GRP78 Polyclonal Antibody | Invitrogen PA1-014A product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

GRP78 Polyclonal Antibody

catalog :

PA1-014A

quantity :

100 µg

price :

US 500

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dogs

application :

western blot, immunocytochemistry

more info or order :

Invitrogen product webpage

citations: 17

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:2000; loading ...; fig 7h	Santos M, Anderson C, Neschen S, Zumbrennen Bullough K, Romney S, Kahle Stephan M, et al. Irp2 regulates insulin production through iron-mediated Cdkal1-catalyzed tRNA modification. Nat Commun. 2020;11:296 pubmed publisher
	Pan J, Sun D, Lee D, Xiong L, Ren X, Guo H, et al. Osteoblastic Swedish mutant APP expedites brain deficits by inducing endoplasmic reticulum stress-driven senescence. Commun Biol. 2021;4:1326 pubmed publisher
	George G, Ninagawa S, Yagi H, Furukawa J, Hashii N, Ishii Watabe A, et al. Purified EDEM3 or EDEM1 alone produces determinant oligosaccharide structures from M8B in mammalian glycoprotein ERAD. elife. 2021;10: pubmed publisher
	Tsai S, Atai N, Cacciottolo M, Nice J, Salehi A, Guo C, et al. Exosome-mediated mRNA delivery in vivo is safe and can be used to induce SARS-CoV-2 immunity. J Biol Chem. 2021;297:101266 pubmed publisher
	Toldi V, Kassay N, Szab xf3 A. Missense PNLIP mutations impeding pancreatic lipase secretion cause protein misfolding and endoplasmic reticulum stress. Pancreatology. 2021;21:1317-1325 pubmed publisher
	Karunarathne W, Molagoda I, Lee K, Choi Y, Yu S, Kang C, et al. Protective Effect of Anthocyanin-Enriched Polyphenols from Hibiscus syriacus L. (Malvaceae) against Ultraviolet B-Induced Damage. Antioxidants (Basel). 2021;10: pubmed publisher
	Liao C, Tzeng Y, Lai G, Chang C, Hu M, Tsai W, et al. Omega-3 Fatty Acid-Enriched Fish Oil and Selenium Combination Modulates Endoplasmic Reticulum Stress Response Elements and Reverses Acquired Gefitinib Resistance in HCC827 Lung Adenocarcinoma Cells. Mar Drugs. 2020;18: pubmed publisher
	Li X, Yang L, Mao Z, Pan X, Zhao Y, Gu X, et al. Novel role of dynamin-related-protein 1 in dynamics of ER-lipid droplets in adipose tissue. FASEB J. 2020;34:8265-8282 pubmed publisher
	Fisher J, McKeating D, Cuffe J, Bianco Miotto T, Holland O, Perkins A. Proteomic Analysis of Placental Mitochondria Following Trophoblast Differentiation. Front Physiol. 2019;10:1536 pubmed publisher
	Seo S, Kang S, Kwon O, Bang S, Kim S, Choi K, et al. A mechanical acupuncture instrument mitigates the endoplasmic reticulum stress and oxidative stress of ovariectomized rats. Integr Med Res. 2019;8:187-194 pubmed publisher
	Lenin R, Nagy P, Jha K, GANGARAJU R. GRP78 translocation to the cell surface and O-GlcNAcylation of VE-Cadherin contribute to ER stress-mediated endothelial permeability. Sci Rep. 2019;9:10783 pubmed publisher
	Kao R, Lai G, Chow J, Liao C, Zheng Y, Tsai W, et al. Opposite Regulation of CHOP and GRP78 and Synergistic Apoptosis Induction by Selenium Yeast and Fish Oil via AMPK Activation in Lung Adenocarcinoma Cells. Nutrients. 2018;10: pubmed publisher
	Lenin R, Nagy P, Gentry J, GANGARAJU R. Featured Article: Deterioration of visual function mediated by senescence-associated endoplasmic reticulum stress in inflammatory tie2-TNF mice. Exp Biol Med (Maywood). 2018;243:976-984 pubmed publisher
	Dolai S, Liang T, Orabi A, Xie L, Holmyard D, Javed T, et al. Depletion of the membrane-fusion regulator Munc18c attenuates caerulein hyperstimulation-induced pancreatitis. J Biol Chem. 2018;293:2510-2522 pubmed publisher
	Gupta A, Hossain M, Miller N, Kerin M, Callagy G, Gupta S. NCOA3 coactivator is a transcriptional target of XBP1 and regulates PERK-eIF2Î±-ATF4 signalling in breast cancer. Oncogene. 2016;35:5860-5871 pubmed publisher
	Gupta A, Hossain M, Read D, Hetz C, Samali A, Gupta S. PERK regulated miR-424(322)-503 cluster fine-tunes activation of IRE1 and ATF6 during Unfolded Protein Response. Sci Rep. 2015;5:18304 pubmed publisher
	Gaide Chevronnay H, Janssens V, Van Der Smissen P, Liao X, Abid Y, Nevo N, et al. A mouse model suggests two mechanisms for thyroid alterations in infantile cystinosis: decreased thyroglobulin synthesis due to endoplasmic reticulum stress/unfolded protein response and impaired lysosomal processing. Endocrinology. 2015;156:2349-64 pubmed publisher

image

image 1 :

Immunofluorescent analysis of Glucose Regulated Protein 78 using anti-Glucose Regulated Protein 78 polyclonal antibody (Product# PA1-014A) shows staining in HMVEC Cells.

image 2 :

Immunofluorescent analysis of Glucose Regulated Protein 78 using anti-Glucose Regulated Protein 78 polyclonal antibody (Product# PA1-014A) shows staining in p19 Cells.

image 3 :

product information

Product Type :

Antibody

Product Name :

GRP78 Polyclonal Antibody

Catalog # :

PA1-014A

Quantity :

100 µg

Price :

US 500

Clonality :

Polyclonal

Purity :

Antigen affinity chromatography

Host :

Rabbit

Reactivity :

Canine, Human, Mouse, Rat

Applications :

Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Western Blot: 1-2 µg/mL

Species :

Canine, Human, Mouse, Rat

Isotype :

IgG

Storage :

-20° C, Avoid Freeze/Thaw Cycles

Description :

GRP78 is a 78 kDa glucose regulated protein that belongs to the family of heat shock proteins that include HSP70. GRP78 is a resident protein of the endoplasmic reticulum (ER) and associates transiently with a variety of newly synthesized secretory and membrane proteins. GRP78 may also interact with mutant and misfolded proteins, marking them for degradation. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP94) and protein disulfide isomerase (PDI). The KDEL signal is recognized by the KDEL receptor and this interaction ensures recycling of escaped ER proteins back to the ER. GRP78’s involvement in correct folding of proteins and degradation of misfolded proteins is via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. GRP78 is critical for maintenance of cell homeostasis and the prevention of apoptosis. GRP78 protein levels have been shown to be a reliable biomarker of hypoglycemia and serves a neuroprotective function in neurons exposed to glutamate and oxidative stress. GRP78 levels are reduced in the brains of Alzheimer's Disease patients, and decreased expression of GRP78 is found associated with missense mutations in the human presenilin-1 (PS1) gene. Further, the induction of the GRP78 protein has been associated with the development of drug-resistance to antitumor drugs.

Immunogen :

Synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP78.

Format :

Lyophilized

Applications w/Dilutions :

Immunocytochemistry: 5 µg/mL, Immunofluorescence: 5 µg/mL, Western Blot: 1-2 µg/mL

Aliases :

78 kDa glucose-regulated protein; 78 kDa glucose-regulated protein homolog; AL022860; AU019543; baffled; Binding-immunoglobulin protein; biP; BiP protein; BiP/Grp78; cb865; D2Wsu141e; D2Wsu17e; Endoplasmic reticulum chaperone BiP; endoplasmic reticulum chaperone BiP; 78 kDa glucose-regulated protein; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; epididymis secretory sperm binding protein Li 89n; fb60h09; fi36d04; FLJ26106; glucose regulated protein, 78 kDa; glucose-regulated protein; glucose-regulated protein, 78kDa; GRP 78; GRP78; GRP-78; heat shock 70 kDa protein 5; heat shock 70 kDa protein 5a; heat shock 70kD protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5; heat shock 70kDa protein 5 (glucose-regulated protein); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); heat shock protein 5; heat shock protein 70 family protein 5; heat shock protein family A (Hsp70) member 5; heat shock protein family A (Hsp70) member 5 L homeolog; heat shock protein family A member 5; heavy-chain binding protein BiP; HEL-S-89n; Hsce70; hsp70; Hsp70 family ATPase KAR2; HSP70 family protein 5; HSPA 5; HSPA5; hspa5.L; hspa5a; I79_019946; immunoglobulin binding protein; immunoglobulin heavy chain-binding protein; Immunoglobulin heavy chain-binding protein homolog; J1248; KAR2; LOC100008764; mBiP; MIF2; Sez7; SEZ-7; SSD1; Steroidogenesis-activator polypeptide; wu:fb60h09; wu:fi36d04; XAP-1; XAP-1 antigen; XELAEV_180384511mg; YJL034W; zgc:55994; zgc:77606

more info or order :

Invitrogen product webpage