RAGE Polyclonal Antibody | Invitrogen PA1-075 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

RAGE Polyclonal Antibody

catalog :

PA1-075

quantity :

100 µg

price :

US 450

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; human; 1:100; loading ...; fig 3 western blot; human; loading ...; fig 6	Vetuschi A, Pompili S, Di Marco G, Calvaruso F, Iacomino E, Angelosante L, et al. Can the AGE/RAGE/ERK signalling pathway and the epithelial-to-mesenchymal transition interact in the pathogenesis of chronic rhinosinusitis with nasal polyps?. Eur J Histochem. 2020;64: pubmed publisher
	Zhang W, Zhao L, Zhang J, Li P, Lv Z. Metformin improves cognitive impairment in diabetic mice induced by a combination of streptozotocin and isoflurane anesthesia. Bioengineered. 2021;12:10982-10993 pubmed publisher
	Swami P, O Connell K, Thiyagarajan S, Crawford A, Patil P, Radhakrishnan P, et al. Inhibition of the Receptor for Advanced Glycation End Products Enhances the Cytotoxic Effect of Gemcitabine in Murine Pancreatic Tumors. Biomolecules. 2021;11: pubmed publisher
	Di Emidio G, Rea F, Placidi M, Rossi G, Cocciolone D, Virmani A, et al. Regulatory Functions of L-Carnitine, Acetyl, and Propionyl L-Carnitine in a PCOS Mouse Model: Focus on Antioxidant/Antiglycative Molecular Pathways in the Ovarian Microenvironment. Antioxidants (Basel). 2020;9: pubmed publisher
	Mastrocola R, Collotta D, Gaudioso G, Le Berre M, Cento A, Ferreira Alves G, et al. Effects of Exogenous Dietary Advanced Glycation End Products on the Cross-Talk Mechanisms Linking Microbiota to Metabolic Inflammation. Nutrients. 2020;12: pubmed publisher
	Emidio G, Placidi M, Rea F, Rossi G, Falone S, Cristiano L, et al. Methylglyoxal-Dependent Glycative Stress and Deregulation of SIRT1 Functional Network in the Ovary of PCOS Mice. Cells. 2020;9: pubmed publisher
	Vetuschi A, Pompili S, Gallone A, D Alfonso A, Carbone M, Carta G, et al. Immunolocalization of Advanced Glycation End Products, Mitogen Activated Protein Kinases, and Transforming Growth Factor-β/Smads in Pelvic Organ Prolapse. J Histochem Cytochem. 2018;66:673-686 pubmed publisher
	Kummarapurugu A, Zheng S, LEDFORD J, Karandashova S, Voynow J. High-Mobility Group Box 1 Upregulates MUC5AC and MUC5B Expression in Primary Airway Epithelial Cells. Am J Respir Cell Mol Biol. 2018;58:126-128 pubmed publisher

image

image 1 :

Immunofluorescent analysis of RAGE in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a RAGE polyclonal antibody (Product # PA1-075) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). RAGE staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

image 2 :

Immunohistochemistry was performed on normal biopsies of deparaffinized Mouse lymph node tissue . To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing RAGE (PA1-075) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

product information

Product Type :

Antibody

Product Name :

RAGE Polyclonal Antibody

Catalog # :

PA1-075

Quantity :

100 µg

Price :

US 450

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Mouse, Rat

Applications :

Immunohistochemistry (Frozen): 1-2 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1 µg/mL

Species :

Mouse, Rat

Isotype :

IgG

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Able to phosphorylate several exogenous substrates and to undergo autophosphorylation.

Immunogen :

Synthetic peptides corresponding to residues C(362) W R K R Q P R (R/L) E E R K A P E S Q E D(380) of rat RAGE.

Format :

Liquid

Applications w/Dilutions :

Immunohistochemistry (Frozen): 1-2 µg/mL, Immunohistochemistry (Paraffin): 1:10-1:100, Western Blot: 1 µg/mL

Aliases :

advanced glycation end product receptor; advanced glycation end-products receptor; advanced glycosylation end product-specific receptor; advanced glycosylation end product-specific receptor variant 2; advanced glycosylation end product-specific receptor variant 3; advanced glycosylation end product-specific receptor variant 4; advanced glycosylation end product-specific receptor variant 5; AGER; Rage; RAGE isoform NtRAGE-delta; RAGE isoform sRAGE-delta; RAGE/AGER; RAGE-4 ORF3; receptor for advanced glycation end-products variant 20; receptor for advanced glycosylation end products; SCARJ1; sRAGE

more info or order :

Invitrogen product webpage