TNF alpha Monoclonal Antibody (XT22), Biotin | Invitrogen MM350DB product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

TNF alpha Monoclonal Antibody (XT22), Biotin

catalog :

MM350DB

quantity :

250 µg

price :

US 430.00

clonality :

monoclonal

host :

rat

conjugate :

biotin

clone name :

XT22

reactivity :

mouse

application :

western blot, ELISA, neutralization, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
blocking or activating experiments; mouse	Zhang Z, Li Z, Yan S, Wang X, Abecassis M. TNF-alpha signaling is not required for in vivo transcriptional reactivation of latent murine cytomegalovirus. Transplantation. 2009;88:640-5 pubmed publisher
ELISA; mouse	Albert E, Marshall J. Aging in the absence of TLR2 is associated with reduced IFN-gamma responses in the large intestine and increased severity of induced colitis. J Leukoc Biol. 2008;83:833-42 pubmed publisher
blocking or activating experiments; mouse	Karlsson A, Jägervall K, Utkovic H, Karlsson L, Rehnström E, Fredin M, et al. Intra-colonic administration of the TLR7 agonist R-848 induces an acute local and systemic inflammation in mice. Biochem Biophys Res Commun. 2008;367:242-8 pubmed
ELISA; mouse; 0.25 ug/ml	Sherry C, O Connor J, Kramer J, Freund G. Augmented lipopolysaccharide-induced TNF-alpha production by peritoneal macrophages in type 2 diabetic mice is dependent on elevated glucose and requires p38 MAPK. J Immunol. 2007;178:663-70 pubmed
ELISA; mouse	Mane V, Toietta G, McCormack W, Conde I, Clarke C, Palmer D, et al. Modulation of TNFalpha, a determinant of acute toxicity associated with systemic delivery of first-generation and helper-dependent adenoviral vectors. Gene Ther. 2006;13:1272-80 pubmed
ELISA; mouse	Johnson D, O Connor J, Dantzer R, Freund G. Inhibition of vagally mediated immune-to-brain signaling by vanadyl sulfate speeds recovery from sickness. Proc Natl Acad Sci U S A. 2005;102:15184-9 pubmed
	Myers K, Murthy S, Flanigan A, Witchell D, Butler M, Murray S, et al. Antisense oligonucleotide blockade of tumor necrosis factor-alpha in two murine models of colitis. J Pharmacol Exp Ther. 2003;304:411-24 pubmed
	Zen Y, Harada K, Sasaki M, Tsuneyama K, Katayanagi K, Yamamoto Y, et al. Lipopolysaccharide induces overexpression of MUC2 and MUC5AC in cultured biliary epithelial cells: possible key phenomenon of hepatolithiasis. Am J Pathol. 2002;161:1475-84 pubmed
	Ato M, Iwabuchi K, Shimada S, Mukaida N, Onoe K. Augmented expression of tumour necrosis factor-alpha induced by lipopolysaccharide in spleen of human monocyte chemoattractant protein-1 transgenic mouse enhances the lipopolysaccharide sensitivity of the marginal zone macrophages. Immunology. 2002;106:554-63 pubmed
	Nicoletti F, Di Marco R, Zaccone P, Salvaggio A, Magro G, Bendtzen K, et al. Murine concanavalin A-induced hepatitis is prevented by interleukin 12 (IL-12) antibody and exacerbated by exogenous IL-12 through an interferon-gamma-dependent mechanism. Hepatology. 2000;32:728-33 pubmed
	Lundberg A, Granger N, Russell J, Callicutt S, Gaber L, Kotb M, et al. Temporal correlation of tumor necrosis factor-alpha release, upregulation of pulmonary ICAM-1 and VCAM-1, neutrophil sequestration, and lung injury in diet-induced pancreatitis. J Gastrointest Surg. 2000;4:248-57 pubmed

product information

Product Type :

Antibody

Product Name :

TNF alpha Monoclonal Antibody (XT22), Biotin

Catalog # :

MM350DB

Quantity :

250 µg

Price :

US 430.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Rat

Reactivity :

Mouse

Applications :

ELISA: Assay-dependent, Neutralization: Assay-dependent, Western Blot: Assay-dependent

Species :

Mouse

Clone :

XT22

Isotype :

IgG1

Storage :

-20°C

Description :

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Immunogen :

Recombinant mouse TNF alpha

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Neutralization: Assay-dependent, Western Blot: Assay-dependent

Aliases :

APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor

more info or order :

Invitrogen product webpage