Amyloid Precursor Protein Monoclonal Antibody (mAbP2-1) | Invitrogen OMA1-03132 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Amyloid Precursor Protein Monoclonal Antibody (mAbP2-1)

catalog :

OMA1-03132

quantity :

50µg

price :

US 390

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

mAbP2-1

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry, immunoprecipitation

more info or order :

Invitrogen product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig st1	Klementieva O, Willén K, Martinsson I, Israelsson B, Engdahl A, Cladera J, et al. Pre-plaque conformational changes in Alzheimer's disease-linked Aβ and APP. Nat Commun. 2017;8:14726 pubmed publisher
ELISA; human	Butler C, Ogilvie K, Martinez Alsina L, Barreiro G, Beck E, Nolan C, et al. Aminomethyl-Derived Beta Secretase (BACE1) Inhibitors: Engaging Gly230 without an Anilide Functionality. J Med Chem. 2017;60:386-402 pubmed publisher
ELISA; human	Zuhl A, Nolan C, Brodney M, Niessen S, Atchison K, Houle C, et al. Chemoproteomic profiling reveals that cathepsin D off-target activity drives ocular toxicity of ?-secretase inhibitors. Nat Commun. 2016;7:13042 pubmed publisher
ELISA; human; fig 1	Brodney M, Beck E, Butler C, Barreiro G, Johnson E, Riddell D, et al. Utilizing structures of CYP2D6 and BACE1 complexes to reduce risk of drug-drug interactions with a novel series of centrally efficacious BACE1 inhibitors. J Med Chem. 2015;58:3223-52 pubmed publisher
ELISA; human; tbl 1	Butler C, Brodney M, Beck E, Barreiro G, Nolan C, Pan F, et al. Discovery of a series of efficient, centrally efficacious BACE1 inhibitors through structure-based drug design. J Med Chem. 2015;58:2678-702 pubmed publisher
ELISA; human	Brodney M, Barreiro G, Ogilvie K, Hajos Korcsok E, Murray J, Vajdos F, et al. Spirocyclic sulfamides as ?-secretase 1 (BACE-1) inhibitors for the treatment of Alzheimer's disease: utilization of structure based drug design, WaterMap, and CNS penetration studies to identify centrally efficacious inhibitors. J Med Chem. 2012;55:9224-39 pubmed publisher
immunoprecipitation; human	Portelius E, Brinkmalm G, Tran A, Andreasson U, Zetterberg H, Westman Brinkmalm A, et al. Identification of novel N-terminal fragments of amyloid precursor protein in cerebrospinal fluid. Exp Neurol. 2010;223:351-8 pubmed publisher
ELISA; human; 0.25 ug/ml	King G, Perez R, Steinhilb M, Gaut J, Turner R. X11alpha modulates secretory and endocytic trafficking and metabolism of amyloid precursor protein: mutational analysis of the YENPTY sequence. Neuroscience. 2003;120:143-54 pubmed
	Willén K, Edgar J, Hasegawa T, Tanaka N, Futter C, Gouras G. A? accumulation causes MVB enlargement and is modelled by dominant negative VPS4A. Mol Neurodegener. 2017;12:61 pubmed publisher
	He P, Li P, Hua Q, Liu Y, Staufenbiel M, Li R, et al. Chronic administration of anti-stroke herbal medicine TongLuoJiuNao reduces amyloidogenic processing of amyloid precursor protein in a mouse model of Alzheimer's disease. PLoS ONE. 2013;8:e58181 pubmed publisher
	Muresan Z, Muresan V. Coordinated transport of phosphorylated amyloid-beta precursor protein and c-Jun NH2-terminal kinase-interacting protein-1. J Cell Biol. 2005;171:615-25 pubmed

product information

Product Type :

Antibody

Product Name :

Amyloid Precursor Protein Monoclonal Antibody (mAbP2-1)

Catalog # :

OMA1-03132

Quantity :

50µg

Price :

US 390

Clonality :

Monoclonal

Purity :

Protein G

Host :

Mouse

Reactivity :

Human, Non-human primate

Applications :

ELISA: 10 µg/mL, Immunocytochemistry: 1:200, Immunofluorescence: 1:200, Immunoprecipitation: 25 µg/mL*, Western Blot: 5 µg/mL

Species :

Human, Non-human primate

Clone :

mAbP2-1

Isotype :

IgG1

Storage :

-80° C, Avoid Freeze/Thaw Cycles

Description :

Amyloid beta peptide (Abeta/Beta-amyloid) is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer's disease. Abeta peptide is 40-43 amino acids long and generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease, beta-secretase (BACE) which is synthesized as a propeptide and must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP, and a membrane-bound remnant. The remnant protein is processed by another protease, gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. The transmembrane glycoprotein, nicastrin, is associated with presinilins and has been found to bind to the carboxyl-terminus of beta APP and helps to modulate the production of the amyloid beta peptide. Abeta is an extracellular filamentous protein component of amyloid cores, neuritic plaques and is also found as a deposit in neurofibrillary tangles. Alzheimer’s disease, the most common cause of senile dementia, is characterized by abnormal filamentous protein deposits in the brain. Beta amyloid deposits are also detected in Lewy body dementia, Down’s syndrome, amyloidosis (Dutch type), cerebroarterial amyloidosis (cerebral amyloid angiopathy) and in the Guam Parkinson-Dementia complex.

Immunogen :

Native, secreted form of APP

Format :

Liquid

Applications w/Dilutions :

ELISA: 10 µg/mL, Immunocytochemistry: 1:200, Immunofluorescence: 1:200, Immunoprecipitation: 25 µg/mL*, Western Blot: 5 µg/mL

Aliases :

A4; AAA; ABETA; Abeta40; Abeta42; ABPP; AD1; Adap; AG; AICD-50; AICD-57; AICD-59; AID(50); AID(57); AID(59); Alpha-CTF; Alpha-secretase C-terminal fragment; Alzheimer disease; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; Amyloid; amyloid A4; Amyloid b; Amyloid beta; amyloid beta (A4) precursor protein; amyloid beta (A4) precursor protein (peptidase nexin-II, Alzheimer disease); amyloid beta A4 protein; amyloid beta precursor protein; Amyloid intracellular domain 50; Amyloid intracellular domain 57; Amyloid intracellular domain 59; Amyloid precursor protein; Amyloid ß; Amyloid-beta A4 protein; Amyloid-beta precursor protein; amyloid-beta protein 40; Amyloid-beta protein 42; Amyloidogenic glycoprotein; APP; APP-C57; APP-C59; APP-C99; APPI; appican; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); beta-amyloid precursor protein; betaApp; Beta-APP40; Beta-APP42; Beta-CTF; Beta-secretase C-terminal fragment; C31; C80; C83; C99; Cerebral vascular amyloid peptide; CTF gamma; CTF-alpha; CTFgamma; CVAP; E030013M08Rik; Gamma-CTF(50); Gamma-CTF(57); Gamma-CTF(59); Gamma-secretase C-terminal fragment 50; Gamma-secretase C-terminal fragment 57; Gamma-secretase C-terminal fragment 59; N-APP; OTTHUMP00000096096; P3(40); P3(42); Pan-Abeta; peptidase nexin-II; PN2; PN-II; PreA4; PreA4 751; protease nexin II; Protease nexin-II; S-APP-alpha; S-APP-beta; Soluble APP-alpha; Soluble APP-beta; testicular tissue protein Li 2

more info or order :

Invitrogen product webpage