iNOS Polyclonal Antibody | Invitrogen PA1-036 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

iNOS Polyclonal Antibody

catalog :

PA1-036

quantity :

200 µL

price :

US 450

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 45

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; rat; 1:50; loading ...; fig 6e	Hannawi Y, Ewees M, Moore J, Zweier J. Characterizing CD38 Expression and Enzymatic Activity in the Brain of Spontaneously Hypertensive Stroke-Prone Rats. Front Pharmacol. 2022;13:881708 pubmed publisher
immunohistochemistry; mouse; 1:400; loading ...; fig 1h	He L, Bhat K, Duhacheck Muggy S, Ioannidis A, Zhang L, Nguyen N, et al. Tumor necrosis factor receptor signaling modulates carcinogenesis in a mouse model of breast cancer. Neoplasia. 2021;23:197-209 pubmed publisher
immunohistochemistry - paraffin section; rat; 1:200; loading ...; fig 2d	Amer M, Mohamed N, Shaalan A. Gestational protein restriction: study of the probable effects on cardiac muscle structure and function in adult rats. Histol Histopathol. 2017;32:1293-1303 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 5a	Menzel L, Kleber L, Friedrich C, Hummel R, Dangel L, Winter J, et al. Progranulin protects against exaggerated axonal injury and astrogliosis following traumatic brain injury. Glia. 2017;65:278-292 pubmed publisher
immunohistochemistry - paraffin section; rat; fig 3	TakÄ±r S, GÃ¼rel GÃ¼revin E, Toprak A, Demirci Tansel C, UydeÅŸ DoÄŸan B. The elevation of intraocular pressure is associated with apoptosis and increased immunoreactivity for nitric oxide synthase in rat retina whereas the effectiveness of retina derived relaxing factor is unaffected. Exp Eye Res. 2016;145:401-411 pubmed publisher
immunohistochemistry - paraffin section; human; 1 ug/ml; loading ...; fig s1	Soendergaard C, Kvist P, Seidelin J, Pelzer H, Nielsen O. Systemic and intestinal levels of factor XIII-A: the impact of inflammation on expression in macrophage subtypes. J Gastroenterol. 2016;51:796-807 pubmed publisher
	AlQot H, Rylett R. A novel transgenic mouse model expressing primate-specific nuclear choline acetyltransferase: insights into potential cholinergic vulnerability. Sci Rep. 2023;13:3037 pubmed publisher
	Wang L, Wang D, Sonzogni O, Ke S, Wang Q, Thavamani A, et al. PARP-inhibition reprograms macrophages toward an anti-tumor phenotype. Cell Rep. 2022;41:111462 pubmed publisher
	He Y, Yao M, Zhou J, Xie J, Liang C, Yin D, et al. Mg(OH)2 nanosheets on Ti with immunomodulatory function for orthopedic applications. Regen Biomater. 2022;9:rbac027 pubmed publisher
	Chen X, Li C, Cao X, Jia X, Chen X, Wang Z, et al. Mitochondria-targeted supramolecular coordination container encapsulated with exogenous itaconate for synergistic therapy of joint inflammation. Theranostics. 2022;12:3251-3272 pubmed publisher
	Xue M, Sheng W, Song X, Shi Y, Geng Z, Shen L, et al. Atractylenolide III ameliorates spinal cord injury in rats by modulating microglial/macrophage polarization. CNS Neurosci Ther. 2022;28:1059-1071 pubmed publisher
	Ding X, Zhou J, Zhao L, Chen M, Wang S, Zhang M, et al. Intestinal Flora Composition Determines Microglia Activation and Improves Epileptic Episode Progress. Front Cell Infect Microbiol. 2022;12:835217 pubmed publisher
	Huang X, Yang Q, Xie L, Lei S. Histone methyltransferase enhancer of zeste 2 polycomb repressive complex 2 subunit exacerbates inflammation in depression rats by modulating microglia polarization. Bioengineered. 2022;13:5509-5524 pubmed publisher
	Park G, Tarafder S, Eyen S, Park S, Kim R, Siddiqui Z, et al. Oxo-M and 4-PPBP Delivery via Multi-Domain Peptide Hydrogel Toward Tendon Regeneration. Front Bioeng Biotechnol. 2022;10:773004 pubmed publisher
	Zhang P, Ohkawa Y, Yamamoto S, Momota H, Kato A, Kaneko K, et al. St8sia1-deficiency in mice alters tumor environments of gliomas, leading to reduced disease severity. Nagoya J Med Sci. 2021;83:535-549 pubmed publisher
	Davis T, Conradie D, Shridas P, de Beer F, Engelbrecht A, de Villiers W. Serum Amyloid A Promotes Inflammation-Associated Damage and Tumorigenesis in a Mouse Model of Colitis-Associated Cancer. Cell Mol Gastroenterol Hepatol. 2021;12:1329-1341 pubmed publisher
	Li M, McGhee E, Shinno L, Lee K, Lin Y. Exposure to Microbial Metabolite Butyrate Prolongs the Survival Time and Changes the Growth Pattern of Human Papillomavirus 16 E6/E7-Immortalized Keratinocytes in Vivo. Am J Pathol. 2021;191:1822-1836 pubmed publisher
	Tran V, Kang Y, Kim H, Kim H, Cho H. Oral Pathogenic Bacteria-Inducing Neurodegenerative Microgliosis in Human Neural Cell Platform. Int J Mol Sci. 2021;22: pubmed publisher
	Eitner A, M xfc ller S, K xf6 nig C, Wilharm A, Raab R, Hofmann G, et al. Inhibition of Inducible Nitric Oxide Synthase Prevents IL-1β-Induced Mitochondrial Dysfunction in Human Chondrocytes. Int J Mol Sci. 2021;22: pubmed publisher
	Varinthra P, Ganesan K, Huang S, Chompoopong S, Eurtivong C, Suresh P, et al. The 4-(Phenylsulfanyl) butan-2-one Improves Impaired Fear Memory Retrieval and Reduces Excessive Inflammatory Response in Triple Transgenic Alzheimer's Disease Mice. Front Aging Neurosci. 2021;13:615079 pubmed publisher
	Nabil M, El Raey M, Abdo W, Abdelfattah M, El Shazly A, Sobeh M, et al. Gastro-Protective Effects of Albizia anthelmintica Leaf Extract on Indomethacin-Induced Gastric Ulcer in Wistar Rats: In Silico and In Vivo Studies. Antioxidants (Basel). 2021;10: pubmed publisher
	Cheng S, Zhang D, Li M, Liu X, Zhang Y, Qian S, et al. Osteogenesis, angiogenesis and immune response of Mg-Al layered double hydroxide coating on pure Mg. Bioact Mater. 2021;6:91-105 pubmed publisher
	Na L, Wang S, Liu T, Zhang L. Ultrashort Wave Combined with Human Umbilical Cord Mesenchymal Stem Cell (HUC-MSC) Transplantation Inhibits NLRP3 Inflammasome and Improves Spinal Cord Injury via MK2/TTP Signalling Pathway. Biomed Res Int. 2020;2020:3021750 pubmed publisher
	Battaglia C, Cursano S, Calzia E, Catanese A, Boeckers T. Corticotropin-releasing hormone (CRH) alters mitochondrial morphology and function by activating the NF-kB-DRP1 axis in hippocampal neurons. Cell Death Dis. 2020;11:1004 pubmed publisher
	Delage C, Cornil C. Estrogen-dependent sex difference in microglia in the developing brain of Japanese quail (Coturnix japonica). Dev Neurobiol. 2020;80:239-262 pubmed publisher
	Yang P, Wang X, Wang D, Shi Y, Zhang M, Yu T, et al. Topical insulin application accelerates diabetic wound healing by promoting anti-inflammatory macrophage polarization. J Cell Sci. 2020;133: pubmed publisher
	Lee J, Jeon Y, Kang J, Lee S, Lee H, Son C. Aquilariae Lignum Methylene Chloride Fraction Attenuates IL-1β-Driven Neuroinflammation in BV2 Microglial Cells. Int J Mol Sci. 2020;21: pubmed publisher
	Mariod A, Salama S. The Efficacy of Processing Strategies on the Gastroprotective Potentiality of Chenopodium quinoa Seeds. ScientificWorldJournal. 2020;2020:6326452 pubmed publisher
	Wang T, Jia Q, Chen T, Yin H, Tian X, Lin X, et al. Alleviation of Synovial Inflammation of Juanbi-Tang on Collagen-Induced Arthritis and TNF-Tg Mice Model. Front Pharmacol. 2020;11:45 pubmed publisher
	Zheng X, Fernando V, Sharma V, Walia Y, Letson J, Furuta S. Correction of arginine metabolism with sepiapterin-the precursor of nitric oxide synthase cofactor BH4-induces immunostimulatory-shift of breast cancer. Biochem Pharmacol. 2020;176:113887 pubmed publisher
	Zhang X, Li J, Luo S, Wang M, Huang Q, Deng Z, et al. IgE Contributes to Atherosclerosis and Obesity by Affecting Macrophage Polarization, Macrophage Protein Network, and Foam Cell Formation. Arterioscler Thromb Vasc Biol. 2020;40:597-610 pubmed publisher
	Jiménez Altayó F, Ortiz Romero P, Puertas Umbert L, Dantas A, Perez B, Vila E, et al. Stenosis coexists with compromised α1-adrenergic contractions in the ascending aorta of a mouse model of Williams-Beuren syndrome. Sci Rep. 2020;10:889 pubmed publisher
	Di Gioia M, Spreafico R, Springstead J, Mendelson M, Joehanes R, Levy D, et al. Endogenous oxidized phospholipids reprogram cellular metabolism and boost hyperinflammation. Nat Immunol. 2020;21:42-53 pubmed publisher
	Lima Cabello E, Alché J, Morales Santana S, Clemente A, Jimenez Lopez J. Narrow-Leafed Lupin (Lupinus angustifolius L.) Seeds Gamma-Conglutin is an Anti-Inflammatory Protein Promoting Insulin Resistance Improvement and Oxidative Stress Amelioration in PANC-1 Pancreatic Cell-Line. Antioxidants (Basel). 2019;9: pubmed publisher
	Wang C, Zhang L, Ndong J, Hettinghouse A, Sun G, Chen C, et al. Progranulin deficiency exacerbates spinal cord injury by promoting neuroinflammation and cell apoptosis in mice. J Neuroinflammation. 2019;16:238 pubmed publisher
	Giménez Gómez P, Pérez Hernández M, O Shea E, Caso J, Martín Hernández D, Cervera L, et al. Changes in brain kynurenine levels via gut microbiota and gut-barrier disruption induced by chronic ethanol exposure in mice. FASEB J. 2019;33:12900-12914 pubmed publisher
	Wang H, Fu L, Cheng C, Gao R, Lin M, Su H, et al. Inhibition of LPS-Induced Oxidative Damages and Potential Anti-Inflammatory Effects of Phyllanthus emblica Extract via Down-Regulating NF-κB, COX-2, and iNOS in RAW 264.7 Cells. Antioxidants (Basel). 2019;8: pubmed publisher
	Grancieri M, Martino H, Gonzalez de Mejia E. Chia (Salvia hispanica L.) Seed Total Protein and Protein Fractions Digests Reduce Biomarkers of Inflammation and Atherosclerosis in Macrophages In Vitro. Mol Nutr Food Res. 2019;63:e1900021 pubmed publisher
	Song J, Kang H, Ju H, Park A, Park H, Hong J, et al. Umbilical cord-derived mesenchymal stem cell extracts ameliorate atopic dermatitis in mice by reducing the T cell responses. Sci Rep. 2019;9:6623 pubmed publisher
	Cheng Z, Yang Y, Duan F, Lou B, Zeng J, Huang Y, et al. Inhibition of Notch1 Signaling Alleviates Endotoxin-Induced Inflammation Through Modulating Retinal Microglia Polarization. Front Immunol. 2019;10:389 pubmed publisher
	Lau W, Khazaeli M, Savoj J, Manekia K, Bangash M, Thakurta R, et al. Dietary tetrahydrocurcumin reduces renal fibrosis and cardiac hypertrophy in 5/6 nephrectomized rats. Pharmacol Res Perspect. 2018;6:e00385 pubmed publisher
	Bwalya E, Kim S, Fang J, Wijekoon H, Hosoya K, Okumura M. Pentosan polysulfate inhibits IL-1?-induced iNOS, c-Jun and HIF-1? upregulation in canine articular chondrocytes. PLoS ONE. 2017;12:e0177144 pubmed publisher
	Asano S, Arvapalli R, Manne N, Maheshwari M, Ma B, Rice K, et al. Cerium oxide nanoparticle treatment ameliorates peritonitis-induced diaphragm dysfunction. Int J Nanomedicine. 2015;10:6215-25 pubmed publisher
	Manrique W, Claudiano G, de Castro M, Petrillo T, Pereira Figueiredo M, Belo M, et al. Expression of cellular components in granulomatous inflammatory response in Piaractus mesopotamicus model. PLoS ONE. 2015;10:e0121625 pubmed publisher
	Lau W, Liu S, Pahlevan S, Yuan J, Khazaeli M, Ni Z, et al. Role of Nrf2 dysfunction in uremia-associated intestinal inflammation and epithelial barrier disruption. Dig Dis Sci. 2015;60:1215-22 pubmed publisher

image

image 1 :

Immunofluorescent analysis of iNOS in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a iNOS polyclonal antibody (Product # PA1-036) at a dilution of 1:20 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). iNOS staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

product information

Product Type :

Antibody

Product Name :

iNOS Polyclonal Antibody

Catalog # :

PA1-036

Quantity :

200 µL

Price :

US 450

Clonality :

Polyclonal

Purity :

Ammonium sulfate precipitation

Host :

Rabbit

Reactivity :

Human, Mouse, Rat

Applications :

Immunocytochemistry: 1:50, Immunofluorescence: 1:50, Immunohistochemistry: 1:20, Western Blot: 1:200-1:1,000

Species :

Human, Mouse, Rat

Isotype :

IgG

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

iNOS (Inducible Nitric oxide, NO, NOS) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. iNOS is expressed in liver and inducible by a combination of lipopolysaccharide and certain cytokines. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Three related iNOS pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. Diseases associated with iNOS dysfunction include achalasia and impotence.

Immunogen :

Synthetic peptide corresponding to residues D(17) L K E E K D I N N N V K K T(31) of mouse iNOS.

Format :

Liquid

Applications w/Dilutions :

Immunocytochemistry: 1:50, Immunofluorescence: 1:50, Immunohistochemistry: 1:20, Western Blot: 1:200-1:1,000

Aliases :

Hepatocyte NOS; hepatocytes; HEP-NOS; inducible nitric oxide synthase; Inducible NO synthase; inducible NOS; iNos; i-NOS; Inosl; MAC-NOS; macrophage NOS; nitric oxide synthase 2; nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase, inducible; nitric oxide synthase, macrophage; nitric oxide synthase-inducible; NOS; NOS type II; NOS, type II; NOS2; Nos-2; NOS2A; NOS-II; OTTMUSP00000000202; Peptidyl-cysteine S-nitrosylase NOS2

more info or order :

Invitrogen product webpage