tumors. Acta Neuropathol Commun. 2016;4:55 pubmed publisher
	Abbattiscianni A, Favia M, Mancini M, Cardone R, Guerra L, Monterisi S, et al. Correctors of mutant CFTR enhance subcortical cAMP-PKA signaling through modulating ezrin phosphorylation and cytoskeleton organization. J Cell Sci. 2016;129:1128-40 pubmed publisher
	Schirosi L, De Summa S, Tommasi S, Paradiso A, Sambiasi D, Popescu O, et al. Immunoprofile from tissue microarrays to stratify familial breast cancer patients. Oncotarget. 2015;6:27865-79 pubmed publisher
	Mangia A, Caldarola L, Dell Endice S, Scarpi E, Saragoni L, Monti M, et al. The potential predictive role of nuclear NHERF1 expression in advanced gastric cancer patients treated with epirubicin/oxaliplatin/capecitabine first line chemotherapy. Cancer Biol Ther. 2015;16:1140-7 pubmed publisher
	Mangia A, Partipilo G, Schirosi L, Saponaro C, Galetta D, Catino A, et al. Fine Needle Aspiration Cytology: A Tool to Study NHERF1 Expression as a Potential Marker of Aggressiveness in Lung Cancer. Mol Biotechnol. 2015;57:549-57 pubmed publisher

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product information

Product Type :

Antibody

Product Name :

EBP50 Polyclonal Antibody

Catalog # :

PA1-090

Quantity :

100 µg

Price :

US 480

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Human, Mouse

Applications :

Immunocytochemistry: 2 µg/mL, Immunofluorescence: 2 µg/mL, Immunohistochemistry (Paraffin): 10 µg/mL, Immunoprecipitation: Assay dependent, Western Blot: 0.2-1 µg/mL

Species :

Human, Mouse

Isotype :

IgG

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

NHERF1 (Na+/H+ exchanger regulatory factor 1), also known as EBP50 (ezrin, radixin, moesin-binding phosphoprotein 50) is an adaptor protein, which associates with beta-catenin and is required for its localization at the cell-cell junctions, interacts with various G protein-coupled receptors and regulates their traffic, as well as sodium-hydrogen exchange and sodium-dependent phosphate transport. NHERF1/EBP50 inhibits cell motility and is required to suppress anchorage-independent growth. It contains C-terminal ERM (ezrin, radixin, moesin)-binding region and two N-terminal PDZ (postsynaptic-density-95/disc-large/ZO1 homology) domains and is able to form head-to-tail intramolecular conformation to regulate its interactions.

Immunogen :

Bacterially expressed, His-tagged human EBP50.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 2 µg/mL, Immunofluorescence: 2 µg/mL, Immunohistochemistry (Paraffin): 10 µg/mL, Immunoprecipitation: Assay dependent, Western Blot: 0.2-1 µg/mL

Aliases :

EBP50; EBP-50; ERM-binding phosphoprotein; ezrin-radixin-moesin binding phosphoprotein-50; ezrin-radixin-moesin-binding phosphoprotein 50; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; NHERF; NHE-RF; NHERF1; NHERF-1; NPHLOP2; Regulatory cofactor of Na(+)/H(+) exchanger; SLC9A3 regulator 1; SLC9A3R1; So; Sodium-hydrogen exchanger regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1; solute carrier family 9 isoform A3 regulatory factor 1; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1

more info or order :

Invitrogen product webpage