CD8 Monoclonal Antibody (3B5), Pacific Orange | Invitrogen MHCD0830 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD8 Monoclonal Antibody (3B5), Pacific Orange

catalog :

MHCD0830

quantity :

500 uL

price :

US 664.00

clonality :

monoclonal

host :

mouse

conjugate :

pacific orange

clone name :

3B5

reactivity :

African green monkey, human, rhesus macaque

application :

western blot, flow cytometry

more info or order :

Invitrogen product webpage

citations: 36

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 3a	Li N, van Unen V, Höllt T, Thompson A, van Bergen J, Pezzotti N, et al. Mass cytometry reveals innate lymphoid cell differentiation pathways in the human fetal intestine. J Exp Med. 2018;215:1383-1396 pubmed publisher
flow cytometry; human; loading ...; fig 3	Sairafi D, Stikvoort A, Gertow J, Mattsson J, Uhlin M. Donor Cell Composition and Reactivity Predict Risk of Acute Graft-versus-Host Disease after Allogeneic Hematopoietic Stem Cell Transplantation. J Immunol Res. 2016;2016:5601204 pubmed
flow cytometry; human; loading ...; fig s2b	Stikvoort A, Sundin M, Uzunel M, Gertow J, Sundberg B, Schaffer M, et al. Long-Term Stable Mixed Chimerism after Hematopoietic Stem Cell Transplantation in Patients with Non-Malignant Disease, Shall We Be Tolerant?. PLoS ONE. 2016;11:e0154737 pubmed publisher
flow cytometry; rhesus macaque; loading ...; fig 5a	Javed A, Leuchte N, Neumann B, Sopper S, Sauermann U. Noncytolytic CD8+ Cell Mediated Antiviral Response Represents a Strong Element in the Immune Response of Simian Immunodeficiency Virus-Infected Long-Term Non-Progressing Rhesus Macaques. PLoS ONE. 2015;10:e0142086 pubmed publisher
flow cytometry; human; fig 2	Frederiksen J, Buggert M, Noyan K, Nowak P, SÃ¶nnerborg A, Lund O, et al. Multidimensional Clusters of CD4+ T Cell Dysfunction Are Primarily Associated with the CD4/CD8 Ratio in Chronic HIV Infection. PLoS ONE. 2015;10:e0137635 pubmed publisher
flow cytometry; human	Riou C, Tanko R, Soares A, Masson L, Werner L, Garrett N, et al. Restoration of CD4+ Responses to Copathogens in HIV-Infected Individuals on Antiretroviral Therapy Is Dependent on T Cell Memory Phenotype. J Immunol. 2015;195:2273-2281 pubmed publisher
flow cytometry; human	Grieco A, Billett H, Green N, Driscoll M, Bouhassira E. Variation in Gamma-Globin Expression before and after Induction with Hydroxyurea Associated with BCL11A, KLF1 and TAL1. PLoS ONE. 2015;10:e0129431 pubmed publisher
flow cytometry; human; loading ...; fig 1c	Hanley P, Melenhorst J, Nikiforow S, Scheinberg P, Blaney J, Demmler Harrison G, et al. CMV-specific T cells generated from naÃ¯ve T cells recognize atypical epitopes and may be protective in vivo. Sci Transl Med. 2015;7:285ra63 pubmed publisher
flow cytometry; human; fig 5	Valentin A, McKinnon K, Li J, Rosati M, Kulkarni V, Pilkington G, et al. Comparative analysis of SIV-specific cellular immune responses induced by different vaccine platforms in rhesus macaques. Clin Immunol. 2014;155:91-107 pubmed publisher
flow cytometry; human; loading ...; fig 4a	Herrera A, Kim H, Bindra B, Jones K, Alyea E, Armand P, et al. A phase II study of bortezomib plus prednisone for initial therapy of chronic graft-versus-host disease. Biol Blood Marrow Transplant. 2014;20:1737-43 pubmed publisher
flow cytometry; human	Pegram H, Purdon T, van Leeuwen D, Curran K, Giralt S, Barker J, et al. IL-12-secreting CD19-targeted cord blood-derived T cells for the immunotherapy of B-cell acute lymphoblastic leukemia. Leukemia. 2015;29:415-22 pubmed publisher
flow cytometry; rhesus macaque; fig 2 western blot; rhesus macaque; fig 2	Cornwell W, Lewis M, Fan X, Rappaport J, Rogers T. Effect of chronic morphine administration on circulating T cell population dynamics in rhesus macaques. J Neuroimmunol. 2013;265:43-50 pubmed publisher
flow cytometry; human; fig 4	Turcotte S, Gros A, Hogan K, Tran E, Hinrichs C, Wunderlich J, et al. Phenotype and function of T cells infiltrating visceral metastases from gastrointestinal cancers and melanoma: implications for adoptive cell transfer therapy. J Immunol. 2013;191:2217-25 pubmed publisher
flow cytometry; human; loading ...; fig 3	Nikiforow S, Kim H, Bindra B, McDonough S, Glotzbecker B, Armand P, et al. Phase I study of alemtuzumab for therapy of steroid-refractory chronic graft-versus-host disease. Biol Blood Marrow Transplant. 2013;19:804-11 pubmed publisher
flow cytometry; human; fig 4	Yan R, Zhong W, Zhu Y, Zhang X. Trichosanthin-stimulated dendritic cells induce a type 2 helper T lymphocyte response through the OX40 ligand. J Investig Allergol Clin Immunol. 2012;22:491-500 pubmed
flow cytometry; human; fig 1	Wong W, Sigvardsson M, Astrand Grundström I, Hogge D, Larsson J, Qian H, et al. Expression of integrin ?2 receptor in human cord blood CD34+CD38-CD90+ stem cells engrafting long-term in NOD/SCID-IL2R?(c) null mice. Stem Cells. 2013;31:360-71 pubmed publisher
flow cytometry; human; loading ...; fig 2b	Koreth J, Stevenson K, Kim H, McDonough S, Bindra B, Armand P, et al. Bortezomib-based graft-versus-host disease prophylaxis in HLA-mismatched unrelated donor transplantation. J Clin Oncol. 2012;30:3202-8 pubmed publisher
flow cytometry; human; tbl 3	Jankowska M, Marszałł M, Debska Slizien A, Carrero J, Lindholm B, Czarnowski W, et al. Vitamin B6 and the immunity in kidney transplant recipients. J Ren Nutr. 2013;23:57-64 pubmed publisher
flow cytometry; human; loading ...; fig 2a	Man S, Tucky B, Cotleur A, Drazba J, Takeshita Y, Ransohoff R. CXCL12-induced monocyte-endothelial interactions promote lymphocyte transmigration across an in vitro blood-brain barrier. Sci Transl Med. 2012;4:119ra14 pubmed publisher
flow cytometry; human; loading ...; fig 2d	Koreth J, Matsuoka K, Kim H, McDonough S, Bindra B, Alyea E, et al. Interleukin-2 and regulatory T cells in graft-versus-host disease. N Engl J Med. 2011;365:2055-66 pubmed publisher
flow cytometry; human; loading ...; fig 1	Bohmer R, Bandala Sanchez E, Harrison L. Forward light scatter is a simple measure of T-cell activation and proliferation but is not universally suited for doublet discrimination. Cytometry A. 2011;79:646-52 pubmed publisher
flow cytometry; rhesus macaque; loading ...	Lumsden J, Pichyangkul S, Srichairatanakul U, Yongvanitchit K, Limsalakpetch A, Nurmukhambetova S, et al. Evaluation of the safety and immunogenicity in rhesus monkeys of a recombinant malaria vaccine for Plasmodium vivax with a synthetic Toll-like receptor 4 agonist formulated in an emulsion. Infect Immun. 2011;79:3492-500 pubmed publisher
flow cytometry; human; fig 3	Hardie D, Baldwin M, Naylor A, Haworth O, Hou T, Lax S, et al. The stromal cell antigen CD248 (endosialin) is expressed on naive CD8+ human T cells and regulates proliferation. Immunology. 2011;133:288-95 pubmed publisher
flow cytometry; human; loading ...; fig 2a	Hong H, Eberhard J, Keudel P, Bollmann B, Ahmad F, Ballmaier M, et al. Phenotypically and functionally distinct subsets contribute to the expansion of CD56-/CD16+ natural killer cells in HIV infection. AIDS. 2010;24:1823-34 pubmed publisher
flow cytometry; human; 1:1000; fig 1	Schweneker M, Favre D, Martin J, Deeks S, McCune J. HIV-induced changes in T cell signaling pathways. J Immunol. 2008;180:6490-500 pubmed
flow cytometry; African green monkey; fig 1	Contamin H, Loizon S, Bourreau E, Michel J, Garraud O, Mercereau Puijalon O, et al. Flow cytometry identification and characterization of mononuclear cell subsets in the neotropical primate Saimiri sciureus (squirrel monkey). J Immunol Methods. 2005;297:61-71 pubmed
flow cytometry; human; fig 5	Kumar P, Uchil P, Sulochana P, Nirmala G, Chandrashekar R, Haridattatreya M, et al. Screening for T cell-eliciting proteins of Japanese encephalitis virus in a healthy JE-endemic human cohort using recombinant baculovirus-infected insect cell preparations. Arch Virol. 2003;148:1569-91 pubmed
flow cytometry; human	Kim C, Johnston B, Butcher E. Trafficking machinery of NKT cells: shared and differential chemokine receptor expression among V alpha 24(+)V beta 11(+) NKT cell subsets with distinct cytokine-producing capacity. Blood. 2002;100:11-6 pubmed
flow cytometry; human; fig 3	Telford W, Moss M, Morseman J, Allnutt F. Cryptomonad algal phycobiliproteins as fluorochromes for extracellular and intracellular antigen detection by flow cytometry. Cytometry. 2001;44:16-23 pubmed
flow cytometry; human; fig 2	Sharron M, Pohlmann S, Price K, Lolis E, Tsang M, Kirchhoff F, et al. Expression and coreceptor activity of STRL33/Bonzo on primary peripheral blood lymphocytes. Blood. 2000;96:41-9 pubmed
flow cytometry; human; fig 4	Kim C, Pelus L, White J, Applebaum E, Johanson K, Broxmeyer H. CK beta-11/macrophage inflammatory protein-3 beta/EBI1-ligand chemokine is an efficacious chemoattractant for T and B cells. J Immunol. 1998;160:2418-24 pubmed
	Ikumi N, Koenig Z, Mahajan S, Gray C, Tilburgs T. Purification of primary human placental leukocytes to study maternal-fetal interactions. STAR Protoc. 2023;4:102277 pubmed publisher
	Wang J, Huang F, Zhao J, Huang P, Tan J, Huang M, et al. Tumor-Infiltrated CD8+ T Cell 10-Gene Signature Related to Clear Cell Renal Cell Carcinoma Prognosis. Front Immunol. 2022;13:930921 pubmed publisher
	Elias G, Meysman P, Bartholomeus E, De Neuter N, Keersmaekers N, Suls A, et al. Preexisting memory CD4 T cells in naïve individuals confer robust immunity upon hepatitis B vaccination. elife. 2022;11: pubmed publisher
	Kovacsovics Bankowski M, Chisholm L, Vercellini J, Tucker C, Montler R, Haley D, et al. Detailed characterization of tumor infiltrating lymphocytes in two distinct human solid malignancies show phenotypic similarities. J Immunother Cancer. 2014;2:38 pubmed publisher
	D Alessio F, Tsushima K, Aggarwal N, West E, Willett M, Britos M, et al. CD4+CD25+Foxp3+ Tregs resolve experimental lung injury in mice and are present in humans with acute lung injury. J Clin Invest. 2009;119:2898-913 pubmed publisher

product information

Product Type :

Antibody

Product Name :

CD8 Monoclonal Antibody (3B5), Pacific Orange

Catalog # :

MHCD0830

Quantity :

500 uL

Price :

US 664.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: Assay-Dependent

Species :

Human

Clone :

3B5

Isotype :

IgG2a

Storage :

4 C, store in dark

Description :

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

Immunogen :

Human CD8

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-Dependent

Aliases :

BB154331; CD8; CD8 alpha; CD8 alpha chain; CD8 alpha chain precursor; CD8 antigen; CD8 antigen 32 kDa chain; CD8 antigen 37 kDa chain; CD8 antigen alpha polypeptide; CD8 antigen alpha protein; CD8 antigen alpha protein precursor; CD8 antigen alpha-chain; CD8 antigen beta polypeptide; CD8 antigen beta polypeptide precursor; CD8 antigen beta-chain; CD8 antigen, alpha chain; CD8 antigen, alpha polypeptide; CD8 antigen, alpha polypeptide (p32); CD8 antigen, alpha-chain; CD8 antigen, beta chain; CD8 antigen, beta chain 1; CD8 antigen, beta polypeptide; CD8 antigen, beta polypeptide 1 (p37); CD8 antigen, beta-chain; CD8 beta; CD8 beta chain; CD8 beta-2; CD8a; CD8A antigen alpha; CD8a molecule; CD8A; T-cell surface glycoprotein; CD8alpha; CD8B; CD8b antigen; CD8b molecule; CD8b molecule pseudogene; Cd8b1; CD8beta; CD8BP; fCD8; LEU2; Leu-2; Leu2 T-lymphocyte antigen; leu-2a; Ly-2; LY3; Ly-3; Ly-35; Ly-B; Ly-C; Lymphocyte antigen 3; Lyt2; Lyt-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); Lyt3; Lyt-3; MAL; membrane glycoprotein; membrane protein; OKT8 T-cell antigen; OX-8 membrane antigen; p32; P37; RHACD8-4; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T8 T-cell antigen; T-cell antigen Leu2; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-cell surface glycoprotein Lyt-3; T-cell surface molecule; T-lymphocyte differentiation antigen T8/Leu-2; type I transmembrane glycoprotein

more info or order :

Invitrogen product webpage