CD8 Monoclonal Antibody (3B5), Pacific Blue | Invitrogen MHCD0828 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD8 Monoclonal Antibody (3B5), Pacific Blue

catalog :

MHCD0828

quantity :

500 uL

price :

US 516.00

clonality :

monoclonal

host :

mouse

conjugate :

Pacific Blue

clone name :

3B5

reactivity :

human, rhesus macaque

application :

flow cytometry

more info or order :

Invitrogen product webpage

citations: 14

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; loading ...; fig 6	Willemen Y, Van den Bergh J, Bonte S, Anguille S, Heirman C, Stein B, et al. The tumor-associated antigen RHAMM (HMMR/CD168) is expressed by monocyte-derived dendritic cells and presented to T cells. Oncotarget. 2016;7:73960-73970 pubmed publisher
flow cytometry; rhesus macaque; loading ...; fig 3	Cepeda M, Salas M, Folwarczny J, Leandro A, Hodara V, De La Garza M, et al. Establishment of a neonatal rhesus macaque model to study Mycobacterium tuberculosis infection. Tuberculosis (Edinb). 2013;93 Suppl:S51-9 pubmed publisher
flow cytometry; human; fig 5	Phadwal K, Alegre Abarrategui J, Watson A, Pike L, Anbalagan S, Hammond E, et al. A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells. Autophagy. 2012;8:677-89 pubmed publisher
flow cytometry; rhesus macaque; fig 1	Vargas Inchaustegui D, Demberg T, Robert Guroff M. A CD8?(-) subpopulation of macaque circulatory natural killer cells can mediate both antibody-dependent and antibody-independent cytotoxic activities. Immunology. 2011;134:326-40 pubmed publisher
flow cytometry; human; loading ...; fig 1a	Crawford T, Ndhlovu L, Tan A, Carvidi A, Hecht F, Sinclair E, et al. HIV-1 infection abrogates CD8+ T cell mitogen-activated protein kinase signaling responses. J Virol. 2011;85:12343-50 pubmed publisher
flow cytometry; human; loading ...; fig 2a	Comin Anduix B, Sazegar H, Chodon T, Matsunaga D, Jalil J, von Euw E, et al. Modulation of cell signaling networks after CTLA4 blockade in patients with metastatic melanoma. PLoS ONE. 2010;5:e12711 pubmed publisher
	Citrin D, Valle L, Camphausen K, Cooley Zgela T, Smart D, Yao M, et al. Pilot trial of topical MTS‑01 application to reduce dermatitis in patients receiving chemoradiotherapy for stage I‑III carcinoma of the anal canal. Int J Oncol. 2022;60: pubmed publisher
	Sun C, Liu L, P xe9 rez L, Li X, Liu Y, Xu P, et al. Droplet-microfluidics-assisted sequencing of HIV proviruses and their integration sites in cells from people on antiretroviral therapy. Nat Biomed Eng. 2022;6:1004-1012 pubmed publisher
	Ceglia V, Zurawski S, Montes M, Kroll M, Bouteau A, Wang Z, et al. Anti-CD40 Antibody Fused to CD40 Ligand Is a Superagonist Platform for Adjuvant Intrinsic DC-Targeting Vaccines. Front Immunol. 2021;12:786144 pubmed publisher
	Ceglia V, Kelley E, Boyle A, Zurawski S, Mead H, Harms C, et al. A Framework to Identify Antigen-Expanded T Cell Receptor Clusters Within Complex Repertoires. Front Immunol. 2021;12:735584 pubmed publisher
	Yost K, Satpathy A, Wells D, Qi Y, Wang C, Kageyama R, et al. Clonal replacement of tumor-specific T cells following PD-1 blockade. Nat Med. 2019;25:1251-1259 pubmed publisher
	De Waele J, Marcq E, Van Audenaerde J, Van Loenhout J, Deben C, Zwaenepoel K, et al. Poly(I:C) primes primary human glioblastoma cells for an immune response invigorated by PD-L1 blockade. Oncoimmunology. 2018;7:e1407899 pubmed publisher
	Crawford T, Jalbert E, Ndhlovu L, Barbour J. Concomitant evaluation of PMA+ionomycin-induced kinase phosphorylation and cytokine production in T cell subsets by flow cytometry. Cytometry A. 2014;85:268-76 pubmed publisher
	Introini A, Vanpouille C, Lisco A, Grivel J, Margolis L. Interleukin-7 facilitates HIV-1 transmission to cervico-vaginal tissue ex vivo. PLoS Pathog. 2013;9:e1003148 pubmed publisher

product information

Product Type :

Antibody

Product Name :

CD8 Monoclonal Antibody (3B5), Pacific Blue

Catalog # :

MHCD0828

Quantity :

500 uL

Price :

US 516.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: Assay-dependent

Species :

Human

Clone :

3B5

Isotype :

IgG2a

Storage :

4 C

Description :

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

Immunogen :

Recognizes the CD8 antigen

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-dependent

Aliases :

BB154331; CD8; CD8 alpha; CD8 alpha chain; CD8 alpha chain precursor; CD8 antigen; CD8 antigen 32 kDa chain; CD8 antigen 37 kDa chain; CD8 antigen alpha polypeptide; CD8 antigen alpha protein; CD8 antigen alpha protein precursor; CD8 antigen alpha-chain; CD8 antigen beta polypeptide; CD8 antigen beta polypeptide precursor; CD8 antigen beta-chain; CD8 antigen, alpha chain; CD8 antigen, alpha polypeptide; CD8 antigen, alpha polypeptide (p32); CD8 antigen, alpha-chain; CD8 antigen, beta chain; CD8 antigen, beta chain 1; CD8 antigen, beta polypeptide; CD8 antigen, beta polypeptide 1 (p37); CD8 antigen, beta-chain; CD8 beta; CD8 beta chain; CD8 beta-2; CD8a; CD8A antigen alpha; CD8a molecule; CD8A; T-cell surface glycoprotein; CD8alpha; CD8B; CD8b antigen; CD8b molecule; CD8b molecule pseudogene; Cd8b1; CD8beta; CD8BP; fCD8; LEU2; Leu-2; Leu2 T-lymphocyte antigen; leu-2a; Ly-2; LY3; Ly-3; Ly-35; Ly-B; Ly-C; Lymphocyte antigen 3; Lyt2; Lyt-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); Lyt3; Lyt-3; MAL; membrane glycoprotein; membrane protein; OKT8 T-cell antigen; OX-8 membrane antigen; p32; P37; RHACD8-4; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T8 T-cell antigen; T-cell antigen Leu2; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-cell surface glycoprotein Lyt-3; T-cell surface molecule; T-lymphocyte differentiation antigen T8/Leu-2; type I transmembrane glycoprotein

more info or order :

Invitrogen product webpage