TNF alpha Monoclonal Antibody (4F4) | Invitrogen MP390 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

TNF alpha Monoclonal Antibody (4F4)

catalog :

MP390

quantity :

500µg

price :

US 345

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

4F4

reactivity :

rat, pigs

application :

western blot, ELISA, immunohistochemistry, flow cytometry

more info or order :

Invitrogen product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
ELISA; pigs ; loading ...; fig 1	Wyns H, Croubels S, Demeyere K, Watteyn A, De Backer P, Meyer E. Development of a cytometric bead array screening tool for the simultaneous detection of pro-inflammatory cytokines in porcine plasma. Vet Immunol Immunopathol. 2013;151:28-36 pubmed publisher
immunohistochemistry; pigs	Nadworny P, Landry B, Wang J, Tredget E, Burrell R. Does nanocrystalline silver have a transferable effect?. Wound Repair Regen. 2010;18:254-65 pubmed publisher
immunohistochemistry; pigs	Nadworny P, Wang J, Tredget E, Burrell R. Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis. J Inflamm (Lond). 2010;7:13 pubmed publisher
immunohistochemistry; rat; 1:150	Cayci C, Wahlquist T, Seckin S, Ozcan V, Tekinay A, Martens T, et al. Naringenin inhibits neointimal hyperplasia following arterial reconstruction with interpositional vein graft. Ann Plast Surg. 2010;64:105-13 pubmed publisher
flow cytometry; pigs	Charerntantanakul W, Platt R, Roth J. Effects of porcine reproductive and respiratory syndrome virus-infected antigen-presenting cells on T cell activation and antiviral cytokine production. Viral Immunol. 2006;19:646-61 pubmed
immunohistochemistry; pigs ; 1:100	Murata Y, Olmarker K, Larsson K, Takahashi K, Rydevik B. Production of tumor necrosis factor-alpha from porcine nucleus pulposus cells at various time points in cell culture under conditions of nutritional deficiency. Cytokine. 2006;34:206-11 pubmed
	Salguero F, Mekonnen T, Ruiz Villamor E, Sánchez Cordón P, Gomez Villamandos J. Detection of monokines in paraffin-embedded tissues of pigs using polyclonal antibodies. Vet Res. 2001;32:601-9 pubmed
	Knoetig S, McCullough K, Summerfield A. Lipopolysaccharide-induced impairment of classical swine fever virus infection in monocytic cells is sensitive to 2-aminopurine. Antiviral Res. 2002;53:75-81 pubmed
	Gomez del Moral M, Ortuño E, Fernandez Zapatero P, Alonso F, Alonso C, Ezquerra A, et al. African swine fever virus infection induces tumor necrosis factor alpha production: implications in pathogenesis. J Virol. 1999;73:2173-80 pubmed
	Trebichavsky I, Dlabac V, Rehakova Z, Zahradnícková M, Splichal I. Cellular changes and cytokine expression in the ilea of gnotobiotic piglets resulting from peroral Salmonella typhimurium challenge. Infect Immun. 1997;65:5244-9 pubmed
	Su X, Prestwood A, Otto C, Prickett D, McGraw R. Monoclonal antibodies to porcine tumor necrosis factor alpha: development of an enzyme-linked immunosorbent assay. J Immunoassay. 1992;13:253-72 pubmed

product information

Product Type :

Antibody

Product Name :

TNF alpha Monoclonal Antibody (4F4)

Catalog # :

MP390

Quantity :

500µg

Price :

US 345

Clonality :

Monoclonal

Purity :

protein A

Host :

Mouse

Reactivity :

Porcine

Applications :

ELISA: 0.1-0.25 µg/mL (coating), Western Blot: 0.02 µg/mL

Species :

Porcine

Clone :

4F4

Isotype :

IgG1

Storage :

-20°C

Description :

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Immunogen :

Recombinant porcine TNF alpha (Baculovirus-derived)

Format :

Liquid

Applications w/Dilutions :

ELISA: 0.1-0.25 µg/mL (coating), Western Blot: 0.02 µg/mL

Aliases :

APC1 protein; BOS_21587; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; SBAB-548A10.2; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; Tnfa; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; Tnfsf2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor ligand 1F; Tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor-necrosis factor; tumour necrosis factor

more info or order :

Invitrogen product webpage