This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
secondary antibody
product name :
F(ab')2-Goat anti-Rabbit IgG (H+L) Secondary Antibody, NANOGOLD
catalog :
N-24916
quantity :
1 mL
price :
US 644.00
citations: 87
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Mironov A, Beznoussenko G, Nicoziani P, Martella O, Trucco A, Kweon H, et al. Small cargo proteins and large aggregates can traverse the Golgi by a common mechanism without leaving the lumen of cisternae. J Cell Biol. 2001;155:1225-38 pubmed
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Sugita S, Han W, Butz S, Liu X, Fernandez Chacon R, Lao Y, et al. Synaptotagmin VII as a plasma membrane Ca(2+) sensor in exocytosis. Neuron. 2001;30:459-73 pubmed
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Bendayan M. Tech.Sight. Worth its weight in gold. Science. 2001;291:1363-5 pubmed
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Weipoltshammer K, Schöfer C, Almeder M, Wachtler F. Signal enhancement at the electron microscopic level using Nanogold and gold-based autometallography. Histochem Cell Biol. 2000;114:489-95 pubmed
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Graf A, Cheung A, Hauser Kornberger C, Dandachi N, Tubbs R, Dietze O, et al. Clinical relevance of HPV 16/18 testing methods in cervical squamous cell carcinoma. Appl Immunohistochem Mol Morphol. 2000;8:300-9 pubmed
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Yang R, Tabata S, Crowley H, Margolskee R, Kinnamon J. Ultrastructural localization of gustducin immunoreactivity in microvilli of type II taste cells in the rat. J Comp Neurol. 2000;425:139-51 pubmed
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Kohler A, Lauritzen B, Van Noorden C. Signal amplification in immunohistochemistry at the light microscopic level using biotinylated tyramide and nanogold-silver staining. J Histochem Cytochem. 2000;48:933-41 pubmed
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Bergles D, Roberts J, Somogyi P, Jahr C. Glutamatergic synapses on oligodendrocyte precursor cells in the hippocampus. Nature. 2000;405:187-91 pubmed
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Feng D, Nagy J, Brekken R, Pettersson A, Manseau E, Pyne K, et al. Ultrastructural localization of the vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) receptor-2 (FLK-1, KDR) in normal mouse kidney and in the hyperpermeable vessels induced by VPF/VEGF-expressing tumors and adenoviral vecto. J Histochem Cytochem. 2000;48:545-56 pubmed
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Sawada H, Esaki M. A practical technique to postfix nanogold-immunolabeled specimens with osmium and to embed them in Epon for electron microscopy. J Histochem Cytochem. 2000;48:493-8 pubmed
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Robinson J, Takizawa T, Vandre D. Enhanced labeling efficiency using ultrasmall immunogold probes: immunocytochemistry. J Histochem Cytochem. 2000;48:487-92 pubmed
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Hainfeld J, Powell R. New frontiers in gold labeling. J Histochem Cytochem. 2000;48:471-80 pubmed
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Yoshimori T, Yamagata F, Yamamoto A, Mizushima N, Kabeya Y, Nara A, et al. The mouse SKD1, a homologue of yeast Vps4p, is required for normal endosomal trafficking and morphology in mammalian cells. Mol Biol Cell. 2000;11:747-63 pubmed
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Lipschitz D, Michel W. Physiological evidence for the discrimination of L-arginine from structural analogues by the zebrafish olfactory system. J Neurophysiol. 1999;82:3160-7 pubmed
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Bernard V, Levey A, Bloch B. Regulation of the subcellular distribution of m4 muscarinic acetylcholine receptors in striatal neurons in vivo by the cholinergic environment: evidence for regulation of cell surface receptors by endogenous and exogenous stimulation. J Neurosci. 1999;19:10237-49 pubmed
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Kloboucek A, Behrisch A, Faix J, Sackmann E. Adhesion-induced receptor segregation and adhesion plaque formation: A model membrane study. Biophys J. 1999;77:2311-28 pubmed
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Matsuzaki T, Suzuki T, Koyama H, Tanaka S, Takata K. Water channel protein AQP3 is present in epithelia exposed to the environment of possible water loss. J Histochem Cytochem. 1999;47:1275-86 pubmed
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Salas P. Insoluble gamma-tubulin-containing structures are anchored to the apical network of intermediate filaments in polarized CACO-2 epithelial cells. J Cell Biol. 1999;146:645-58 pubmed
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Soussan L, Burakov D, Daniels M, Toister Achituv M, Porat A, Yarden Y, et al. ERG30, a VAP-33-related protein, functions in protein transport mediated by COPI vesicles. J Cell Biol. 1999;146:301-11 pubmed
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Hanson J, Smith Y. Group I metabotropic glutamate receptors at GABAergic synapses in monkeys. J Neurosci. 1999;19:6488-96 pubmed
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Li S, Cheng A, Li H, Li X. Cellular defects and altered gene expression in PC12 cells stably expressing mutant huntingtin. J Neurosci. 1999;19:5159-72 pubmed
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Adams I, Kilmartin J. Localization of core spindle pole body (SPB) components during SPB duplication in Saccharomyces cerevisiae. J Cell Biol. 1999;145:809-23 pubmed
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Suzuki Y, Itakura M, Kashiwagi M, Nakamura N, Matsuki T, Sakuta H, et al. Identification by differential display of a hypertonicity-inducible inward rectifier potassium channel highly expressed in chloride cells. J Biol Chem. 1999;274:11376-82 pubmed
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Gutekunst C, Li S, Yi H, Mulroy J, Kuemmerle S, Jones R, et al. Nuclear and neuropil aggregates in Huntington's disease: relationship to neuropathology. J Neurosci. 1999;19:2522-34 pubmed
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Takizawa T. High-resolution immunocytochemical labeling of replicas with ultrasmall gold. J Histochem Cytochem. 1999;47:569-74 pubmed
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Kessels M, Qualmann B, Thole H, Sierralta W. Subcellular localization of estradiol receptor in MCF7 cells studied with nanogold-labelled antibody fragments. Eur J Histochem. 1998;42:259-70 pubmed
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Gardiol A, Racca C, Triller A. Dendritic and postsynaptic protein synthetic machinery. J Neurosci. 1999;19:168-79 pubmed
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Yoshida M, Wakatsuki Y, Kobayashi Y, Itoh T, Murakami K, Mizoguchi A, et al. Cloning and characterization of a novel membrane-associated antigenic protein of Helicobacter pylori. Infect Immun. 1999;67:286-93 pubmed
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Punnonen E, Fages C, Wartiovaara J, Rauvala H. Ultrastructural localization of beta-actin and amphoterin mRNA in cultured cells: application of tyramide signal amplification and comparison of detection methods. J Histochem Cytochem. 1999;47:99-112 pubmed
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Baschong W, Stierhof Y. Preparation, use, and enlargement of ultrasmall gold particles in immunoelectron microscopy. Microsc Res Tech. 1998;42:66-79 pubmed
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Powell R, Halsey C, Hainfeld J. Combined fluorescent and gold immunoprobes: reagents and methods for correlative light and electron microscopy. Microsc Res Tech. 1998;42:2-12 pubmed
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Somogyi P, Tamas G, Lujan R, Buhl E. Salient features of synaptic organisation in the cerebral cortex. Brain Res Brain Res Rev. 1998;26:113-35 pubmed
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Hainfeld J. Labeling with nanogold and undecagold: techniques and results. Scanning Microsc Suppl. 1996;10:309-22; discussion 322-5 pubmed
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Malecki M. Preparation of plasmid DNA in transfection complexes for fluorescence and electron spectroscopic imaging. Scanning Microsc Suppl. 1996;10:1-16 pubmed
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Du J, Tao Cheng J, Zerfas P, McBain C. The K+ channel, Kv2.1, is apposed to astrocytic processes and is associated with inhibitory postsynaptic membranes in hippocampal and cortical principal neurons and inhibitory interneurons. Neuroscience. 1998;84:37-48 pubmed
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Nusser Z, Sieghart W, Somogyi P. Segregation of different GABAA receptors to synaptic and extrasynaptic membranes of cerebellar granule cells. J Neurosci. 1998;18:1693-703 pubmed
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Lujan R, Roberts J, Shigemoto R, Ohishi H, Somogyi P. Differential plasma membrane distribution of metabotropic glutamate receptors mGluR1 alpha, mGluR2 and mGluR5, relative to neurotransmitter release sites. J Chem Neuroanat. 1997;13:219-41 pubmed
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Thompson W, Beven A, Wells B, Shaw P. Sites of rDNA transcription are widely dispersed through the nucleolus in Pisum sativum and can comprise single genes. Plant J. 1997;12:571-81 pubmed
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Nusser Z, Cull Candy S, Farrant M. Differences in synaptic GABA(A) receptor number underlie variation in GABA mini amplitude. Neuron. 1997;19:697-709 pubmed
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Aoki T, Hagiwara H, Fujimoto T. Peculiar distribution of fodrin in fat-storing cells. Exp Cell Res. 1997;234:313-20 pubmed
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Powell R, Halsey C, Spector D, Kaurin S, McCann J, Hainfeld J. A covalent fluorescent-gold immunoprobe: simultaneous detection of a pre-mRNA splicing factor by light and electron microscopy. J Histochem Cytochem. 1997;45:947-56 pubmed
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Yazama F, Esaki M, Sawada H. Immunocytochemistry of extracellular matrix components in the rat seminiferous tubule: electron microscopic localization with improved methodology. Anat Rec. 1997;248:51-62 pubmed
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Li H, Ohishi H, Kinoshita A, Shigemoto R, Nomura S, Mizuno N. Localization of a metabotropic glutamate receptor, mGluR7, in axon terminals of presumed nociceptive, primary afferent fibers in the superficial layers of the spinal dorsal horn: an electron microscope study in the rat. Neurosci Lett. 1997;223:153-6 pubmed
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Gregori L, Hainfeld J, Simon M, Goldgaber D. Binding of amyloid beta protein to the 20 S proteasome. J Biol Chem. 1997;272:58-62 pubmed
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Robinett C, Straight A, Li G, Willhelm C, Sudlow G, Murray A, et al. In vivo localization of DNA sequences and visualization of large-scale chromatin organization using lac operator/repressor recognition. J Cell Biol. 1996;135:1685-700 pubmed
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Bernard V, Somogyi P, Bolam J. Cellular, subcellular, and subsynaptic distribution of AMPA-type glutamate receptor subunits in the neostriatum of the rat. J Neurosci. 1997;17:819-33 pubmed
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Tanner V, Ploug T, Tao Cheng J. Subcellular localization of SV2 and other secretory vesicle components in PC12 cells by an efficient method of preembedding EM immunocytochemistry for cell cultures. J Histochem Cytochem. 1996;44:1481-8 pubmed
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Nusser Z, Sieghart W, Benke D, Fritschy J, Somogyi P. Differential synaptic localization of two major gamma-aminobutyric acid type A receptor alpha subunits on hippocampal pyramidal cells. Proc Natl Acad Sci U S A. 1996;93:11939-44 pubmed
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Larsson L. Immunogold labelling of neuroendocrine peptides with special reference to antibody specificity and multiple staining techniques. Histochem Cell Biol. 1996;106:93-103 pubmed
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Hermann R, Walther P, Muller M. Immunogold labeling in scanning electron microscopy. Histochem Cell Biol. 1996;106:31-9 pubmed
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Lackie P. Immunogold silver staining for light microscopy. Histochem Cell Biol. 1996;106:9-17 pubmed
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Baude A, Nusser Z, Molnar E, McIlhinney R, Somogyi P. High-resolution immunogold localization of AMPA type glutamate receptor subunits at synaptic and non-synaptic sites in rat hippocampus. Neuroscience. 1995;69:1031-55 pubmed
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Halasy K, Buhl E, Lörinczi Z, Tamas G, Somogyi P. Synaptic target selectivity and input of GABAergic basket and bistratified interneurons in the CA1 area of the rat hippocampus. Hippocampus. 1996;6:306-29 pubmed
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Sawada H, Sugawara I, Kitami A, Hayashi M. Vitronectin in the cytoplasm of Leydig cells in the rat testis. Biol Reprod. 1996;54:29-35 pubmed
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Lujan R, Nusser Z, Roberts J, Shigemoto R, Somogyi P. Perisynaptic location of metabotropic glutamate receptors mGluR1 and mGluR5 on dendrites and dendritic spines in the rat hippocampus. Eur J Neurosci. 1996;8:1488-500 pubmed
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Matsubara A, Laake J, Davanger S, Usami S, Ottersen O. Organization of AMPA receptor subunits at a glutamate synapse: a quantitative immunogold analysis of hair cell synapses in the rat organ of Corti. J Neurosci. 1996;16:4457-67 pubmed
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Shigemoto R, Kulik A, Roberts J, Ohishi H, Nusser Z, Kaneko T, et al. Target-cell-specific concentration of a metabotropic glutamate receptor in the presynaptic active zone. Nature. 1996;381:523-5 pubmed
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Nusser Z, Sieghart W, Stephenson F, Somogyi P. The alpha 6 subunit of the GABAA receptor is concentrated in both inhibitory and excitatory synapses on cerebellar granule cells. J Neurosci. 1996;16:103-14 pubmed
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Griffing L, Villanueva M, Taylor J, Moon S. Confocal epipolarization microscopy of gold probes in plant cells and protoplasts. Methods Cell Biol. 1995;49:109-21 pubmed
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Baude A, Nusser Z, Roberts J, Mulvihill E, McIlhinney R, Somogyi P. The metabotropic glutamate receptor (mGluR1 alpha) is concentrated at perisynaptic membrane of neuronal subpopulations as detected by immunogold reaction. Neuron. 1993;11:771-87 pubmed
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Braig K, Simon M, Furuya F, Hainfeld J, Horwich A. A polypeptide bound by the chaperonin groEL is localized within a central cavity. Proc Natl Acad Sci U S A. 1993;90:3978-82 pubmed
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Mizoguchi A, Yano Y, Hamaguchi H, Yanagida H, Ide C, Zahraoui A, et al. Localization of Rabphilin-3A on the synaptic vesicle. Biochem Biophys Res Commun. 1994;202:1235-43 pubmed
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Krenacs T, Dux L. Silver-enhanced immunogold labeling of calcium-ATPase in sarcoplasmic reticulum of skeletal muscle. J Histochem Cytochem. 1994;42:967-8 pubmed
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Suzuki E, Hirosawa K. Immunolocalization of a Drosophila phosphatidylinositol transfer protein (rdgB) in normal and rdgA mutant photoreceptor cells with special reference to the subrhabdomeric cisternae. J Electron Microsc (Tokyo). 1994;43:183-9 pubmed
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Takizawa T, Robinson J. Use of 1.4-nm immunogold particles for immunocytochemistry on ultra-thin cryosections. J Histochem Cytochem. 1994;42:1615-23 pubmed
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Nusser Z, Mulvihill E, Streit P, Somogyi P. Subsynaptic segregation of metabotropic and ionotropic glutamate receptors as revealed by immunogold localization. Neuroscience. 1994;61:421-7 pubmed
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Gilerovitch H, Bishop G, King J, Burry R. The use of electron microscopic immunocytochemistry with silver-enhanced 1.4-nm gold particles to localize GAD in the cerebellar nuclei. J Histochem Cytochem. 1995;43:337-43 pubmed
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Sun X, Tolbert L, Hildebrand J. Using laser scanning confocal microscopy as a guide for electron microscopic study: a simple method for correlation of light and electron microscopy. J Histochem Cytochem. 1995;43:329-35 pubmed
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Nusser Z, Roberts J, Baude A, Richards J, Somogyi P. Relative densities of synaptic and extrasynaptic GABAA receptors on cerebellar granule cells as determined by a quantitative immunogold method. J Neurosci. 1995;15:2948-60 pubmed
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Sawada H, Esaki M. Use of nanogold followed by silver enhancement and gold toning for preembedding immunolocalization in osmium-fixed, Epon-embedded tissues. J Electron Microsc (Tokyo). 1994;43:361-6 pubmed
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Nixon G, Mignery G, Somlyo A. Immunogold localization of inositol 1,4,5-trisphosphate receptors and characterization of ultrastructural features of the sarcoplasmic reticulum in phasic and tonic smooth muscle. J Muscle Res Cell Motil. 1994;15:682-700 pubmed
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Nusser Z, Roberts J, Baude A, Richards J, Sieghart W, Somogyi P. Immunocytochemical localization of the alpha 1 and beta 2/3 subunits of the GABAA receptor in relation to specific GABAergic synapses in the dentate gyrus. Eur J Neurosci. 1995;7:630-46 pubmed
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Humbel B, Sibon O, Stierhof Y, Schwarz H. Ultra-small gold particles and silver enhancement as a detection system in immunolabeling and in situ hybridization experiments. J Histochem Cytochem. 1995;43:735-7 pubmed
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Hainfeld J, Furuya F. A 1.4-nm gold cluster covalently attached to antibodies improves immunolabeling. J Histochem Cytochem. 1992;40:177-84 pubmed
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Burry R, Vandre D, Hayes D. Silver enhancement of gold antibody probes in pre-embedding electron microscopic immunocytochemistry. J Histochem Cytochem. 1992;40:1849-56 pubmed
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Vandre D, Burry R. Immunoelectron microscopic localization of phosphoproteins associated with the mitotic spindle. J Histochem Cytochem. 1992;40:1837-47 pubmed
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Boisset N, Pochon F, Chwetzoff S, Barray M, Delain E, Lamy J. Electron microscopy of alpha 2-macroglobulin with a thiol ester bound ligand. J Struct Biol. 1992;108:221-6 pubmed
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product information
Product Type :
Secondary Antibody
Product Name :
F(ab')2-Goat anti-Rabbit IgG (H+L) Secondary Antibody, NANOGOLD
Catalog # :
N-24916
Quantity :
1 mL
Price :
US 644.00
Clonality :
Polyclonal
Purity :
purified
Host :
F(ab')2-Goat
Reactivity :
Rabbit
Applications :
Immunomicroscopy: 0.2-10 ug/mL, Western Blot: 0.2-10 ug/mL
Species :
Rabbit
Isotype :
IgG
Storage :
4 C
Description :
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Immunogen :
Gamma Immunoglobin
Format :
Liquid
Applications w/Dilutions :
Immunomicroscopy: 0.2-10 ug/mL, Western Blot: 0.2-10 ug/mL
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com81 Wyman Street
Waltham, MA USA 02451
800-678-5599
headquarters: USA
questions and comments