CD20 Monoclonal Antibody (HI47), Pacific Orange | Invitrogen MHCD2030 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD20 Monoclonal Antibody (HI47), Pacific Orange

catalog :

MHCD2030

quantity :

500 uL

price :

US 664.00

clonality :

monoclonal

host :

mouse

conjugate :

pacific orange

clone name :

HI47

reactivity :

human

application :

flow cytometry

more info or order :

Invitrogen product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 1c	Szabo P, Dogra P, Gray J, Wells S, Connors T, Weisberg S, et al. Longitudinal profiling of respiratory and systemic immune responses reveals myeloid cell-driven lung inflammation in severe COVID-19. Immunity. 2021;54:797-814.e6 pubmed publisher
flow cytometry; human; loading ...; fig 4a	Neumann L, Mueller M, Moos V, Heller F, Meyer T, Loddenkemper C, et al. Mucosal Inducible NO Synthase-Producing IgA+ Plasma Cells in Helicobacter pylori-Infected Patients. J Immunol. 2016;197:1801-8 pubmed publisher
flow cytometry; human	Llibre A, LÃ³pez MacÃas C, Marafioti T, Mehta H, Partridge A, Kanzig C, et al. LLT1 and CD161 Expression in Human Germinal Centers Promotes B Cell Activation and CXCR4 Downregulation. J Immunol. 2016;196:2085-94 pubmed publisher
flow cytometry; human; fig 1	Gonzalez N, Wennhold K, Balkow S, Kondo E, BÃ¶lck B, Weber T, et al. In vitro and in vivo imaging of initial B-T-cell interactions in the setting of B-cell based cancer immunotherapy. Oncoimmunology. 2015;4:e1038684 pubmed
	Xu Q, Milanez Almeida P, Martins A, Radtke A, Hoehn K, Oguz C, et al. Adaptive immune responses to SARS-CoV-2 persist in the pharyngeal lymphoid tissue of children. Nat Immunol. 2023;24:186-199 pubmed publisher
	Ogishi M, Arias A, Yang R, Han J, Zhang P, Rinchai D, et al. Impaired IL-23-dependent induction of IFN-γ underlies mycobacterial disease in patients with inherited TYK2 deficiency. J Exp Med. 2022;219: pubmed publisher
	Asano T, Boisson B, Onodi F, Matuozzo D, Moncada Velez M, Maglorius Renkilaraj M, et al. X-linked recessive TLR7 deficiency in ~1% of men under 60 years old with life-threatening COVID-19. Sci Immunol. 2021;6: pubmed publisher
	Yu C, Littleton S, Giroux N, Mathew R, Ding S, Kalnitsky J, et al. Mucosal-associated invariant T cell responses differ by sex in COVID-19. Med (N Y). 2021;2:755-772.e5 pubmed publisher
	Shah H, Smith K, Scott E, Larabee J, James J, Ballard J, et al. Human C. difficile toxin-specific memory B cell repertoires encode poorly neutralizing antibodies. JCI Insight. 2020;5: pubmed publisher
	Espinosa J, Mou D, Adams B, Dibernardo L, MacDonald A, McRae M, et al. T Cell Repertoire Maturation Induced by Persistent and Latent Viral Infection Is Insufficient to Induce Costimulation Blockade Resistant Organ Allograft Rejection in Mice. Front Immunol. 2018;9:1371 pubmed publisher
	Vicetti Miguel R, Maryak S, Cherpes T. Brefeldin A, but not monensin, enables flow cytometric detection of interleukin-4 within peripheral T cells responding to ex vivo stimulation with Chlamydia trachomatis. J Immunol Methods. 2012;384:191-5 pubmed publisher

product information

Product Type :

Antibody

Product Name :

CD20 Monoclonal Antibody (HI47), Pacific Orange

Catalog # :

MHCD2030

Quantity :

500 uL

Price :

US 664.00

Clonality :

Monoclonal

Purity :

Affinity chromatography

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: Assay-Dependent

Species :

Human

Clone :

HI47

Isotype :

IgG3

Storage :

4 C, store in dark

Description :

CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.

Immunogen :

CD20 antigen

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-Dependent

Aliases :

AA960661; APY; ATOPY; B1; B-cell differentiation antigen Ly-44; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; Cd20; CD20 antigen; CD20 cell surface protein; CD20 receptor; CVID5; EGK_06167; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; LEU16; LEU-16; Leukocyte surface antigen Leu-16; Ly44; Ly-44; Lymphocyte antigen 44; membrane spanning 4-domains A1; membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; membrane-spanning 4-domains, subfamily A, member 2; MGC3969; MS4A1; MS4A2; S7

more info or order :

Invitrogen product webpage