IFN gamma Monoclonal Antibody (R4-6A2) | Invitrogen MM701 product information

product summary

company name :

Invitrogen

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product type :

antibody

product name :

IFN gamma Monoclonal Antibody (R4-6A2)

catalog :

MM701

quantity :

500 µg

price :

US 391

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

R4-6A2

reactivity :

mouse

application :

western blot, ELISA, immunohistochemistry, neutralization, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 72

Published Application/Species/Sample/Dilution	Reference
ELISA; mouse; loading ...; fig 3a	Li L, Labuda J, Imai D, Griffey S, McSorley S. CCR7 Deficiency Allows Accelerated Clearance of Chlamydia from the Female Reproductive Tract. J Immunol. 2017;199:2547-2554 pubmed publisher
blocking or activating experiments; mouse; loading ...; fig 4a	Okuyama H, Tominaga A, Fukuoka S, Taguchi T, Kusumoto Y, Ono S. Spirulina lipopolysaccharides inhibit tumor growth in a Toll-like receptor 4-dependent manner by altering the cytokine milieu from interleukin-17/interleukin-23 to interferon-?. Oncol Rep. 2017;37:684-694 pubmed publisher
ELISA; mouse; loading ...; fig s7a	Griffiths K, Ahmed M, Das S, Gopal R, Horne W, Connell T, et al. Targeting dendritic cells to accelerate T-cell activation overcomes a bottleneck in tuberculosis vaccine efficacy. Nat Commun. 2016;7:13894 pubmed publisher
western blot; mouse; fig 3	Ahmad F, Chung Y, Tang Y, Hockman S, Liu S, Khan Y, et al. Phosphodiesterase 3B (PDE3B) regulates NLRP3 inflammasome in adipose tissue. Sci Rep. 2016;6:28056 pubmed publisher
flow cytometry; mouse; 1:100; fig s6b	Bulla R, Tripodo C, Rami D, Ling G, Agostinis C, Guarnotta C, et al. C1q acts in the tumour microenvironment as a cancer-promoting factor independently of complement activation. Nat Commun. 2016;7:10346 pubmed publisher
blocking or activating experiments; mouse	Sekiya T, Yoshimura A. In Vitro Th Differentiation Protocol. Methods Mol Biol. 2016;1344:183-91 pubmed publisher
ELISA; mouse; fig 3	Uchiyama M, Jin X, Yin E, Shimokawa T, Niimi M. Treadmill exercise induces murine cardiac allograft survival and generates regulatory T cell. Transpl Int. 2015;28:352-62 pubmed publisher
ELISA; mouse	Newton M, Askeland E, Andresen E, Chehval V, Wang X, Askeland R, et al. Anti-interleukin-10R1 monoclonal antibody in combination with bacillus Calmette--Guérin is protective against bladder cancer metastasis in a murine orthotopic tumour model and demonstrates systemic specific anti-tumour immunity. Clin Exp Immunol. 2014;177:261-8 pubmed publisher
ELISA; mouse; fig 3	Jin X, Uchiyama M, Zhang Q, Hirai T, Niimi M. Inchingorei-san (TJ-117) and Artemisiae Capillaris Herba Induced Prolonged Survival of Fully Mismatched Cardiac Allografts and Generated Regulatory Cells in Mice. Evid Based Complement Alternat Med. 2012;2012:689810 pubmed publisher
ELISA; mouse; fig 3	Uchiyama M, Jin X, Zhang Q, Hirai T, Amano A, Bashuda H, et al. Auditory stimulation of opera music induced prolongation of murine cardiac allograft survival and maintained generation of regulatory CD4+CD25+ cells. J Cardiothorac Surg. 2012;7:26 pubmed publisher
ELISA; mouse	Liu W, Chen X, Evanoff D, Luo Y. Urothelial antigen-specific CD4+ T cells function as direct effector cells and induce bladder autoimmune inflammation independent of CD8+ T cells. Mucosal Immunol. 2011;4:428-37 pubmed publisher
ELISA; mouse	Del Pozzo G, Mascolo D, Sartorius R, Citro A, Barba P, D Apice L, et al. Triggering DTH and CTL activity by fd filamentous bacteriophages: role of CD4+ T cells in memory responses. J Biomed Biotechnol. 2010;2010:894971 pubmed publisher
ELISA; mouse	Hava D, van der Wel N, Cohen N, Dascher C, Houben D, Leon L, et al. Evasion of peptide, but not lipid antigen presentation, through pathogen-induced dendritic cell maturation. Proc Natl Acad Sci U S A. 2008;105:11281-6 pubmed publisher
ELISA; mouse	Akbari O, Stock P, Meyer E, Freeman G, Sharpe A, Umetsu D, et al. ICOS/ICOSL interaction is required for CD4+ invariant NKT cell function and homeostatic survival. J Immunol. 2008;180:5448-56 pubmed
ELISA; mouse	Croce M, Meazza R, Orengo A, Fabbi M, Borghi M, Ribatti D, et al. Immunotherapy of neuroblastoma by an Interleukin-21-secreting cell vaccine involves survivin as antigen. Cancer Immunol Immunother. 2008;57:1625-34 pubmed publisher
ELISA; mouse; 10 ug/ml	Tian C, Ansari M, Paez Cortez J, Bagley J, Godwin J, Donnarumma M, et al. Induction of robust diabetes resistance and prevention of recurrent type 1 diabetes following islet transplantation by gene therapy. J Immunol. 2007;179:6762-9 pubmed
ELISA; mouse	Hara H, Kawamura I, Nomura T, Tominaga T, Tsuchiya K, Mitsuyama M. Cytolysin-dependent escape of the bacterium from the phagosome is required but not sufficient for induction of the Th1 immune response against Listeria monocytogenes infection: distinct role of Listeriolysin O determined by cytolysin gene replacement. Infect Immun. 2007;75:3791-801 pubmed
ELISA; mouse; 2 ug/ml	Tsunetsugu Yokota Y, Ishige M, Murakami M. Oral attenuated Salmonella enterica serovar Typhimurium vaccine expressing codon-optimized HIV type 1 Gag enhanced intestinal immunity in mice. AIDS Res Hum Retroviruses. 2007;23:278-86 pubmed
ELISA; mouse	Roach D, Briscoe H, Saunders B, Britton W. Independent protective effects for tumor necrosis factor and lymphotoxin alpha in the host response to Listeria monocytogenes infection. Infect Immun. 2005;73:4787-92 pubmed
ELISA; mouse	Puddu P, Carollo M, Pietraforte I, Spadaro F, Tombesi M, Ramoni C, et al. IL-2 induces expression and secretion of IFN-gamma in murine peritoneal macrophages. J Leukoc Biol. 2005;78:686-95 pubmed
ELISA; mouse; fig 4	Aramaki O, Shirasugi N, Takayama T, Shimazu M, Kitajima M, Ikeda Y, et al. Programmed death-1-programmed death-L1 interaction is essential for induction of regulatory cells by intratracheal delivery of alloantigen. Transplantation. 2004;77:6-12 pubmed
ELISA; mouse	Roach D, Bean A, Demangel C, France M, Briscoe H, Britton W. TNF regulates chemokine induction essential for cell recruitment, granuloma formation, and clearance of mycobacterial infection. J Immunol. 2002;168:4620-7 pubmed
	Martin M, Sompallae R, Winborn C, Harty J, Badovinac V. Diverse CD8 T Cell Responses to Viral Infection Revealed by the Collaborative Cross. Cell Rep. 2020;31:107508 pubmed publisher
	Graham K, Werner B, Moyer K, Patton A, Krois C, Yoo H, et al. DGAT1 inhibits retinol-dependent regulatory T cell formation and mediates autoimmune encephalomyelitis. Proc Natl Acad Sci U S A. 2019;116:3126-3135 pubmed publisher
	Yanagisawa N, Ueshiba H, Abe Y, Kato H, Higuchi T, Yagi J. Outer Membrane Protein of Gut Commensal Microorganism Induces Autoantibody Production and Extra-Intestinal Gland Inflammation in Mice. Int J Mol Sci. 2018;19: pubmed publisher
	Nalawade S, Ji N, Raphael I, Pratt A, Kraig E, Forsthuber T. Aire is not essential for regulating neuroinflammatory disease in mice transgenic for human autoimmune-diseases associated MHC class II genes HLA-DR2b and HLA-DR4. Cell Immunol. 2018;331:38-48 pubmed publisher
	Cerqueira Rodrigues B, Mendes A, Correia Neves M, Nobrega C. Ag85-focused T-cell immune response controls Mycobacterium avium chronic infection. PLoS ONE. 2018;13:e0193596 pubmed publisher
	Carpier J, Zucchetti A, Bataille L, Dogniaux S, Shafaq Zadah M, Bardin S, et al. Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation. J Exp Med. 2018;215:1245-1265 pubmed publisher
	Zhao M, Tan Y, Peng Q, Huang C, Guo Y, Liang G, et al. IL-6/STAT3 pathway induced deficiency of RFX1 contributes to Th17-dependent autoimmune diseases via epigenetic regulation. Nat Commun. 2018;9:583 pubmed publisher
	Koyanagi N, Imai T, Shindo K, Sato A, Fujii W, Ichinohe T, et al. Herpes simplex virus-1 evasion of CD8+ T cell accumulation contributes to viral encephalitis. J Clin Invest. 2017;127:3784-3795 pubmed publisher
	Rowe A, Yun H, Hendricks R. Exposure Stress Induces Reversible Corneal Graft Opacity in Recipients With Herpes Simplex Virus-1 Infections. Invest Ophthalmol Vis Sci. 2017;58:35-41 pubmed publisher
	Ho S, Zhang W, Tan N, Khatoo M, Suter M, Tripathi S, et al. The DNA Structure-Specific Endonuclease MUS81 Mediates DNA Sensor STING-Dependent Host Rejection of Prostate Cancer Cells. Immunity. 2016;44:1177-89 pubmed publisher
	Valcourt U, Carthy J, Okita Y, Alcaraz L, Kato M, Thuault S, et al. Analysis of Epithelial-Mesenchymal Transition Induced by Transforming Growth Factor Î². Methods Mol Biol. 2016;1344:147-81 pubmed publisher
	Castillo E, Dekonenko A, Arko Mensah J, Mandell M, Dupont N, Jiang S, et al. Autophagy protects against active tuberculosis by suppressing bacterial burden and inflammation. Proc Natl Acad Sci U S A. 2012;109:E3168-76 pubmed publisher
	Guzik T, Hoch N, Brown K, McCann L, Rahman A, Dikalov S, et al. Role of the T cell in the genesis of angiotensin II induced hypertension and vascular dysfunction. J Exp Med. 2007;204:2449-60 pubmed
	Michel M, Keller A, Paget C, Fujio M, Trottein F, Savage P, et al. Identification of an IL-17-producing NK1.1(neg) iNKT cell population involved in airway neutrophilia. J Exp Med. 2007;204:995-1001 pubmed
	Liang S, Tan X, Luxenberg D, Karim R, Dunussi Joannopoulos K, Collins M, et al. Interleukin (IL)-22 and IL-17 are coexpressed by Th17 cells and cooperatively enhance expression of antimicrobial peptides. J Exp Med. 2006;203:2271-9 pubmed
	Boyman O, Cho J, Tan J, Surh C, Sprent J. A major histocompatibility complex class I-dependent subset of memory phenotype CD8+ cells. J Exp Med. 2006;203:1817-25 pubmed
	Saunders B, Briscoe H, Britton W. T cell-derived tumour necrosis factor is essential, but not sufficient, for protection against Mycobacterium tuberculosis infection. Clin Exp Immunol. 2004;137:279-87 pubmed
	Luo Y, Yamada H, Chen X, Ryan A, Evanoff D, Triccas J, et al. Recombinant Mycobacterium bovis bacillus Calmette-Guérin (BCG) expressing mouse IL-18 augments Th1 immunity and macrophage cytotoxicity. Clin Exp Immunol. 2004;137:24-34 pubmed
	Chaudhri G, Panchanathan V, Buller R, van den Eertwegh A, Claassen E, Zhou J, et al. Polarized type 1 cytokine response and cell-mediated immunity determine genetic resistance to mousepox. Proc Natl Acad Sci U S A. 2004;101:9057-62 pubmed
	Antúnez M, Cardoni R. Trypanosoma cruzi: the expansion of NK, T, and NKT cells in the experimental infection. Exp Parasitol. 2004;106:85-94 pubmed
	Tokoyoda K, Tsujikawa K, Matsushita H, Ono Y, Hayashi T, Harada Y, et al. Up-regulation of IL-4 production by the activated cAMP/cAMP-dependent protein kinase (protein kinase A) pathway in CD3/CD28-stimulated naive T cells. Int Immunol. 2004;16:643-53 pubmed
	Matsuura M, Saito S, Hirai Y, Okamura H. A pathway through interferon-gamma is the main pathway for induction of nitric oxide upon stimulation with bacterial lipopolysaccharide in mouse peritoneal cells. Eur J Biochem. 2003;270:4016-25 pubmed
	Yoshizawa I, Mizuochi T, Ogata A, Murakami M, Yagita H, Takahashi Y, et al. Studies on the generation and maintenance of mucosal cytotoxic T lymphocytes against human immunodeficiency virus type 1 Gag in mice. AIDS Res Hum Retroviruses. 2003;19:469-79 pubmed
	Hufnagl K, Wagner B, Winkler B, Baier K, Hochreiter R, Thalhamer J, et al. Induction of mucosal tolerance with recombinant Hev b 1 and recombinant Hev b 3 for prevention of latex allergy in BALB/c mice. Clin Exp Immunol. 2003;133:170-6 pubmed
	Lang T, Courret N, Colle J, Milon G, Antoine J. The levels and patterns of cytokines produced by CD4 T lymphocytes of BALB/c mice infected with Leishmania major by inoculation into the ear dermis depend on the infectiousness and size of the inoculum. Infect Immun. 2003;71:2674-83 pubmed
	Hill H, Conway T, Sabel M, Jong Y, Mathiowitz E, Bankert R, et al. Cancer immunotherapy with interleukin 12 and granulocyte-macrophage colony-stimulating factor-encapsulated microspheres: coinduction of innate and adaptive antitumor immunity and cure of disseminated disease. Cancer Res. 2002;62:7254-63 pubmed
	Hess S, Egilmez N, Bailey N, Anderson T, Mathiowitz E, Bernstein S, et al. Human CD4+ T cells present within the microenvironment of human lung tumors are mobilized by the local and sustained release of IL-12 to kill tumors in situ by indirect effects of IFN-gamma. J Immunol. 2003;170:400-12 pubmed
	Kohda C, Kawamura I, Baba H, Nomura T, Ito Y, Kimoto T, et al. Dissociated linkage of cytokine-inducing activity and cytotoxicity to different domains of listeriolysin O from Listeria monocytogenes. Infect Immun. 2002;70:1334-41 pubmed
	Smith R, Harris S, Phipps R, Iglewski B. The Pseudomonas aeruginosa quorum-sensing molecule N-(3-oxododecanoyl)homoserine lactone contributes to virulence and induces inflammation in vivo. J Bacteriol. 2002;184:1132-9 pubmed
	Chitnis T, Najafian N, Benou C, Salama A, Grusby M, Sayegh M, et al. Effect of targeted disruption of STAT4 and STAT6 on the induction of experimental autoimmune encephalomyelitis. J Clin Invest. 2001;108:739-47 pubmed
	Jyonouchi H, Sun S, Winship T, Kuchan M. Dietary ribonucleotides modulate type 1 and type 2 T-helper cell responses against ovalbumin in young BALB/cJ mice. J Nutr. 2001;131:1165-70 pubmed
	Chitnis T, Najafian N, Abdallah K, Dong V, Yagita H, Sayegh M, et al. CD28-independent induction of experimental autoimmune encephalomyelitis. J Clin Invest. 2001;107:575-83 pubmed
	Martin E, Roche P, Triccas J, Britton W. DNA encoding a single mycobacterial antigen protects against leprosy infection. Vaccine. 2001;19:1391-6 pubmed
	Yamamoto N, Suzuki S, Suzuki Y, Shirai A, Nakazawa M, Suzuki M, et al. Immune response induced by airway sensitization after influenza A virus infection depends on timing of antigen exposure in mice. J Virol. 2001;75:499-505 pubmed
	Rolph M, Kaufmann S. Partially TAP-independent protection against Listeria monocytogenes by H2-M3-restricted CD8+ T cells. J Immunol. 2000;165:4575-80 pubmed
	Martin E, Kamath A, Triccas J, Britton W. Protection against virulent Mycobacterium avium infection following DNA vaccination with the 35-kilodalton antigen is accompanied by induction of gamma interferon-secreting CD4(+) T cells. Infect Immun. 2000;68:3090-6 pubmed
	Kamath A, Hanke T, Briscoe H, Britton W. Co-immunization with DNA vaccines expressing granulocyte-macrophage colony-stimulating factor and mycobacterial secreted proteins enhances T-cell immunity, but not protective efficacy against Mycobacterium tuberculosis. Immunology. 1999;96:511-6 pubmed
	Bean A, Roach D, Briscoe H, France M, Korner H, Sedgwick J, et al. Structural deficiencies in granuloma formation in TNF gene-targeted mice underlie the heightened susceptibility to aerosol Mycobacterium tuberculosis infection, which is not compensated for by lymphotoxin. J Immunol. 1999;162:3504-11 pubmed
	Kamath A, Feng C, Macdonald M, Briscoe H, Britton W. Differential protective efficacy of DNA vaccines expressing secreted proteins of Mycobacterium tuberculosis. Infect Immun. 1999;67:1702-7 pubmed
	Assenmacher M, Lohning M, Scheffold A, Richter A, Miltenyi S, Schmitz J, et al. Commitment of individual Th1-like lymphocytes to expression of IFN-gamma versus IL-4 and IL-10: selective induction of IL-10 by sequential stimulation of naive Th cells with IL-12 and IL-4. J Immunol. 1998;161:2825-32 pubmed
	Bordmann G, Rudin W, Favre N. Immunization of mice with phosphatidylcholine drastically reduces the parasitaemia of subsequent Plasmodium chabaudi chabaudi blood-stage infections. Immunology. 1998;94:35-40 pubmed
	Targoni O, Lehmann P. Endogenous myelin basic protein inactivates the high avidity T cell repertoire. J Exp Med. 1998;187:2055-63 pubmed
	Miner K, Croft M. Generation, persistence, and modulation of Th0 effector cells: role of autocrine IL-4 and IFN-gamma. J Immunol. 1998;160:5280-7 pubmed
	Flamand V, Donckier V, Demoor F, Le Moine A, Matthys P, Vanderhaeghen M, et al. CD40 ligation prevents neonatal induction of transplantation tolerance. J Immunol. 1998;160:4666-9 pubmed
	Tsung K, Meko J, Tsung Y, Peplinski G, Norton J. Immune response against large tumors eradicated by treatment with cyclophosphamide and IL-12. J Immunol. 1998;160:1369-77 pubmed
	Chu R, Targoni O, Krieg A, Lehmann P, Harding C. CpG oligodeoxynucleotides act as adjuvants that switch on T helper 1 (Th1) immunity. J Exp Med. 1997;186:1623-31 pubmed
	Williams D, Grubbs B, Pack E, Kelly K, Rank R. Humoral and cellular immunity in secondary infection due to murine Chlamydia trachomatis. Infect Immun. 1997;65:2876-82 pubmed
	Pearlman E, Kazura J, Hazlett F, Boom W. Modulation of murine cytokine responses to mycobacterial antigens by helminth-induced T helper 2 cell responses. J Immunol. 1993;151:4857-64 pubmed
	Morris S, Madden K, Adamovicz J, Gause W, Hubbard B, Gately M, et al. Effects of IL-12 on in vivo cytokine gene expression and Ig isotype selection. J Immunol. 1994;152:1047-56 pubmed
	Taguchi T, McGhee J, Coffman R, Beagley K, Eldridge J, Takatsu K, et al. Analysis of Th1 and Th2 cells in murine gut-associated tissues. Frequencies of CD4+ and CD8+ T cells that secrete IFN-gamma and IL-5. J Immunol. 1990;145:68-77 pubmed

product information

Product Type :

Antibody

Product Name :

IFN gamma Monoclonal Antibody (R4-6A2)

Catalog # :

MM701

Quantity :

500 µg

Price :

US 391

Clonality :

Monoclonal

Purity :

protein A

Host :

Rat

Reactivity :

Mouse

Applications :

ELISA: Assay-dependent, Immunohistochemistry (Frozen): Assay-dependent, Neutralization: Assay-dependent, Western Blot: Assay-dependent

Species :

Mouse

Clone :

R4-6A2

Isotype :

IgG1

Storage :

-20°C

Description :

IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.

Immunogen :

Recombinant mouse IFN gamma

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Immunohistochemistry (Frozen): Assay-dependent, Neutralization: Assay-dependent, Western Blot: Assay-dependent

Aliases :

BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN ?; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFN?; Immune interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon ?; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferon?; M-IFN-g; R-IFN-g

more info or order :

Invitrogen product webpage