Penta-His Tag Monoclonal Antibody | Invitrogen P-21315 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Penta-His Tag Monoclonal Antibody

catalog :

P-21315

quantity :

100 ug

price :

US 954.00

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

application :

western blot, immunohistochemistry, immunocytochemistry, other

citations: 8

Reference
Pol xe1 chov xe1 E, Bach K, Heuten E, Stanchev S, Tich xe1 A, Lampe P, et al. Chemical Blockage of the Mitochondrial Rhomboid Protease PARL by Novel Ketoamide Inhibitors Reveals Its Role in PINK1/Parkin-Dependent Mitophagy. J Med Chem. 2023;66:251-265 pubmed publisher
Noor S, Hoffmann M, Rinis N, Bartels M, Winterhalter P, Hoelscher C, et al. Glycosyltransferase POMGNT1 deficiency strengthens N-cadherin-mediated cell-cell adhesion. J Biol Chem. 2021;296:100433 pubmed publisher
Fabre L, Ntreh A, Yazidi A, Leus I, Weeks J, Bhattacharyya S, et al. A "Drug Sweeping" State of the TriABC Triclosan Efflux Pump from Pseudomonas aeruginosa. Structure. 2021;29:261-274.e6 pubmed publisher
Reinhardt A, Wehle M, Geissner A, Crouch E, Kang Y, Yang Y, et al. Structure binding relationship of human surfactant protein D and various lipopolysaccharide inner core structures. J Struct Biol. 2016;195:387-395 pubmed publisher
Khakhum N, Yordpratum U, Boonmee A, Tattawasart U, Rodrigues J, Sermswan R. Identification of the Burkholderia pseudomallei bacteriophage ST79 lysis gene cassette. J Appl Microbiol. 2016;121:364-72 pubmed publisher
King M, Phillips G, Bignone P, Hayes N, Pinder J, Baines A. A conserved sequence in calmodulin regulated spectrin-associated protein 1 links its interaction with spectrin and calmodulin to neurite outgrowth. J Neurochem. 2014;128:391-402 pubmed publisher
Piercey Åkesson C, Press C, Tranulis M, Jeffrey M, Aleksandersen M, Landsverk T, et al. Phenotypic characterization of cells participating in transport of prion protein aggregates across the intestinal mucosa of sheep. Prion. 2012;6:261-75 pubmed publisher
Felle M, Joppien S, Nemeth A, Diermeier S, Thalhammer V, Dobner T, et al. The USP7/Dnmt1 complex stimulates the DNA methylation activity of Dnmt1 and regulates the stability of UHRF1. Nucleic Acids Res. 2011;39:8355-65 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Penta-His Tag Monoclonal Antibody

Catalog # :

P-21315

Quantity :

100 ug

Price :

US 954.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Tag

Applications :

Immunocytochemistry: 1:20-1:50, Immunohistochemistry: 1:20-1:50, Western Blot: 0.1-0.2 ug/mL

Species :

Tag

Isotype :

IgG1

Storage :

4 C

Description :

The oligohistidine domain is a Ni2+-binding peptide sequence comprising a string of four to six histidine residues. When the DNA sequence corresponding to the oligohistidine domain is fused in frame with a gene of interest, the resulting fusion protein can be easily purified using a nickel-chelating resin. The Penta-His monoclonal IgG1 antibody provides a sensitive method for specific detection of fusion proteins that have an oligohistidine domain comprising five or six consecutive histidine residues. The antibody does not recognize tetrahistidine domains or domains in which the histidine string is interrupted by another amino acid. The antibody binds to the oligohistidine domain regardless of the surrounding amino acid context and even when the group is partially hidden, although subtle differences in the amino acid context may change the sensitivity limit for a particular fusion protein. This antibody can be used to detect oligohistidine fusion proteins on western blots, dot blots or colony blots, or in tissues.

Immunogen :

Histidine Tag (5xHis)

Format :

Lyophilized

Applications w/Dilutions :

Immunocytochemistry: 1:20-1:50, Immunohistochemistry: 1:20-1:50, Western Blot: 0.1-0.2 ug/mL

Aliases :

5Xhis; Histidine 5X; pentahistidine