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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
Zap-70 Monoclonal Antibody (1E7.2), Alexa Fluor 488
catalog :
MHZAP7020
quantity :
500 uL
price :
US 404.00
clonality :
monoclonal
host :
mouse
conjugate :
AF488
clone name :
1E7.2
reactivity :
human, mouse
application :
immunocytochemistry, flow cytometry
more info or order :
Invitrogen product webpage
citations: 24
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; fig 4
Chan A, Punwani D, Kadlecek T, Cowan M, Olson J, Mathes E, et al. A novel human autoimmune syndrome caused by combined hypomorphic and activating mutations in ZAP-70. J Exp Med. 2016;213:155-65 pubmed publisher
  • flow cytometry; human; fig 1
Erdfelder F, Hertweck M, Filipovich A, Uhrmacher S, Kreuzer K. High lymphoid enhancer-binding factor-1 expression is associated with disease progression and poor prognosis in chronic lymphocytic leukemia. Hematol Rep. 2010;2:e3 pubmed publisher
  • flow cytometry; mouse
Deswal S, Schulze A, Hofer T, Schamel W. Quantitative analysis of protein phosphorylations and interactions by multi-colour IP-FCM as an input for kinetic modelling of signalling networks. PLoS ONE. 2011;6:e22928 pubmed publisher
  • flow cytometry; human; tbl 1
Huber S, Oelsner M, Decker T, Zum Büschenfelde C, Wagner M, Lutzny G, et al. Sorafenib induces cell death in chronic lymphocytic leukemia by translational downregulation of Mcl-1. Leukemia. 2011;25:838-47 pubmed publisher
  • flow cytometry; human; fig 2
Zucchetto A, Cattarossi I, Nanni P, Zaina E, Prato G, Gilestro M, et al. Cluster analysis of immunophenotypic data: the example of chronic lymphocytic leukemia. Immunol Lett. 2011;134:137-44 pubmed publisher
  • flow cytometry; human; fig 1
Rossi F, Del Principe M, Rossi D, Irno Consalvo M, Luciano F, Zucchetto A, et al. Prognostic impact of ZAP-70 expression in chronic lymphocytic leukemia: mean fluorescence intensity T/B ratio versus percentage of positive cells. J Transl Med. 2010;8:23 pubmed publisher
  • immunocytochemistry; human
Zum Büschenfelde C, Wagner M, Lutzny G, Oelsner M, Feuerstacke Y, Decker T, et al. Recruitment of PKC-betaII to lipid rafts mediates apoptosis-resistance in chronic lymphocytic leukemia expressing ZAP-70. Leukemia. 2010;24:141-52 pubmed publisher
  • flow cytometry; human
Gachard N, Salviat A, Boutet C, Arnoulet C, Durrieu F, Lenormand B, et al. Multicenter study of ZAP-70 expression in patients with B-cell chronic lymphocytic leukemia using an optimized flow cytometry method. Haematologica. 2008;93:215-23 pubmed publisher
  • flow cytometry; human
Preobrazhensky S, Bahler D. Optimization of flow cytometric measurement of ZAP-70 in chronic lymphocytic leukemia. Cytometry B Clin Cytom. 2008;74:118-27 pubmed
  • flow cytometry; human
Le Garff Tavernier M, Ticchioni M, Brissard M, Salmon C, Raynaud S, Davi F, et al. National standardization of ZAP-70 determination by flow cytometry: the French experience. Cytometry B Clin Cytom. 2007;72:103-8 pubmed
  • flow cytometry; human
Letestu R, Rawstron A, Ghia P, Villamor N, Boeckx N, Leuven N, et al. Evaluation of ZAP-70 expression by flow cytometry in chronic lymphocytic leukemia: A multicentric international harmonization process. Cytometry B Clin Cytom. 2006;70:309-14 pubmed
  • flow cytometry; human
Rassenti L, Kipps T. Clinical utility of assessing ZAP-70 and CD38 in chronic lymphocytic leukemia. Cytometry B Clin Cytom. 2006;70:209-13 pubmed
  • flow cytometry; human
Marti G, Orfao A, Goolsby C. ZAP-70 in CLL: towards standardization of a biomarker for patient management: history of clinical cytometry special issue. Cytometry B Clin Cytom. 2006;70:197-200 pubmed
  • flow cytometry; human
Best O, Ibbotson R, Parker A, Davis Z, Orchard J, Oscier D. ZAP-70 by flow cytometry: a comparison of different antibodies, anticoagulants, and methods of analysis. Cytometry B Clin Cytom. 2006;70:235-41 pubmed
  • flow cytometry; human; fig 1
Passam F, Tachynopoulou V, Skoumi D, Tsompanakou A, Stavropoulos Giokas A, Vadikolia C, et al. Feasibility of an easily applicable method of ZAP-70 measurement in chronic lymphocytic leukemia in the routine flow cytometry setting: A methodological approach. Ann Hematol. 2006;85:795-805 pubmed
  • flow cytometry; human
Del Principe M, Del Poeta G, Buccisano F, Maurillo L, Venditti A, Zucchetto A, et al. Clinical significance of ZAP-70 protein expression in B-cell chronic lymphocytic leukemia. Blood. 2006;108:853-61 pubmed
  • flow cytometry; human; fig 1
Scielzo C, Camporeale A, Geuna M, Alessio M, Poggi A, Zocchi M, et al. ZAP-70 is expressed by normal and malignant human B-cell subsets of different maturational stage. Leukemia. 2006;20:689-95 pubmed
  • flow cytometry; human
Bakke A, Purtzer Z, Leis J, Huang J. A robust ratio metric method for analysis of Zap-70 expression in chronic lymphocytic leukemia (CLL). Cytometry B Clin Cytom. 2006;70:227-34 pubmed
  • flow cytometry; human; tbl 1
Zucchetto A, Bomben R, Dal Bo M, Sonego P, Nanni P, Rupolo M, et al. A scoring system based on the expression of six surface molecules allows the identification of three prognostic risk groups in B-cell chronic lymphocytic leukemia. J Cell Physiol. 2006;207:354-63 pubmed
Wong N, Wong W. Engineering a Dual Small Molecule Gated ZAP70 Switch in T Cells. ACS Synth Biol. 2018;7:969-977 pubmed publisher
McClelland Descalzo D, Satoorian T, Walker L, Sparks N, Pulyanina P, zur Nieden N. Glucose-Induced Oxidative Stress Reduces Proliferation in Embryonic Stem Cells via FOXO3A/?-Catenin-Dependent Transcription of p21(cip1). Stem Cell Reports. 2016;7:55-68 pubmed publisher
Tromp J, Geest C, Breij E, Elias J, van Laar J, Luijks D, et al. Tipping the Noxa/Mcl-1 balance overcomes ABT-737 resistance in chronic lymphocytic leukemia. Clin Cancer Res. 2012;18:487-98 pubmed publisher
Degheidy H, Venzon D, Farooqui M, Abbasi F, Arthur D, Wilson W, et al. Combined normal donor and CLL: Single tube ZAP-70 analysis. Cytometry B Clin Cytom. 2012;82:67-77 pubmed publisher
Zanotti R, Ambrosetti A, Lestani M, Ghia P, Pattaro C, Remo A, et al. ZAP-70 expression, as detected by immunohistochemistry on bone marrow biopsies from early-phase CLL patients, is a strong adverse prognostic factor. Leukemia. 2007;21:102-9 pubmed
product information
Product Type :
Antibody
Product Name :
Zap-70 Monoclonal Antibody (1E7.2), Alexa Fluor 488
Catalog # :
MHZAP7020
Quantity :
500 uL
Price :
US 404.00
Clonality :
Monoclonal
Purity :
purified
Host :
Mouse
Reactivity :
Human, Mouse
Applications :
Flow Cytometry: Assay-Dependent
Species :
Human, Mouse
Clone :
1E7.2
Isotype :
IgG1
Storage :
4 C, store in dark
Description :
ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.
Immunogen :
GST-fusion protein of Human ZAP70, amino acids 282-307
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: Assay-Dependent
Aliases :
70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; EC 2.7.10.2; FLJ17670; FLJ17679; I79_004087; IMD48; kinase ZAP70; mrtle; mur; Selective T cell defect; Srk; STCD; STD; syk-related protein tyrosine kinase; syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; Tyrosine-protein kinase ZAP-70;TZK; TZK; ZA70; Zap70; ZAP-70; Zeta chain associated protein kinase 70kDa; zeta chain of T cell receptor associated protein kinase; zeta chain of T cell receptor associated protein kinase 70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase; zeta-chain (TCR) associated protein kinase 70; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA