TNF alpha Monoclonal Antibody (6E10) | Invitrogen MP391 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

TNF alpha Monoclonal Antibody (6E10)

catalog :

MP391

quantity :

500 µg

price :

US 399

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6E10

reactivity :

pigs

application :

western blot, ELISA

citations: 5

Published Application/Species/Sample/Dilution	Reference
ELISA; pigs ; loading ...; fig 1	Wyns H, Croubels S, Demeyere K, Watteyn A, De Backer P, Meyer E. Development of a cytometric bead array screening tool for the simultaneous detection of pro-inflammatory cytokines in porcine plasma. Vet Immunol Immunopathol. 2013;151:28-36 pubmed publisher
	Choi C, Hwang K, Chae C. Classical swine fever virus induces tumor necrosis factor-alpha and lymphocyte apoptosis. Arch Virol. 2004;149:875-89 pubmed
	O Neill C, Liu J, Leibenberg E, Hu S, Deviren V, Tay B, et al. Percutaneous plasma decompression alters cytokine expression in injured porcine intervertebral discs. Spine J. 2004;4:88-98 pubmed
	Trebichavsky I, Splichal I, Zahradnícková M, Splichalova A, Mori Y. Lipopolysaccharide induces inflammatory cytokines in the pig amnion. Vet Immunol Immunopathol. 2002;87:11-8 pubmed
	Su X, Prestwood A, Otto C, Prickett D, McGraw R. Monoclonal antibodies to porcine tumor necrosis factor alpha: development of an enzyme-linked immunosorbent assay. J Immunoassay. 1992;13:253-72 pubmed

product information

Product Type :

Antibody

Product Name :

TNF alpha Monoclonal Antibody (6E10)

Catalog # :

MP391

Quantity :

500 µg

Price :

US 399

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Porcine

Applications :

ELISA: 3 µg/mL (coating), Western Blot: 0.02 µg/mL

Species :

Porcine

Clone :

6E10

Isotype :

IgG1

Storage :

-20°C

Description :

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Immunogen :

Recombinant porcine TNF alpha (Baculovirus-derived)

Format :

Liquid

Applications w/Dilutions :

ELISA: 3 µg/mL (coating), Western Blot: 0.02 µg/mL

Aliases :

APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor