Granzyme B Monoclonal Antibody (GB12), APC | Invitrogen MHGB05 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

Granzyme B Monoclonal Antibody (GB12), APC

catalog :

MHGB05

quantity :

500 uL

price :

US 606.00

clonality :

monoclonal

host :

mouse

conjugate :

APC

clone name :

GB12

reactivity :

African green monkey, human, mouse, rhesus macaque

application :

flow cytometry

more info or order :

Invitrogen product webpage

citations: 66

Published Application/Species/Sample/Dilution	Reference
flow cytometry; mouse; 1:100; loading ...; fig s7b	Xu K, Yin N, Peng M, Stamatiades E, Shyu A, Li P, et al. Glycolysis fuels phosphoinositide 3-kinase signaling to bolster T cell immunity. Science. 2021;371:405-410 pubmed publisher
flow cytometry; mouse; 1:100; loading ...	Piersma S, Poursine Laurent J, Yang L, Barber G, Parikh B, Yokoyama W. Virus infection is controlled by hematopoietic and stromal cell sensing of murine cytomegalovirus through STING. elife. 2020;9: pubmed publisher
flow cytometry; mouse; fig 5b	Klein J, Moses K, Zelinskyy G, Sody S, Buer J, Lang S, et al. Combined toll-like receptor 3/7/9 deficiency on host cells results in T-cell-dependent control of tumour growth. Nat Commun. 2017;8:14600 pubmed publisher
flow cytometry; African green monkey; loading ...; fig s4	Nishimura Y, Gautam R, Chun T, Sadjadpour R, Foulds K, Shingai M, et al. Early antibody therapy can induce long-lasting immunity to SHIV. Nature. 2017;543:559-563 pubmed publisher
flow cytometry; rhesus macaque; loading ...; fig 6d	Hippen K, Watkins B, Tkachev V, Lemire A, Lehnen C, Riddle M, et al. Preclinical Testing of Antihuman CD28 Fab' Antibody in a Novel Nonhuman Primate Small Animal Rodent Model of Xenogenic Graft-Versus-Host Disease. Transplantation. 2016;100:2630-2639 pubmed publisher
flow cytometry; human; fig 5	Vogel A, Brown D. Single-Dose CpG Immunization Protects Against a Heterosubtypic Challenge and Generates Antigen-Specific Memory T Cells. Front Immunol. 2015;6:327 pubmed publisher
flow cytometry; mouse	Mouchacca P, Chasson L, Frick M, Foray C, Schmitt Verhulst A, Boyer C. Visualization of granzyme B-expressing CD8 T cells during primary and secondary immune responses to Listeria monocytogenes. Immunology. 2015;145:24-33 pubmed publisher
flow cytometry; human	Sleiman M, Brons N, Kaoma T, Dogu F, Villa Forte A, Lenoble P, et al. NK cell killer Ig-like receptor repertoire acquisition and maturation are strongly modulated by HLA class I molecules. J Immunol. 2014;192:2602-10 pubmed publisher
flow cytometry; mouse; fig 2	Durant L, Makris S, Voorburg C, Loebbermann J, Johansson C, Openshaw P. Regulatory T cells prevent Th2 immune responses and pulmonary eosinophilia during respiratory syncytial virus infection in mice. J Virol. 2013;87:10946-54 pubmed publisher
flow cytometry; mouse; fig 1	Nakajima K, Maekawa Y, Kataoka K, Ishifune C, Nishida J, Arimochi H, et al. The ARNT-STAT3 axis regulates the differentiation of intestinal intraepithelial TCR???CD8??? cells. Nat Commun. 2013;4:2112 pubmed publisher
flow cytometry; mouse	Robb R, Lineburg K, Kuns R, Wilson Y, Raffelt N, Olver S, et al. Identification and expansion of highly suppressive CD8(+)FoxP3(+) regulatory T cells after experimental allogeneic bone marrow transplantation. Blood. 2012;119:5898-908 pubmed publisher
flow cytometry; mouse	Loebbermann J, Schnoeller C, Thornton H, Durant L, Sweeney N, Schuijs M, et al. IL-10 regulates viral lung immunopathology during acute respiratory syncytial virus infection in mice. PLoS ONE. 2012;7:e32371 pubmed publisher
flow cytometry; mouse	Loebbermann J, Thornton H, Durant L, Sparwasser T, Webster K, Sprent J, et al. Regulatory T cells expressing granzyme B play a critical role in controlling lung inflammation during acute viral infection. Mucosal Immunol. 2012;5:161-72 pubmed publisher
flow cytometry; mouse; fig 5	Robb R, Kreijveld E, Kuns R, Wilson Y, Olver S, Don A, et al. Type I-IFNs control GVHD and GVL responses after transplantation. Blood. 2011;118:3399-409 pubmed publisher
flow cytometry; mouse; fig 6	Tate M, Brooks A, Reading P, Mintern J. Neutrophils sustain effective CD8(+) T-cell responses in the respiratory tract following influenza infection. Immunol Cell Biol. 2012;90:197-205 pubmed publisher
flow cytometry; mouse; fig 4	Kallies A, Carotta S, Huntington N, Bernard N, Tarlinton D, Smyth M, et al. A role for Blimp1 in the transcriptional network controlling natural killer cell maturation. Blood. 2011;117:1869-79 pubmed publisher
flow cytometry; mouse; fig 5, 6	Hubert S, Rissiek B, Klages K, Huehn J, Sparwasser T, Haag F, et al. Extracellular NAD+ shapes the Foxp3+ regulatory T cell compartment through the ART2-P2X7 pathway. J Exp Med. 2010;207:2561-8 pubmed publisher
flow cytometry; mouse; fig 4	Klages K, Mayer C, Lahl K, Loddenkemper C, Teng M, Ngiow S, et al. Selective depletion of Foxp3+ regulatory T cells improves effective therapeutic vaccination against established melanoma. Cancer Res. 2010;70:7788-99 pubmed publisher
flow cytometry; rhesus macaque; fig 6	von Gegerfelt A, Valentin A, Alicea C, Van Rompay K, Marthas M, Montefiori D, et al. Emergence of simian immunodeficiency virus-specific cytotoxic CD4+ T cells and increased humoral responses correlate with control of rebounding viremia in CD8-depleted macaques infected with Rev-independent live-attenuated simian immunodeficiency vir. J Immunol. 2010;185:3348-58 pubmed publisher
flow cytometry; human; fig 6	Yuen T, Flesch I, Hollett N, Dobson B, Russell T, Fahrer A, et al. Analysis of A47, an immunoprevalent protein of vaccinia virus, leads to a reevaluation of the total antiviral CD8+ T cell response. J Virol. 2010;84:10220-9 pubmed publisher
flow cytometry; human; fig 3	Antonelli L, Mahnke Y, Hodge J, Porter B, Barber D, DerSimonian R, et al. Elevated frequencies of highly activated CD4+ T cells in HIV+ patients developing immune reconstitution inflammatory syndrome. Blood. 2010;116:3818-27 pubmed publisher
flow cytometry; African green monkey; fig 5	Rout N, Else J, Yue S, Connole M, Exley M, Kaur A. Heterogeneity in phenotype and function of CD8+ and CD4/CD8 double-negative Natural Killer T cell subsets in sooty mangabeys. J Med Primatol. 2010;39:224-34 pubmed publisher
flow cytometry; human; fig 4	Cairo C, Armstrong C, Cummings J, Deetz C, Tan M, Lu C, et al. Impact of age, gender, and race on circulating ?? T cells. Hum Immunol. 2010;71:968-75 pubmed publisher
flow cytometry; mouse; fig s2	Tait E, Jordan K, Dupont C, Harris T, Gregg B, Wilson E, et al. Virulence of Toxoplasma gondii is associated with distinct dendritic cell responses and reduced numbers of activated CD8+ T cells. J Immunol. 2010;185:1502-12 pubmed publisher
flow cytometry; mouse	Zelinskyy G, Dietze K, Hüsecken Y, Schimmer S, Nair S, Werner T, et al. The regulatory T-cell response during acute retroviral infection is locally defined and controls the magnitude and duration of the virus-specific cytotoxic T-cell response. Blood. 2009;114:3199-207 pubmed publisher
flow cytometry; human; fig 1	Hagn M, Schwesinger E, Ebel V, Sontheimer K, Maier J, Beyer T, et al. Human B cells secrete granzyme B when recognizing viral antigens in the context of the acute phase cytokine IL-21. J Immunol. 2009;183:1838-45 pubmed publisher
flow cytometry; mouse	Moffat J, Gebhardt T, Doherty P, Turner S, Mintern J. Granzyme A expression reveals distinct cytolytic CTL subsets following influenza A virus infection. Eur J Immunol. 2009;39:1203-10 pubmed publisher
flow cytometry; mouse	Jenkins M, Mintern J, La Gruta N, Kedzierska K, Doherty P, Turner S. Cell cycle-related acquisition of cytotoxic mediators defines the progressive differentiation to effector status for virus-specific CD8+ T cells. J Immunol. 2008;181:3818-22 pubmed
flow cytometry; human; tbl 1	Lages C, Suffia I, Velilla P, Huang B, Warshaw G, Hildeman D, et al. Functional regulatory T cells accumulate in aged hosts and promote chronic infectious disease reactivation. J Immunol. 2008;181:1835-48 pubmed
flow cytometry; mouse	Sridhar S, Reyes Sandoval A, Draper S, Moore A, Gilbert S, Gao G, et al. Single-dose protection against Plasmodium berghei by a simian adenovirus vector using a human cytomegalovirus promoter containing intron A. J Virol. 2008;82:3822-33 pubmed publisher
flow cytometry; mouse	Zelinskyy G, Balkow S, Schimmer S, Werner T, Simon M, Dittmer U. The level of friend retrovirus replication determines the cytolytic pathway of CD8+ T-cell-mediated pathogen control. J Virol. 2007;81:11881-90 pubmed
flow cytometry; mouse; fig 5	Jenkins M, Kedzierska K, Doherty P, Turner S. Heterogeneity of effector phenotype for acute phase and memory influenza A virus-specific CTL. J Immunol. 2007;179:64-70 pubmed
flow cytometry; human; 1:200; fig 3A	Yang Z, Novak A, Ziesmer S, Witzig T, Ansell S. Attenuation of CD8(+) T-cell function by CD4(+)CD25(+) regulatory T cells in B-cell non-Hodgkin's lymphoma. Cancer Res. 2006;66:10145-52 pubmed
flow cytometry; mouse	Irwin S, Izzo A, Dow S, Skeiky Y, Reed S, Alderson M, et al. Tracking antigen-specific CD8 T lymphocytes in the lungs of mice vaccinated with the Mtb72F polyprotein. Infect Immun. 2005;73:5809-16 pubmed
flow cytometry; human; tbl 2	Bratke K, Kuepper M, Bade B, Virchow J, Luttmann W. Differential expression of human granzymes A, B, and K in natural killer cells and during CD8+ T cell differentiation in peripheral blood. Eur J Immunol. 2005;35:2608-16 pubmed
flow cytometry; mouse; fig 5B	Zelinskyy G, Robertson S, Schimmer S, Messer R, Hasenkrug K, Dittmer U. CD8+ T-cell dysfunction due to cytolytic granule deficiency in persistent Friend retrovirus infection. J Virol. 2005;79:10619-26 pubmed
flow cytometry; human	Grossman W, Radhi M, Schauer D, Gerday E, Grose C, Goldman F. Development of hemophagocytic lymphohistiocytosis in triplets infected with HHV-8. Blood. 2005;106:1203-6 pubmed
flow cytometry; mouse; fig 5	Lang A, Nikolich Zugich J. Development and migration of protective CD8+ T cells into the nervous system following ocular herpes simplex virus-1 infection. J Immunol. 2005;174:2919-25 pubmed
flow cytometry; mouse; 1:200; fig 1	Gondek D, Lu L, Quezada S, Sakaguchi S, Noelle R. Cutting edge: contact-mediated suppression by CD4+CD25+ regulatory cells involves a granzyme B-dependent, perforin-independent mechanism. J Immunol. 2005;174:1783-6 pubmed
flow cytometry; human; 1:400 flow cytometry; mouse; 1:400	Grossman W, Verbsky J, Tollefsen B, Kemper C, Atkinson J, Ley T. Differential expression of granzymes A and B in human cytotoxic lymphocyte subsets and T regulatory cells. Blood. 2004;104:2840-8 pubmed
	Scherer S, Oberle S, Kanev K, Gerullis A, Wu M, de Almeida G, et al. Pyrimidine de novo synthesis inhibition selectively blocks effector but not memory T cell development. Nat Immunol. 2023;24:501-515 pubmed publisher
	Denis M, Math xe9 D, Micoud M, Choffour P, Grasselly C, Matera E, et al. Impact of mouse model tumor implantation site on acquired resistance to anti-PD-1 immune checkpoint therapy. Front Immunol. 2022;13:1011943 pubmed publisher
	Rosati M, Agarwal M, Hu X, Devasundaram S, Stellas D, Chowdhury B, et al. Control of SARS-CoV-2 infection after Spike DNA or Spike DNA+Protein co-immunization in rhesus macaques. PLoS Pathog. 2021;17:e1009701 pubmed publisher
	Tunggal H, MUNSON P, O Connor M, Hajari N, Dross S, Bratt D, et al. Effects of therapeutic vaccination on the control of SIV in rhesus macaques with variable responsiveness to antiretroviral drugs. PLoS ONE. 2021;16:e0253265 pubmed publisher
	Carter T, Agliardi G, Lin F, Ellis M, Jones C, Robson M, et al. Potential of Magnetic Hyperthermia to Stimulate Localized Immune Activation. Small. 2021;17:e2005241 pubmed publisher
	Wong P, Wagner J, Berrien Elliott M, Schappe T, Fehniger T. Flow cytometry-based ex vivo murine NK cell cytotoxicity assay. STAR Protoc. 2021;2:100262 pubmed publisher
	Cubas R, Khan Z, Gong Q, Moskalenko M, Xiong H, Ou Q, et al. Autoimmunity linked protein phosphatase PTPN22 as a target for cancer immunotherapy. J Immunother Cancer. 2020;8: pubmed publisher
	Amor C, Feucht J, Leibold J, Ho Y, Zhu C, Alonso Curbelo D, et al. Senolytic CAR T cells reverse senescence-associated pathologies. Nature. 2020;583:127-132 pubmed publisher
	Wagner J, Wong P, Schappe T, Berrien Elliott M, Cubitt C, Jaeger N, et al. Stage-Specific Requirement for Eomes in Mature NK Cell Homeostasis and Cytotoxicity. Cell Rep. 2020;31:107720 pubmed publisher
	Milner J, Toma C, He Z, Kurd N, Nguyen Q, McDonald B, et al. Heterogenous Populations of Tissue-Resident CD8+ T Cells Are Generated in Response to Infection and Malignancy. Immunity. 2020;52:808-824.e7 pubmed publisher
	Felber B, Lu Z, Hu X, Valentin A, Rosati M, Remmel C, et al. Co-immunization of DNA and Protein in the Same Anatomical Sites Induces Superior Protective Immune Responses against SHIV Challenge. Cell Rep. 2020;31:107624 pubmed publisher
	Leng T, Akther H, Hackstein C, Powell K, King T, Friedrich M, et al. TCR and Inflammatory Signals Tune Human MAIT Cells to Exert Specific Tissue Repair and Effector Functions. Cell Rep. 2019;28:3077-3091.e5 pubmed publisher
	Berrien Elliott M, Sun Y, Neal C, Ireland A, Trissal M, Sullivan R, et al. MicroRNA-142 Is Critical for the Homeostasis and Function of Type 1 Innate Lymphoid Cells. Immunity. 2019;51:479-490.e6 pubmed publisher
	Xiong H, Mittman S, Rodriguez R, Pacheco Sanchez P, Moskalenko M, Yang Y, et al. Coexpression of Inhibitory Receptors Enriches for Activated and Functional CD8+ T Cells in Murine Syngeneic Tumor Models. Cancer Immunol Res. 2019;7:963-976 pubmed publisher
	Cubas R, Moskalenko M, Cheung J, Yang M, McNamara E, Xiong H, et al. Chemotherapy Combines Effectively with Anti-PD-L1 Treatment and Can Augment Antitumor Responses. J Immunol. 2018;201:2273-2286 pubmed publisher
	Hu X, Lu Z, Valentin A, Rosati M, Broderick K, Sardesai N, et al. Gag and env conserved element CE DNA vaccines elicit broad cytotoxic T cell responses targeting subdominant epitopes of HIV and SIV Able to recognize virus-infected cells in macaques. Hum Vaccin Immunother. 2018;14:2163-2177 pubmed publisher
	Hatfield S, Daniels K, O Donnell C, Waggoner S, Welsh R. Weak vaccinia virus-induced NK cell regulation of CD4 T cells is associated with reduced NK cell differentiation and cytolytic activity. Virology. 2018;519:131-144 pubmed publisher
	Delpoux A, Michelini R, Verma S, Lai C, Omilusik K, Utzschneider D, et al. Continuous activity of Foxo1 is required to prevent anergy and maintain the memory state of CD8+ T cells. J Exp Med. 2018;215:575-594 pubmed publisher
	Canter R, Grossenbacher S, Foltz J, Sturgill I, Park J, Luna J, et al. Radiotherapy enhances natural killer cell cytotoxicity and localization in pre-clinical canine sarcomas and first-in-dog clinical trial. J Immunother Cancer. 2017;5:98 pubmed publisher
	Delpoux A, Lai C, Hedrick S, Doedens A. FOXO1 opposition of CD8+ T cell effector programming confers early memory properties and phenotypic diversity. Proc Natl Acad Sci U S A. 2017;114:E8865-E8874 pubmed publisher
	Doedens A, Rubinstein M, Gross E, Best J, Craig D, Baker M, et al. Molecular Programming of Tumor-Infiltrating CD8+ T Cells and IL15 Resistance. Cancer Immunol Res. 2016;4:799-811 pubmed publisher
	van Wilgenburg B, Scherwitzl I, Hutchinson E, Leng T, Kurioka A, Kulicke C, et al. MAIT cells are activated during human viral infections. Nat Commun. 2016;7:11653 pubmed publisher
	Sakala I, Chaudhri G, Eldi P, Buller R, Karupiah G. Deficiency in Th2 cytokine responses exacerbate orthopoxvirus infection. PLoS ONE. 2015;10:e0118685 pubmed publisher
	Kedzierski L, Linossi E, Kolesnik T, Day E, Bird N, Kile B, et al. Suppressor of cytokine signaling 4 (SOCS4) protects against severe cytokine storm and enhances viral clearance during influenza infection. PLoS Pathog. 2014;10:e1004134 pubmed publisher
	Doedens A, Phan A, Stradner M, Fujimoto J, Nguyen J, Yang E, et al. Hypoxia-inducible factors enhance the effector responses of CD8(+) T cells to persistent antigen. Nat Immunol. 2013;14:1173-82 pubmed publisher
	Altin J, Goodnow C, Cook M. IL-10+ CTLA-4+ Th2 inhibitory cells form in a Foxp3-independent, IL-2-dependent manner from Th2 effectors during chronic inflammation. J Immunol. 2012;188:5478-88 pubmed publisher

product information

Product Type :

Antibody

Product Name :

Granzyme B Monoclonal Antibody (GB12), APC

Catalog # :

MHGB05

Quantity :

500 uL

Price :

US 606.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: Assay-dependent

Species :

Human

Clone :

GB12

Isotype :

IgG1

Storage :

4 C

Description :

Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.

Immunogen :

Human Granzyme B.

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-dependent

Aliases :

AI553453; C11; Cathepsin G-like 1; CCP1; CCP-1/C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; Ctla1; Ctla-1; CTSGL1; cytotoxic cell protease 1; cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; cytotoxic T-lymphocyte proteinase 2; cytotoxic T-lymphocyte-associated serine esterase 1; fragmentin; fragmentin 2; fragmentin-2; GLP I; GLP III; GLP-1; granzyme 2; granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); granzyme B(G,H); Granzyme-2; GranzymeB; granzyme-like protein 1; granzyme-like protein I; GRB; GZB; Gzmb; HLP; human lymphocyte protein; Human lymphocyte protein (Hlp); Lymphocyte protease; Natural killer cell protease 1; OTTHUMP00000028189; RNKP-1; SECT; T-cell serine protease 1-3E

more info or order :

Invitrogen product webpage