product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD14 Monoclonal Antibody (TuK4)
catalog :
MHCD1400
quantity :
0.5 mL
price :
US 155.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
TUK4
reactivity :
African green monkey, human, mouse, rhesus macaque
application :
immunocytochemistry, flow cytometry, mass cytometry
more info or order :
citations: 50
Published Application/Species/Sample/DilutionReference
  • flow cytometry; mouse; loading ...; fig s2v
Pasciuto E, Burton O, Roca C, Lagou V, Rajan W, Theys T, et al. Microglia Require CD4 T Cells to Complete the Fetal-to-Adult Transition. Cell. 2020;182:625-640.e24 pubmed publisher
  • flow cytometry; human; loading ...; fig s2c
Banki Z, Krabbendam L, Klaver D, Leng T, Kruis S, Mehta H, et al. Antibody opsonization enhances MAIT cell responsiveness to bacteria via a TNF-dependent mechanism. Immunol Cell Biol. 2019;97:538-551 pubmed publisher
  • flow cytometry; human; 1:100; loading ...; fig 1
Herten M, Zilkens C, Thorey F, Tassemeier T, Lensing Höhn S, Fischer J, et al. Biomechanical Stability and Osteogenesis in a Tibial Bone Defect Treated by Autologous Ovine Cord Blood Cells-A Pilot Study. Molecules. 2019;24: pubmed publisher
  • flow cytometry; human; loading ...; fig 1c
Gorvel L, Korenfeld D, Tung T, Klechevsky E. Dendritic Cell-Derived IL-32?: A Novel Inhibitory Cytokine of NK Cell Function. J Immunol. 2017;199:1290-1300 pubmed publisher
  • mass cytometry; human; loading ...; fig 2a
Chew V, Lai L, Pan L, Lim C, Li J, Ong R, et al. Delineation of an immunosuppressive gradient in hepatocellular carcinoma using high-dimensional proteomic and transcriptomic analyses. Proc Natl Acad Sci U S A. 2017;114:E5900-E5909 pubmed publisher
  • flow cytometry; human; loading ...; tbl 1
Sumatoh H, Teng K, Cheng Y, Newell E. Optimization of mass cytometry sample cryopreservation after staining. Cytometry A. 2017;91:48-61 pubmed publisher
  • flow cytometry; human; loading ...
Cheeseman H, Olejniczak N, Rogers P, Evans A, King D, Ziprin P, et al. Broadly Neutralizing Antibodies Display Potential for Prevention of HIV-1 Infection of Mucosal Tissue Superior to That of Nonneutralizing Antibodies. J Virol. 2017;91: pubmed publisher
  • flow cytometry; human; loading ...
Wahid R, Fresnay S, Levine M, Sztein M. Cross-reactive multifunctional CD4+ T cell responses against Salmonella enterica serovars Typhi, Paratyphi A and Paratyphi B in humans following immunization with live oral typhoid vaccine Ty21a. Clin Immunol. 2016;173:87-95 pubmed publisher
  • flow cytometry; human; loading ...; fig s1
Graves S, Kouriba B, Diarra I, Daou M, Niangaly A, Coulibaly D, et al. Strain-specific Plasmodium falciparum multifunctional CD4(+) T cell cytokine expression in Malian children immunized with the FMP2.1/AS02A vaccine candidate. Vaccine. 2016;34:2546-55 pubmed publisher
  • flow cytometry; human; 1:160; fig 1
Leong M, Newell E. Multiplexed Peptide-MHC Tetramer Staining with Mass Cytometry. Methods Mol Biol. 2015;1346:115-31 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig 2a
Evans T, Li H, Schafer J, Klatt N, Hao X, Traslavina R, et al. SIV-induced Translocation of Bacterial Products in the Liver Mobilizes Myeloid Dendritic and Natural Killer Cells Associated With Liver Damage. J Infect Dis. 2016;213:361-9 pubmed publisher
  • flow cytometry; human
Riou C, Tanko R, Soares A, Masson L, Werner L, Garrett N, et al. Restoration of CD4+ Responses to Copathogens in HIV-Infected Individuals on Antiretroviral Therapy Is Dependent on T Cell Memory Phenotype. J Immunol. 2015;195:2273-2281 pubmed publisher
  • flow cytometry; human
Grieco A, Billett H, Green N, Driscoll M, Bouhassira E. Variation in Gamma-Globin Expression before and after Induction with Hydroxyurea Associated with BCL11A, KLF1 and TAL1. PLoS ONE. 2015;10:e0129431 pubmed publisher
  • flow cytometry; human; fig 1
Tipton C, Fucile C, DARCE J, Chida A, Ichikawa T, Gregoretti I, et al. Diversity, cellular origin and autoreactivity of antibody-secreting cell population expansions in acute systemic lupus erythematosus. Nat Immunol. 2015;16:755-65 pubmed publisher
  • flow cytometry; human
Boer M, Prins C, van Meijgaarden K, van Dissel J, Ottenhoff T, Joosten S. Mycobacterium bovis BCG Vaccination Induces Divergent Proinflammatory or Regulatory T Cell Responses in Adults. Clin Vaccine Immunol. 2015;22:778-88 pubmed publisher
  • flow cytometry; human
Toapanta F, Simon J, Barry E, Pasetti M, Levine M, Kotloff K, et al. Gut-Homing Conventional Plasmablasts and CD27(-) Plasmablasts Elicited after a Short Time of Exposure to an Oral Live-Attenuated Shigella Vaccine Candidate in Humans. Front Immunol. 2014;5:374 pubmed publisher
  • flow cytometry; human
Ilander M, Kreutzman A, Rohon P, Melo T, Faber E, Porkka K, et al. Enlarged memory T-cell pool and enhanced Th1-type responses in chronic myeloid leukemia patients who have successfully discontinued IFN-? monotherapy. PLoS ONE. 2014;9:e87794 pubmed publisher
  • flow cytometry; human; fig 1
Salerno Goncalves R, Rezwan T, Sztein M. B cells modulate mucosal associated invariant T cell immune responses. Front Immunol. 2014;4:511 pubmed publisher
  • flow cytometry; human; fig 3
Pattacini L, Murnane P, Kahle E, Bolton M, Delrow J, Lingappa J, et al. Differential regulatory T cell activity in HIV type 1-exposed seronegative individuals. AIDS Res Hum Retroviruses. 2013;29:1321-9 pubmed publisher
  • flow cytometry; human; fig 1
Kyoizumi S, Kubo Y, Kajimura J, Yoshida K, Imai K, Hayashi T, et al. Age-associated changes in the differentiation potentials of human circulating hematopoietic progenitors to T- or NK-lineage cells. J Immunol. 2013;190:6164-72 pubmed publisher
  • immunocytochemistry; human; fig 1
Parzych E, Li H, Yin X, Liu Q, Wu T, Podsakoff G, et al. Effects of immunosuppression on circulating adeno-associated virus capsid-specific T cells in humans. Hum Gene Ther. 2013;24:431-42 pubmed publisher
  • flow cytometry; human
McArthur M, Sztein M. Unexpected heterogeneity of multifunctional T cells in response to superantigen stimulation in humans. Clin Immunol. 2013;146:140-52 pubmed publisher
  • flow cytometry; human; fig 2
Lelic A, Verschoor C, Ventresca M, Parsons R, Evelegh C, Bowdish D, et al. The polyfunctionality of human memory CD8+ T cells elicited by acute and chronic virus infections is not influenced by age. PLoS Pathog. 2012;8:e1003076 pubmed publisher
  • flow cytometry; human; fig 1
Wong W, Sigvardsson M, Astrand Grundström I, Hogge D, Larsson J, Qian H, et al. Expression of integrin ?2 receptor in human cord blood CD34+CD38-CD90+ stem cells engrafting long-term in NOD/SCID-IL2R?(c) null mice. Stem Cells. 2013;31:360-71 pubmed publisher
  • flow cytometry; human; fig 1
Dale R, Pillai S, Brilot F. Cerebrospinal fluid CD19(+) B-cell expansion in N-methyl-D-aspartate receptor encephalitis. Dev Med Child Neurol. 2013;55:191-3 pubmed publisher
  • flow cytometry; human
Qi Y, Operario D, Georas S, Mosmann T. The acute environment, rather than T cell subset pre-commitment, regulates expression of the human T cell cytokine amphiregulin. PLoS ONE. 2012;7:e39072 pubmed publisher
  • flow cytometry; human
McArthur M, Sztein M. Heterogeneity of multifunctional IL-17A producing S. Typhi-specific CD8+ T cells in volunteers following Ty21a typhoid immunization. PLoS ONE. 2012;7:e38408 pubmed publisher
  • flow cytometry; human; fig 1
Li X, Miao H, Henn A, Topham D, Wu H, Zand M, et al. Ki-67 expression reveals strong, transient influenza specific CD4 T cell responses after adult vaccination. Vaccine. 2012;30:4581-4 pubmed publisher
  • flow cytometry; human; fig 3
Lyke K, Dabo A, Arama C, Daou M, Diarra I, Wang A, et al. Reduced T regulatory cell response during acute Plasmodium falciparum infection in Malian children co-infected with Schistosoma haematobium. PLoS ONE. 2012;7:e31647 pubmed publisher
  • immunocytochemistry; human
Barcena A, Muench M, Kapidzic M, Gormley M, Goldfien G, Fisher S. Human placenta and chorion: potential additional sources of hematopoietic stem cells for transplantation. Transfusion. 2011;51 Suppl 4:94S-105S pubmed publisher
  • flow cytometry; rhesus macaque; fig 1
Vargas Inchaustegui D, Demberg T, Robert Guroff M. A CD8?(-) subpopulation of macaque circulatory natural killer cells can mediate both antibody-dependent and antibody-independent cytotoxic activities. Immunology. 2011;134:326-40 pubmed publisher
  • flow cytometry; human; fig 2
Luiza Silva M, Campi Azevedo A, Batista M, Martins M, Avelar R, da Silveira Lemos D, et al. Cytokine signatures of innate and adaptive immunity in 17DD yellow fever vaccinated children and its association with the level of neutralizing antibody. J Infect Dis. 2011;204:873-83 pubmed publisher
  • flow cytometry; human; fig 2
Dale R, Tantsis E, Merheb V, Brilot F. Cerebrospinal fluid B-cell expansion in longitudinally extensive transverse myelitis associated with neuromyelitis optica immunoglobulin G. Dev Med Child Neurol. 2011;53:856-860 pubmed publisher
  • flow cytometry; human; fig 4
Clement M, Ladell K, Ekeruche Makinde J, Miles J, Edwards E, Dolton G, et al. Anti-CD8 antibodies can trigger CD8+ T cell effector function in the absence of TCR engagement and improve peptide-MHCI tetramer staining. J Immunol. 2011;187:654-63 pubmed publisher
  • flow cytometry; human; fig 1
Scheible K, Zhang G, Baer J, Azadniv M, Lambert K, Pryhuber G, et al. CD8+ T cell immunity to 2009 pandemic and seasonal H1N1 influenza viruses. Vaccine. 2011;29:2159-68 pubmed publisher
  • flow cytometry; human; 1:40; fig 3
Strickland L, Ross J, Williams S, Ross S, Romero M, Spencer S, et al. Preclinical evaluation of carcinoembryonic cell adhesion molecule (CEACAM) 6 as potential therapy target for pancreatic adenocarcinoma. J Pathol. 2009;218:380-90 pubmed publisher
  • flow cytometry; human; fig 1
Sathler Avelar R, Vitelli Avelar D, Massara R, de Lana M, Pinto Dias J, Teixeira Carvalho A, et al. Etiological treatment during early chronic indeterminate Chagas disease incites an activated status on innate and adaptive immunity associated with a type 1-modulated cytokine pattern. Microbes Infect. 2008;10:103-13 pubmed publisher
  • flow cytometry; human; fig 4
Lee D, Sieling P, Ochoa M, Krutzik S, Guo B, Hernandez M, et al. LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells. J Immunol. 2007;179:8128-36 pubmed
  • flow cytometry; human
Siliciano J, Siliciano R. Enhanced culture assay for detection and quantitation of latently infected, resting CD4+ T-cells carrying replication-competent virus in HIV-1-infected individuals. Methods Mol Biol. 2005;304:3-15 pubmed
  • flow cytometry; African green monkey; fig 1
Contamin H, Loizon S, Bourreau E, Michel J, Garraud O, Mercereau Puijalon O, et al. Flow cytometry identification and characterization of mononuclear cell subsets in the neotropical primate Saimiri sciureus (squirrel monkey). J Immunol Methods. 2005;297:61-71 pubmed
  • flow cytometry; human; fig 2
Canonico B, Zamai L, Burattini S, Granger V, Mannello F, Gobbi P, et al. Evaluation of leukocyte stabilisation in TransFix-treated blood samples by flow cytometry and transmission electron microscopy. J Immunol Methods. 2004;295:67-78 pubmed
  • flow cytometry; human
Stacchini A, Demurtas A, Godio L, Martini G, Antinoro V, Palestro G. Flow cytometry in the bone marrow staging of mature B-cell neoplasms. Cytometry B Clin Cytom. 2003;54:10-8 pubmed
  • flow cytometry; human; fig 4
Hertel L, Lacaille V, Strobl H, Mellins E, Mocarski E. Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus. J Virol. 2003;77:7563-74 pubmed
  • flow cytometry; human
Manz M, Miyamoto T, Akashi K, Weissman I. Prospective isolation of human clonogenic common myeloid progenitors. Proc Natl Acad Sci U S A. 2002;99:11872-7 pubmed
  • flow cytometry; human
Hajishengallis G, Martin M, Sojar H, Sharma A, Schifferle R, DeNardin E, et al. Dependence of bacterial protein adhesins on toll-like receptors for proinflammatory cytokine induction. Clin Diagn Lab Immunol. 2002;9:403-11 pubmed
  • flow cytometry; rhesus macaque
Giavedoni L, Velasquillo M, Parodi L, Hubbard G, Hodara V. Cytokine expression, natural killer cell activation, and phenotypic changes in lymphoid cells from rhesus macaques during acute infection with pathogenic simian immunodeficiency virus. J Virol. 2000;74:1648-57 pubmed
  • flow cytometry; human
Le Cleach L, Delaire S, Boumsell L, Bagot M, Bourgault Villada I, Bensussan A, et al. Blister fluid T lymphocytes during toxic epidermal necrolysis are functional cytotoxic cells which express human natural killer (NK) inhibitory receptors. Clin Exp Immunol. 2000;119:225-30 pubmed
  • flow cytometry; human
Lee B, Sharron M, Montaner L, Weissman D, Doms R. Quantification of CD4, CCR5, and CXCR4 levels on lymphocyte subsets, dendritic cells, and differentially conditioned monocyte-derived macrophages. Proc Natl Acad Sci U S A. 1999;96:5215-20 pubmed
  • immunocytochemistry; human; fig 2
Yeaman G, Guyre P, Fanger M, Collins J, White H, Rathbun W, et al. Unique CD8+ T cell-rich lymphoid aggregates in human uterine endometrium. J Leukoc Biol. 1997;61:427-35 pubmed
Canary L, Vinton C, Morcock D, Pierce J, Estes J, Brenchley J, et al. Rate of AIDS progression is associated with gastrointestinal dysfunction in simian immunodeficiency virus-infected pigtail macaques. J Immunol. 2013;190:2959-65 pubmed publisher
product information
Product Type :
Antibody
Product Name :
CD14 Monoclonal Antibody (TuK4)
Catalog # :
MHCD1400
Quantity :
0.5 mL
Price :
US 155.00
Clonality :
Monoclonal
Purity :
purified
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: Assay-Dependent
Species :
Human
Clone :
TuK4
Isotype :
IgG2a
Storage :
4° C, store in dark
Description :
CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.
Immunogen :
Human CD14
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: Assay-Dependent
Aliases :
CD 14; CD14; CD14 antigen; CD14 molecule; cd14 monocyte; lipopolysaccharide receptor; lipoprotein receptor; LPSR antibody; monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14, membrane-bound form; Monocyte differentiation antigen CD14, urinary form; monocyte differentiation antigen CD14; LOW QUALITY PROTEIN: monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein; myeloid membrane glycoprotein precursor; sCD14; soluble CD14
more info or order :
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA