product summary
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company name :
Invitrogen
other brands :
NeoMarkers, Lab Vision, Endogen, Pierce, BioSource International, Zymed Laboratories, Caltag, Molecular Probes, Research Genetics, Life Technologies, Applied Biosystems, GIBCO BRL, ABgene, Dynal, Affinity BioReagents, Nunc, Invitrogen, NatuTec, Oxoid, Richard-Allan Scientific, Arcturus, Perseptive Biosystems, Proxeon, eBioscience
product type :
antibody
product name :
CD4 Monoclonal Antibody (S3.5)
catalog :
MHCD0400
quantity :
0.5 mL
price :
US 155.00
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
S3.5
reactivity :
African green monkey, sooty mangabey, human, mouse, rat, rhesus macaque
application :
western blot, immunocytochemistry, flow cytometry, immunohistochemistry - frozen section, blocking or activating experiments, other
more info or order :
Invitrogen product webpage
citations: 122
Published Application/Species/Sample/DilutionReference
  • flow cytometry; human; loading ...; fig s1-1
Kasatskaya S, Ladell K, Egorov E, Miners K, Davydov A, Metsger M, et al. Functionally specialized human CD4+ T-cell subsets express physicochemically distinct TCRs. elife. 2020;9: pubmed publisher
  • flow cytometry; human; loading ...; fig 4a
Fenwick C, Loredo Varela J, Joo V, Pellaton C, Farina A, Rajah N, et al. Tumor suppression of novel anti-PD-1 antibodies mediated through CD28 costimulatory pathway. J Exp Med. 2019;: pubmed publisher
  • flow cytometry; human; 1:300; loading ...; fig s1a
Zhu L, Xie X, Zhang L, Wang H, Jie Z, Zhou X, et al. TBK-binding protein 1 regulates IL-15-induced autophagy and NKT cell survival. Nat Commun. 2018;9:2812 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Moysi E, Pallikkuth S, de Armas L, Gonzalez L, Ambrozak D, George V, et al. Altered immune cell follicular dynamics in HIV infection following influenza vaccination. J Clin Invest. 2018;128:3171-3185 pubmed publisher
  • flow cytometry; human; loading ...; fig s4a
Yeo L, Woodwyk A, Sood S, Lorenc A, Eichmann M, Pujol Autonell I, et al. Autoreactive T effector memory differentiation mirrors β cell function in type 1 diabetes. J Clin Invest. 2018;128:3460-3474 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Amodio D, Cotugno N, Macchiarulo G, Rocca S, Dimopoulos Y, Castrucci M, et al. Quantitative Multiplexed Imaging Analysis Reveals a Strong Association between Immunogen-Specific B Cell Responses and Tonsillar Germinal Center Immune Dynamics in Children after Influenza Vaccination. J Immunol. 2018;200:538-550 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Meniailo M, Malashchenko V, Shmarov V, Gazatova N, Melashchenko O, Goncharov A, et al. Direct effects of interleukin-8 on growth and functional activity of T lymphocytes. Int Immunopharmacol. 2017;50:178-185 pubmed publisher
  • flow cytometry; African green monkey; loading ...; fig 2a
Nishimura Y, Gautam R, Chun T, Sadjadpour R, Foulds K, Shingai M, et al. Early antibody therapy can induce long-lasting immunity to SHIV. Nature. 2017;543:559-563 pubmed publisher
  • flow cytometry; human; loading ...; fig s1a
Cheuk S, Schlums H, Gallais Sérézal I, Martini E, Chiang S, Marquardt N, et al. CD49a Expression Defines Tissue-Resident CD8+ T Cells Poised for Cytotoxic Function in Human Skin. Immunity. 2017;46:287-300 pubmed publisher
  • flow cytometry; human; loading ...
Tauriainen J, Scharf L, Frederiksen J, Naji A, Ljunggren H, Sonnerborg A, et al. Perturbed CD8+ T cell TIGIT/CD226/PVR axis despite early initiation of antiretroviral treatment in HIV infected individuals. Sci Rep. 2017;7:40354 pubmed publisher
  • flow cytometry; rhesus macaque; loading ...; fig s1c
Roberts E, Carnathan D, Li H, Shaw G, Silvestri G, Betts M. Collapse of Cytolytic Potential in SIV-Specific CD8+ T Cells Following Acute SIV Infection in Rhesus Macaques. PLoS Pathog. 2016;12:e1006135 pubmed publisher
  • flow cytometry; human; loading ...; fig s1
Riou C, Bunjun R, Müller T, Kiravu A, Ginbot Z, Oni T, et al. Selective reduction of IFN-γ single positive mycobacteria-specific CD4+ T cells in HIV-1 infected individuals with latent tuberculosis infection. Tuberculosis (Edinb). 2016;101:25-30 pubmed publisher
  • flow cytometry; human; loading ...; fig st9
Ferre E, Rose S, Rosenzweig S, Burbelo P, Romito K, Niemela J, et al. Redefined clinical features and diagnostic criteria in autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy. JCI Insight. 2016;1: pubmed
  • flow cytometry; human; loading ...; fig 1c
Demers K, Makedonas G, Buggert M, Eller M, Ratcliffe S, Goonetilleke N, et al. Temporal Dynamics of CD8+ T Cell Effector Responses during Primary HIV Infection. PLoS Pathog. 2016;12:e1005805 pubmed publisher
  • flow cytometry; human; loading ...; fig s1a
Suliman S, Geldenhuys H, Johnson J, Hughes J, Smit E, Murphy M, et al. Bacillus Calmette-Guérin (BCG) Revaccination of Adults with Latent Mycobacterium tuberculosis Infection Induces Long-Lived BCG-Reactive NK Cell Responses. J Immunol. 2016;197:1100-1110 pubmed publisher
  • flow cytometry; rhesus macaque; tbl 1
Williams D, Engle E, Shirk E, Queen S, Gama L, Mankowski J, et al. Splenic Damage during SIV Infection: Role of T-Cell Depletion and Macrophage Polarization and Infection. Am J Pathol. 2016;186:2068-2087 pubmed publisher
  • flow cytometry; human; fig 2
Theorell J, Bryceson Y. Analysis of Intracellular Ca(2+) Mobilization in Human NK Cell Subsets by Flow Cytometry. Methods Mol Biol. 2016;1441:117-30 pubmed publisher
  • flow cytometry; human; fig 1
Paris R, Petrovas C, Ferrando Martinez S, Moysi E, Boswell K, Archer E, et al. Selective Loss of Early Differentiated, Highly Functional PD1high CD4 T Cells with HIV Progression. PLoS ONE. 2015;10:e0144767 pubmed publisher
  • flow cytometry; mouse; loading ...
Okoye Okafor U, Bartholdy B, Cartier J, Gao E, Pietrak B, Rendina A, et al. New IDH1 mutant inhibitors for treatment of acute myeloid leukemia. Nat Chem Biol. 2015;11:878-86 pubmed publisher
  • flow cytometry; human
Riou C, Tanko R, Soares A, Masson L, Werner L, Garrett N, et al. Restoration of CD4+ Responses to Copathogens in HIV-Infected Individuals on Antiretroviral Therapy Is Dependent on T Cell Memory Phenotype. J Immunol. 2015;195:2273-2281 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Martins L, Bonczkowski P, Spivak A, De Spiegelaere W, Novis C, DePaula Silva A, et al. Modeling HIV-1 Latency in Primary T Cells Using a Replication-Competent Virus. AIDS Res Hum Retroviruses. 2016;32:187-93 pubmed publisher
  • flow cytometry; human; fig 4
Marshall M, Pattu V, Halimani M, Maier Peuschel M, Müller M, Becherer U, et al. VAMP8-dependent fusion of recycling endosomes with the plasma membrane facilitates T lymphocyte cytotoxicity. J Cell Biol. 2015;210:135-51 pubmed publisher
  • flow cytometry; human
Grieco A, Billett H, Green N, Driscoll M, Bouhassira E. Variation in Gamma-Globin Expression before and after Induction with Hydroxyurea Associated with BCL11A, KLF1 and TAL1. PLoS ONE. 2015;10:e0129431 pubmed publisher
  • flow cytometry; human
Boer M, Prins C, van Meijgaarden K, van Dissel J, Ottenhoff T, Joosten S. Mycobacterium bovis BCG Vaccination Induces Divergent Proinflammatory or Regulatory T Cell Responses in Adults. Clin Vaccine Immunol. 2015;22:778-88 pubmed publisher
  • flow cytometry; human; tbl s1
Claiborne D, Prince J, Scully E, Macharia G, Micci L, Lawson B, et al. Replicative fitness of transmitted HIV-1 drives acute immune activation, proviral load in memory CD4+ T cells, and disease progression. Proc Natl Acad Sci U S A. 2015;112:E1480-9 pubmed publisher
  • flow cytometry; human; loading ...; fig 1a
Abdel Mohsen M, Wang C, Strain M, Lada S, Deng X, Cockerham L, et al. Select host restriction factors are associated with HIV persistence during antiretroviral therapy. AIDS. 2015;29:411-20 pubmed publisher
  • flow cytometry; human
  • immunocytochemistry; human
de Carvalho J, de Castro R, da Silva E, Silveira P, da Silva Januário M, Arruda E, et al. Nef neutralizes the ability of exosomes from CD4+ T cells to act as decoys during HIV-1 infection. PLoS ONE. 2014;9:e113691 pubmed publisher
  • immunocytochemistry; rat; 1:500
Cavaretta J, Sherer K, Lee K, Kim E, Issema R, Chung H. Polarized axonal surface expression of neuronal KCNQ potassium channels is regulated by calmodulin interaction with KCNQ2 subunit. PLoS ONE. 2014;9:e103655 pubmed publisher
  • flow cytometry; human
Pegram H, Purdon T, van Leeuwen D, Curran K, Giralt S, Barker J, et al. IL-12-secreting CD19-targeted cord blood-derived T cells for the immunotherapy of B-cell acute lymphoblastic leukemia. Leukemia. 2015;29:415-22 pubmed publisher
  • flow cytometry; human
Luciano A, Arbona Ramirez I, Ruiz R, Llorens Bonilla B, Martinez Lopez D, Funderburg N, et al. Alterations in regulatory T cell subpopulations seen in preterm infants. PLoS ONE. 2014;9:e95867 pubmed publisher
  • flow cytometry; human
Deng N, Weaver J, Mosmann T. Cytokine diversity in the Th1-dominated human anti-influenza response caused by variable cytokine expression by Th1 cells, and a minor population of uncommitted IL-2+IFN?- Thpp cells. PLoS ONE. 2014;9:e95986 pubmed publisher
  • flow cytometry; human; fig 1
Wong E, Akilimali N, Govender P, Sullivan Z, Cosgrove C, Pillay M, et al. Low levels of peripheral CD161++CD8+ mucosal associated invariant T (MAIT) cells are found in HIV and HIV/TB co-infection. PLoS ONE. 2013;8:e83474 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Lutwama F, Kagina B, Wajja A, Waiswa F, Mansoor N, Kirimunda S, et al. Distinct T-cell responses when BCG vaccination is delayed from birth to 6 weeks of age in Ugandan infants. J Infect Dis. 2014;209:887-97 pubmed publisher
  • flow cytometry; rhesus macaque; fig 1
  • western blot; rhesus macaque; fig 1
Cornwell W, Lewis M, Fan X, Rappaport J, Rogers T. Effect of chronic morphine administration on circulating T cell population dynamics in rhesus macaques. J Neuroimmunol. 2013;265:43-50 pubmed publisher
  • flow cytometry; human; fig 2
Dintwe O, Day C, Smit E, Nemes E, Gray C, Tameris M, et al. Heterologous vaccination against human tuberculosis modulates antigen-specific CD4+ T-cell function. Eur J Immunol. 2013;43:2409-20 pubmed publisher
  • flow cytometry; human; fig 4
TAN C, Broge T, Seung E, Vrbanac V, Viscidi R, Gordon J, et al. Detection of JC virus-specific immune responses in a novel humanized mouse model. PLoS ONE. 2013;8:e64313 pubmed publisher
  • immunocytochemistry; human; fig 1
Parzych E, Li H, Yin X, Liu Q, Wu T, Podsakoff G, et al. Effects of immunosuppression on circulating adeno-associated virus capsid-specific T cells in humans. Hum Gene Ther. 2013;24:431-42 pubmed publisher
  • flow cytometry; human; fig 1
McArthur M, Sztein M. Unexpected heterogeneity of multifunctional T cells in response to superantigen stimulation in humans. Clin Immunol. 2013;146:140-52 pubmed publisher
  • flow cytometry; human; fig 2
Lelic A, Verschoor C, Ventresca M, Parsons R, Evelegh C, Bowdish D, et al. The polyfunctionality of human memory CD8+ T cells elicited by acute and chronic virus infections is not influenced by age. PLoS Pathog. 2012;8:e1003076 pubmed publisher
  • flow cytometry; human; fig 1
Wong W, Sigvardsson M, Astrand Grundström I, Hogge D, Larsson J, Qian H, et al. Expression of integrin ?2 receptor in human cord blood CD34+CD38-CD90+ stem cells engrafting long-term in NOD/SCID-IL2R?(c) null mice. Stem Cells. 2013;31:360-71 pubmed publisher
  • flow cytometry; human
Qi Y, Operario D, Georas S, Mosmann T. The acute environment, rather than T cell subset pre-commitment, regulates expression of the human T cell cytokine amphiregulin. PLoS ONE. 2012;7:e39072 pubmed publisher
  • flow cytometry; human; fig 1
de Souza M, Ratto Kim S, Chuenarom W, Schuetz A, Chantakulkij S, Nuntapinit B, et al. The Thai phase III trial (RV144) vaccine regimen induces T cell responses that preferentially target epitopes within the V2 region of HIV-1 envelope. J Immunol. 2012;188:5166-76 pubmed publisher
  • flow cytometry; human
St Gelais C, Coleman C, Wang J, Wu L. HIV-1 Nef enhances dendritic cell-mediated viral transmission to CD4+ T cells and promotes T-cell activation. PLoS ONE. 2012;7:e34521 pubmed publisher
  • flow cytometry; human; tbl 3
Jankowska M, Marszałł M, Debska Slizien A, Carrero J, Lindholm B, Czarnowski W, et al. Vitamin B6 and the immunity in kidney transplant recipients. J Ren Nutr. 2013;23:57-64 pubmed publisher
  • flow cytometry; human; loading ...; fig 2a
Man S, Tucky B, Cotleur A, Drazba J, Takeshita Y, Ransohoff R. CXCL12-induced monocyte-endothelial interactions promote lymphocyte transmigration across an in vitro blood-brain barrier. Sci Transl Med. 2012;4:119ra14 pubmed publisher
  • flow cytometry; human; loading ...; fig 1b
Crawford T, Ndhlovu L, Tan A, Carvidi A, Hecht F, Sinclair E, et al. HIV-1 infection abrogates CD8+ T cell mitogen-activated protein kinase signaling responses. J Virol. 2011;85:12343-50 pubmed publisher
  • flow cytometry; human; fig 2
Luiza Silva M, Campi Azevedo A, Batista M, Martins M, Avelar R, da Silveira Lemos D, et al. Cytokine signatures of innate and adaptive immunity in 17DD yellow fever vaccinated children and its association with the level of neutralizing antibody. J Infect Dis. 2011;204:873-83 pubmed publisher
  • flow cytometry; human; fig 1
Barbosa R, Silva S, Silva S, Melo A, Pedro E, Barbosa M, et al. Primary B-cell deficiencies reveal a link between human IL-17-producing CD4 T-cell homeostasis and B-cell differentiation. PLoS ONE. 2011;6:e22848 pubmed publisher
  • flow cytometry; human; fig 2
Clement M, Ladell K, Ekeruche Makinde J, Miles J, Edwards E, Dolton G, et al. Anti-CD8 antibodies can trigger CD8+ T cell effector function in the absence of TCR engagement and improve peptide-MHCI tetramer staining. J Immunol. 2011;187:654-63 pubmed publisher
  • flow cytometry; human
Coleman C, Spearman P, Wu L. Tetherin does not significantly restrict dendritic cell-mediated HIV-1 transmission and its expression is upregulated by newly synthesized HIV-1 Nef. Retrovirology. 2011;8:26 pubmed publisher
  • flow cytometry; human; fig s8
Mamedov I, Britanova O, Bolotin D, Chkalina A, Staroverov D, Zvyagin I, et al. Quantitative tracking of T cell clones after haematopoietic stem cell transplantation. EMBO Mol Med. 2011;3:201-7 pubmed publisher
  • flow cytometry; human; 1:10; fig 7
Watkins N, Sridhar S, Cheng X, Chen G, Toner M, Rodriguez W, et al. A microfabricated electrical differential counter for the selective enumeration of CD4+ T lymphocytes. Lab Chip. 2011;11:1437-47 pubmed publisher
  • flow cytometry; human; fig 1
Antonelli L, Mahnke Y, Hodge J, Porter B, Barber D, DerSimonian R, et al. Elevated frequencies of highly activated CD4+ T cells in HIV+ patients developing immune reconstitution inflammatory syndrome. Blood. 2010;116:3818-27 pubmed publisher
  • flow cytometry; human; fig 1
Schenkel J, Zloza A, Li W, Narasipura S, Al Harthi L. Beta-catenin signaling mediates CD4 expression on mature CD8+ T cells. J Immunol. 2010;185:2013-9 pubmed publisher
  • flow cytometry; human; fig 1
Elsner L, Flügge P, Lozano J, Muppala V, Eiz Vesper B, Demiroglu S, et al. The endogenous danger signals HSP70 and MICA cooperate in the activation of cytotoxic effector functions of NK cells. J Cell Mol Med. 2010;14:992-1002 pubmed publisher
  • flow cytometry; mouse; fig 3
Markley J, Sadelain M. IL-7 and IL-21 are superior to IL-2 and IL-15 in promoting human T cell-mediated rejection of systemic lymphoma in immunodeficient mice. Blood. 2010;115:3508-19 pubmed publisher
  • flow cytometry; human
Amarnath S, Costanzo C, Mariotti J, Ullman J, Telford W, Kapoor V, et al. Regulatory T cells and human myeloid dendritic cells promote tolerance via programmed death ligand-1. PLoS Biol. 2010;8:e1000302 pubmed publisher
  • flow cytometry; human; fig 2
Liang M, Morizono K, Pariente N, Kamata M, Lee B, Chen I. Targeted transduction via CD4 by a lentiviral vector uses a clathrin-mediated entry pathway. J Virol. 2009;83:13026-31 pubmed publisher
  • flow cytometry; human
Alexander L, Zhang S, McAuliffe B, Connors D, Zhou N, Wang T, et al. Inhibition of envelope-mediated CD4+-T-cell depletion by human immunodeficiency virus attachment inhibitors. Antimicrob Agents Chemother. 2009;53:4726-32 pubmed publisher
  • flow cytometry; human
Sivasankar B, Longhi M, Gallagher K, Betts G, Morgan B, Godkin A, et al. CD59 blockade enhances antigen-specific CD4+ T cell responses in humans: a new target for cancer immunotherapy?. J Immunol. 2009;182:5203-7 pubmed publisher
  • flow cytometry; human
Park C, Majeti R, Weissman I. In vivo evaluation of human hematopoiesis through xenotransplantation of purified hematopoietic stem cells from umbilical cord blood. Nat Protoc. 2008;3:1932-40 pubmed publisher
  • flow cytometry; human; 1:100; fig 2
Contreras X, Schweneker M, Chen C, McCune J, Deeks S, Martin J, et al. Suberoylanilide hydroxamic acid reactivates HIV from latently infected cells. J Biol Chem. 2009;284:6782-9 pubmed publisher
  • flow cytometry; human; fig 3b
Sodsai P, Hirankarn N, Avihingsanon Y, Palaga T. Defects in Notch1 upregulation upon activation of T Cells from patients with systemic lupus erythematosus are related to lupus disease activity. Lupus. 2008;17:645-53 pubmed publisher
  • flow cytometry; human; fig 2
Lunemann J, Frey O, Eidner T, Baier M, Roberts S, Sashihara J, et al. Increased frequency of EBV-specific effector memory CD8+ T cells correlates with higher viral load in rheumatoid arthritis. J Immunol. 2008;181:991-1000 pubmed
  • flow cytometry; human; fig s2
Yates J, Whittington A, Mitchell P, Lechler R, Lightstone L, Lombardi G. Natural regulatory T cells: number and function are normal in the majority of patients with lupus nephritis. Clin Exp Immunol. 2008;153:44-55 pubmed publisher
  • flow cytometry; human; loading ...; fig 2b
Grahmann P, Braun R. A new protocol for multiple inhalation of IFN-gamma successfully treats MDR-TB: a case study. Int J Tuberc Lung Dis. 2008;12:636-44 pubmed
  • flow cytometry; human; 1:100; fig 1
Schweneker M, Favre D, Martin J, Deeks S, McCune J. HIV-induced changes in T cell signaling pathways. J Immunol. 2008;180:6490-500 pubmed
  • flow cytometry; human
Carvalho K, Maeda S, Marti L, Yamashita J, Haslett P, Kallas E. Immune cellular parameters of leprosy and human immunodeficiency virus-1 co-infected subjects. Immunology. 2008;124:206-14 pubmed publisher
  • flow cytometry; human; fig 1A
Yamada H, Nakashima Y, Okazaki K, Mawatari T, Fukushi J, Kaibara N, et al. Th1 but not Th17 cells predominate in the joints of patients with rheumatoid arthritis. Ann Rheum Dis. 2008;67:1299-304 pubmed
  • flow cytometry; human
Hiyoshi M, Suzu S, Yoshidomi Y, Hassan R, Harada H, Sakashita N, et al. Interaction between Hck and HIV-1 Nef negatively regulates cell surface expression of M-CSF receptor. Blood. 2008;111:243-50 pubmed
  • flow cytometry; mouse; fig 8
Kang S, Lim H, Andrisani O, Broxmeyer H, Kim C. Vitamin A metabolites induce gut-homing FoxP3+ regulatory T cells. J Immunol. 2007;179:3724-33 pubmed
  • flow cytometry; sooty mangabey
Milush J, Reeves J, Gordon S, Zhou D, Muthukumar A, Kosub D, et al. Virally induced CD4+ T cell depletion is not sufficient to induce AIDS in a natural host. J Immunol. 2007;179:3047-56 pubmed
  • other; mouse
Shi Y, Feng Y, Kang J, Liu C, Li Z, Li D, et al. Critical regulation of CD4+ T cell survival and autoimmunity by beta-arrestin 1. Nat Immunol. 2007;8:817-24 pubmed
  • flow cytometry; human; fig 1A
Yates J, Rovis F, Mitchell P, Afzali B, Tsang J, Garin M, et al. The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro. Int Immunol. 2007;19:785-99 pubmed
  • flow cytometry; human
Zuber J, Viguier M, Lemaitre F, Senée V, Patey N, Elain G, et al. Severe FOXP3+ and naïve T lymphopenia in a non-IPEX form of autoimmune enteropathy combined with an immunodeficiency. Gastroenterology. 2007;132:1694-704 pubmed
  • blocking or activating experiments; human
Thedrez A, de Lalla C, Allain S, Zaccagnino L, Sidobre S, Garavaglia C, et al. CD4 engagement by CD1d potentiates activation of CD4+ invariant NKT cells. Blood. 2007;110:251-8 pubmed
  • flow cytometry; human
Diaz Blanco E, Bruns I, Neumann F, Fischer J, Graef T, Rosskopf M, et al. Molecular signature of CD34(+) hematopoietic stem and progenitor cells of patients with CML in chronic phase. Leukemia. 2007;21:494-504 pubmed
  • flow cytometry; human
De Fanis U, Mori F, Kurnat R, Lee W, Bova M, Adkinson N, et al. GATA3 up-regulation associated with surface expression of CD294/CRTH2: a unique feature of human Th cells. Blood. 2007;109:4343-50 pubmed
  • flow cytometry; human
Clarke S, Betts G, Plant A, Wright K, El Shanawany T, Harrop R, et al. CD4+CD25+FOXP3+ regulatory T cells suppress anti-tumor immune responses in patients with colorectal cancer. PLoS ONE. 2006;1:e129 pubmed
  • flow cytometry; human; tbl 1
Kolar G, Mehta D, Pelayo R, Capra J. A novel human B cell subpopulation representing the initial germinal center population to express AID. Blood. 2007;109:2545-52 pubmed
  • flow cytometry; human; fig 1
Garin M, Chu C, Golshayan D, Cernuda Morollón E, Wait R, Lechler R. Galectin-1: a key effector of regulation mediated by CD4+CD25+ T cells. Blood. 2007;109:2058-65 pubmed
  • flow cytometry; human; fig 4C
  • flow cytometry; African green monkey
Yaddanapudi K, Palacios G, Towner J, Chen I, Sariol C, Nichol S, et al. Implication of a retrovirus-like glycoprotein peptide in the immunopathogenesis of Ebola and Marburg viruses. FASEB J. 2006;20:2519-30 pubmed publisher
  • flow cytometry; human
Kolar G, Mehta D, Wilson P, Capra J. Diversity of the Ig repertoire is maintained with age in spite of reduced germinal centre cells in human tonsil lymphoid tissue. Scand J Immunol. 2006;64:314-24 pubmed
  • flow cytometry; human
Lim H, Broxmeyer H, Kim C. Regulation of trafficking receptor expression in human forkhead box P3+ regulatory T cells. J Immunol. 2006;177:840-51 pubmed
  • flow cytometry; human
Jamieson C, Gotlib J, Durocher J, Chao M, Mariappan M, Lay M, et al. The JAK2 V617F mutation occurs in hematopoietic stem cells in polycythemia vera and predisposes toward erythroid differentiation. Proc Natl Acad Sci U S A. 2006;103:6224-9 pubmed
  • flow cytometry; human; fig 1
Chen L, Cohen A, Lewis D. Impaired allogeneic activation and T-helper 1 differentiation of human cord blood naive CD4 T cells. Biol Blood Marrow Transplant. 2006;12:160-71 pubmed
  • flow cytometry; human
Barsov E, Andersen H, Coalter V, Carrington M, Lifson J, Ott D. Capture of antigen-specific T lymphocytes from human blood by selective immortalization to establish long-term T-cell lines maintaining primary cell characteristics. Immunol Lett. 2006;105:26-37 pubmed
  • flow cytometry; human; fig 3
Olivier A, Lauret E, Gonin P, Galy A. The Notch ligand delta-1 is a hematopoietic development cofactor for plasmacytoid dendritic cells. Blood. 2006;107:2694-701 pubmed
  • flow cytometry; human
Schwendemann J, Choi C, Schirrmacher V, Beckhove P. Dynamic differentiation of activated human peripheral blood CD8+ and CD4+ effector memory T cells. J Immunol. 2005;175:1433-9 pubmed
  • flow cytometry; human; fig 3
Humphreys T, Baldridge L, Billings S, Campbell J, Spinola S. Trafficking pathways and characterization of CD4 and CD8 cells recruited to the skin of humans experimentally infected with Haemophilus ducreyi. Infect Immun. 2005;73:3896-902 pubmed
  • flow cytometry; human
Game D, Rogers N, Lechler R. Acquisition of HLA-DR and costimulatory molecules by T cells from allogeneic antigen presenting cells. Am J Transplant. 2005;5:1614-25 pubmed
  • flow cytometry; human; tbl 2
Lozza L, Lilleri D, Percivalle E, Fornara C, Comolli G, Revello M, et al. Simultaneous quantification of human cytomegalovirus (HCMV)-specific CD4+ and CD8+ T cells by a novel method using monocyte-derived HCMV-infected immature dendritic cells. Eur J Immunol. 2005;35:1795-804 pubmed
  • flow cytometry; African green monkey; fig 1
Contamin H, Loizon S, Bourreau E, Michel J, Garraud O, Mercereau Puijalon O, et al. Flow cytometry identification and characterization of mononuclear cell subsets in the neotropical primate Saimiri sciureus (squirrel monkey). J Immunol Methods. 2005;297:61-71 pubmed
  • flow cytometry; human; fig 3
Shimonkevitz R, Northrop J, Harris L, Craun M, Bar Or D. Interleukin-16 expression in the peripheral blood and CD8 T lymphocytes after traumatic injury. J Trauma. 2005;58:252-8 pubmed
  • immunohistochemistry - frozen section; human
  • flow cytometry; human; fig 3
Kim J, Lim H, Kang S, Hillsamer P, Kim C. Human CD57+ germinal center-T cells are the major helpers for GC-B cells and induce class switch recombination. BMC Immunol. 2005;6:3 pubmed
  • flow cytometry; human
Taylor R, Schols D, Wooley D. Restricted entry of R5 HIV Type 1 strains into eosinophilic cells. AIDS Res Hum Retroviruses. 2004;20:1244-53 pubmed
  • flow cytometry; human; fig 2
Tavano R, Gri G, Molon B, Marinari B, Rudd C, Tuosto L, et al. CD28 and lipid rafts coordinate recruitment of Lck to the immunological synapse of human T lymphocytes. J Immunol. 2004;173:5392-7 pubmed
  • flow cytometry; human
Ku C, Zerboni L, Ito H, Graham B, Wallace M, Arvin A. Varicella-zoster virus transfer to skin by T Cells and modulation of viral replication by epidermal cell interferon-alpha. J Exp Med. 2004;200:917-25 pubmed
  • flow cytometry; human
Viguier M, Lemaitre F, Verola O, Cho M, Gorochov G, Dubertret L, et al. Foxp3 expressing CD4+CD25(high) regulatory T cells are overrepresented in human metastatic melanoma lymph nodes and inhibit the function of infiltrating T cells. J Immunol. 2004;173:1444-53 pubmed
  • flow cytometry; human; fig 1
Fasth A, Cao D, van Vollenhoven R, Trollmo C, Malmstrom V. CD28nullCD4+ T cells--characterization of an effector memory T-cell population in patients with rheumatoid arthritis. Scand J Immunol. 2004;60:199-208 pubmed
  • flow cytometry; human; fig 1
Lee M, Hanspers K, Barker C, Korn A, McCune J. Gene expression profiles during human CD4+ T cell differentiation. Int Immunol. 2004;16:1109-24 pubmed
  • flow cytometry; human; fig 4
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  • flow cytometry; human; fig 1
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  • flow cytometry; human; fig 1
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  • flow cytometry; human; fig 1
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  • flow cytometry; human; fig 3
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  • flow cytometry; human; 2 ug
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product information
Product Type :
Antibody
Product Name :
CD4 Monoclonal Antibody (S3.5)
Catalog # :
MHCD0400
Quantity :
0.5 mL
Price :
US 155.00
Clonality :
Monoclonal
Purity :
purified
Host :
Mouse
Reactivity :
Human
Applications :
Flow Cytometry: Assay-Dependent
Species :
Human
Clone :
S3.5
Isotype :
IgG2a
Storage :
4° C, store in dark
Description :
The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].
Immunogen :
Human CD4
Format :
Liquid
Applications w/Dilutions :
Flow Cytometry: Assay-Dependent
Aliases :
Activation B7-1 antigen; B7; B7.1; B7-1; BB1; B-lymphocyte activation antigen B7; CD28LG; CD28LG1; CD4; CD4 antigen; CD4 antigen (p55); CD4 antigen p55; Cd4 molecule; CD4 precursor; CD4 receptor; CD4, allele 1; cd4a; CD4mut; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 molecule; cell surface glycoprotein CD4; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; fCD4; L3T4; LAB7; Leu-3; Ly-4; lymphocyte antigen CD4; lymphocyte antigen CD4 precursor; membrane protein; p55; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); T-lymphocyte activation antigen CD80; W3/25; W3/25 antigen
more info or order :
Invitrogen product webpage
company information
Invitrogen
Thermo Fisher Scientific
81 Wyman Street
Waltham, MA USA 02451
https://www.thermofisher.com
800-678-5599
headquarters: USA