Furin Polyclonal Antibody | Invitrogen PA1-062 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Furin Polyclonal Antibody

catalog :

PA1-062

quantity :

100 µL

price :

US 562

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

African green monkey, human, mouse, rat, dogs

application :

western blot, immunocytochemistry, immunoprecipitation, western blot knockout validation

more info or order :

Invitrogen product webpage

citations: 14

Published Application/Species/Sample/Dilution	Reference
western blot knockout validation; human; 1:500; loading ...; fig 4j, s5o	Vichas A, Riley A, Nkinsi N, Kamlapurkar S, Parrish P, Lo A, et al. Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer. Nat Commun. 2021;12:4789 pubmed publisher
western blot; African green monkey; 1:500; loading ...; fig 7c western blot; human; 1:500; loading ...; fig 9c western blot; rat; 1:500; fig 10	Kawahara K, Hirata H, Ohbuchi K, Nishi K, Maeda A, Kuniyasu A, et al. The novel monoclonal antibody 9F5 reveals expression of a fragment of GPNMB/osteoactivin processed by furin-like protease(s) in a subpopulation of microglia in neonatal rat brain. Glia. 2016;64:1938-61 pubmed publisher
immunocytochemistry; human; 1:100; fig 2	Yamamoto H, Ramos Molina B, Lick A, Prideaux M, Albornoz V, Bonewald L, et al. Posttranslational processing of FGF23 in osteocytes during the osteoblast to osteocyte transition. Bone. 2016;84:120-130 pubmed publisher
	Park H, Seo S, Sim J, Hwang S, Kim Y, Shin D, et al. TMED3 Complex Mediates ER Stress-Associated Secretion of CFTR, Pendrin, and SARS-CoV-2 Spike. Adv Sci (Weinh). 2022;:e2105320 pubmed publisher
	Tse L, Meganck R, Dong S, Adams L, White L, Mallory M, et al. Generation of Mature DENVs via Genetic Modification and Directed Evolution. MBio. 2022;13:e0038622 pubmed publisher
	Wu Y, Huang Y, Zhang W, Gunst S. The proprotein convertase furin inhibits IL-13-induced inflammation in airway smooth muscle by regulating integrin-associated signaling complexes. Am J Physiol Lung Cell Mol Physiol. 2021;321:L102-L115 pubmed publisher
	Yin J, Chang H, Yi Y, Yao Y, Leung P. TGF-β1 Increases GDNF Production by Upregulating the Expression of GDNF and Furin in Human Granulosa-Lutein Cells. Cells. 2020;9: pubmed publisher
	Lemaitre R, Bogdanova A, Borgonovo B, Woodruff J, Drechsel D. FlexiBAC: a versatile, open-source baculovirus vector system for protein expression, secretion, and proteolytic processing. BMC Biotechnol. 2019;19:20 pubmed publisher
	Shi M, Chen B, Mahajan D, Boh B, Zhou Y, Dutta B, et al. Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5. Nat Commun. 2018;9:4987 pubmed publisher
	Al Rifai O, Chow J, LaCombe J, Julien C, Faubert D, Susan Resiga D, et al. Proprotein convertase furin regulates osteocalcin and bone endocrine function. J Clin Invest. 2017;127:4104-4117 pubmed publisher
	Bronnimann M, Calton C, Chiquette S, Li S, Lu M, Chapman J, et al. Furin Cleavage of L2 during Papillomavirus Infection: Minimal Dependence on Cyclophilins. J Virol. 2016;90:6224-6234 pubmed publisher
	Chang H, Cheng J, Klausen C, Leung P. Recombinant BMP4 and BMP7 increase activin A production by up-regulating inhibin Î²A subunit and furin expression in human granulosa-lutein cells. J Clin Endocrinol Metab. 2015;100:E375-86 pubmed publisher
	FOLSCH H, Pypaert M, Maday S, Pelletier L, Mellman I. The AP-1A and AP-1B clathrin adaptor complexes define biochemically and functionally distinct membrane domains. J Cell Biol. 2003;163:351-62 pubmed
	FOLSCH H, Pypaert M, Schu P, Mellman I. Distribution and function of AP-1 clathrin adaptor complexes in polarized epithelial cells. J Cell Biol. 2001;152:595-606 pubmed

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product information

Product Type :

Antibody

Product Name :

Furin Polyclonal Antibody

Catalog # :

PA1-062

Quantity :

100 µL

Price :

US 562

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Canine, Human, Mouse

Applications :

Immunocytochemistry: 2 µg/mL, Immunofluorescence: 2 µg/mL, Immunoprecipitation: 1:100, Western Blot: 1:500-1:2000

Species :

Canine, Human, Mouse

Isotype :

IgG

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

Furin is a membrane-associated, calcium-dependent, serine protease that belongs to the subtilisin-like prohormone convertase (PC) family. Members of this family of cellular enzymes cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. Furin and other PC family members share structural similarities which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. Furin is the only known PC to possess a transmembrane domain. Cleavage of target proteins occurs at the carboxyl-terminus of the furin consensus sequence, RX(K/R)R. It has been shown that the acidic peptide sequence, C771PSDSEEDEG780, localizes furin to the trans-Golgi-network. Phosphorylation of serine residues within this region modulates intracellular routing of furin protein. An additional signaling domain includes the tetrapeptide sequence, Y759KGL762, which directs internalization from the cell surface.

Immunogen :

Synthetic peptide corresponding to residues R(780) G E R T A F I K D Q S A L(793) of human Furin.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 2 µg/mL, Immunofluorescence: 2 µg/mL, Immunoprecipitation: 1:100, Western Blot: 1:500-1:2000

Aliases :

9130404I01Rik; dibasic processing enzyme; dibasic-processing enzyme; FES upstream region; FUR; FURIN; furin (paired basic amino acid cleaving enzyme); furin, membrane associated receptor protein; furin, paired basic amino acid cleaving enzyme; PACE; Paired basic amino acid cleaving enzyme (furin); paired basic amino acid residue cleaving enzyme; paired basic amino acid residue-cleaving enzyme; Pcsk3; Prohormone convertase 3; proprotein convertase subtilisin/kexin type 3; Proprotein convertase subtilisin/kexin type 3 (paired basic amino acid cleaving enzyme, furin, membrane associated receptor protein); proprotein convertase subtilisin/kexin type3; SPC1; subtilisin pro-protein processing enzyme

more info or order :

Invitrogen product webpage