CD45 Monoclonal Antibody (HI30), Pacific Orange | Invitrogen MHCD4530TR product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD45 Monoclonal Antibody (HI30), Pacific Orange

catalog :

MHCD4530TR

quantity :

25 Tests

price :

US 266.00

clonality :

monoclonal

host :

mouse

conjugate :

pacific orange

clone name :

HI30

reactivity :

human, mouse

application :

immunocytochemistry, flow cytometry

more info or order :

Invitrogen product webpage

citations: 33

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...	Wentink M, Dalm V, Lankester A, van Schouwenburg P, Schölvinck L, Kalina T, et al. Genetic defects in PI3K? affect B-cell differentiation and maturation leading to hypogammaglobulineamia and recurrent infections. Clin Immunol. 2017;176:77-86 pubmed publisher
flow cytometry; human; fig 5	Yawata N, Selva K, Liu Y, Tan K, Lee A, Siak J, et al. Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection. Mucosal Immunol. 2016;9:159-70 pubmed publisher
flow cytometry; human flow cytometry; mouse; fig 6	Billerbeck E, Horwitz J, Labitt R, Donovan B, Vega K, Budell W, et al. Characterization of human antiviral adaptive immune responses during hepatotropic virus infection in HLA-transgenic human immune system mice. J Immunol. 2013;191:1753-64 pubmed publisher
flow cytometry; human; fig 1	Elkins K, Zheng B, Go M, Slaga D, Du C, Scales S, et al. FcRL5 as a target of antibody-drug conjugates for the treatment of multiple myeloma. Mol Cancer Ther. 2012;11:2222-32 pubmed publisher
flow cytometry; human; fig 3	Mokry J, Soukup T, Micuda S, Karbanova J, Visek B, Brcakova E, et al. Telomere attrition occurs during ex vivo expansion of human dental pulp stem cells. J Biomed Biotechnol. 2010;2010:673513 pubmed publisher
flow cytometry; human; fig 1	Streeck H, Cohen K, Jolin J, Brockman M, Meier A, Power K, et al. Rapid ex vivo isolation and long-term culture of human Th17 cells. J Immunol Methods. 2008;333:115-25 pubmed publisher
flow cytometry; human	Jasionowski T, Hartung L, Greenwood J, Perkins S, Bahler D. Analysis of CD10+ hairy cell leukemia. Am J Clin Pathol. 2003;120:228-35 pubmed
flow cytometry; human	Manz M, Miyamoto T, Akashi K, Weissman I. Prospective isolation of human clonogenic common myeloid progenitors. Proc Natl Acad Sci U S A. 2002;99:11872-7 pubmed
flow cytometry; human; fig 6	Janossy G, Jani I, Kahan M, Barnett D, Mandy F, Shapiro H. Precise CD4 T-cell counting using red diode laser excitation: for richer, for poorer. Cytometry. 2002;50:78-85 pubmed
flow cytometry; human	Capmany G, Querol S, Cancelas J, Garcia J. Short-term, serum-free, static culture of cord blood-derived CD34+ cells: effects of FLT3-L and MIP-1alpha on in vitro expansion of hematopoietic progenitor cells. Haematologica. 1999;84:675-82 pubmed
flow cytometry; human; 1 ug	Negrier C, Vinciguerra C, Attali O, Grenier C, Larcher M, Dechavanne M. Illegitimate transcription: its use for studying genetic abnormalities in lymphoblastoid cells from patients with Glanzmann thrombasthenia. Br J Haematol. 1998;100:33-9 pubmed
immunocytochemistry; human; fig 4	Basch R, Quito F, Beh J, Hirst J. Growth of human hematopoietic cells in immunodeficient mice conditioned with cyclophosphamide and busulfan. Stem Cells. 1997;15:314-23 pubmed
	Fernandez R, Mayoral J, Pierre Louis L, Yao Y, Xu Y, Mu S, et al. Improving NK cell function in multiple myeloma with NKTR-255, a novel polymer-conjugated human IL-15. Blood Adv. 2023;7:9-19 pubmed publisher
	Szodoray P, Andersen T, Heinzelbecker J, Imbery J, Huszthy P, Stanford S, et al. Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity. Cell Rep. 2021;36:109525 pubmed publisher
	Montefiori L, Bendig S, Gu Z, Chen X, Pölönen P, Ma X, et al. Enhancer Hijacking Drives Oncogenic BCL11B Expression in Lineage-Ambiguous Stem Cell Leukemia. Cancer Discov. 2021;11:2846-2867 pubmed publisher
	Ehlers A, Den Hartog Jager C, Kardol Hoefnagel T, Katsburg M, Knulst A, Otten H. Comparison of Two Strategies to Generate Antigen-Specific Human Monoclonal Antibodies: Which Method to Choose for Which Purpose?. Front Immunol. 2021;12:660037 pubmed publisher
	Du W, Hua F, Li X, Zhang J, Li S, Wang W, et al. Loss of Optineurin Drives Cancer Immune Evasion via Palmitoylation-Dependent IFNGR1 Lysosomal Sorting and Degradation. Cancer Discov. 2021;11:1826-1843 pubmed publisher
	Xiang J, Rauch D, Huey D, Panfil A, Cheng X, Esser A, et al. HTLV-1 viral oncogene HBZ drives bone destruction in adult T cell leukemia. JCI Insight. 2019;4: pubmed publisher
	Dubay R, Lissandrello C, Swierk P, Moore N, Doty D, Fiering J. Scalable high-throughput acoustophoresis in arrayed plastic microchannels. Biomicrofluidics. 2019;13:034105 pubmed publisher
	Mello F, Land M, Costa E, Teodosio C, Sanchez M, Barcena P, et al. Maturation-associated gene expression profiles during normal human bone marrow erythropoiesis. Cell Death Discov. 2019;5:69 pubmed publisher
	Ickrath P, Scherzad A, Kleinsasser N, Ginzkey C, Hagen R, Hackenberg S. Influence of nasal polyp tissue on the differentiation and activation of T lymphocytes in a co-culture system. Biomed Rep. 2019;10:119-126 pubmed publisher
	Mieczkowska A, Schumacher A, Filipowicz N, Wardowska A, Zielinski M, Madanecki P, et al. Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells. Sci Rep. 2018;8:11339 pubmed publisher
	Edwards V D, Sweeney D, Ho H, Eide C, Rofelty A, Agarwal A, et al. Targeting of colony-stimulating factor 1 receptor (CSF1R) in the CLL microenvironment yields antineoplastic activity in primary patient samples. Oncotarget. 2018;9:24576-24589 pubmed publisher
	Ickrath P, Kleinsasser N, Ding X, Ginzkey C, Beyersdorf N, Hagen R, et al. Accumulation of CD69+ tissue‑resident memory T cells in the nasal polyps of patients with chronic rhinosinusitis. Int J Mol Med. 2018;42:1116-1124 pubmed publisher
	Schoenaker M, Henriet S, Zonderland J, van Deuren M, Pan Hammarstrom Q, Posthumus van Sluijs S, et al. Immunodeficiency in Bloom's Syndrome. J Clin Immunol. 2018;38:35-44 pubmed publisher
	van der Velden V, Flores Montero J, Perez Andres M, Martin Ayuso M, Crespo O, Blanco E, et al. Optimization and testing of dried antibody tube: The EuroFlow LST and PIDOT tubes as examples. J Immunol Methods. 2017;: pubmed publisher
	van Erp F, Knol E, Pontoppidan B, Meijer Y, van der Ent C, Knulst A. The IgE and basophil responses to Ara h 2 and Ara h 6 are good predictors of peanut allergy in children. J Allergy Clin Immunol. 2017;139:358-360.e8 pubmed publisher
	Chao Y, Kaliaperumal N, Chretien A, Tang S, Lee B, Poidinger M, et al. Human plasmacytoid dendritic cells regulate IFN-Î± production through activation-induced splicing of IL-18RÎ±. J Leukoc Biol. 2014;96:1037-46 pubmed publisher
	Li G, Cheng M, Nunoya J, Cheng L, Guo H, Yu H, et al. Plasmacytoid dendritic cells suppress HIV-1 replication but contribute to HIV-1 induced immunopathogenesis in humanized mice. PLoS Pathog. 2014;10:e1004291 pubmed publisher
	Butcher L, Garcia M, Arnold M, Ueno H, Goel A, Boland C. Immune response to JC virus T antigen in patients with and without colorectal neoplasia. Gut Microbes. 2014;5:468-75 pubmed publisher
	Gaur R, Suhosk M, Banaei N. In vitro immunomodulation of a whole blood IFN-? release assay enhances T cell responses in subjects with latent tuberculosis infection. PLoS ONE. 2012;7:e48027 pubmed publisher
	van Dongen J, Lhermitte L, Bottcher S, Almeida J, van der Velden V, Flores Montero J, et al. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012;26:1908-75 pubmed publisher
	Leone A, Rutella S, Bonanno G, Abbate A, Rebuzzi A, Giovannini S, et al. Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function. Eur Heart J. 2005;26:1196-204 pubmed

product information

Product Type :

Antibody

Product Name :

CD45 Monoclonal Antibody (HI30), Pacific Orange

Catalog # :

MHCD4530TR

Quantity :

25 Tests

Price :

US 266.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: Assay-Dependent

Species :

Human

Clone :

HI30

Isotype :

IgG1

Storage :

4 C, store in dark

Description :

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

Immunogen :

CD45 antigen

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-Dependent

Aliases :

B220; cd45; CD45 antigen; CD45 antigen isoform 1 precursor; CD45 antigen isoform 2 precursor; CD45 antigen isoform 3 precursor; CD45 antigen isoform 4 precursor; CD45 antigen isoform 5 precursor; CD45 antigen isoform 6 precursor; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; leukocyte common antigen, CD45; loc; LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase lambda; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

more info or order :

Invitrogen product webpage