CD4 Monoclonal Antibody (S3.5), APC | Invitrogen MHCD0405 product information

product summary

company name :

Invitrogen

other brands :

NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience

product type :

antibody

product name :

CD4 Monoclonal Antibody (S3.5), APC

catalog :

MHCD0405

quantity :

500 uL

price :

US 508.00

clonality :

monoclonal

host :

mouse

conjugate :

APC

clone name :

S3.5

reactivity :

crab eating macaque, human

application :

immunocytochemistry, flow cytometry, immunohistochemistry - frozen section

more info or order :

Invitrogen product webpage

citations: 42

Published Application/Species/Sample/Dilution	Reference
flow cytometry; human; 1:50; fig 4c	Calvanese V, Nguyen A, Bolan T, Vavilina A, Su T, Lee L, et al. MLLT3 governs human haematopoietic stem-cell self-renewal and engraftment. Nature. 2019;576:281-286 pubmed publisher
flow cytometry; human; 1:300; loading ...; fig s1a	Zhu L, Xie X, Zhang L, Wang H, Jie Z, Zhou X, et al. TBK-binding protein 1 regulates IL-15-induced autophagy and NKT cell survival. Nat Commun. 2018;9:2812 pubmed publisher
flow cytometry; human; loading ...; fig 3	Heusinger E, Deppe K, Sette P, Krapp C, Kmiec D, Kluge S, et al. Preadaptation of Simian Immunodeficiency Virus SIVsmm Facilitated Env-Mediated Counteraction of Human Tetherin by Human Immunodeficiency Virus Type 2. J Virol. 2018;92: pubmed publisher
flow cytometry; human; fig 5	Kmiec D, Iyer S, Stürzel C, Sauter D, Hahn B, Kirchhoff F. Vpu-Mediated Counteraction of Tetherin Is a Major Determinant of HIV-1 Interferon Resistance. MBio. 2016;7: pubmed publisher
flow cytometry; human; 1:50; loading ...; fig s2e	Dou D, Calvanese V, Sierra M, Nguyen A, Minasian A, Saarikoski P, et al. Medial HOXA genes demarcate haematopoietic stem cell fate during human development. Nat Cell Biol. 2016;18:595-606 pubmed publisher
flow cytometry; human; 1:40; fig 3	Langer S, Hopfensperger K, Iyer S, Kreider E, Learn G, Lee L, et al. A Naturally Occurring rev1-vpu Fusion Gene Does Not Confer a Fitness Advantage to HIV-1. PLoS ONE. 2015;10:e0142118 pubmed publisher
flow cytometry; human	Reeder J, Kwak Y, McNamara R, Forst C, D Orso I. HIV Tat controls RNA Polymerase II and the epigenetic landscape to transcriptionally reprogram target immune cells. elife. 2015;4: pubmed publisher
flow cytometry; human; loading ...; fig 1b	Martins L, Bonczkowski P, Spivak A, De Spiegelaere W, Novis C, DePaula Silva A, et al. Modeling HIV-1 Latency in Primary T Cells Using a Replication-Competent Virus. AIDS Res Hum Retroviruses. 2016;32:187-93 pubmed publisher
flow cytometry; human immunocytochemistry; human	de Carvalho J, de Castro R, da Silva E, Silveira P, da Silva JanuÃ¡rio M, Arruda E, et al. Nef neutralizes the ability of exosomes from CD4+ T cells to act as decoys during HIV-1 infection. PLoS ONE. 2014;9:e113691 pubmed publisher
flow cytometry; human	Saayman S, Ackley A, Turner A, Famiglietti M, Bosque A, Clemson M, et al. An HIV-encoded antisense long noncoding RNA epigenetically regulates viral transcription. Mol Ther. 2014;22:1164-1175 pubmed publisher
flow cytometry; crab eating macaque; fig 2	Mao C, Brovarney M, Dabbagh K, Birnböck H, Richter W, Del Nagro C. Subcutaneous versus intravenous administration of rituximab: pharmacokinetics, CD20 target coverage and B-cell depletion in cynomolgus monkeys. PLoS ONE. 2013;8:e80533 pubmed publisher
flow cytometry; human; fig 2	Kluge S, Sauter D, Vogl M, Peeters M, Li Y, Bibollet Ruche F, et al. The transmembrane domain of HIV-1 Vpu is sufficient to confer anti-tetherin activity to SIVcpz and SIVgor Vpu proteins: cytoplasmic determinants of Vpu function. Retrovirology. 2013;10:32 pubmed publisher
flow cytometry; human; fig 3	Palin A, Ramachandran V, Acharya S, Lewis D. Human neonatal naive CD4+ T cells have enhanced activation-dependent signaling regulated by the microRNA miR-181a. J Immunol. 2013;190:2682-91 pubmed publisher
flow cytometry; human; fig 3	Sauter D, Unterweger D, Vogl M, Usmani S, Heigele A, Kluge S, et al. Human tetherin exerts strong selection pressure on the HIV-1 group N Vpu protein. PLoS Pathog. 2012;8:e1003093 pubmed publisher
flow cytometry; human; fig 1	Mitchell P, Afzali B, Fazekasova H, Chen D, Ali N, Powell N, et al. Helicobacter pylori induces in-vivo expansion of human regulatory T cells through stimulating interleukin-1β production by dendritic cells. Clin Exp Immunol. 2012;170:300-9 pubmed publisher
flow cytometry; human; fig 3	Turner A, Ackley A, Matrone M, Morris K. Characterization of an HIV-targeted transcriptional gene-silencing RNA in primary cells. Hum Gene Ther. 2012;23:473-83 pubmed publisher
flow cytometry; human; fig 7	Gardam S, Turner V, Anderton H, Limaye S, Basten A, Koentgen F, et al. Deletion of cIAP1 and cIAP2 in murine B lymphocytes constitutively activates cell survival pathways and inactivates the germinal center response. Blood. 2011;117:4041-51 pubmed publisher
flow cytometry; human; fig s2	Yates J, Whittington A, Mitchell P, Lechler R, Lightstone L, Lombardi G. Natural regulatory T cells: number and function are normal in the majority of patients with lupus nephritis. Clin Exp Immunol. 2008;153:44-55 pubmed publisher
flow cytometry; human; loading ...; fig 2b	Grahmann P, Braun R. A new protocol for multiple inhalation of IFN-gamma successfully treats MDR-TB: a case study. Int J Tuberc Lung Dis. 2008;12:636-44 pubmed
flow cytometry; human; fig 1A	Yates J, Rovis F, Mitchell P, Afzali B, Tsang J, Garin M, et al. The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro. Int Immunol. 2007;19:785-99 pubmed
flow cytometry; human; fig 4C	Yaddanapudi K, Palacios G, Towner J, Chen I, Sariol C, Nichol S, et al. Implication of a retrovirus-like glycoprotein peptide in the immunopathogenesis of Ebola and Marburg viruses. FASEB J. 2006;20:2519-30 pubmed publisher
flow cytometry; human; fig 3	Humphreys T, Baldridge L, Billings S, Campbell J, Spinola S. Trafficking pathways and characterization of CD4 and CD8 cells recruited to the skin of humans experimentally infected with Haemophilus ducreyi. Infect Immun. 2005;73:3896-902 pubmed
immunohistochemistry - frozen section; human; fig 1	Kim J, Lim H, Kang S, Hillsamer P, Kim C. Human CD57+ germinal center-T cells are the major helpers for GC-B cells and induce class switch recombination. BMC Immunol. 2005;6:3 pubmed
flow cytometry; human; fig 1	Lee M, Hanspers K, Barker C, Korn A, McCune J. Gene expression profiles during human CD4+ T cell differentiation. Int Immunol. 2004;16:1109-24 pubmed
flow cytometry; human	Braun R, Foerster M, Grahmann P, Haefner D, Workalemahu G, Kroegel C. Phenotypic and molecular characterization of CD103+ CD4+ T cells in bronchoalveolar lavage from patients with interstitial lung diseases. Cytometry B Clin Cytom. 2003;54:19-27 pubmed
flow cytometry; human; fig 1	Litvinova E, Maury S, Boyer O, Bruel S, Benard L, Boisserie G, et al. Graft-versus-leukemia effect after suicide-gene-mediated control of graft-versus-host disease. Blood. 2002;100:2020-5 pubmed
flow cytometry; human	Venkatesan S, Petrovic A, Van Ryk D, Locati M, Weissman D, Murphy P. Reduced cell surface expression of CCR5 in CCR5Delta 32 heterozygotes is mediated by gene dosage, rather than by receptor sequestration. J Biol Chem. 2002;277:2287-301 pubmed
flow cytometry; human	Venkatesan S, Petrovic A, Locati M, Kim Y, Weissman D, Murphy P. A membrane-proximal basic domain and cysteine cluster in the C-terminal tail of CCR5 constitute a bipartite motif critical for cell surface expression. J Biol Chem. 2001;276:40133-45 pubmed
flow cytometry; human	Lee B, Sharron M, Montaner L, Weissman D, Doms R. Quantification of CD4, CCR5, and CXCR4 levels on lymphocyte subsets, dendritic cells, and differentially conditioned monocyte-derived macrophages. Proc Natl Acad Sci U S A. 1999;96:5215-20 pubmed
	Levinger C, Howard J, Cheng J, Tang P, Joshi A, Catalfamo M, et al. An ultrasensitive planar array p24 Gag ELISA to detect HIV-1 in diverse biological matrixes. Sci Rep. 2021;11:23682 pubmed publisher
	Scott T, O Meally D, Grepo N, Soemardy C, Lazar D, Zheng Y, et al. Broadly active zinc finger protein-guided transcriptional activation of HIV-1. Mol Ther Methods Clin Dev. 2021;20:18-29 pubmed publisher
	Nesspor T, Kinealy K, Mazzanti N, Diem M, Boyé K, Hoffman H, et al. High-Throughput Generation of Bipod (Fab × scFv) Bispecific Antibodies Exploits Differential Chain Expression and Affinity Capture. Sci Rep. 2020;10:7557 pubmed publisher
	Buffalo C, Stürzel C, Heusinger E, Kmiec D, Kirchhoff F, Hurley J, et al. Structural Basis for Tetherin Antagonism as a Barrier to Zoonotic Lentiviral Transmission. Cell Host Microbe. 2019;26:359-368.e8 pubmed publisher
	Hotter D, Bosso M, Jønsson K, Krapp C, Stürzel C, Das A, et al. IFI16 Targets the Transcription Factor Sp1 to Suppress HIV-1 Transcription and Latency Reactivation. Cell Host Microbe. 2019;25:858-872.e13 pubmed publisher
	Morton E, Forst C, Zheng Y, DePaula Silva A, Ramirez N, Planelles V, et al. Transcriptional Circuit Fragility Influences HIV Proviral Fate. Cell Rep. 2019;27:154-171.e9 pubmed publisher
	Joas S, Parrish E, Gnanadurai C, Lump E, Stürzel C, Parrish N, et al. Species-specific host factors rather than virus-intrinsic virulence determine primate lentiviral pathogenicity. Nat Commun. 2018;9:1371 pubmed publisher
	Xia Q, Zhou Y, Wang X, Fu S. Interleukin-1 receptor-associated kinase 3 downregulation in peripheral blood mononuclear cells attenuates immunosuppression in sepsis. Exp Ther Med. 2018;15:1586-1593 pubmed publisher
	Yamada E, Nakaoka S, Klein L, Reith E, Langer S, Hopfensperger K, et al. Human-Specific Adaptations in Vpu Conferring Anti-tetherin Activity Are Critical for Efficient Early HIV-1 Replication In Vivo. Cell Host Microbe. 2018;23:110-120.e7 pubmed publisher
	Catakovic K, Gassner F, Ratswohl C, Zaborsky N, Rebhandl S, Schubert M, et al. TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia. Oncoimmunology. 2017;7:e1371399 pubmed publisher
	Manrique S, Sauter D, Horenkamp F, Lülf S, Yu H, Hotter D, et al. Endocytic sorting motif interactions involved in Nef-mediated downmodulation of CD4 and CD3. Nat Commun. 2017;8:442 pubmed publisher
	Vazquez Lombardi R, Loetsch C, Zinkl D, Jackson J, Schofield P, Deenick E, et al. Potent antitumour activity of interleukin-2-Fc fusion proteins requires Fc-mediated depletion of regulatory T-cells. Nat Commun. 2017;8:15373 pubmed publisher
	Gupta P, Wright S, Srivastava S. PEITC treatment suppresses myeloid derived tumor suppressor cells to inhibit breast tumor growth. Oncoimmunology. 2015;4:e981449 pubmed

product information

Product Type :

Antibody

Product Name :

CD4 Monoclonal Antibody (S3.5), APC

Catalog # :

MHCD0405

Quantity :

500 uL

Price :

US 508.00

Clonality :

Monoclonal

Purity :

purified

Host :

Mouse

Reactivity :

Human

Applications :

Flow Cytometry: Assay-Dependent

Species :

Human

Clone :

S3.5

Isotype :

IgG2a

Storage :

4 C, store in dark

Description :

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

Immunogen :

Human CD4

Format :

Liquid

Applications w/Dilutions :

Flow Cytometry: Assay-Dependent

Aliases :

Activation B7-1 antigen; B7; B7.1; B7-1; BB1; B-lymphocyte activation antigen B7; CD28LG; CD28LG1; CD4; CD4 antigen; CD4 antigen (p55); CD4 antigen p55; Cd4 molecule; CD4 precursor; CD4 receptor; CD4, allele 1; cd4a; CD4mut; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 molecule; cell surface glycoprotein CD4; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; fCD4; L3T4; LAB7; Leu-3; Ly-4; lymphocyte antigen CD4; lymphocyte antigen CD4 precursor; membrane protein; p55; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); T-lymphocyte activation antigen CD80; W3/25; W3/25 antigen

more info or order :

Invitrogen product webpage