Zap-70 Monoclonal Antibody (1E7.2), PE | Invitrogen MHZAP7004 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Zap-70 Monoclonal Antibody (1E7.2), PE

catalog :

MHZAP7004

quantity :

500 µL

price :

US 413.00

clonality :

monoclonal

host :

mouse

conjugate :

clone name :

1E7.2

reactivity :

human, mouse

application :

flow cytometry

more info or order :

Invitrogen product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; fig 2	Vroblova V, Smolej L, Krejsek J. Pitfalls and limitations of ZAP-70 detection in chronic lymphocytic leukemia. Hematology. 2012;17:268-74 pubmed publisher
flow cytometry; human; fig 2	Degheidy H, Venzon D, Farooqui M, Abbasi F, Arthur D, Wilson W, et al. Methodological comparison of two anti-ZAP-70 antibodies. Cytometry B Clin Cytom. 2011;80:300-8 pubmed publisher
flow cytometry; human; fig 2	Vroblova V, Vrbacky F, Hrudkova M, Jankovicova K, Schmitzova D, Maly J, et al. Significant change in ZAP-70 expression during the course of chronic lymphocytic leukemia. Eur J Haematol. 2010;84:513-7 pubmed publisher
flow cytometry; mouse; fig 2	Li F, Ding S, Pan J, Shakhmatov M, Kashentseva E, Wu J, et al. FCRL2 expression predicts IGHV mutation status and clinical progression in chronic lymphocytic leukemia. Blood. 2008;112:179-87 pubmed publisher
flow cytometry; human; 200 ug/ml; fig 2	Bekkema R, Tadema A, Daenen S, Kluin Nelemans H, Mulder A. An improved flow cytometric method using FACS Lysing Solution for measurement of ZAP-70 expression in B-cell chronic lymphocytic leukemia. Cytometry B Clin Cytom. 2008;74:40-4 pubmed
flow cytometry; human	Smolej L, Andrys C, Vroblova V. Modern prognostic factors and angiogenesis in chronic lymphocytic leukemia: more data needed. Leuk Res. 2007;31:1763-4 pubmed
flow cytometry; human	Shankey T, Forman M, Scibelli P, Cobb J, Smith C, Mills R, et al. An optimized whole blood method for flow cytometric measurement of ZAP-70 protein expression in chronic lymphocytic leukemia. Cytometry B Clin Cytom. 2006;70:259-69 pubmed
flow cytometry; human	Marti G, Orfao A, Goolsby C. ZAP-70 in CLL: towards standardization of a biomarker for patient management: history of clinical cytometry special issue. Cytometry B Clin Cytom. 2006;70:197-200 pubmed
flow cytometry; human; fig 1	Passam F, Tachynopoulou V, Skoumi D, Tsompanakou A, Stavropoulos Giokas A, Vadikolia C, et al. Feasibility of an easily applicable method of ZAP-70 measurement in chronic lymphocytic leukemia in the routine flow cytometry setting: A methodological approach. Ann Hematol. 2006;85:795-805 pubmed
	Shah S, Manickam C, Ram D, Kroll K, Itell H, Permar S, et al. CMV Primes Functional Alternative Signaling in Adaptive Δg NK Cells but Is Subverted by Lentivirus Infection in Rhesus Macaques. Cell Rep. 2018;25:2766-2774.e3 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Zap-70 Monoclonal Antibody (1E7.2), PE

Catalog # :

MHZAP7004

Quantity :

500 µL

Price :

US 413.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Human, Mouse

Applications :

Flow Cytometry: Assay-Dependent

Species :

Human, Mouse

Clone :

1E7.2

Isotype :

IgG1

Storage :

4° C, store in dark

Description :

ZAP-70 is a cytosolic protein tyrosine kinase that is a member of the Syk family of proteins. ZAP-70 is expressed exclusively in T-cells and natural killer cells and is required for T-cell receptor activation. Upon T cell antigen receptor (TCR) engagement, ZAP70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains. Phosphorylation of both tyrosines 315 (a Vav binding site) and 319 (a Lck binding site) enhances ZAP70 function in mediating lymphocyte signaling, while tyrosine 292 terminates the transient activation of ZAP70 and attentuates lymphocyte signaling.

Immunogen :

GST-fusion protein of Human ZAP70, amino acids 282-307

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-Dependent

Aliases :

70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; EC 2.7.10.2; FLJ17670; FLJ17679; I79_004087; IMD48; kinase ZAP70; mrtle; mur; Selective T cell defect; Srk; STCD; STD; syk-related protein tyrosine kinase; syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; Tyrosine-protein kinase ZAP-70;TZK; TZK; ZA70; Zap70; ZAP-70; Zeta chain associated protein kinase 70kDa; zeta chain of T cell receptor associated protein kinase; zeta chain of T cell receptor associated protein kinase 70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase; zeta-chain (TCR) associated protein kinase 70; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD

more info or order :

Invitrogen product webpage