TNF alpha Monoclonal Antibody (XT22) | Invitrogen MM350D product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

TNF alpha Monoclonal Antibody (XT22)

catalog :

MM350D

quantity :

500 µg

price :

US 430.00

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

XT22

reactivity :

mouse

application :

western blot, ELISA, neutralization, flow cytometry, blocking or activating experiments

more info or order :

Invitrogen product webpage

citations: 27

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; loading ...; fig 4f	FÃ¶rster M, Boora R, Petrov J, Fodil N, Albanese I, Kim J, et al. A role for the histone H2A deubiquitinase MYSM1 in maintenance of CD8+ T cells. Immunology. 2017;151:110-121 pubmed publisher
flow cytometry; mouse; fig 3	Yang L, Carrillo M, Wu Y, DiAngelo S, Silveyra P, Umstead T, et al. SP-R210 (Myo18A) Isoforms as Intrinsic Modulators of Macrophage Priming and Activation. PLoS ONE. 2015;10:e0126576 pubmed publisher
flow cytometry; mouse	Smith N, Wissink E, Wang J, Pinello J, Davenport M, Grimson A, et al. Rapid proliferation and differentiation impairs the development of memory CD8+ T cells in early life. J Immunol. 2014;193:177-84 pubmed publisher
blocking or activating experiments; mouse	Sobo Vujanovic A, Munich S, Vujanovic N. Dendritic-cell exosomes cross-present Toll-like receptor-ligands and activate bystander dendritic cells. Cell Immunol. 2014;289:119-27 pubmed publisher
blocking or activating experiments; mouse	Zhang Z, Li Z, Yan S, Wang X, Abecassis M. TNF-alpha signaling is not required for in vivo transcriptional reactivation of latent murine cytomegalovirus. Transplantation. 2009;88:640-5 pubmed publisher
flow cytometry; mouse; fig 2	Ahonen C, Wasiuk A, Fuse S, Turk M, Ernstoff M, Suriawinata A, et al. Enhanced efficacy and reduced toxicity of multifactorial adjuvants compared with unitary adjuvants as cancer vaccines. Blood. 2008;111:3116-25 pubmed publisher
blocking or activating experiments; mouse	Karlsson A, Jägervall K, Utkovic H, Karlsson L, Rehnström E, Fredin M, et al. Intra-colonic administration of the TLR7 agonist R-848 induces an acute local and systemic inflammation in mice. Biochem Biophys Res Commun. 2008;367:242-8 pubmed
ELISA; mouse	Mane V, Toietta G, McCormack W, Conde I, Clarke C, Palmer D, et al. Modulation of TNFalpha, a determinant of acute toxicity associated with systemic delivery of first-generation and helper-dependent adenoviral vectors. Gene Ther. 2006;13:1272-80 pubmed
ELISA; mouse	Guiducci C, Vicari A, Sangaletti S, Trinchieri G, Colombo M. Redirecting in vivo elicited tumor infiltrating macrophages and dendritic cells towards tumor rejection. Cancer Res. 2005;65:3437-46 pubmed
	Song Y, Li Y, Xiao Y, Hu W, Wang X, Wang P, et al. Neutralization of interleukin-17A alleviates burn-induced intestinal barrier disruption via reducing pro-inflammatory cytokines in a mouse model. Burns Trauma. 2019;7:37 pubmed publisher
	Wheeler D, Sariol A, Meyerholz D, Perlman S. Microglia are required for protection against lethal coronavirus encephalitis in mice. J Clin Invest. 2018;128:931-943 pubmed publisher
	Wasiuk A, Testa J, Weidlick J, Sisson C, Vitale L, Widger J, et al. CD27-Mediated Regulatory T Cell Depletion and Effector T Cell Costimulation Both Contribute to Antitumor Efficacy. J Immunol. 2017;199:4110-4123 pubmed publisher
	Taylor N, Vick S, Iglesia M, Brickey W, Midkiff B, McKinnon K, et al. Treg depletion potentiates checkpoint inhibition in claudin-low breast cancer. J Clin Invest. 2017;127:3472-3483 pubmed publisher
	Vicetti Miguel R, Quispe Calla N, Dixon D, Foster R, Gambotto A, Pavelko S, et al. IL-4-secreting eosinophils promote endometrial stromal cell proliferation and prevent Chlamydia-induced upper genital tract damage. Proc Natl Acad Sci U S A. 2017;114:E6892-E6901 pubmed publisher
	Miosge L, Sontani Y, Chuah A, Horikawa K, Russell T, Mei Y, et al. Systems-guided forward genetic screen reveals a critical role of the replication stress response protein ETAA1 in T cell clonal expansion. Proc Natl Acad Sci U S A. 2017;114:E5216-E5225 pubmed publisher
	Miao T, Symonds A, Singh R, Symonds J, Ogbe A, Omodho B, et al. Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation. J Exp Med. 2017;214:1787-1808 pubmed publisher
	Ansa Addo E, Zhang Y, Yang Y, Hussey G, Howley B, Salem M, et al. Membrane-organizing protein moesin controls Treg differentiation and antitumor immunity via TGF-β signaling. J Clin Invest. 2017;127:1321-1337 pubmed publisher
	Hwang S, Cobb D, Bhadra R, Youngblood B, Khan I. Blimp-1-mediated CD4 T cell exhaustion causes CD8 T cell dysfunction during chronic toxoplasmosis. J Exp Med. 2016;213:1799-818 pubmed publisher
	Gabbita S, Johnson M, Kobritz N, Eslami P, Poteshkina A, Varadarajan S, et al. Oral TNFα Modulation Alters Neutrophil Infiltration, Improves Cognition and Diminishes Tau and Amyloid Pathology in the 3xTgAD Mouse Model. PLoS ONE. 2015;10:e0137305 pubmed publisher
	Myers K, Murthy S, Flanigan A, Witchell D, Butler M, Murray S, et al. Antisense oligonucleotide blockade of tumor necrosis factor-alpha in two murine models of colitis. J Pharmacol Exp Ther. 2003;304:411-24 pubmed
	Zen Y, Harada K, Sasaki M, Tsuneyama K, Katayanagi K, Yamamoto Y, et al. Lipopolysaccharide induces overexpression of MUC2 and MUC5AC in cultured biliary epithelial cells: possible key phenomenon of hepatolithiasis. Am J Pathol. 2002;161:1475-84 pubmed
	Ato M, Iwabuchi K, Shimada S, Mukaida N, Onoe K. Augmented expression of tumour necrosis factor-alpha induced by lipopolysaccharide in spleen of human monocyte chemoattractant protein-1 transgenic mouse enhances the lipopolysaccharide sensitivity of the marginal zone macrophages. Immunology. 2002;106:554-63 pubmed
	Nicoletti F, Di Marco R, Zaccone P, Salvaggio A, Magro G, Bendtzen K, et al. Murine concanavalin A-induced hepatitis is prevented by interleukin 12 (IL-12) antibody and exacerbated by exogenous IL-12 through an interferon-gamma-dependent mechanism. Hepatology. 2000;32:728-33 pubmed
	Lundberg A, Granger N, Russell J, Callicutt S, Gaber L, Kotb M, et al. Temporal correlation of tumor necrosis factor-alpha release, upregulation of pulmonary ICAM-1 and VCAM-1, neutrophil sequestration, and lung injury in diet-induced pancreatitis. J Gastrointest Surg. 2000;4:248-57 pubmed
	Weng C, Zhao W, Fegeding K, Komisar J, Tseng J. Resistance of Staphylococcal enterotoxin B- induced proliferation and apoptosis to the effects of dexamethasone in mouse lymphocyte cultures. Int Immunol. 1999;11:787-801 pubmed
	Hoffmann K, Caspar P, Cheever A, Wynn T. IFN-gamma, IL-12, and TNF-alpha are required to maintain reduced liver pathology in mice vaccinated with Schistosoma mansoni eggs and IL-12. J Immunol. 1998;161:4201-10 pubmed
	Spaner D, Raju K, Radvanyi L, Lin Y, Miller R. A role for perforin in activation-induced cell death. J Immunol. 1998;160:2655-64 pubmed

product information

Product Type :

Antibody

Product Name :

TNF alpha Monoclonal Antibody (XT22)

Catalog # :

MM350D

Quantity :

500 µg

Price :

US 430.00

Clonality :

Monoclonal

Purity :

protein A

Host :

Rat

Reactivity :

Mouse

Applications :

ELISA: Assay-dependent, Neutralization: Assay-dependent, Western Blot: Assay-dependent

Species :

Mouse

Clone :

XT22

Isotype :

IgG1, kappa

Storage :

-20°C

Description :

TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

Immunogen :

Recombinant mouse TNF alpha

Format :

Liquid

Applications w/Dilutions :

ELISA: Assay-dependent, Neutralization: Assay-dependent, Western Blot: Assay-dependent

Aliases :

APC1 protein; Cachectin; C-domain 1; C-domain 2; cTNF; DADB-70P7.1; DIF; ICD1; ICD2; Intracellular domain 1; Intracellular domain 2; N-terminal fragment; NTF; RATTNF; Tnf; TNF alpha; TNF superfamily; TNF a; TNF, macrophage-derived; TNF, monocyte-derived; TNFA; TNF-a; TNFalpha; TNF-alpha; Tnfsf1a; TNFSF2; TNFa; TNLG1F; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; tumor necrosis factor alpha (cachetin); tumor necrosis factor alpha precursor; tumor necrosis factor ligand 1F; tumor necrosis factor ligand superfamily member 2; Tumor necrosis factor, membrane form; Tumor necrosis factor, soluble form; tumor necrosis factor-alpha; tumor necrosis factor-alpha precursor; tumor-necrosis factor; tumour necrosis factor

more info or order :

Invitrogen product webpage